FastQCFastQC Report
Tue 7 Mar 2017
1NF_30A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_30A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2701047
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA158180.5856247595839688No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT64610.23920353847970807No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG62680.23205816115010217No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA62000.2295406188785312No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA61900.2291703920738884No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG59290.21950747247271152No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50050.1852985157237175No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC49790.18433592603164625No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG48460.1794119095298971No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG47770.17685734457786184No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG47350.17530239199836212No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA47340.17526536931789785No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC44440.16452879198325687No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA42690.158049822902008No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT41270.15279260227608035No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA39970.14797965381572406No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA39440.14601745175111724No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG38590.1428705239116535No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA37540.1389831424629042No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA36470.13502171565322632No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA33480.12395193419440685No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32840.12158248264469297No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC31660.11721380634990802No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC29320.10855049912126669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAG355.5479177E-1088.999883
GCTGCAA850.088.999883
AGCTGCA1400.088.999882
GCAGCTA355.5479177E-1088.999883
CTGCTGC2150.088.999871
CAGCAGC5950.088.999871
GCTGCTA600.088.999873
GCTGCAT500.088.999873
GCTGCAC450.088.999873
TGCTGCC450.088.999872
TGCTGCA750.088.999872
GCAGCGG450.088.999873
GCAGCCG158.577962E-488.999873
GCAGCAC950.088.999873
TGCAGCG256.816317E-788.999872
AGCAGCA2050.088.999862
CAGCTGC6500.088.3152541
AGCTGCC3450.087.7100142
GCTGCCC2500.087.219873
AGCAGCG1400.085.821312
Produced by FastQC (version 0.11.5)