##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_30A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2701047
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.805077808716398	12.0	12.0	12.0	2.0	32.0
2	14.56742292896051	12.0	12.0	12.0	12.0	22.0
3	13.721578706331286	12.0	12.0	12.0	12.0	22.0
4	13.718857539317161	12.0	12.0	12.0	12.0	22.0
5	22.420451772960632	22.0	22.0	22.0	22.0	27.0
6	26.740126699017086	27.0	22.0	32.0	22.0	32.0
7	34.90782463244808	37.0	37.0	37.0	27.0	41.0
8	37.5441689833609	41.0	37.0	41.0	27.0	41.0
9	38.503760948994966	41.0	37.0	41.0	32.0	41.0
10-11	38.864346307191255	41.0	41.0	41.0	37.0	41.0
12-13	39.0470874812619	41.0	41.0	41.0	37.0	41.0
14-15	39.00182096053864	41.0	41.0	41.0	37.0	41.0
16-17	38.900686844767975	41.0	41.0	41.0	37.0	41.0
18-19	38.83714444806033	41.0	41.0	41.0	37.0	41.0
20-21	38.854118606599584	41.0	41.0	41.0	37.0	41.0
22-23	38.842496261634835	41.0	41.0	41.0	34.5	41.0
24-25	38.85724443151119	41.0	41.0	41.0	37.0	41.0
26-27	38.827864528088554	41.0	41.0	41.0	37.0	41.0
28-29	38.77293638355793	41.0	41.0	41.0	34.5	41.0
30-31	38.643058784241816	41.0	41.0	41.0	32.0	41.0
32-33	38.69235633441403	41.0	41.0	41.0	32.0	41.0
34-35	38.6680013342974	41.0	41.0	41.0	32.0	41.0
36-37	38.59370088710045	41.0	41.0	41.0	32.0	41.0
38-39	38.49222597755611	41.0	41.0	41.0	32.0	41.0
40-41	38.54069366434572	41.0	41.0	41.0	32.0	41.0
42-43	38.27561071688127	41.0	41.0	41.0	32.0	41.0
44-45	38.38993120075289	41.0	41.0	41.0	32.0	41.0
46-47	38.38977922264959	41.0	41.0	41.0	32.0	41.0
48-49	38.34652710597039	41.0	41.0	41.0	32.0	41.0
50-51	38.22980514593045	41.0	41.0	41.0	32.0	41.0
52-53	38.210624435635516	41.0	41.0	41.0	32.0	41.0
54-55	38.077902198665925	41.0	37.0	41.0	32.0	41.0
56-57	37.859913766772664	41.0	37.0	41.0	27.0	41.0
58-59	37.85900041724561	41.0	37.0	41.0	29.5	41.0
60-61	37.85403345443452	41.0	37.0	41.0	29.5	41.0
62-63	37.85123009706977	41.0	37.0	41.0	29.5	41.0
64-65	37.84126137012795	41.0	37.0	41.0	29.5	41.0
66-67	37.705508086308754	41.0	37.0	41.0	27.0	41.0
68-69	37.73116665500452	41.0	37.0	41.0	27.0	41.0
70-71	37.719861594411356	41.0	37.0	41.0	27.0	41.0
72-73	37.5503412195345	41.0	37.0	41.0	27.0	41.0
74-75	37.47956088879609	41.0	37.0	41.0	27.0	41.0
76-77	36.447131427183606	39.0	34.5	41.0	24.5	41.0
78-79	36.95389898805907	41.0	37.0	41.0	27.0	41.0
80-81	37.61920840325992	41.0	37.0	41.0	27.0	41.0
82-83	37.786524077515125	41.0	37.0	41.0	27.0	41.0
84-85	37.70931383274708	41.0	37.0	41.0	27.0	41.0
86-87	37.57612122262219	41.0	37.0	41.0	27.0	41.0
88-89	37.47628604759562	41.0	37.0	41.0	27.0	41.0
90-91	37.30824917152496	41.0	37.0	41.0	27.0	41.0
92-93	37.2082148144775	41.0	37.0	41.0	27.0	41.0
94-95	37.047163192643445	41.0	37.0	41.0	27.0	41.0
96-97	36.989727872191786	41.0	37.0	41.0	27.0	41.0
98-99	36.87016682790044	41.0	37.0	41.0	27.0	41.0
100	36.731654799046446	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3520341814564922
1101	2	-0.5232617583811869
1101	3	-0.044108619594975806
1101	4	0.06910147465675998
1101	5	-1.026834393142586
1101	6	-0.8071038738334373
1101	7	-0.2711497705499113
1101	8	-0.17416373726095458
1101	9	-0.0354414833223089
1101	10-11	0.016373310394442342
1101	12-13	-0.09683413078701619
1101	14-15	0.09609188172817085
1101	16-17	0.03396566204659024
1101	18-19	0.12856565966378497
1101	20-21	0.16529046696827265
1101	22-23	0.21533725390251846
1101	24-25	0.13066188288861014
1101	26-27	0.22291983476371513
1101	28-29	0.04784365159917314
1101	30-31	0.014044633480125412
1101	32-33	-0.07690582977374305
1101	34-35	0.14653270580667765
1101	36-37	0.06917444655275062
1101	38-39	0.054814843041874894
1101	40-41	0.18452543206380057
1101	42-43	0.15632130126308397
1101	44-45	0.07414142890583264
1101	46-47	0.15571323336093457
1101	48-49	0.14730027492174003
1101	50-51	-0.03758380673737349
1101	52-53	-0.015095248830000685
1101	54-55	-0.04410531868276735
1101	56-57	0.2178711569395233
1101	58-59	0.0912997938055824
1101	60-61	0.10507338186356208
1101	62-63	-0.024957401838967996
1101	64-65	0.13209599325760024
1101	66-67	0.09760299326030264
1101	68-69	0.04069162163747109
1101	70-71	0.013966151698149076
1101	72-73	0.13896900659416644
1101	74-75	0.07620150904897827
1101	76-77	-0.24573054938223038
1101	78-79	0.05748991255075708
1101	80-81	0.14173540628807046
1101	82-83	0.16379829722344397
1101	84-85	0.16243592571112941
1101	86-87	-0.10908669678911309
1101	88-89	-0.2669879657400003
1101	90-91	-0.08913323174083132
1101	92-93	-0.018547943320101012
1101	94-95	0.23203860072579374
1101	96-97	0.24337540564050641
1101	98-99	0.0951667308225197
1101	100	-0.2833974161110717
1102	1	1.4618103047174102
1102	2	0.6501322201819626
1102	3	-0.08756350628039122
1102	4	0.1250966840153076
1102	5	-0.7543199045045696
1102	6	-0.006754279808188102
1102	7	0.07133239643196276
1102	8	-0.15336276925214776
1102	9	-0.09043223180181315
1102	10-11	-0.1660878992954551
1102	12-13	-0.11577800183774656
1102	14-15	-0.08630236978438433
1102	16-17	-0.09508674124031558
1102	18-19	-0.14358077954621962
1102	20-21	-0.0912617744368589
1102	22-23	-0.04747050449473278
1102	24-25	-0.08012087143733737
1102	26-27	-0.08361073124725493
1102	28-29	-0.18568106390797112
1102	30-31	-0.11717098649525326
1102	32-33	-0.1711215430847659
1102	34-35	-0.025828493596890212
1102	36-37	-0.20163150582631317
1102	38-39	0.05613124610152198
1102	40-41	-0.11142362467280265
1102	42-43	-0.013489664951350733
1102	44-45	0.09136988262009282
1102	46-47	-0.23583719161617722
1102	48-49	-0.1763920512829813
1102	50-51	-0.03669694170865512
1102	52-53	-0.1821242306934181
1102	54-55	-0.1811269801383375
1102	56-57	0.07258964411875723
1102	58-59	0.0854416149303816
1102	60-61	0.049480362663764765
1102	62-63	-0.09459752324940496
1102	64-65	-0.18378436915286755
1102	66-67	-0.09872852787951558
1102	68-69	-0.20660283572517102
1102	70-71	-0.23315301462579185
1102	72-73	-0.14904528558027152
1102	74-75	-0.32852732621220326
1102	76-77	0.12289424257544823
1102	78-79	-0.004297135038250133
1102	80-81	-0.16432495275950032
1102	82-83	-0.12247402371284011
1102	84-85	-0.12075084949306358
1102	86-87	-0.15960386269559734
1102	88-89	-0.15321605369851454
1102	90-91	-0.2847992291785957
1102	92-93	-0.17674700301203927
1102	94-95	-0.06566883597226791
1102	96-97	-0.14332412434660569
1102	98-99	-0.3241888933203185
1102	100	-0.47331850779578133
1103	1	1.4906611429293442
1103	2	0.683150472835214
1103	3	0.36525772170187665
1103	4	0.9452769585341283
1103	5	2.904748639950018
1103	6	-0.10094937151600547
1103	7	-0.35792830461203096
1103	8	-0.3433644870757462
1103	9	-0.3128600196383431
1103	10-11	-0.20129633130303404
1103	12-13	-0.43418017343021376
1103	14-15	-0.49706847703328805
1103	16-17	-0.3685249258428698
1103	18-19	-0.2974893587670664
1103	20-21	-0.2628783891389048
1103	22-23	-0.1585163663601321
1103	24-25	-0.2683855567710225
1103	26-27	-0.3089134496344954
1103	28-29	-0.18420883968863677
1103	30-31	-0.09693560990313443
1103	32-33	-0.25587281265519835
1103	34-35	-0.32806199357192156
1103	36-37	-0.32341843020676464
1103	38-39	-0.19952645806291258
1103	40-41	-0.2866103488051479
1103	42-43	-0.10083331144630137
1103	44-45	-0.4862309233483444
1103	46-47	-0.4192671822550409
1103	48-49	-0.39771944412984084
1103	50-51	-0.24525801472822906
1103	52-53	-0.23286323328369463
1103	54-55	-0.33618233009779175
1103	56-57	-0.28972542229187326
1103	58-59	-0.5566992323353404
1103	60-61	-0.27715912982638713
1103	62-63	-0.34748850694398925
1103	64-65	-0.5483372712594274
1103	66-67	-0.47292630517310386
1103	68-69	-0.5462493994651183
1103	70-71	-0.650927805573609
1103	72-73	-0.48427023723332496
1103	74-75	-0.5283231082692339
1103	76-77	-0.3633385629524639
1103	78-79	-0.3637712066039853
1103	80-81	-0.37135791283665753
1103	82-83	-0.4275698312536633
1103	84-85	-0.4341449890253415
1103	86-87	-0.32117587435484296
1103	88-89	-0.08048305489258212
1103	90-91	-0.515765460132755
1103	92-93	-0.3821739169355354
1103	94-95	-0.35530349934744976
1103	96-97	-0.3024329159994892
1103	98-99	-0.201454513303716
1103	100	-0.22929224393771364
1104	1	2.201930329461602
1104	2	-0.4698677869881891
1104	3	-0.0738891318805539
1104	4	-0.021995380545449805
1104	5	-1.1478966680525247
1104	6	-0.5571628619054323
1104	7	-0.2941578097690325
1104	8	-0.3726650669449896
1104	9	-0.101977250581335
1104	10-11	-0.24948276148784032
1104	12-13	-0.17072860577060567
1104	14-15	-0.2802940341252338
1104	16-17	-0.09788435494232317
1104	18-19	-0.04061516920413055
1104	20-21	-0.24096780720481092
1104	22-23	-0.028627168173237294
1104	24-25	-0.07930535637760272
1104	26-27	-0.1270803996259673
1104	28-29	-0.18066294403989502
1104	30-31	-0.16412382464719855
1104	32-33	-0.12158189367288941
1104	34-35	-0.26849087466425203
1104	36-37	-0.2325891709239727
1104	38-39	-0.25176131563751625
1104	40-41	-0.1344390030336058
1104	42-43	-0.15055965082435563
1104	44-45	-0.08285851501425867
1104	46-47	-0.28578249232604236
1104	48-49	-0.5691867306006557
1104	50-51	-0.3999151959095144
1104	52-53	-0.3664914908514234
1104	54-55	-0.38346729952913705
1104	56-57	-0.2718169120523015
1104	58-59	-0.2504107717350692
1104	60-61	-0.09248110028367762
1104	62-63	-0.2922120856828627
1104	64-65	-0.3970110393185706
1104	66-67	-0.6641577698708545
1104	68-69	-0.6586806827725766
1104	70-71	-0.32129218654145575
1104	72-73	-0.3354781766407555
1104	74-75	-0.2091365130041396
1104	76-77	-0.41841263935349104
1104	78-79	-0.10741287041880554
1104	80-81	-0.4113094112777631
1104	82-83	-0.4057598816832524
1104	84-85	-0.24033740782591906
1104	86-87	-0.29185900248396734
1104	88-89	-0.16376083640081163
1104	90-91	-0.2686681817611287
1104	92-93	-0.3342053135129248
1104	94-95	-0.28077713303807883
1104	96-97	-0.27521699225109586
1104	98-99	-0.2827312589646809
1104	100	-0.12127753325035684
1105	1	1.6865162525826563
1105	2	0.264814643164792
1105	3	0.06374171283477281
1105	4	-0.280693749721042
1105	5	-1.1382293332624975
1105	6	0.15141163688387493
1105	7	-0.055551635664919274
1105	8	-0.06743761584582586
1105	9	-0.03575338122795557
1105	10-11	-0.06600823172875181
1105	12-13	-0.19511910587223014
1105	14-15	-0.1214932668308748
1105	16-17	-0.174780282634444
1105	18-19	-0.1499647704572169
1105	20-21	-0.11946654819288938
1105	22-23	-0.06202157740052172
1105	24-25	-0.13975385978769594
1105	26-27	-0.17125768615110815
1105	28-29	-0.1263789859508222
1105	30-31	-0.21040614618709697
1105	32-33	-0.13028853487979575
1105	34-35	-0.0686712487141321
1105	36-37	-0.0993714767951559
1105	38-39	-0.16253620976863914
1105	40-41	-0.231482185137871
1105	42-43	-0.05049371967111682
1105	44-45	-0.12049380649350638
1105	46-47	-0.41350449395103084
1105	48-49	-0.39865210877955093
1105	50-51	-0.28963203683738925
1105	52-53	-0.3829183444735804
1105	54-55	-0.27638869912532016
1105	56-57	-0.013748880415853648
1105	58-59	-0.09257349913496427
1105	60-61	-0.28070736493720716
1105	62-63	-0.44475587098890657
1105	64-65	-0.4175856382475942
1105	66-67	-0.22110488436750586
1105	68-69	-0.28229502999376876
1105	70-71	-0.5299666499842388
1105	72-73	-0.41163513620269043
1105	74-75	-0.41948525309061324
1105	76-77	-0.30039281133821305
1105	78-79	-0.2449570557585261
1105	80-81	-0.550175633536476
1105	82-83	-0.3651102444884202
1105	84-85	-0.2805125192882585
1105	86-87	-0.35018076226803174
1105	88-89	-0.3997112945488013
1105	90-91	-0.37207408779890727
1105	92-93	-0.17959754697713493
1105	94-95	-0.3033849885091513
1105	96-97	-0.390708642401421
1105	98-99	-0.5459063289355655
1105	100	-0.5204024311192228
1106	1	1.3747855887993676
1106	2	-0.6906100164389013
1106	3	-0.09694726971108736
1106	4	-0.14787746860838702
1106	5	-1.0714710573029933
1106	6	-0.646307840750822
1106	7	-0.12124000973577154
1106	8	-0.1929083097311377
1106	9	-0.26522731611693473
1106	10-11	-0.07993889984829394
1106	12-13	-0.18964029548983063
1106	14-15	-0.12076082378272446
1106	16-17	-0.06470995131630275
1106	18-19	-0.01057669698700181
1106	20-21	-0.13246798055704545
1106	22-23	-0.07306321317717135
1106	24-25	-0.3309144139615512
1106	26-27	-0.10257545767782972
1106	28-29	-0.14471305481620078
1106	30-31	-0.12571018436295844
1106	32-33	-0.2858986744513672
1106	34-35	-0.11966066754356319
1106	36-37	-0.3423074841080478
1106	38-39	-0.14468979515516622
1106	40-41	-0.24946323358926747
1106	42-43	-0.03558774304774914
1106	44-45	-0.16526812521131262
1106	46-47	-0.39036198630662255
1106	48-49	-0.22074485715624803
1106	50-51	-0.23778786798627038
1106	52-53	-0.15885083242297782
1106	54-55	-0.41451584802124586
1106	56-57	-0.3316879126188681
1106	58-59	-0.188459456231179
1106	60-61	-0.34594550247214073
1106	62-63	-0.5124967991161213
1106	64-65	-0.5900939855455505
1106	66-67	-0.43685185038522434
1106	68-69	-0.4964219313033169
1106	70-71	-0.7049687831437481
1106	72-73	-0.5427419172719823
1106	74-75	-0.5192334528438636
1106	76-77	-0.49792679152010777
1106	78-79	-0.4331237555299623
1106	80-81	-0.48371154125529614
1106	82-83	-0.5188738022907486
1106	84-85	-0.7614769556725491
1106	86-87	-0.6399374352106122
1106	88-89	-0.6245092388824247
1106	90-91	-0.7529782187147589
1106	92-93	-0.6926781860017783
1106	94-95	-0.6741451591339356
1106	96-97	-0.6347363203209113
1106	98-99	-0.8304351874364571
1106	100	-0.9403841209172654
1107	1	1.2207746147380583
1107	2	-0.5893436913890202
1107	3	-0.02256342697573288
1107	4	-0.1667958206852962
1107	5	-1.3672917311647765
1107	6	-0.5283366816722825
1107	7	-0.6351021310853113
1107	8	-0.525962001006306
1107	9	-0.4680462214316492
1107	10-11	-0.33359077949639726
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2222	5	3.2202192517229733
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2222	9	0.14467943655912308
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2222	28-29	0.20049173448681046
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2222	62-63	0.3671664384209308
2222	64-65	0.25406868108951386
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2222	82-83	0.16088556147767719
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2222	86-87	0.4579964781795809
2222	88-89	0.16756045158970778
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2222	94-95	0.4641995004579158
2222	96-97	0.4931051194952829
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2222	100	0.605283506779358
2223	1	1.5043791376282947
2223	2	-0.9489444911683904
2223	3	-0.0919804843392189
2223	4	-0.02296136122583725
2223	5	-1.1456235706573281
2223	6	0.12079735031889172
2223	7	0.19458864387331687
2223	8	0.058119631646754044
2223	9	0.11299107862350866
2223	10-11	0.07354949215280016
2223	12-13	0.12846903884793193
2223	14-15	0.10461412485444299
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2223	20-21	0.16852708861156174
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2223	26-27	0.05726857053748802
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2223	36-37	0.0400334623376466
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2223	50-51	0.26774425090155773
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2223	54-55	0.19349024703421946
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2223	60-61	0.24549436877836683
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2223	64-65	0.2717138147557776
2223	66-67	0.4312047514501387
2223	68-69	0.35008334194692026
2223	70-71	0.22044040737809922
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2223	76-77	0.42692613958521264
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2223	80-81	0.2862605516086987
2223	82-83	0.21658195719425066
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2223	86-87	0.3371872737333703
2223	88-89	0.2314406309984065
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2223	94-95	0.37589404782401203
2223	96-97	0.32002227439214437
2223	98-99	0.17575779982556838
2223	100	0.5349459708509912
2224	1	1.3590399669015731
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2224	3	-0.004963006229445455
2224	4	-0.39992296389695525
2224	5	-1.0264470519697184
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2224	7	0.2756168882261534
2224	8	0.063353524870152
2224	9	-0.08984902903891623
2224	10-11	0.13656182563376973
2224	12-13	0.11832509145261128
2224	14-15	0.1857476467106025
2224	16-17	0.1754624277046659
2224	18-19	0.1783157944136491
2224	20-21	0.09973249898940395
2224	22-23	0.11028032078883854
2224	24-25	0.16477646065049356
2224	26-27	0.10501430322121053
2224	28-29	0.13821129617146255
2224	30-31	0.0710141520590497
2224	32-33	0.10978036555938786
2224	34-35	0.20064771835687623
2224	36-37	-0.028433199786299213
2224	38-39	-0.047966439114340176
2224	40-41	-0.01427574269280285
2224	42-43	0.27033564996745696
2224	44-45	0.20978649602541566
2224	46-47	0.10295391491576567
2224	48-49	-0.012038512129301182
2224	50-51	0.13537601813265354
2224	52-53	0.10724667628840479
2224	54-55	0.17846980480056374
2224	56-57	0.30970966275844347
2224	58-59	0.2956909314395446
2224	60-61	0.2600666150391504
2224	62-63	0.2621310762018112
2224	64-65	0.25280168229010513
2224	66-67	0.3393842001912404
2224	68-69	0.2860707018324433
2224	70-71	0.33671072184778694
2224	72-73	0.32329319718051863
2224	74-75	0.2550306310319428
2224	76-77	0.3546221295003775
2224	78-79	0.19435100665757687
2224	80-81	0.08074677013618015
2224	82-83	0.12353146487712507
2224	84-85	0.23963612709403037
2224	86-87	0.359603886455254
2224	88-89	0.30864277061886014
2224	90-91	0.3561333038452119
2224	92-93	0.2561550203488494
2224	94-95	0.4659061428122655
2224	96-97	0.2700791719424842
2224	98-99	0.26667156766993116
2224	100	0.5541187417957616
2225	1	1.4175438167028585
2225	2	1.5688157669690934
2225	3	0.8364811692708791
2225	4	1.1417493152207818
2225	5	3.199495601705994
2225	6	-0.15239227761051666
2225	7	0.34116283345134946
2225	8	0.46099806419336886
2225	9	0.1955322026525863
2225	10-11	0.20896023346643489
2225	12-13	0.22967356955997076
2225	14-15	0.16556085073305837
2225	16-17	0.13471654316196435
2225	18-19	0.11153851143863847
2225	20-21	0.12916827418113996
2225	22-23	0.22533998964741642
2225	24-25	0.07805303679683817
2225	26-27	0.057875326687373274
2225	28-29	0.07679849597335675
2225	30-31	0.07238835219973083
2225	32-33	-0.015480018155386688
2225	34-35	0.038996031832333244
2225	36-37	0.10135575164456867
2225	38-39	0.034284711608201235
2225	40-41	0.08025851230704006
2225	42-43	0.17914573550800839
2225	44-45	0.17998999329674348
2225	46-47	0.2697488175856577
2225	48-49	0.20370597780433997
2225	50-51	0.23739030981411702
2225	52-53	0.22215548926521222
2225	54-55	0.28049072937874797
2225	56-57	0.44470441766780056
2225	58-59	0.39058506360495215
2225	60-61	0.30035823119375493
2225	62-63	0.2940295268931976
2225	64-65	0.3261468936107903
2225	66-67	0.3751662969485352
2225	68-69	0.3281727586426939
2225	70-71	0.3334204649267818
2225	72-73	0.33933528308026695
2225	74-75	0.3421008363320013
2225	76-77	0.4501683264355094
2225	78-79	0.37151720868132543
2225	80-81	0.15622828442310066
2225	82-83	0.2913412007449736
2225	84-85	0.2562045928744112
2225	86-87	0.37157677525524235
2225	88-89	0.4281345750797101
2225	90-91	0.3141293798915399
2225	92-93	0.23578233318890796
2225	94-95	0.4734167928633539
2225	96-97	0.5045483293128896
2225	98-99	0.5308370540211342
2225	100	0.5190705155343878
2226	1	1.409348411583231
2226	2	1.38129853541027
2226	3	0.7434282268528154
2226	4	1.1002521868947746
2226	5	3.060825105467398
2226	6	-0.5055127424914652
2226	7	0.007568943799981298
2226	8	0.14937876727126564
2226	9	0.11617967500382065
2226	10-11	-0.04526926211470794
2226	12-13	0.1570397087515758
2226	14-15	-0.03637458785492953
2226	16-17	-0.03847211072128687
2226	18-19	0.09575667584967107
2226	20-21	0.08473004067218426
2226	22-23	0.13020234420800847
2226	24-25	0.12013843665483392
2226	26-27	0.0415539476375173
2226	28-29	0.04736699350262086
2226	30-31	0.1074753406433544
2226	32-33	-0.04375568068041957
2226	34-35	0.08039368419971282
2226	36-37	0.1124144990150171
2226	38-39	0.16340324352483293
2226	40-41	0.17401334093066367
2226	42-43	0.3317095389839082
2226	44-45	0.27283741939419315
2226	46-47	0.3396890007224158
2226	48-49	0.23652950947354867
2226	50-51	0.25965550363065404
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2226	54-55	0.3232944264507225
2226	56-57	0.469777661260693
2226	58-59	0.40443948937618757
2226	60-61	0.3566631386407124
2226	62-63	0.28126302474057496
2226	64-65	0.3646728719846166
2226	66-67	0.444529065066547
2226	68-69	0.3013213191700288
2226	70-71	0.39812124427295004
2226	72-73	0.427337422065861
2226	74-75	0.5734816636286126
2226	76-77	0.5882692344439775
2226	78-79	0.41622887483799076
2226	80-81	0.5863820227498238
2226	82-83	0.37758811926254765
2226	84-85	0.348418301570419
2226	86-87	0.46302749829903433
2226	88-89	0.43751031041912825
2226	90-91	0.5430755709722419
2226	92-93	0.41397407234174466
2226	94-95	0.5839378651031026
2226	96-97	0.6556293350602758
2226	98-99	0.6016076341831038
2226	100	0.7976259226334363
2227	1	1.440202513031089
2227	2	1.7335692709153907
2227	3	0.7281255844040668
2227	4	-0.03916201912057282
2227	5	3.182850516345894
2227	6	-0.010644647532878082
2227	7	0.3045315815747145
2227	8	0.5620314200463241
2227	9	0.31405948624971813
2227	10-11	0.2814685643869552
2227	12-13	0.24952099910571945
2227	14-15	0.3038186092797943
2227	16-17	0.38299238443078565
2227	18-19	0.1750095026774261
2227	20-21	0.2813516723924465
2227	22-23	0.2726047265967608
2227	24-25	0.23351870375304173
2227	26-27	0.2855989948124602
2227	28-29	0.2716302757207103
2227	30-31	0.24966236508953443
2227	32-33	0.19588089769628425
2227	34-35	0.1547371821561896
2227	36-37	0.16064117581777282
2227	38-39	0.1540341539794099
2227	40-41	0.2367772732987632
2227	42-43	0.356463983374006
2227	44-45	0.1858928492676668
2227	46-47	0.2693582428526824
2227	48-49	0.1485979104170454
2227	50-51	0.22598790691659332
2227	52-53	0.2505975736172914
2227	54-55	0.259075639085772
2227	56-57	0.4839594403482792
2227	58-59	0.5928305583396423
2227	60-61	0.33313350008879183
2227	62-63	0.49903015690648544
2227	64-65	0.3356099949383733
2227	66-67	0.27591390155755846
2227	68-69	0.3351608795409078
2227	70-71	0.42782577929002485
2227	72-73	0.49059711928752847
2227	74-75	0.30438783821936255
2227	76-77	0.637282443290907
2227	78-79	0.5091945507222491
2227	80-81	0.20204851482029795
2227	82-83	0.38318708608791496
2227	84-85	0.23304104812469717
2227	86-87	0.223797773196317
2227	88-89	0.2559713775075352
2227	90-91	0.17762409716302585
2227	92-93	0.14824181797207814
2227	94-95	0.5083185241935126
2227	96-97	0.4702191393223245
2227	98-99	0.5730068935420007
2227	100	0.6288245288531371
2228	1	1.296889131316492
2228	2	1.8387264534981345
2228	3	-0.15962197392474486
2228	4	-0.07760053648356902
2228	5	3.2179783649622813
2228	6	0.05658948160439792
2228	7	0.18919415618133684
2228	8	0.41300707115864554
2228	9	0.09083664984169104
2228	10-11	0.16185372106085794
2228	12-13	0.09260326985992151
2228	14-15	0.05439952355000344
2228	16-17	0.09268090179377708
2228	18-19	0.15916704133721993
2228	20-21	0.0945233015297191
2228	22-23	0.15932288313501175
2228	24-25	0.2084219694882492
2228	26-27	0.15147223138326638
2228	28-29	0.21672531438050413
2228	30-31	0.19366823188281046
2228	32-33	0.10444243120143426
2228	34-35	0.12782437016487336
2228	36-37	0.29022702429851677
2228	38-39	0.17262001941620042
2228	40-41	0.09922674427000544
2228	42-43	0.3657418252079694
2228	44-45	0.24223585320147123
2228	46-47	0.2661115831945082
2228	48-49	0.20423715349897265
2228	50-51	0.11637458963498659
2228	52-53	0.23394486743172394
2228	54-55	0.08246142313353033
2228	56-57	0.35976680266515615
2228	58-59	0.4922621067661268
2228	60-61	0.32725746576974757
2228	62-63	0.38744629228359884
2228	64-65	0.28413965106968675
2228	66-67	0.33583971883916064
2228	68-69	0.30582708274220494
2228	70-71	0.3428809712325034
2228	72-73	0.35996686291206004
2228	74-75	0.36537501944562223
2228	76-77	0.34203870007332426
2228	78-79	0.40925118392897986
2228	80-81	0.3479079322104255
2228	82-83	0.2905015869018115
2228	84-85	0.24028747039816523
2228	86-87	0.3473114058604452
2228	88-89	0.3780722320924568
2228	90-91	0.4629214214983506
2228	92-93	0.41914582977349113
2228	94-95	0.6426960676010012
2228	96-97	0.371705421877941
2228	98-99	0.4521895271556744
2228	100	0.6029830000956764
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	19.0
13	39.0
14	150.0
15	453.0
16	1833.0
17	4573.0
18	7527.0
19	9658.0
20	11219.0
21	12989.0
22	14864.0
23	16910.0
24	19252.0
25	21715.0
26	24393.0
27	27838.0
28	31324.0
29	35386.0
30	40385.0
31	46671.0
32	54486.0
33	64846.0
34	80533.0
35	103344.0
36	145669.0
37	240666.0
38	592979.0
39	1090113.0
40	1212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.704506205194278	18.514470433705803	18.979353607323862	34.80166975377605
2	20.180253496752705	51.97742351241066	10.904736745255128	16.937586245581503
3	40.43850403195501	15.564557003265769	23.930979357264054	20.06595960751516
4	17.677774581486364	16.411154637442444	22.03352996078928	43.877540820281915
5	17.19203701379502	35.77586765428369	25.997474312738728	21.034621019182563
6	17.74115741044121	27.830393177164265	30.684323523433694	23.744125888960838
7	20.280839244929837	26.622713340419473	36.40614176650758	16.69030564814311
8	20.477985018402123	27.67604562230868	34.494845887539164	17.35112347175003
9	21.000819311918676	28.015062307320086	27.513404987029105	23.470713393732133
10-11	19.497235701563135	30.077188586499975	28.693799108271715	21.73177660366517
12-13	24.134011736930162	26.587134544493303	27.213965547434015	22.064888171142524
14-15	21.849379148159954	27.340620137302313	28.51355418843137	22.296446526106358
16-17	21.04498736971256	29.63424923742534	28.095734728051752	21.22502866481035
18-19	21.467638289892772	29.63400859000232	29.757608808732318	19.14074431137259
20-21	22.743273256629745	29.093070205738737	29.83480109750034	18.32885544013118
22-23	22.011612533954423	30.195364982541957	28.70871924849882	19.084303235004796
24-25	22.210165169284355	28.26446559426771	26.417292997863424	23.10807623858452
26-27	20.182469982936247	26.374846494711125	29.955439501793197	23.487244020559437
28-29	19.678501792734394	28.019284413068068	30.59136633035812	21.71084746383941
30-31	20.042773042393705	30.128298762592028	28.160949863677587	21.66797833133668
32-33	20.703638255831905	30.13449969585868	28.590043046270573	20.571819002038836
34-35	21.41417753930235	29.05726927372978	30.034279299841877	19.494273887125992
36-37	22.12532769700046	28.011508129995516	28.679249194849255	21.183914978154768
38-39	21.656631295585626	29.263545622141486	28.86667842761436	20.213144654658528
40-41	20.900478802817098	29.693401443720536	29.80261994829707	19.603499805165296
42-43	20.590256843232314	29.918203865208444	29.227619167325823	20.263920124233422
44-45	20.669725480526626	29.874267274875262	28.67911961546763	20.77688762913048
46-47	20.003980014772335	27.905457063045287	28.834077812065555	23.256485110116827
48-49	20.878996181850965	28.145382142554347	30.29464130020692	20.680980375387765
50-51	22.098060492838517	30.57062687172789	28.06015593212558	19.271156703308012
52-53	20.504885209606776	30.21354974620605	28.227126149107185	21.05443889507999
54-55	22.470529401537956	28.253985652078363	28.018402310212753	21.257082636170928
56-57	21.628407573432447	28.74363065844593	29.30642283634002	20.321538931781603
58-59	21.302243481506498	27.99088284781591	28.916798488053132	21.79007518262446
60-61	19.53995136318763	30.43617820613945	29.50031663723665	20.52355379343627
62-63	20.44705461114743	31.216639701650095	27.063471742643586	21.27283394455889
64-65	20.010366358205804	30.149553228472687	28.83010094241303	21.009979470908483
66-67	20.426745628639562	30.011769510119596	29.455355645421943	20.106129215818903
68-69	21.01246294492469	29.819529241808823	29.249879768845194	19.918128044421294
70-71	19.546975672766894	30.76503296684582	29.498264932080044	20.189726428307246
72-73	20.256793013968288	30.62747519758079	28.468516097646578	20.647215690804344
74-75	20.039543925003898	29.017007108724872	30.543729894370593	20.399719071900638
76-77	19.69162328534083	30.25302780736507	28.686949912385828	21.368398994908272
78-79	19.586571429523442	29.359966709205725	28.404337281061753	22.649124580209083
80-81	20.712457981517144	29.48780721228666	28.287086083582142	21.512648722614056
82-83	20.170178453022107	28.293509887091933	30.09064633084874	21.445665329037222
84-85	20.347128354301127	29.14895594189957	28.42551425428732	22.078401449511986
86-87	22.024496426755995	28.37349738823501	28.96065858905824	20.641347595950755
88-89	21.920740364754852	27.646927284123528	29.665052107571622	20.76728024355
90-91	21.696197807738997	27.458463329220113	29.650890932294033	21.194447930746854
92-93	20.76615105179584	27.609738001597155	30.32931304046172	21.294797906145284
94-95	21.07049599655245	28.203729886966055	29.222408939940696	21.5033651765408
96-97	20.83338423951897	28.057582855833314	29.73019351384852	21.378839390799197
98-99	21.878312372942787	28.147011140494776	29.283163158582582	20.691513327979855
100	22.177955437280435	29.629732470408698	27.70466415430757	20.4876479380033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	6.0
2	16.5
3	21.5
4	25.0
5	33.5
6	30.5
7	33.0
8	55.0
9	66.0
10	76.0
11	84.0
12	98.0
13	125.0
14	149.5
15	181.0
16	227.5
17	309.5
18	455.0
19	735.0
20	1173.5
21	1847.5
22	2839.5
23	4417.5
24	6892.0
25	10293.5
26	14515.5
27	19393.5
28	25098.5
29	31720.5
30	39295.0
31	47862.5
32	57431.5
33	67842.0
34	78997.0
35	91577.0
36	104188.5
37	112917.0
38	118781.0
39	124319.0
40	127550.0
41	128257.0
42	126890.0
43	125169.0
44	125521.5
45	123976.0
46	115280.0
47	104686.0
48	98629.5
49	94800.0
50	90839.0
51	84957.0
52	76566.5
53	65946.0
54	54343.0
55	43551.0
56	33258.5
57	24001.5
58	16827.5
59	12178.0
60	9433.5
61	7802.0
62	6804.0
63	5570.0
64	4409.5
65	4275.0
66	4816.0
67	4186.0
68	2659.0
69	2457.5
70	3400.0
71	3467.5
72	2045.5
73	778.0
74	343.5
75	202.5
76	136.0
77	95.0
78	80.5
79	63.5
80	50.5
81	41.5
82	29.5
83	28.5
84	25.0
85	17.5
86	14.0
87	12.5
88	16.5
89	18.0
90	13.0
91	13.5
92	14.5
93	13.5
94	12.5
95	16.0
96	19.5
97	25.0
98	45.5
99	97.0
100	104.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.35418969014608
2	0.006812173205427378
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.036247425535357E-4
30-31	0.010384861870230322
32-33	0.0
34-35	0.0
36-37	0.0
38-39	6.478969081248864E-4
40-41	0.0014623958783390293
42-43	9.255670116069806E-5
44-45	0.0
46-47	0.0019251793841425194
48-49	0.0
50-51	0.0
52-53	0.0013698391771783313
54-55	0.019122214459800217
56-57	0.011717678366944375
58-59	0.01566059383639011
60-61	2.4064742301781492E-4
62-63	0.012050882491122886
64-65	7.404536092855844E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.2957938162497728E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2701047.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.33448194271259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.81867864002496	62.93468339074365
2	8.838700621397958	12.96363062233902
3	2.383157338156999	5.243028263451524
4	1.0421564463020994	3.057040123712913
5	0.5407262779660718	1.982694073372654
6	0.33802849403321134	1.487348669508052
7	0.2202920675416594	1.130850325447965
8	0.14628677286041186	0.8582291762231659
9	0.10912632501439096	0.7202450260118195
>10	0.5240282861544314	6.542084287728386
>50	0.027182351247494456	1.3646457548292423
>100	0.01107019536512204	1.4254408404530259
>500	5.147126683332154E-4	0.2507101732639723
>1k	5.147126682967755E-5	0.039369272914687795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.702268046427922E-5	0.0	0.0	0.0	0.0
9	3.702268046427922E-5	0.0	0.0	0.0	0.0
10-11	3.702268046427922E-5	0.0	0.0	0.0	0.0
12-13	3.702268046427922E-5	0.0	0.0	0.0	0.0
14-15	3.702268046427922E-5	0.0	0.0	0.0	0.0
16-17	3.702268046427922E-5	0.0	0.0	0.0	0.0
18-19	3.702268046427922E-5	0.0	0.0	0.0	0.0
20-21	3.702268046427922E-5	0.0	0.0	0.0	0.0
22-23	3.702268046427922E-5	0.0	0.0	0.0	0.0
24-25	3.702268046427922E-5	0.0	0.0	0.0	0.0
26-27	3.702268046427922E-5	0.0	0.0	0.0	0.0
28-29	3.702268046427922E-5	0.0	0.0	0.0	0.0
30-31	3.702268046427922E-5	0.0	0.0	0.0	0.0
32-33	3.702268046427922E-5	0.0	0.0	3.702268046427922E-5	0.0
34-35	3.702268046427922E-5	0.0	0.0	3.702268046427922E-5	0.0
36-37	3.702268046427922E-5	0.0	0.0	3.702268046427922E-5	0.0
38-39	3.702268046427922E-5	0.0	0.0	3.702268046427922E-5	0.0
40-41	3.702268046427922E-5	0.0	0.0	3.702268046427922E-5	0.0
42-43	3.702268046427922E-5	0.0	0.0	3.702268046427922E-5	0.0
44-45	7.404536092855844E-5	0.0	0.0	3.702268046427922E-5	0.0
46-47	7.404536092855844E-5	0.0	0.0	3.702268046427922E-5	0.0
48-49	7.404536092855844E-5	0.0	0.0	3.702268046427922E-5	0.0
50-51	1.1106804139283767E-4	0.0	0.0	3.702268046427922E-5	0.0
52-53	1.2957938162497728E-4	0.0	0.0	3.702268046427922E-5	0.0
54-55	1.480907218571169E-4	0.0	0.0	3.702268046427922E-5	0.0
56-57	1.480907218571169E-4	0.0	0.0	3.702268046427922E-5	0.0
58-59	1.666020620892565E-4	0.0	0.0	3.702268046427922E-5	0.0
60-61	1.851134023213961E-4	0.0	0.0	3.702268046427922E-5	0.0
62-63	2.2213608278567534E-4	0.0	0.0	3.702268046427922E-5	0.0
64-65	2.7767010348209414E-4	0.0	0.0	3.702268046427922E-5	0.0
66-67	3.5171546441065257E-4	0.0	0.0	3.702268046427922E-5	0.0
68-69	4.442721655713507E-4	0.0	0.0	3.702268046427922E-5	0.0
70-71	5.183175264999091E-4	0.0	0.0	3.702268046427922E-5	0.0
72-73	5.73851547196328E-4	0.0	0.0	3.702268046427922E-5	0.0
74-75	6.478969081248864E-4	0.0	0.0	3.702268046427922E-5	0.0
76-77	7.959876299820033E-4	0.0	0.0	3.702268046427922E-5	0.0
78-79	9.811010323033994E-4	0.0	0.0	3.702268046427922E-5	0.0
80-81	0.0011106804139283766	0.0	0.0	3.702268046427922E-5	0.0
82-83	0.0012217484553212143	0.0	0.0	3.702268046427922E-5	0.0
84-85	0.0013698391771783313	0.0	0.0	3.702268046427922E-5	0.0
86-87	0.0015179298990354482	1.851134023213961E-4	0.0	3.702268046427922E-5	0.0
88	0.0015179298990354482	1.851134023213961E-4	0.0	3.702268046427922E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10825	0.0	79.60937	8
CACCGGC	11025	0.0	75.270195	9
CTTCACC	12065	0.0	74.92873	6
CCTTCAC	13340	0.0	71.70802	5
GGCGCGC	495	0.0	70.1691	6
GTGTGGC	4835	0.0	69.60526	7
CAGCTGC	85	0.0	68.539185	1
CGTAGTG	2145	0.0	68.05382	4
CGGGGCA	1130	0.0	67.29084	94
GCTGCCT	145	0.0	65.746056	1
TTCACCG	13465	0.0	65.29063	7
CGGGCCC	590	0.0	65.23509	7
CGCTGCC	65	2.5465852E-10	65.18412	1
TGTGGCA	5285	0.0	63.05692	8
ACCTTCA	5280	0.0	61.60539	4
GCGTAGT	1495	0.0	60.594822	3
AGGCGCG	575	0.0	60.406445	5
GCGCGCA	595	0.0	59.953705	7
TGTGTGG	5240	0.0	59.925858	6
GCCTTCA	3945	0.0	57.82407	4
>>END_MODULE