FastQCFastQC Report
Tue 7 Mar 2017
1NF_30A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_30A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2701047
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG108250.079.609378
CACCGGC110250.075.2701959
CTTCACC120650.074.928736
CCTTCAC133400.071.708025
GGCGCGC4950.070.16916
GTGTGGC48350.069.605267
CAGCTGC850.068.5391851
CGTAGTG21450.068.053824
CGGGGCA11300.067.2908494
GCTGCCT1450.065.7460561
TTCACCG134650.065.290637
CGGGCCC5900.065.235097
CGCTGCC652.5465852E-1065.184121
TGTGGCA52850.063.056928
ACCTTCA52800.061.605394
GCGTAGT14950.060.5948223
AGGCGCG5750.060.4064455
GCGCGCA5950.059.9537057
TGTGTGG52400.059.9258586
GCCTTCA39450.057.824074
Produced by FastQC (version 0.11.5)