FastQCFastQC Report
Tue 7 Mar 2017
1NF_31A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_31A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3561574
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA190350.5344547101927406No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG82100.23051605834948255No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA78240.2196781535354874No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG77900.21872351943270024No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT76560.21496113796877447No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG75290.2113952988201284No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA64890.1821947262642865No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG64110.1800046833225984No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA63630.17865696458925184No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA59190.16619056630579626No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG58410.16400052336410811No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC51960.14589055288476388No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA51060.14336358025973908No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA50620.14212817142083808No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC50410.14153854447499897No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT49920.14016274826804104No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT49050.13772000806385043No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC45290.12716287798596912No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA43890.12323203168037503No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA42210.11851501611366211No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG40780.11449993738723385No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA40220.11292759886499622No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA37520.10534668098992188No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT36880.10354972267879313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT600.088.999713
GCTGCTC355.5479177E-1088.999713
GCTGCAG355.5479177E-1088.999713
GCTGCTA650.088.99973
GCTGCCT256.8168447E-788.99973
GCTGCAA800.088.99973
CAGCTGC6750.088.340451
CAGCAGC6000.087.516381
AGCTGCC2700.087.351562
AGCTGCT1850.086.594312
AGCAGCG1550.086.128752
AGCTGCA1350.085.703422
CTGCTGC2350.085.212491
GCTGCCC2300.085.130153
GCAGCAA1150.085.130153
AGCTGCG1000.084.549722
AGCAGCC1000.084.549722
TGCTGCT950.084.3155062
AGCAGCA2600.083.8651052
CAGCCCT196800.083.3921
Produced by FastQC (version 0.11.5)