FastQCFastQC Report
Tue 7 Mar 2017
1NF_31A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_31A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3561574
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG141300.080.85548
CACCGGC142100.076.898799
CTTCACC158850.074.374936
CCTTCAC175750.069.7603155
CGGGGCA14500.069.5986994
GGCGCGC6550.068.078966
GTGTGGC58150.067.9661947
TTCACCG176200.067.078057
CGTAGTG28150.066.697914
CGGGCCC10700.065.801857
GGCCCGA10850.063.5943079
TGTGGCA64150.060.9507338
ACCTTCA71250.060.476744
GGGCCCG11550.060.1464968
AGGCTGC551.3000317E-859.7442972
AGGCGCG7550.059.6835865
TGTGTGG61950.058.6448976
AACGGGC12000.057.4998475
GCGCGCA7900.057.039377
ATGTGTG59700.056.9239235
Produced by FastQC (version 0.11.5)