FastQCFastQC Report
Tue 7 Mar 2017
1NF_32A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_32A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3477986
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA168420.4842457675217784No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG102850.29571711904533254No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA93440.26866123095377614No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC77290.22222631143426108No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG72840.20943155032826466No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA68640.19735559602597594No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG67770.1948541483490733No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT66820.19212268249498415No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG66790.19203642567853926No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA66730.19186391204564943No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA63810.1834682485783439No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG56790.16328415353023273No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA55480.15951760587880456No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG53890.15494599460722383No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA52630.15132320831653723No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT51390.1477579265701472No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG48330.1389597312927654No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT45940.13208793824932014No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC45570.13102410417983282No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC44370.12757383152203602No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA43890.1261937224589173No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41720.1199544794027348No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA40120.11535411585900576No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA39600.11385899770729381No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38240.10994868869512414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCT301.9390427E-888.99993
GCTGCAC301.9390427E-888.99993
GCAGCTC256.8167355E-788.99993
GCAGCTA256.8167355E-788.99993
TGCAGCG301.9390427E-888.99992
AGCTGCG450.088.999892
CAGCAGC3200.087.6092761
AGCTGCC2350.087.1062852
CAGCTGC5050.086.356331
AGCTGCA1350.085.7036062
AGCAGCG1150.085.130342
CTGCAGC1800.084.055461
CTGCTGC2650.083.9621661
CAGCCCT179400.082.3771
GCTGCCC2000.082.3249053
AGCAGCC650.082.1537552
TGCTGCC600.081.5832442
GCTGCAT501.8189894E-1280.099913
CAGCTAG54850.080.075581
AGCTGCT950.079.6314852
Produced by FastQC (version 0.11.5)