##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_32A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3477986 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.825723277782027 12.0 12.0 12.0 2.0 32.0 2 14.707753855248411 12.0 12.0 12.0 12.0 22.0 3 13.67521232115368 12.0 12.0 12.0 12.0 22.0 4 13.77303186384304 12.0 12.0 12.0 12.0 22.0 5 22.445645842162676 22.0 22.0 22.0 22.0 27.0 6 26.900235366099807 27.0 22.0 32.0 22.0 32.0 7 35.0060253261514 37.0 37.0 37.0 27.0 41.0 8 37.63365838735406 41.0 37.0 41.0 32.0 41.0 9 38.562367128562336 41.0 41.0 41.0 32.0 41.0 10-11 38.902742132947054 41.0 41.0 41.0 37.0 41.0 12-13 39.08619816180973 41.0 41.0 41.0 37.0 41.0 14-15 39.04261589897142 41.0 41.0 41.0 37.0 41.0 16-17 38.951497217067576 41.0 41.0 41.0 37.0 41.0 18-19 38.90057579300205 41.0 41.0 41.0 37.0 41.0 20-21 38.90675120601405 41.0 41.0 41.0 37.0 41.0 22-23 38.88819147058096 41.0 41.0 41.0 37.0 41.0 24-25 38.91159179479158 41.0 41.0 41.0 37.0 41.0 26-27 38.87977654309132 41.0 41.0 41.0 37.0 41.0 28-29 38.82162104735326 41.0 41.0 41.0 37.0 41.0 30-31 38.70380343681659 41.0 41.0 41.0 32.0 41.0 32-33 38.74524336785714 41.0 41.0 41.0 32.0 41.0 34-35 38.7212865434191 41.0 41.0 41.0 32.0 41.0 36-37 38.649633581043744 41.0 41.0 41.0 32.0 41.0 38-39 38.5603597886823 41.0 41.0 41.0 32.0 41.0 40-41 38.59811410971752 41.0 41.0 41.0 32.0 41.0 42-43 38.34167403204038 41.0 41.0 41.0 32.0 41.0 44-45 38.444728932203866 41.0 41.0 41.0 32.0 41.0 46-47 38.46184803504097 41.0 41.0 41.0 32.0 41.0 48-49 38.40417227096371 41.0 41.0 41.0 32.0 41.0 50-51 38.31335405030383 41.0 41.0 41.0 32.0 41.0 52-53 38.27488207255578 41.0 41.0 41.0 32.0 41.0 54-55 38.15363647237223 41.0 39.0 41.0 32.0 41.0 56-57 37.948173023123154 41.0 37.0 41.0 29.5 41.0 58-59 37.937460932850215 41.0 37.0 41.0 29.5 41.0 60-61 37.95132211573019 41.0 37.0 41.0 32.0 41.0 62-63 37.92784717937335 41.0 37.0 41.0 32.0 41.0 64-65 37.90942200457391 41.0 37.0 41.0 29.5 41.0 66-67 37.785205144586556 41.0 37.0 41.0 27.0 41.0 68-69 37.806454223795036 41.0 37.0 41.0 27.0 41.0 70-71 37.75980826259796 41.0 37.0 41.0 27.0 41.0 72-73 37.61125605450971 41.0 37.0 41.0 27.0 41.0 74-75 37.54048204909393 41.0 37.0 41.0 27.0 41.0 76-77 36.48803316057051 39.0 34.5 41.0 24.5 41.0 78-79 37.00184028917886 41.0 37.0 41.0 27.0 41.0 80-81 37.6449051261276 41.0 37.0 41.0 27.0 41.0 82-83 37.810368558125305 41.0 37.0 41.0 27.0 41.0 84-85 37.72498279176512 41.0 37.0 41.0 27.0 41.0 86-87 37.59138205271672 41.0 37.0 41.0 27.0 41.0 88-89 37.51458602766083 41.0 37.0 41.0 27.0 41.0 90-91 37.3634407959089 41.0 37.0 41.0 27.0 41.0 92-93 37.26520707673924 41.0 37.0 41.0 27.0 41.0 94-95 37.104594871859746 41.0 37.0 41.0 27.0 41.0 96-97 37.03899555662386 41.0 37.0 41.0 27.0 41.0 98-99 36.92250285078778 41.0 37.0 41.0 27.0 41.0 100 36.76658014149568 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3402388145951516 1101 2 -0.5419735178090761 1101 3 -0.023012153423501047 1101 4 -0.011452865030761927 1101 5 -1.0767266168469227 1101 6 -0.7335679275508333 1101 7 -0.30416062188847803 1101 8 -0.11911647228600941 1101 9 -0.025905578649286554 1101 10-11 -0.007025513317522325 1101 12-13 0.0247101063941102 1101 14-15 0.04090912426332949 1101 16-17 0.032954260192227025 1101 18-19 0.09332699217750928 1101 20-21 0.0724981810842209 1101 22-23 0.14126573138879905 1101 24-25 0.01735306166986561 1101 26-27 0.11158659059759657 1101 28-29 -0.027948825159548107 1101 30-31 0.1184433850169242 1101 32-33 -0.12137495960296718 1101 34-35 -0.0415715128959917 1101 36-37 -0.053213611749676204 1101 38-39 0.014171944703107897 1101 40-41 -0.006455497392835241 1101 42-43 0.07993188029850984 1101 44-45 0.03968354856437628 1101 46-47 0.09440148239288959 1101 48-49 0.09668130008645193 1101 50-51 0.0911611202917939 1101 52-53 0.1351042770580051 1101 54-55 -0.026906420177212453 1101 56-57 0.1777399623689817 1101 58-59 0.21226985552031863 1101 60-61 -0.05717766847802608 1101 62-63 -0.06332379804847932 1101 64-65 -0.09376442285996234 1101 66-67 -0.13469010624861255 1101 68-69 -0.04430496254783378 1101 70-71 0.011260160862050839 1101 72-73 0.10770663211642528 1101 74-75 0.03599932576877052 1101 76-77 -0.3132446788164387 1101 78-79 0.042068165452725736 1101 80-81 0.0237780878406042 1101 82-83 0.04286788117617846 1101 84-85 0.015925837814904753 1101 86-87 0.03020124080074993 1101 88-89 -0.19174009544344273 1101 90-91 -0.2660639558407709 1101 92-93 -0.051860243721371546 1101 94-95 -0.03134721593701073 1101 96-97 -0.1276671725665608 1101 98-99 -0.16374561227652862 1101 100 -0.3979529350763542 1102 1 1.6236422492149778 1102 2 0.729199516105103 1102 3 -0.16026899226733526 1102 4 0.033297303679953316 1102 5 -0.6994576025164854 1102 6 -0.044450723262215774 1102 7 0.03127499926083033 1102 8 -0.27348055623079404 1102 9 -0.24292333092061114 1102 10-11 -0.22776777182308905 1102 12-13 -0.17423441663184747 1102 14-15 -0.11963108036892578 1102 16-17 -0.27456097096397514 1102 18-19 -0.09298846661481974 1102 20-21 -0.09239943628716674 1102 22-23 -0.13324344162624158 1102 24-25 -0.14340433253575213 1102 26-27 -0.13880172290874526 1102 28-29 -0.2734312820337408 1102 30-31 -0.2174232904016833 1102 32-33 -0.2990285228204712 1102 34-35 -0.10542186895818162 1102 36-37 -0.24024402644126042 1102 38-39 -0.19030528160263316 1102 40-41 -0.033352468665469814 1102 42-43 -0.11471046585512568 1102 44-45 -0.06485225993640853 1102 46-47 -0.32114968789959875 1102 48-49 -0.33096191310940526 1102 50-51 -0.15249217135830406 1102 52-53 -0.0788469193186927 1102 54-55 -0.2137471023476749 1102 56-57 0.14608165937783468 1102 58-59 0.0410210787534524 1102 60-61 -0.007287239277104618 1102 62-63 -0.15127409906786937 1102 64-65 -0.09779873259181926 1102 66-67 -0.09572677943204155 1102 68-69 -0.2785270785169871 1102 70-71 -0.295006149806774 1102 72-73 -0.042717364832405735 1102 74-75 -0.2190961561303908 1102 76-77 0.06445560429150277 1102 78-79 -0.1253937368147504 1102 80-81 -0.09761533181941218 1102 82-83 -0.1594373421418993 1102 84-85 -0.2439660478968264 1102 86-87 -0.20410898442001013 1102 88-89 -0.24596750547546975 1102 90-91 -0.2567119336932251 1102 92-93 -0.025902657573801946 1102 94-95 0.0018407988022843824 1102 96-97 -0.10456957089716923 1102 98-99 -0.24470868339930973 1102 100 -0.3915171521878449 1103 1 1.4845681559754773 1103 2 0.7232022122381689 1103 3 0.31868272678286047 1103 4 1.0459327737518151 1103 5 2.935832351203434 1103 6 -0.0018532803427504518 1103 7 -0.4003696684460749 1103 8 0.12054396637319797 1103 9 -0.06081194825016212 1103 10-11 -0.15756183343079755 1103 12-13 -0.18956734660668673 1103 14-15 -0.14581587959192888 1103 16-17 -0.21241175353252117 1103 18-19 -0.29567660548689645 1103 20-21 -0.14490072331918924 1103 22-23 0.06079471422935967 1103 24-25 -0.03304249741384524 1103 26-27 -0.09888532028266894 1103 28-29 -0.15701525490257495 1103 30-31 -0.05023700114056595 1103 32-33 -0.25512663313901385 1103 34-35 -0.22930085743062278 1103 36-37 -0.15826041818727532 1103 38-39 -0.015014173534396491 1103 40-41 -0.21554279814566257 1103 42-43 -0.1327368752215321 1103 44-45 -0.31031132568384834 1103 46-47 -0.3420223287831661 1103 48-49 -0.2108642446276292 1103 50-51 -0.1384462734080003 1103 52-53 -0.12563899199752626 1103 54-55 -0.051029434715069044 1103 56-57 -0.3467812836530797 1103 58-59 -0.40751721111269745 1103 60-61 -0.21314071399820023 1103 62-63 -0.08533145425686683 1103 64-65 -0.21714740150879663 1103 66-67 -0.3505876472896716 1103 68-69 -0.2183895120424424 1103 70-71 -0.2682446739923563 1103 72-73 -0.2163252141355585 1103 74-75 -0.22365417341111282 1103 76-77 -0.20574714969166052 1103 78-79 -0.13735965169549758 1103 80-81 -0.05900652719353161 1103 82-83 -0.3382339539305832 1103 84-85 -0.3535953030635355 1103 86-87 -0.2971222580987458 1103 88-89 -0.138050036072336 1103 90-91 -0.34997996010259413 1103 92-93 -0.230705608990327 1103 94-95 -0.20020415576681216 1103 96-97 -0.17313653371388682 1103 98-99 -0.15277628442468938 1103 100 0.07432375738554953 1104 1 1.8077150738669214 1104 2 -0.7147100827369837 1104 3 -0.09842529141887191 1104 4 0.06269620756801508 1104 5 -1.0683932870723325 1104 6 -0.4437861667333607 1104 7 -0.148397124253691 1104 8 0.14249520682415806 1104 9 0.09004872026203259 1104 10-11 0.0693037518035382 1104 12-13 0.054930397547657606 1104 14-15 -0.038404893922979966 1104 16-17 0.11605983399464037 1104 18-19 -0.07375784454666956 1104 20-21 0.04930044581112014 1104 22-23 0.1352569367392107 1104 24-25 0.0820251669494354 1104 26-27 0.02831686978949932 1104 28-29 0.021964569278821955 1104 30-31 -0.015093807331552966 1104 32-33 -0.11716019830847557 1104 34-35 -0.0881409525776462 1104 36-37 0.03345883645032899 1104 38-39 0.04924660997133401 1104 40-41 0.08474255048022172 1104 42-43 0.008512345534363419 1104 44-45 0.03244629373861585 1104 46-47 -0.19945758328341157 1104 48-49 -0.2627347166470386 1104 50-51 -0.07777881781171203 1104 52-53 -0.16546458439914602 1104 54-55 -0.10521980889312488 1104 56-57 -0.12345212389835325 1104 58-59 -0.05750860420745596 1104 60-61 -0.06978533289941424 1104 62-63 -0.05741343822116818 1104 64-65 -0.21961467765881793 1104 66-67 -0.4131765779730472 1104 68-69 -0.3603933228542999 1104 70-71 -0.24413757987728246 1104 72-73 -0.14113371599693636 1104 74-75 -0.19297870084578506 1104 76-77 -0.2468321348464002 1104 78-79 -0.12946182556643038 1104 80-81 -0.2064445200544185 1104 82-83 -0.17355105785445346 1104 84-85 -0.047055419853293756 1104 86-87 -0.12889644284211244 1104 88-89 -0.16503890256713305 1104 90-91 -0.1892507373039365 1104 92-93 -0.12775194363737086 1104 94-95 -0.2169941697315707 1104 96-97 -0.07139234766737701 1104 98-99 -0.267134044199139 1104 100 -0.2841431700857555 1105 1 1.5883988011367673 1105 2 0.3791161907327929 1105 3 -0.07715060655047168 1105 4 -0.3215403445277545 1105 5 -1.0330520573987165 1105 6 0.4752166121303709 1105 7 0.19180775090875812 1105 8 0.06945525669578245 1105 9 0.19438966465306606 1105 10-11 0.07914515581651216 1105 12-13 -0.07392218531636985 1105 14-15 0.07046100212850348 1105 16-17 -0.046208796543268704 1105 18-19 0.014047856878690368 1105 20-21 0.007558954100161941 1105 22-23 -0.038056092222262805 1105 24-25 0.11598304022056993 1105 26-27 0.08480419584636678 1105 28-29 0.04806539025871359 1105 30-31 -0.005028667807231102 1105 32-33 -0.12249894496697067 1105 34-35 0.06842091676292483 1105 36-37 -0.07754805259587272 1105 38-39 -0.059476539125796535 1105 40-41 0.1436910728220795 1105 42-43 0.18952118929459516 1105 44-45 0.06007173883228489 1105 46-47 -0.21215001695521352 1105 48-49 -0.2274905887136498 1105 50-51 -0.11925272502090678 1105 52-53 -0.19217797049215335 1105 54-55 -0.16483319815593234 1105 56-57 -0.15635117612620064 1105 58-59 0.008867197489450973 1105 60-61 -0.14276436991218588 1105 62-63 -0.16552634672998323 1105 64-65 -0.24866621871309746 1105 66-67 -0.1912596903004271 1105 68-69 -0.24123897440803432 1105 70-71 -0.3895820395837575 1105 72-73 -0.23794483779146702 1105 74-75 -0.41901707663691923 1105 76-77 -0.35514231098198223 1105 78-79 -0.2752695979320592 1105 80-81 -0.26754819386019335 1105 82-83 -0.11624470661875819 1105 84-85 -0.12808455037536248 1105 86-87 -0.11860075272240067 1105 88-89 -0.1571293371476017 1105 90-91 -0.32339393859721355 1105 92-93 -0.049939479958077015 1105 94-95 -0.13057377942272552 1105 96-97 -0.1737816950042088 1105 98-99 -0.2986566607687706 1105 100 -0.5097757049800435 1106 1 1.228381280782239 1106 2 -0.5866675874801324 1106 3 -0.10683214834167032 1106 4 -0.4408539588738343 1106 5 -1.1295995169473088 1106 6 -0.847681460630632 1106 7 -0.2535503857500032 1106 8 -0.16762399714323806 1106 9 -0.094308676066305 1106 10-11 -0.0697850719480897 1106 12-13 -0.11547685912859151 1106 14-15 -0.15193361809205186 1106 16-17 -0.040319896203172334 1106 18-19 -0.02170345714790045 1106 20-21 -0.060462276447090346 1106 22-23 -0.01710741655493564 1106 24-25 -0.2060127342821687 1106 26-27 -0.2707075015668039 1106 28-29 -0.12128017296915772 1106 30-31 -0.1907851348792704 1106 32-33 -0.21984770308301904 1106 34-35 -0.0759710238739899 1106 36-37 -0.30757742495493545 1106 38-39 -0.18294505401809857 1106 40-41 -0.12415546948442824 1106 42-43 -0.12348200559357991 1106 44-45 -0.18830556715555247 1106 46-47 -0.39122349938332945 1106 48-49 -0.36908184914857145 1106 50-51 -0.2087544255259033 1106 52-53 -0.2971006748104017 1106 54-55 -0.38166407520426304 1106 56-57 -0.24881072471941934 1106 58-59 -0.3756793345109628 1106 60-61 -0.18635861911346296 1106 62-63 -0.35291375268690217 1106 64-65 -0.49751966610050147 1106 66-67 -0.4907371714918085 1106 68-69 -0.48550621134506144 1106 70-71 -0.5776063619582814 1106 72-73 -0.4999931733577867 1106 74-75 -0.6655696892309635 1106 76-77 -0.6288669322331444 1106 78-79 -0.36332638449291466 1106 80-81 -0.3848072889854137 1106 82-83 -0.5347024001156058 1106 84-85 -0.458792578820173 1106 86-87 -0.48460250475785216 1106 88-89 -0.3768021187615389 1106 90-91 -0.6316372654033557 1106 92-93 -0.40222794744235557 1106 94-95 -0.4740124066514184 1106 96-97 -0.5275295049598867 1106 98-99 -0.7397630196268565 1106 100 -0.8473372838731663 1107 1 1.2876260933636523 1107 2 -0.5541808701526048 1107 3 -0.1559228539425188 1107 4 -0.2811178402639989 1107 5 -1.133632765339243 1107 6 -0.12335181200615608 1107 7 -0.23135684578695503 1107 8 -0.3450978074541382 1107 9 -0.06710197001363127 1107 10-11 -0.13418517806439212 1107 12-13 -0.4393711614916427 1107 14-15 -0.31243317300464213 1107 16-17 -0.3605654663140285 1107 18-19 -0.3130596421011447 1107 20-21 -0.3466316645892178 1107 22-23 -0.16579075577786284 1107 24-25 -0.23092586710681928 1107 26-27 -0.33676811013963714 1107 28-29 -0.22771710061213923 1107 30-31 -0.24948102359810065 1107 32-33 -0.14257757596107723 1107 34-35 -0.3485204265034767 1107 36-37 -0.1938594062208594 1107 38-39 -0.28004892881745036 1107 40-41 -0.3650113326690132 1107 42-43 -0.21051773383222638 1107 44-45 -0.34805717833051375 1107 46-47 -0.34100178471388887 1107 48-49 -0.22547338720276144 1107 50-51 -0.4119937188162268 1107 52-53 -0.36744624709746887 1107 54-55 -0.24663046307384207 1107 56-57 -0.05416420909576658 1107 58-59 -0.23887070966005552 1107 60-61 -0.049233717949810796 1107 62-63 -0.2148140116562658 1107 64-65 -0.3724796911373218 1107 66-67 -0.2505774892603796 1107 68-69 -0.5066735113588479 1107 70-71 -0.2444228419023986 1107 72-73 -0.32037978280732204 1107 74-75 -0.3259630760300496 1107 76-77 -0.3658744215152936 1107 78-79 -0.6565432316989117 1107 80-81 -0.482264850238046 1107 82-83 -0.39170006534978086 1107 84-85 -0.40532561218152097 1107 86-87 -0.2817594377066541 1107 88-89 -0.24558983331284168 1107 90-91 -0.16535385405450853 1107 92-93 -0.12753414581558076 1107 94-95 -0.27322845176743016 1107 96-97 -0.33876254733698374 1107 98-99 -0.35150270515369186 1107 100 -0.44124233258407486 1108 1 1.3762397902020087 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0.06464789836815754 2223 20-21 0.10156683709985259 2223 22-23 0.12154405299089888 2223 24-25 0.15833601190399804 2223 26-27 0.1402434656316558 2223 28-29 0.047202409240149734 2223 30-31 0.10301873391457406 2223 32-33 0.23305286501124556 2223 34-35 0.1251790331408955 2223 36-37 -0.005307102486959536 2223 38-39 0.11560851429189256 2223 40-41 0.16862572690359912 2223 42-43 0.3107752085872022 2223 44-45 0.2789976801246823 2223 46-47 0.2928339058743674 2223 48-49 0.14939251469886017 2223 50-51 0.25002483954708765 2223 52-53 0.1362884748973343 2223 54-55 0.14516887631766906 2223 56-57 0.24912595280233774 2223 58-59 0.32943197292768645 2223 60-61 0.27519382944473136 2223 62-63 0.24711860711298073 2223 64-65 0.08280095274214716 2223 66-67 0.33753296606302285 2223 68-69 0.3712415774019391 2223 70-71 0.33603252322092914 2223 72-73 0.4037795683904619 2223 74-75 0.1896957124928349 2223 76-77 0.2945620765296866 2223 78-79 0.3374723375518087 2223 80-81 0.2511526670463411 2223 82-83 0.0923005687676195 2223 84-85 0.241677922397173 2223 86-87 0.28664196068906733 2223 88-89 0.2251240598029156 2223 90-91 0.2618363279726381 2223 92-93 0.3898608519576996 2223 94-95 0.4628921473483487 2223 96-97 0.5883586431349883 2223 98-99 0.40384463577723295 2223 100 0.5530787706967573 2224 1 1.3082021864725046 2224 2 -0.8840035391033272 2224 3 -0.12286506984837864 2224 4 -0.30396726146893727 2224 5 -1.062496605433605 2224 6 0.5148934099425375 2224 7 0.22302597453981576 2224 8 0.17419928470663848 2224 9 0.2134780687386666 2224 10-11 0.24168933714123142 2224 12-13 0.08816381547092078 2224 14-15 0.11520161165467613 2224 16-17 0.2035883733657755 2224 18-19 0.16938266119633738 2224 20-21 0.14394634092759873 2224 22-23 0.27164173445021333 2224 24-25 0.22022351507916937 2224 26-27 0.2661088139083958 2224 28-29 0.24840019457636942 2224 30-31 0.11996760293674669 2224 32-33 0.16981723378869873 2224 34-35 0.19906473137746872 2224 36-37 0.06305474020251722 2224 38-39 0.133689910000939 2224 40-41 0.2620486463194993 2224 42-43 0.29049742564655645 2224 44-45 0.3817812919431063 2224 46-47 0.2973233292233388 2224 48-49 0.2111250285818116 2224 50-51 0.20641667643349848 2224 52-53 0.2630702322136358 2224 54-55 0.16212538638423268 2224 56-57 0.36784516920290145 2224 58-59 0.42300946860636657 2224 60-61 0.4123383918090724 2224 62-63 0.3212289933574439 2224 64-65 0.23020599211417192 2224 66-67 0.24087365132014327 2224 68-69 0.2854277958490812 2224 70-71 0.4893922078316635 2224 72-73 0.35783809879045947 2224 74-75 0.28041628362993265 2224 76-77 0.4361270863288169 2224 78-79 0.3026618044753846 2224 80-81 0.2930245577091952 2224 82-83 0.28012848831774306 2224 84-85 0.11438359556285604 2224 86-87 0.5075799711676297 2224 88-89 0.32451205345052614 2224 90-91 0.2729106794648146 2224 92-93 0.1703208428297529 2224 94-95 0.36838275402662646 2224 96-97 0.32903109634391114 2224 98-99 0.3682639060781412 2224 100 0.5549219145115387 2225 1 1.4814386713557735 2225 2 1.7310847437994905 2225 3 0.8325226531571044 2225 4 0.9750757824246801 2225 5 3.175905742979811 2225 6 0.12905991821801166 2225 7 0.22617330071013697 2225 8 0.3323811525798135 2225 9 0.29480604534398225 2225 10-11 0.1993976464053091 2225 12-13 0.19395733050069452 2225 14-15 0.11632184112935562 2225 16-17 0.10407988297670556 2225 18-19 0.08905534424376071 2225 20-21 0.14994222399887036 2225 22-23 0.09694432374246986 2225 24-25 0.07067148264064826 2225 26-27 0.040504784436450336 2225 28-29 0.014623490131846495 2225 30-31 0.027242603588952363 2225 32-33 0.06085154691131578 2225 34-35 0.045984923335169015 2225 36-37 0.08551895932151155 2225 38-39 0.22437352638864638 2225 40-41 0.012971973801668923 2225 42-43 0.3134476883274786 2225 44-45 0.07666488373359215 2225 46-47 0.13182161425046246 2225 48-49 0.0465652753490815 2225 50-51 0.03777198328382525 2225 52-53 0.21249840895087857 2225 54-55 0.16737869427559815 2225 56-57 0.2604466234203997 2225 58-59 0.38381603382812557 2225 60-61 0.2201853399524225 2225 62-63 0.36637731583943633 2225 64-65 0.20577368478308955 2225 66-67 0.34510685781629036 2225 68-69 0.4706768551296179 2225 70-71 0.2618467992507121 2225 72-73 0.2613306556466881 2225 74-75 0.31719161286621045 2225 76-77 0.454405119395922 2225 78-79 0.27615705708759464 2225 80-81 0.28813586013202297 2225 82-83 0.1931089881879302 2225 84-85 0.11781882101875141 2225 86-87 0.09966519432528997 2225 88-89 0.13857073148657406 2225 90-91 0.346307294738196 2225 92-93 0.21942087028422463 2225 94-95 0.41344163207038065 2225 96-97 0.34870421419068975 2225 98-99 0.2573574612336813 2225 100 0.2318411499106645 2226 1 1.5238930378064754 2226 2 1.6718021514260517 2226 3 0.9754967937314749 2226 4 0.8907409391607768 2226 5 3.0389667667160545 2226 6 -0.3546896819238654 2226 7 0.0438680378313947 2226 8 0.2662986931332938 2226 9 0.1635251651091565 2226 10-11 0.09798813616314561 2226 12-13 0.1115770268902807 2226 14-15 0.10229324360789604 2226 16-17 0.08607684074008404 2226 18-19 0.14050651845925444 2226 20-21 0.1282329320419464 2226 22-23 0.21086027729428736 2226 24-25 0.24424823633964365 2226 26-27 0.23454691912358783 2226 28-29 0.11460759939711807 2226 30-31 0.11551532423190736 2226 32-33 0.14694814849020332 2226 34-35 0.14648045735501825 2226 36-37 0.17920105595375446 2226 38-39 0.16337610205506792 2226 40-41 0.05228880066020736 2226 42-43 0.3559800581891963 2226 44-45 0.1668621028757329 2226 46-47 0.2831906000113733 2226 48-49 0.25217770882346 2226 50-51 0.25174466282219754 2226 52-53 0.3149996561767239 2226 54-55 0.3212297009235954 2226 56-57 0.3846001880001779 2226 58-59 0.2261061891139633 2226 60-61 0.4083988691623688 2226 62-63 0.35143733185032744 2226 64-65 0.38694929666960576 2226 66-67 0.2627422999143505 2226 68-69 0.40261623746690844 2226 70-71 0.5131793834198035 2226 72-73 0.43975936653725256 2226 74-75 0.5181025021009589 2226 76-77 0.5289504827820153 2226 78-79 0.400390075341015 2226 80-81 0.29443497223981296 2226 82-83 0.3164966069229962 2226 84-85 0.3287771296976203 2226 86-87 0.3824808046206627 2226 88-89 0.24376066202313496 2226 90-91 0.30288477439722783 2226 92-93 0.25784806461444276 2226 94-95 0.5050993692629007 2226 96-97 0.6004817182141338 2226 98-99 0.48557689922790814 2226 100 0.5104598037686117 2227 1 1.4195617483743792 2227 2 1.7638829281635378 2227 3 0.6727846702061164 2227 4 -0.21133996136615352 2227 5 3.1303197646900323 2227 6 0.23835128825525942 2227 7 0.26532765977843553 2227 8 0.4037958562919073 2227 9 0.29669691883418636 2227 10-11 0.2782379948368643 2227 12-13 0.15311374902837116 2227 14-15 0.12477959582187026 2227 16-17 0.12729557874798303 2227 18-19 0.14255482109967943 2227 20-21 0.13494154613661635 2227 22-23 0.24173032107448478 2227 24-25 0.2211440716501727 2227 26-27 0.3395792178522612 2227 28-29 0.19380349412657694 2227 30-31 0.1933793992978181 2227 32-33 0.18172230496831077 2227 34-35 0.1616878871973526 2227 36-37 0.31450781650783455 2227 38-39 0.10794618132156586 2227 40-41 0.21891423856536107 2227 42-43 0.3374826660201151 2227 44-45 0.3211598660005066 2227 46-47 0.25807470214591177 2227 48-49 0.33869229328293216 2227 50-51 0.3378676084332426 2227 52-53 0.3874515536625651 2227 54-55 0.42366108030935834 2227 56-57 0.5634083123012843 2227 58-59 0.5896648467412575 2227 60-61 0.42891299766405666 2227 62-63 0.46095477997224066 2227 64-65 0.4214924826797457 2227 66-67 0.31052770678961394 2227 68-69 0.3374036497311437 2227 70-71 0.2851344726101033 2227 72-73 0.4297580048533831 2227 74-75 0.37886258990893396 2227 76-77 0.4591019804279526 2227 78-79 0.5885959330574337 2227 80-81 0.2920792591507251 2227 82-83 0.25441780050080354 2227 84-85 0.25263517209137376 2227 86-87 0.48411425789221596 2227 88-89 0.3252019940800679 2227 90-91 0.27045812995763896 2227 92-93 0.23614994167502346 2227 94-95 0.38957663739495985 2227 96-97 0.2465665160453483 2227 98-99 0.5089415891147269 2227 100 0.462364304340106 2228 1 1.489201636374725 2228 2 1.3918871605412289 2228 3 -0.04676272450629071 2228 4 -0.16038227794512672 2228 5 3.1575495007422596 2228 6 0.17458922273977961 2228 7 0.2307639617744215 2228 8 0.4238938919792048 2228 9 0.34410386770734647 2228 10-11 0.13383941714609904 2228 12-13 0.16057007564125314 2228 14-15 0.2284890445330845 2228 16-17 0.03679899416161447 2228 18-19 0.09729753140494779 2228 20-21 0.02498430178878408 2228 22-23 0.1512405745132952 2228 24-25 0.1899353247362825 2228 26-27 0.21402119900811556 2228 28-29 0.271766698353197 2228 30-31 0.24614376095017576 2228 32-33 0.2209621325061093 2228 34-35 0.31535846713386917 2228 36-37 0.2668377836133331 2228 38-39 0.22288474706378736 2228 40-41 0.15669648705199535 2228 42-43 0.35627967744321865 2228 44-45 0.3048479368856434 2228 46-47 0.2662292271190978 2228 48-49 0.1923794284383007 2228 50-51 0.2641544168029881 2228 52-53 0.4677731567835792 2228 54-55 0.3453315018916001 2228 56-57 0.42916276241609097 2228 58-59 0.3709761640328182 2228 60-61 0.2863099414267012 2228 62-63 0.36542073020581256 2228 64-65 0.34515133000464004 2228 66-67 0.4254601542987757 2228 68-69 0.30248041556117045 2228 70-71 0.3967904263706501 2228 72-73 0.40852485698648167 2228 74-75 0.35433747276276506 2228 76-77 0.4933129802911864 2228 78-79 0.39154024274079546 2228 80-81 0.3786886063712487 2228 82-83 0.2200034593625233 2228 84-85 0.23489265612997912 2228 86-87 0.361434687752066 2228 88-89 0.40497115674588713 2228 90-91 0.47578783074005315 2228 92-93 0.5160803377766285 2228 94-95 0.6482199012203651 2228 96-97 0.5364246450176964 2228 98-99 0.6444291403099385 2228 100 0.6271398371662258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 22.0 13 39.0 14 181.0 15 596.0 16 2306.0 17 5571.0 18 9213.0 19 11787.0 20 14069.0 21 15914.0 22 18508.0 23 21506.0 24 24072.0 25 27243.0 26 30863.0 27 35042.0 28 39458.0 29 44790.0 30 51500.0 31 59219.0 32 69925.0 33 83307.0 34 102354.0 35 130842.0 36 184416.0 37 303394.0 38 745597.0 39 1444372.0 40 1880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.50561601062257 18.110305139602794 20.980422413211823 35.403656436562805 2 17.934844647969655 50.02871058835572 15.908800104433507 16.127644659241113 3 41.42621620673574 16.559813639272843 22.986003968963647 19.027966185027772 4 16.903029511907178 15.639855939615629 22.87168493490198 44.585429613575215 5 17.236699630188276 35.86475046190525 26.21433783804765 20.68421206985882 6 18.266951045806394 28.78536601354922 30.078643214780048 22.869039725864337 7 20.44775338371115 26.608646498289527 35.437089165971344 17.506510952027984 8 21.05572017828709 28.26302348543094 33.05438837304118 17.626867963240795 9 21.232977936081397 28.45028703393286 27.095940006659024 23.22079502332672 10-11 19.8241597292226 30.0009114470271 28.735710839549096 21.4392179842012 12-13 23.71639793834708 27.07881802859471 27.38525974515136 21.81952428790685 14-15 21.89604846022957 27.64852992507733 28.39955364972717 22.05586796496593 16-17 20.656365494283186 29.573422664726078 28.584200741463594 21.186011099527143 18-19 21.309746502717378 29.595389400647388 29.691407613486653 19.40345648314858 20-21 22.654605280182267 29.00225877849997 29.877204796109012 18.46593114520875 22-23 21.501984194300956 29.935744422202966 28.932290124227066 19.629981259269012 24-25 22.064047986392126 28.640785213051462 26.5441407757248 22.75102602483161 26-27 20.256737663693876 26.81311828167221 29.90667587506103 23.02346817957289 28-29 19.683218010017608 27.94377073674038 30.669710008020473 21.70330124522154 30-31 20.075271575196524 30.08673325906914 28.293983277328465 21.54401188840587 32-33 21.00550433498007 29.934048038146216 28.62833547921124 20.43211214766247 34-35 21.33589669423626 28.85826739958125 30.228916965163172 19.576918941019315 36-37 22.011028796550647 28.278391574894208 28.681153403147686 21.029426225407462 38-39 21.381781870025186 29.38587392575271 29.024411878405104 20.207932325816998 40-41 20.765450127740827 29.691077233782465 29.872908981622885 19.67056365685382 42-43 20.729282304877454 29.687827506820767 29.161286390006158 20.42160379829562 44-45 20.55281418614106 30.19612499877803 28.679255753185895 20.571805061895017 46-47 20.125661567727544 28.247170070096633 28.879560817076218 22.747607545099605 48-49 21.00957278148906 28.4211178538384 30.02341009998315 20.545899264689393 50-51 22.153280663004395 30.555902180169785 28.13842551407625 19.15239164274957 52-53 20.405392447278757 30.14146167334471 28.452084474754223 21.001061404622305 54-55 21.942441698451304 28.6668800500617 27.968552694051635 21.42212555743536 56-57 21.09315489584037 28.99227770916395 29.288157476646937 20.62640991834874 58-59 21.02386438387186 28.171389339209263 29.138531328519 21.666214948399876 60-61 19.767540153370767 30.080446190414715 29.66566483907168 20.486348817142837 62-63 20.389191526952168 31.058271182619578 27.277626203139665 21.274911087288586 64-65 20.05914632454515 30.18604597001511 28.782761970330807 20.972045735108942 66-67 20.068122758400982 30.027262904451025 29.564049424005734 20.340564913142263 68-69 20.87563032168617 29.855539959045263 29.035611414192008 20.23321830507656 70-71 19.440963822166047 30.69159852857372 29.646295298485963 20.221142350774272 72-73 20.216656996319134 30.509769159507833 28.562363390766954 20.711210453406082 74-75 20.13308851732008 29.139450245055613 30.303011570489357 20.424449667134944 76-77 19.658747907553394 30.183531503577072 28.904860456597582 21.25286013227195 78-79 19.63921073862862 29.390112553644553 28.423188017433077 22.54748869029375 80-81 20.791226175297368 29.577169976321038 28.466353256910654 21.165250591470944 82-83 20.312761465974848 28.476509106132113 29.835902157167972 21.37482727072507 84-85 20.263738841961988 29.365256214372344 28.467696534718655 21.903308408947016 86-87 21.801266595092677 28.351264208654086 29.231428763658045 20.616040432595188 88-89 20.99493787496557 28.649813426506032 29.658701903917954 20.696546794610445 90-91 20.446402026920175 29.10887220362589 30.36241376474776 20.08231200470617 92-93 20.16163952356335 29.10208666739889 31.296618215254462 19.439655593783296 94-95 20.29254861865459 30.156648129118402 29.77483233112497 19.77597092110204 96-97 20.210144606677545 29.56725530235027 30.284883262899854 19.93771682807234 98-99 21.316316396903265 29.497904821928557 29.84454221494854 19.341236566219646 100 21.622858746412437 30.95742766072089 28.483208385542667 18.936505207324007 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 12.0 2 21.0 3 30.0 4 41.5 5 43.0 6 43.0 7 49.0 8 62.5 9 75.0 10 93.0 11 120.0 12 136.0 13 154.5 14 209.0 15 256.5 16 328.0 17 464.0 18 668.5 19 1036.0 20 1711.5 21 2649.0 22 3857.5 23 5781.5 24 8937.5 25 13685.5 26 19847.0 27 26757.0 28 34660.5 29 43647.0 30 53655.0 31 65855.5 32 79344.0 33 93795.5 34 109526.5 35 125868.5 36 142213.5 37 154075.0 38 160832.5 39 165512.5 40 168392.0 41 169065.5 42 166971.5 43 163035.0 44 161127.0 45 157866.0 46 144429.0 47 127291.0 48 116330.0 49 112377.0 50 109970.0 51 102969.0 52 90987.5 53 76095.5 54 61747.5 55 49328.0 56 37991.0 57 27983.5 58 20153.0 59 14777.5 60 11651.0 61 9760.5 62 8017.0 63 6190.0 64 4790.0 65 4927.0 66 6682.0 67 6691.5 68 4463.0 69 3803.5 70 5173.0 71 5075.0 72 2811.0 73 1042.5 74 418.5 75 240.5 76 172.0 77 142.5 78 117.0 79 94.0 80 63.0 81 40.0 82 30.5 83 28.0 84 29.5 85 27.5 86 23.0 87 23.0 88 22.5 89 20.0 90 18.5 91 16.0 92 12.5 93 13.0 94 17.0 95 20.0 96 22.5 97 23.5 98 50.5 99 115.0 100 121.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.306370985967167 2 0.005031647625953641 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.018988575572185E-4 30-31 0.008970708910271635 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.906874840784293E-4 40-41 0.0013082283827479467 42-43 1.437613607415326E-4 44-45 0.0 46-47 0.002098915866826376 48-49 0.0 50-51 0.0 52-53 0.0013657329270445598 54-55 0.019465288244403512 56-57 0.01124213840998785 58-59 0.015051814469638464 60-61 2.5877044933475865E-4 62-63 0.012018449757992124 64-65 4.312840822245978E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2938522466737933E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3477986.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.25116459041571 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3157927042795 61.64165380838686 2 9.058174973324371 13.089273817728872 3 2.5504901562654654 5.528276521997138 4 1.082263933305859 3.127793183022092 5 0.5786261009029992 2.090320482632654 6 0.34251958423868917 1.4848463313762172 7 0.21746159866088358 1.0998297629859697 8 0.16292592799894814 0.941727043191858 9 0.11511458774633825 0.7485446723416683 >10 0.5336774946870155 6.612998056191671 >50 0.028140190646958427 1.3816725832984766 >100 0.013920025270079222 1.7421631015573535 >500 6.898311603050313E-4 0.3202437553764949 >1k 2.0289151270963646E-4 0.19065687991264338 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.875227214830652E-5 0.0 0.0 0.0 0.0 12-13 2.875227214830652E-5 0.0 0.0 0.0 0.0 14-15 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 0.0 16-17 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 0.0 18-19 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 0.0 20-21 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 0.0 22-23 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 1.437613607415326E-5 24-25 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 26-27 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 28-29 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 30-31 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 32-33 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 34-35 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 36-37 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 38-39 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 40-41 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 42-43 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 44-45 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 46-47 2.875227214830652E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 48-49 8.625681644491956E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 50-51 8.625681644491956E-5 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 52-53 1.2938522466737935E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 54-55 2.0126590503814564E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 56-57 2.8752272148306515E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 58-59 4.0253181007629123E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 60-61 4.600363543729043E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 62-63 4.887886265212109E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 64-65 5.175408986695174E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 66-67 5.894215790402837E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 68-69 7.331829397818163E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 70-71 9.056965726716554E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 72-73 9.488249808941152E-4 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 74-75 0.001106962477709801 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 76-77 0.001207595430228874 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 78-79 0.0012938522466737934 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 80-81 0.0014376136074153258 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 82-83 0.0015669988320827053 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 84-85 0.0016820079206759314 0.0 0.0 2.875227214830652E-5 2.875227214830652E-5 86-87 0.001725136328898391 2.875227214830652E-5 0.0 2.875227214830652E-5 2.875227214830652E-5 88 0.001725136328898391 2.875227214830652E-5 0.0 2.875227214830652E-5 2.875227214830652E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCTGC 45 9.094947E-12 94.03591 1 TCACCGG 12240 0.0 79.22567 8 GGCGCGC 840 0.0 74.31721 6 CACCGGC 12955 0.0 72.1358 9 CTTCACC 13940 0.0 72.122986 6 CCTTCAC 15370 0.0 68.16122 5 GGCTGCG 40 3.9876395E-6 66.119 1 TTCACCG 15175 0.0 65.72752 7 GTGTGGC 5425 0.0 65.32271 7 CGGGGCA 1390 0.0 65.171776 94 ATGCAGC 80 0.0 64.542336 2 AGGCGCG 990 0.0 63.53113 5 AACGGGC 885 0.0 63.113274 5 CGTAGTG 2730 0.0 62.582905 4 CGGGCCC 915 0.0 62.06994 7 CAGCGTA 1390 0.0 61.647644 1 CGCTGCC 80 2.5465852E-11 59.507103 1 GCGCGCA 1050 0.0 59.453766 7 GCAGCCC 105 0.0 58.112705 4 GGCCCGA 990 0.0 57.841778 9 >>END_MODULE