FastQCFastQC Report
Tue 7 Mar 2017
1NF_32A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_32A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3477986
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC459.094947E-1294.035911
TCACCGG122400.079.225678
GGCGCGC8400.074.317216
CACCGGC129550.072.13589
CTTCACC139400.072.1229866
CCTTCAC153700.068.161225
GGCTGCG403.9876395E-666.1191
TTCACCG151750.065.727527
GTGTGGC54250.065.322717
CGGGGCA13900.065.17177694
ATGCAGC800.064.5423362
AGGCGCG9900.063.531135
AACGGGC8850.063.1132745
CGTAGTG27300.062.5829054
CGGGCCC9150.062.069947
CAGCGTA13900.061.6476441
CGCTGCC802.5465852E-1159.5071031
GCGCGCA10500.059.4537667
GCAGCCC1050.058.1127054
GGCCCGA9900.057.8417789
Produced by FastQC (version 0.11.5)