FastQCFastQC Report
Tue 7 Mar 2017
1NF_33A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_33A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2602951
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA150820.5794192821916355No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT59090.22701157263429086No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA58300.22397655583989096No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG57580.22121046458423535No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA54850.2107223685732079No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG45320.1741100773698775No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG43490.16707959542841952No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG42070.16162424878532095No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT39890.1532491391501415No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT39880.15321072121603518No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA39360.15121298864250615No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC39220.15067513756501755No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA36900.14176217685234951No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG36630.14072489263147867No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA36610.140648056763266No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32020.12301422500846156No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC31450.12082440276440086No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30670.1178278039041073No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC28990.11137359097424425No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC27850.10699394648612287No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA27410.10530355738544443No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA27140.1042662731645736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGCG1050.088.999872
GCTGCTT256.8162626E-788.999863
GCTGCTC202.4113102E-588.999863
GCTGCCA450.088.999863
TGCTGCC202.4113102E-588.999862
CTGCAGC850.088.999861
AGCTGCA850.088.999862
GCAGCTA301.9388608E-888.999863
GCAGCAA750.088.999863
TGCAGCC301.9388608E-888.999862
AGCAGCC301.9388608E-888.999862
CAGCTGC4050.086.8023451
CAGCAGC2900.085.930911
AGCTGCC1450.085.930912
AGCTGCT1150.085.130312
AGCAGCA1100.084.9544142
GCTGCCC950.084.315673
CAGCCCT148250.083.626861
CAGCGTA52050.082.24581
AGCCCTT127150.081.0903242
Produced by FastQC (version 0.11.5)