FastQCFastQC Report
Tue 7 Mar 2017
1NF_33A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_33A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2602951
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG99650.080.546848
GGCTGCA404.2467946E-879.488231
CACCGGC97450.078.511899
GGCGCGC3450.074.8295756
GAGCTGC502.3464963E-974.189011
CTTCACC112200.074.047456
CCTTCAC124550.071.415945
AAGCTGC459.606265E-870.65621
AGCTGCC605.456968E-1270.410532
GTGTGGC46500.068.237557
CGGGGCA12150.066.8343194
TTCACCG125700.066.1321957
CGTAGTG19550.064.345434
AGGCGCG4150.063.3387685
ACCTTCA51100.061.3599854
TGTGGCA50600.060.7603728
AACGGGC7200.059.325115
GCCTTCA38600.058.490784
GGGCCCG7300.057.8694468
TGTGTGG51150.057.721116
Produced by FastQC (version 0.11.5)