##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_33A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2602951
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.82236238792048	12.0	12.0	12.0	2.0	32.0
2	14.65656556731187	12.0	12.0	12.0	12.0	22.0
3	13.735236276057444	12.0	12.0	12.0	12.0	22.0
4	13.724306373804193	12.0	12.0	12.0	12.0	22.0
5	22.436110783491507	22.0	22.0	22.0	22.0	27.0
6	26.814158622271414	27.0	22.0	32.0	22.0	32.0
7	34.962837563980266	37.0	37.0	37.0	27.0	41.0
8	37.59152169979381	41.0	37.0	41.0	27.0	41.0
9	38.54792310727324	41.0	41.0	41.0	32.0	41.0
10-11	38.90554739601322	41.0	41.0	41.0	37.0	41.0
12-13	39.081236258385196	41.0	41.0	41.0	37.0	41.0
14-15	39.034501225724185	41.0	41.0	41.0	37.0	41.0
16-17	38.934987251008565	41.0	41.0	41.0	37.0	41.0
18-19	38.87760699298604	41.0	41.0	41.0	37.0	41.0
20-21	38.8970017107506	41.0	41.0	41.0	37.0	41.0
22-23	38.88001137939208	41.0	41.0	41.0	37.0	41.0
24-25	38.89323579275983	41.0	41.0	41.0	37.0	41.0
26-27	38.86208902895214	41.0	41.0	41.0	37.0	41.0
28-29	38.81548576980512	41.0	41.0	41.0	37.0	41.0
30-31	38.686656414200655	41.0	41.0	41.0	32.0	41.0
32-33	38.73228712334577	41.0	41.0	41.0	32.0	41.0
34-35	38.70810764397793	41.0	41.0	41.0	32.0	41.0
36-37	38.63254686699827	41.0	41.0	41.0	32.0	41.0
38-39	38.54519293678598	41.0	41.0	41.0	32.0	41.0
40-41	38.58595436487279	41.0	41.0	41.0	32.0	41.0
42-43	38.325320568846664	41.0	41.0	41.0	32.0	41.0
44-45	38.42943893296493	41.0	41.0	41.0	32.0	41.0
46-47	38.44835438700152	41.0	41.0	41.0	32.0	41.0
48-49	38.40015428642337	41.0	41.0	41.0	32.0	41.0
50-51	38.292587336450055	41.0	41.0	41.0	32.0	41.0
52-53	38.263244486738316	41.0	41.0	41.0	32.0	41.0
54-55	38.123571477142676	41.0	39.0	41.0	32.0	41.0
56-57	37.91136022153317	41.0	37.0	41.0	29.5	41.0
58-59	37.90750056378318	41.0	37.0	41.0	29.5	41.0
60-61	37.90546172402016	41.0	37.0	41.0	29.5	41.0
62-63	37.90195186156021	41.0	37.0	41.0	29.5	41.0
64-65	37.89859048441557	41.0	37.0	41.0	29.5	41.0
66-67	37.7619428871308	41.0	37.0	41.0	27.0	41.0
68-69	37.787975647639925	41.0	37.0	41.0	27.0	41.0
70-71	37.76331940170983	41.0	37.0	41.0	27.0	41.0
72-73	37.616881954366406	41.0	37.0	41.0	27.0	41.0
74-75	37.53117576934794	41.0	37.0	41.0	27.0	41.0
76-77	36.48562304860906	39.0	34.5	41.0	24.5	41.0
78-79	37.00296183062993	41.0	37.0	41.0	27.0	41.0
80-81	37.657425168587494	41.0	37.0	41.0	27.0	41.0
82-83	37.831150490347305	41.0	37.0	41.0	29.5	41.0
84-85	37.74865700506848	41.0	37.0	41.0	27.0	41.0
86-87	37.61346871301073	41.0	37.0	41.0	27.0	41.0
88-89	37.52210567928478	41.0	37.0	41.0	27.0	41.0
90-91	37.3666828534229	41.0	37.0	41.0	27.0	41.0
92-93	37.26456049307113	41.0	37.0	41.0	27.0	41.0
94-95	37.103756659268655	41.0	37.0	41.0	27.0	41.0
96-97	37.05323842054653	41.0	37.0	41.0	27.0	41.0
98-99	36.938087386201275	41.0	37.0	41.0	27.0	41.0
100	36.79114205376897	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3305160319264289
1101	2	-0.4172688415143959
1101	3	-0.09629381711128637
1101	4	0.018557897944949886
1101	5	-1.0331056899696094
1101	6	-0.8134292912988599
1101	7	-0.11718804906625024
1101	8	-0.0917245032240146
1101	9	-0.008581839943389014
1101	10-11	-0.10784029102421755
1101	12-13	0.0010663858758519495
1101	14-15	-0.08392489737647679
1101	16-17	-0.038723276228225245
1101	18-19	0.08235550744252862
1101	20-21	0.17204133147588863
1101	22-23	0.01261881800389375
1101	24-25	0.01128346830073923
1101	26-27	0.15927235935745188
1101	28-29	0.05515769978310203
1101	30-31	0.05970961195192359
1101	32-33	-0.06167302164983823
1101	34-35	0.01983724005014409
1101	36-37	0.10512445805750303
1101	38-39	0.14641465473531667
1101	40-41	0.11252982906957953
1101	42-43	0.18894435198525628
1101	44-45	0.05500517768269475
1101	46-47	0.07234737583637951
1101	48-49	0.14798439791143636
1101	50-51	0.23670933392843807
1101	52-53	0.05856856397606691
1101	54-55	0.16379417383060257
1101	56-57	0.2732877606255357
1101	58-59	0.13226666626351857
1101	60-61	0.1348920512863998
1101	62-63	-0.23202595338393905
1101	64-65	-0.05656341069450832
1101	66-67	0.1962089421421993
1101	68-69	0.08363083700924534
1101	70-71	-0.02301463359563627
1101	72-73	0.03630926744516927
1101	74-75	0.06441327915774764
1101	76-77	-0.22171705840479206
1101	78-79	0.11351206445442585
1101	80-81	0.10334488037606349
1101	82-83	0.2136387217795388
1101	84-85	0.12408381954627856
1101	86-87	0.014190119880581165
1101	88-89	-0.004004754352578743
1101	90-91	0.03198721635980206
1101	92-93	0.3683566741636142
1101	94-95	0.3626443358455518
1101	96-97	0.21238884991427653
1101	98-99	-0.17796703416391324
1101	100	-0.12397861859174952
1102	1	1.223272295073441
1102	2	0.7143895346668767
1102	3	-0.06581467774967997
1102	4	-0.05072788998152866
1102	5	-0.8225512840508102
1102	6	-0.004793971837493416
1102	7	0.2417441159095688
1102	8	-0.27894422586441436
1102	9	-0.18236932441475062
1102	10-11	-0.05819534935950088
1102	12-13	-0.18353974059635192
1102	14-15	-0.1185279195446185
1102	16-17	-0.1753818603124273
1102	18-19	0.056282201438456525
1102	20-21	0.05373146269396045
1102	22-23	-0.07750710282097373
1102	24-25	0.10280119957281642
1102	26-27	-0.03188726623500315
1102	28-29	-0.16255255416035652
1102	30-31	-0.3333348673138943
1102	32-33	-0.2648027297256377
1102	34-35	-0.0219922601796938
1102	36-37	-0.07674617847292353
1102	38-39	-0.08244432271262525
1102	40-41	-0.0873563084755844
1102	42-43	-0.06682552396220132
1102	44-45	-0.11460203387192536
1102	46-47	-0.45726483678053853
1102	48-49	-0.2981419717559177
1102	50-51	-0.06252051386952218
1102	52-53	-0.16290530262543257
1102	54-55	-0.10193510693347463
1102	56-57	-0.12221282121787169
1102	58-59	-0.013227222814286677
1102	60-61	-0.033449800371158744
1102	62-63	-0.22431731140019195
1102	64-65	-0.26097756016931584
1102	66-67	-0.14743324381188216
1102	68-69	-0.34724229264757156
1102	70-71	-0.4461432366531142
1102	72-73	-0.11629262350319891
1102	74-75	-0.1351860662612978
1102	76-77	-0.20997396035491533
1102	78-79	-0.33436724020425146
1102	80-81	-0.3028196978629367
1102	82-83	-0.3946009555551129
1102	84-85	-0.40992254968819
1102	86-87	-0.32639503858417385
1102	88-89	-0.43700162669505005
1102	90-91	-0.41359433370749343
1102	92-93	-0.24288421014723127
1102	94-95	-0.2111093715193917
1102	96-97	-0.26432915453655426
1102	98-99	-0.20620025226774885
1102	100	-0.5227187837862957
1103	1	1.7995389227017071
1103	2	0.6218359933843498
1103	3	0.43165479735811196
1103	4	1.0656018962018976
1103	5	2.9382380119915545
1103	6	-0.37301613286857105
1103	7	-0.447281329924067
1103	8	-0.24708145513717739
1103	9	-0.33801112850007087
1103	10-11	-0.2319510874815478
1103	12-13	-0.49237618712593445
1103	14-15	-0.3561939344094185
1103	16-17	-0.46806529722532986
1103	18-19	-0.30424265591452837
1103	20-21	-0.3416369099275727
1103	22-23	-0.17050795910969896
1103	24-25	-0.2824264837653345
1103	26-27	-0.31990875129758933
1103	28-29	-0.2957202647556798
1103	30-31	-0.36288699461626095
1103	32-33	-0.3928056947851104
1103	34-35	-0.5118521518248897
1103	36-37	-0.29127032244561235
1103	38-39	-0.3502849298527053
1103	40-41	-0.44397993995308127
1103	42-43	-0.1419209995742392
1103	44-45	-0.4440663828659339
1103	46-47	-0.7027449219679056
1103	48-49	-0.40777917023946486
1103	50-51	-0.2846835781980701
1103	52-53	-0.16876745292096018
1103	54-55	-0.3214732454756515
1103	56-57	-0.3462644843583007
1103	58-59	-0.4623088328229912
1103	60-61	-0.3743015113801107
1103	62-63	-0.5166478342010592
1103	64-65	-0.46942606259353425
1103	66-67	-0.47441034709284935
1103	68-69	-0.4393438714952609
1103	70-71	-0.4943916788931304
1103	72-73	-0.501370427267851
1103	74-75	-0.46549828051705333
1103	76-77	-0.43598306234390094
1103	78-79	-0.5055544026121339
1103	80-81	-0.4425550965135585
1103	82-83	-0.5617352697282598
1103	84-85	-0.5148998295714478
1103	86-87	-0.3452174150235692
1103	88-89	-0.2071976087606231
1103	90-91	-0.6151138104999774
1103	92-93	-0.39307922787136107
1103	94-95	-0.45309923780126127
1103	96-97	-0.1733432490257485
1103	98-99	-0.2598551063326866
1103	100	-0.1441961781124803
1104	1	1.6524186778253167
1104	2	-0.5965386584171295
1104	3	0.15302963876265174
1104	4	0.21600904281429223
1104	5	-1.1091248709595156
1104	6	-0.4336823818700779
1104	7	-0.25993966097870214
1104	8	-0.11469429107206963
1104	9	0.025091231384998025
1104	10-11	0.007838903092142857
1104	12-13	0.05877904547624979
1104	14-15	-0.004252387533043134
1104	16-17	0.01724431149625616
1104	18-19	0.037477247676349634
1104	20-21	0.14795818946789296
1104	22-23	-0.0831847889619155
1104	24-25	-0.09906378695601603
1104	26-27	-0.053996804804882004
1104	28-29	0.05863275999001871
1104	30-31	-0.06640616971602498
1104	32-33	0.0681304982084967
1104	34-35	-0.05983460988819189
1104	36-37	0.02984380513609608
1104	38-39	-0.08411819222911276
1104	40-41	-0.04004956081234212
1104	42-43	-0.1437253053712837
1104	44-45	0.058019058503454346
1104	46-47	-0.10245394088167359
1104	48-49	-0.2063735116072749
1104	50-51	-0.023061833611471627
1104	52-53	0.03426454230704934
1104	54-55	-0.1146834175836915
1104	56-57	0.03449874680090659
1104	58-59	-0.06900382072570466
1104	60-61	0.019360016005229852
1104	62-63	-0.09332813844282128
1104	64-65	-0.17693132475148587
1104	66-67	-0.5705788076813718
1104	68-69	-0.3170047081562686
1104	70-71	-0.17264528471644525
1104	72-73	-0.07604249666025709
1104	74-75	-0.12315889796153101
1104	76-77	-0.16664826141365552
1104	78-79	-0.07925413136052839
1104	80-81	-0.28657446602377235
1104	82-83	-0.11847831219973415
1104	84-85	-0.19542271096877073
1104	86-87	-0.10086800927341244
1104	88-89	-0.006981680488266306
1104	90-91	7.761566521580221E-4
1104	92-93	-0.08611091158115158
1104	94-95	-0.1922171566749924
1104	96-97	-0.26098322550419795
1104	98-99	-0.24182906648681524
1104	100	-0.27815054800151273
1105	1	1.296220721244012
1105	2	0.4158073603827983
1105	3	-0.16588701942711914
1105	4	-0.19339623981412046
1105	5	-1.1318348954492237
1105	6	0.36906212421757445
1105	7	0.1845408193163678
1105	8	0.2097847610131467
1105	9	0.07878132211909161
1105	10-11	-0.039770974689915306
1105	12-13	0.013795920854342114
1105	14-15	-0.0581311395826134
1105	16-17	0.051410141166236656
1105	18-19	-0.05030782012920554
1105	20-21	-0.07807898750423448
1105	22-23	0.017967244535562088
1105	24-25	-0.09955306041189971
1105	26-27	-0.1281482333587718
1105	28-29	-0.05234835137668625
1105	30-31	0.03032129567080233
1105	32-33	-0.1329313237521248
1105	34-35	-0.021991604741245396
1105	36-37	-0.0700440394776436
1105	38-39	-0.016270227400262627
1105	40-41	0.0520852242615959
1105	42-43	0.21897007624806974
1105	44-45	0.020924096867069863
1105	46-47	-0.2481002904572378
1105	48-49	-0.12598937894443196
1105	50-51	0.03506574978863597
1105	52-53	-0.0029962999536792267
1105	54-55	0.08806947551759237
1105	56-57	0.04943302404210215
1105	58-59	-0.018061081341080865
1105	60-61	0.010237325525643826
1105	62-63	-0.1542000047727896
1105	64-65	-0.15108955961799353
1105	66-67	-0.1644664972004506
1105	68-69	-0.2567947836226452
1105	70-71	-0.29877202234852973
1105	72-73	-0.0223966655546306
1105	74-75	-0.06922770383117438
1105	76-77	-0.26336419698129987
1105	78-79	-0.20513781296568823
1105	80-81	-0.10948183638629416
1105	82-83	-0.053078574298552894
1105	84-85	-0.07763398523508869
1105	86-87	-0.25298621372350993
1105	88-89	-0.055789357711354626
1105	90-91	-0.16424326458439964
1105	92-93	-0.1362248057385287
1105	94-95	0.01933791652913186
1105	96-97	-0.017996098547314432
1105	98-99	-0.2067078425258444
1105	100	-0.05652882686357685
1106	1	1.4621514712318078
1106	2	-0.62970901531971
1106	3	-0.13013751981840826
1106	4	-0.15173302497206365
1106	5	-1.2005018574934532
1106	6	-0.5586818174603998
1106	7	-0.16920309129518074
1106	8	-0.19233881381477147
1106	9	-0.22430804857876296
1106	10-11	-0.27632171059426014
1106	12-13	-0.40590847474842917
1106	14-15	-0.25338507799121146
1106	16-17	-0.1431700442263235
1106	18-19	-0.07503659917446726
1106	20-21	-0.27038753328503873
1106	22-23	-0.12839748486850766
1106	24-25	-0.30751834294620295
1106	26-27	-0.18485453067034285
1106	28-29	-0.2616105645485547
1106	30-31	-0.1737624409096199
1106	32-33	-0.3144353194856677
1106	34-35	-0.13009637180965683
1106	36-37	-0.23449756263174493
1106	38-39	-0.20304062879322515
1106	40-41	-0.2934282112260149
1106	42-43	-0.21141044303456624
1106	44-45	-0.22460363287281382
1106	46-47	-0.46899447452726406
1106	48-49	-0.5763698316561587
1106	50-51	-0.3764735396187504
1106	52-53	-0.47868950646697783
1106	54-55	-0.3543394813755327
1106	56-57	-0.397106934772701
1106	58-59	-0.48761848469123237
1106	60-61	-0.38705643781626975
1106	62-63	-0.5317032556518981
1106	64-65	-0.8014742469788487
1106	66-67	-0.491846573416737
1106	68-69	-0.6072587828178868
1106	70-71	-0.8170239148113723
1106	72-73	-0.6952011386755217
1106	74-75	-0.6496793800125502
1106	76-77	-0.6390243584379363
1106	78-79	-0.3864961848485109
1106	80-81	-0.547086408180057
1106	82-83	-0.43570787718284976
1106	84-85	-0.6669397764598628
1106	86-87	-0.5479263812750759
1106	88-89	-0.5946611251392397
1106	90-91	-0.653362527836812
1106	92-93	-0.5759499381776436
1106	94-95	-0.5045359811676633
1106	96-97	-0.5191794888110977
1106	98-99	-0.9335307202824339
1106	100	-0.8689934148414693
1107	1	1.4291779493422325
1107	2	-0.4669965134391614
1107	3	-0.06736107931893365
1107	4	-0.18700833714296827
1107	5	-1.1153544225604008
1107	6	-0.2616756492642196
1107	7	-0.2711174887677217
1107	8	-0.33795563343264234
1107	9	-0.31907636511253656
1107	10-11	-0.1838660915179844
1107	12-13	-0.1712830798334224
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2227	100	0.2270236400978547
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2228	2	1.7905657310796048
2228	3	-0.1387843420465451
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2228	14-15	0.20918331286437564
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2228	66-67	0.3314325647845422
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2228	82-83	0.2217970025971212
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2228	90-91	0.27028259386194264
2228	92-93	0.4087996403868104
2228	94-95	0.575046945517343
2228	96-97	0.12642833953894694
2228	98-99	0.43186313710969415
2228	100	0.571777886137447
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	24.0
13	34.0
14	137.0
15	464.0
16	1676.0
17	4273.0
18	6868.0
19	8942.0
20	10690.0
21	12039.0
22	14063.0
23	16275.0
24	17939.0
25	20625.0
26	22989.0
27	26043.0
28	29577.0
29	33742.0
30	38570.0
31	44600.0
32	51718.0
33	62058.0
34	76504.0
35	98531.0
36	138553.0
37	228507.0
38	562999.0
39	1073147.0
40	1364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.655643055541105	18.215702490802098	21.211695638755675	34.91695881490112
2	18.701369454600222	53.4050794763154	11.252741291261524	16.640809777822856
3	41.499705526535074	14.36861469923944	23.73248670451345	20.39919306971203
4	17.15272396599091	16.985221773287318	21.88320102837126	43.97885323235051
5	17.04138879295077	35.871286090287526	26.22769310678534	20.85963200997637
6	17.662952548857046	27.89603031328673	30.983333916005336	23.457683221850893
7	20.212904507230448	26.63503846211473	36.74832910800088	16.40372792265394
8	20.32931084757262	27.605475477640567	34.81529233550689	17.249921339279915
9	20.84822188354679	28.201299217695606	27.800907508439458	23.149571390318144
10-11	19.41200583491583	30.310021202857833	28.81579407372632	21.462178888500013
12-13	23.831969944881788	26.810954182387604	27.468419497716244	21.88865637501436
14-15	21.86243613498679	27.460947209532566	28.518861861018515	22.157754794462132
16-17	20.95360227680045	29.762181462501598	28.233224520937966	21.05099173975999
18-19	21.288798751878158	29.638494923646274	29.911108584064777	19.161597740410787
20-21	22.755557058123646	29.06220670308431	29.818924751176645	18.3633114876154
22-23	21.97350238248818	30.33312959022279	28.720248671603883	18.973119355685146
24-25	22.09526802463819	28.423739056171243	26.561237610696477	22.91975530849409
26-27	20.05056952666416	26.43977931201932	30.06138033332168	23.44827082799484
28-29	19.496450264614193	28.195189248467262	30.73698764916063	21.571372837757913
30-31	20.104734028819436	30.03579376398554	28.40522951656134	21.45424269063368
32-33	20.523724803117695	30.166395756201325	28.862606326434882	20.4472731142461
34-35	21.310063078406007	29.261384482458563	30.062148692003802	19.36640374713162
36-37	21.816872465136687	28.198456290571738	28.86567976116339	21.118991483128188
38-39	21.517022660062565	29.29847258519494	28.924547456661777	20.259957298080717
40-41	20.843735947226786	29.89158486563988	29.80831272980276	19.45636645733057
42-43	20.393984278970006	30.037882069416334	29.38685063254277	20.181283019070886
44-45	20.57501274514964	30.050143087595576	28.81744604489289	20.55739812236189
46-47	19.859767843380723	28.21558893202884	28.9490982743081	22.97554495028234
48-49	20.86266702677077	28.099088304005722	30.395385852442093	20.642858816781416
50-51	21.85586666825461	30.631291176822	28.32558123452958	19.187260920393815
52-53	20.458508665470315	30.46211231476071	28.20879421049538	20.870584809273595
54-55	22.328133654750076	28.39610111524335	28.184933618937485	21.09083161106909
56-57	21.50536994032204	28.812807476727443	29.413486941700643	20.26833564124987
58-59	21.22396143522087	28.097404807994526	29.07510695148126	21.60352680530335
60-61	19.583302278729224	30.438903363962776	29.663261801453896	20.314532555854107
62-63	20.46621453653075	31.280625118389267	27.25510183302128	20.998058512058705
64-65	19.86688943963174	30.24112266510024	28.92465430555881	20.96733358970921
66-67	20.08723944476865	30.298322941922457	29.58688042917443	20.02755718413447
68-69	20.975327618537577	29.981010015171243	29.248841026972848	19.794821339318336
70-71	19.585174672900106	30.84604742847637	29.524643375922178	20.04413452270135
72-73	20.13570366864378	30.80499786588376	28.560237976051027	20.49906048942143
74-75	19.977825168433828	29.241483992591487	30.594141034541178	20.186549804433508
76-77	19.557052745134275	30.36543907280621	28.754671140563154	21.322837041496363
78-79	19.509049536468414	29.57470194406272	28.364287303141705	22.55196121632716
80-81	20.643163430366318	29.678654555520062	28.440250308640614	21.23793170547301
82-83	20.154547665322937	28.45199544670645	30.12500043220176	21.268456455768856
84-85	20.420899202482108	29.111477703575673	28.44957511685775	22.01804797708447
86-87	21.547831672590075	28.938174402822025	29.056847401276475	20.457146523311426
88-89	21.32227998145182	28.48453543689451	29.49704009026678	20.696144491386892
90-91	21.19156296065504	29.126729623415887	29.47143453718491	20.210272878744163
92-93	21.237357138109783	29.25153028236029	30.215282577351626	19.295830002178295
94-95	20.905406978464057	29.96310725787769	29.451649301120153	19.6798364625381
96-97	20.71070488841319	29.549307689618438	29.647427093325994	20.092560328642374
98-99	21.798297393996275	29.66582928376293	29.226769923828762	19.309103398412034
100	21.854118652252772	31.257138532381134	28.014280714466004	18.874462100900093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	8.0
2	18.0
3	26.0
4	26.0
5	23.0
6	37.5
7	51.0
8	51.0
9	60.0
10	76.5
11	93.0
12	113.0
13	126.5
14	133.0
15	169.5
16	232.5
17	309.5
18	467.5
19	744.0
20	1191.0
21	1867.5
22	2887.5
23	4407.5
24	6773.5
25	10088.0
26	14374.0
27	19510.5
28	25406.5
29	32033.5
30	39576.5
31	48503.0
32	58410.5
33	68860.5
34	80375.0
35	93053.0
36	105749.5
37	115375.5
38	121077.5
39	124830.0
40	126578.0
41	126340.5
42	124423.0
43	122230.5
44	120625.5
45	117094.0
46	108806.0
47	98221.5
48	90733.5
49	87000.0
50	84666.5
51	79846.5
52	70420.0
53	58514.0
54	47076.0
55	37659.0
56	29215.5
57	21164.5
58	14723.0
59	10667.5
60	8457.5
61	7017.0
62	5732.0
63	4473.0
64	3652.5
65	3461.5
66	3105.0
67	2235.0
68	1747.0
69	1970.0
70	2451.0
71	2301.0
72	1380.5
73	601.0
74	259.0
75	156.0
76	98.0
77	70.0
78	59.0
79	42.5
80	40.5
81	34.5
82	24.0
83	22.0
84	18.0
85	14.0
86	15.0
87	15.0
88	11.5
89	8.5
90	9.0
91	9.5
92	12.0
93	15.5
94	13.0
95	15.0
96	17.5
97	17.0
98	39.0
99	87.0
100	90.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.367981955864709
2	0.0062621232593314275
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	4.033883081164417E-4
30-31	0.010103916669964207
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.02821451498703E-4
40-41	0.0015175083971999473
42-43	1.7288070347847503E-4
44-45	0.0
46-47	0.0020361505076353726
48-49	0.0
50-51	0.0
52-53	0.0013062097596151445
54-55	0.01976602709770564
56-57	0.01210164924349325
58-59	0.01417621768523495
60-61	3.4576140695695006E-4
62-63	0.011832723704748955
64-65	7.683586821265556E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.7288070347847503E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2602951.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.95128058112424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.7801983937014	63.43555519717115
2	8.871341842574262	13.12094179462554
3	2.4455621675393067	5.425573620908448
4	1.0089103845485277	2.9844085971583243
5	0.5424116053116613	2.005601640743034
6	0.34671735902143186	1.5384115619604177
7	0.22312481634398468	1.1550256128646077
8	0.15944901890921917	0.9433167308592518
9	0.1080508441918183	0.7191448466270844
>10	0.48130527984004223	6.08823707924763
>50	0.02294163594277445	1.1486390575062713
>100	0.009510558689280437	1.2068417845507786
>500	4.2319412117913503E-4	0.18628793517619938
>1k	5.289926513617586E-5	0.04201454060118551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.683586821265556E-5	0.0
3	0.0	0.0	0.0	7.683586821265556E-5	0.0
4	0.0	0.0	0.0	7.683586821265556E-5	0.0
5	0.0	0.0	0.0	7.683586821265556E-5	0.0
6	0.0	0.0	0.0	7.683586821265556E-5	0.0
7	0.0	0.0	0.0	7.683586821265556E-5	0.0
8	0.0	0.0	0.0	7.683586821265556E-5	0.0
9	0.0	0.0	0.0	7.683586821265556E-5	0.0
10-11	0.0	0.0	0.0	7.683586821265556E-5	0.0
12-13	0.0	0.0	0.0	7.683586821265556E-5	0.0
14-15	0.0	0.0	0.0	7.683586821265556E-5	0.0
16-17	0.0	0.0	0.0	7.683586821265556E-5	0.0
18-19	0.0	0.0	0.0	7.683586821265556E-5	0.0
20-21	0.0	0.0	0.0	1.1525380231898333E-4	0.0
22-23	0.0	0.0	0.0	1.1525380231898333E-4	0.0
24-25	0.0	0.0	0.0	1.1525380231898333E-4	0.0
26-27	0.0	0.0	0.0	1.1525380231898333E-4	0.0
28-29	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
30-31	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
32-33	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
34-35	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
36-37	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
38-39	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
40-41	0.0	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
42-43	3.841793410632778E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
44-45	3.841793410632778E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
46-47	3.841793410632778E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
48-49	3.841793410632778E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
50-51	3.841793410632778E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
52-53	5.762690115949167E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
54-55	7.683586821265556E-5	0.0	0.0	1.1525380231898333E-4	3.841793410632778E-5
56-57	7.683586821265556E-5	0.0	1.536717364253111E-4	1.1525380231898333E-4	3.841793410632778E-5
58-59	1.1525380231898333E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
60-61	1.3446276937214722E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
62-63	1.536717364253111E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
64-65	1.72880703478475E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
66-67	2.3050760463796667E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
68-69	3.073434728506222E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
70-71	4.4180624222276945E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
72-73	5.186421104354251E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
74-75	6.146869457012444E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
76-77	7.299407480202278E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
78-79	8.259855832860473E-4	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
80-81	0.0010372842208708501	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
82-83	0.001210164924349325	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
84-85	0.0013830456278278	0.0	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
86-87	0.0015367173642531111	3.841793410632778E-5	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
88	0.0015367173642531111	3.841793410632778E-5	3.073434728506222E-4	1.1525380231898333E-4	3.841793410632778E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9965	0.0	80.54684	8
GGCTGCA	40	4.2467946E-8	79.48823	1
CACCGGC	9745	0.0	78.51189	9
GGCGCGC	345	0.0	74.829575	6
GAGCTGC	50	2.3464963E-9	74.18901	1
CTTCACC	11220	0.0	74.04745	6
CCTTCAC	12455	0.0	71.41594	5
AAGCTGC	45	9.606265E-8	70.6562	1
AGCTGCC	60	5.456968E-12	70.41053	2
GTGTGGC	4650	0.0	68.23755	7
CGGGGCA	1215	0.0	66.83431	94
TTCACCG	12570	0.0	66.132195	7
CGTAGTG	1955	0.0	64.34543	4
AGGCGCG	415	0.0	63.338768	5
ACCTTCA	5110	0.0	61.359985	4
TGTGGCA	5060	0.0	60.760372	8
AACGGGC	720	0.0	59.32511	5
GCCTTCA	3860	0.0	58.49078	4
GGGCCCG	730	0.0	57.869446	8
TGTGTGG	5115	0.0	57.72111	6
>>END_MODULE