FastQCFastQC Report
Tue 7 Mar 2017
1NF_4A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_4A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3582899
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA194790.5436658973641176No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA73540.20525278552367793No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT71150.19858220954595707No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG70680.19727042263820443No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA70570.19696340868107085No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG66420.18538060938921247No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG65640.1832036013295379No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG62210.17363034793891763No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG58710.16386172203012142No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA53810.15018564575780674No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC52260.14585953999819698No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT52230.14577580891897873No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT49580.13837956358803302No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA49560.1383237428685542No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA44050.12294513465213504No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA43990.12277767249369853No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC43960.12269394141448028No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA43250.12071230587298162No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41570.11602336543675945No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA41500.11582799291858352No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA41440.11566053076014701No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG39620.110580845287573No Hit
CAGCTCATCAACTTCAGATTCAGCCGCACCACATTGACGGGTACGAGGTA37300.10410564182802809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC202.281365E-589.999813
AGCTGCA1400.089.999812
GCAGCTT401.4551915E-1189.999813
GCAGCTC202.281365E-589.999813
GCAGCAA1000.089.999813
AGCAGCG1000.089.999812
AGCAGCC850.089.999812
GCTGCAA650.089.99983
AGCTGCT1200.089.99982
GCAGCCG158.2057243E-489.99983
CAGCTGC5100.089.1174551
CAGCAGC4450.087.977341
AGCTGCC1550.087.096582
GCTGCCC1450.086.8963553
TGCTGCA850.084.70572
GCTGCAG750.083.999823
CAGCCCT197350.083.706431
AGCCCTT169950.081.897442
CAGCGTA69900.081.308841
GCCCTTC154950.081.1711653
Produced by FastQC (version 0.11.5)