##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_4A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3582899
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.788840265941072	12.0	12.0	12.0	2.0	27.0
2	14.62298630243275	12.0	12.0	12.0	12.0	22.0
3	13.716148013103355	12.0	12.0	12.0	12.0	22.0
4	13.786321635078187	12.0	12.0	12.0	12.0	22.0
5	22.425990238630785	22.0	22.0	22.0	22.0	27.0
6	26.861332122395858	27.0	22.0	32.0	22.0	32.0
7	34.95820172435784	37.0	37.0	37.0	27.0	41.0
8	37.55120336911534	41.0	37.0	41.0	27.0	41.0
9	38.51134402616429	41.0	37.0	41.0	32.0	41.0
10-11	38.862385319820625	41.0	41.0	41.0	37.0	41.0
12-13	39.043266500116246	41.0	41.0	41.0	37.0	41.0
14-15	38.99750802353066	41.0	41.0	41.0	37.0	41.0
16-17	38.90278123943767	41.0	41.0	41.0	37.0	41.0
18-19	38.841369098040445	41.0	41.0	41.0	37.0	41.0
20-21	38.85472881038511	41.0	41.0	41.0	37.0	41.0
22-23	38.83586043033867	41.0	41.0	41.0	34.5	41.0
24-25	38.86039768355178	41.0	41.0	41.0	37.0	41.0
26-27	38.82882367044117	41.0	41.0	41.0	37.0	41.0
28-29	38.77670093407601	41.0	41.0	41.0	34.5	41.0
30-31	38.65613808817943	41.0	41.0	41.0	32.0	41.0
32-33	38.69748993761756	41.0	41.0	41.0	32.0	41.0
34-35	38.67149227483108	41.0	41.0	41.0	32.0	41.0
36-37	38.59315487263247	41.0	41.0	41.0	32.0	41.0
38-39	38.50556686080183	41.0	41.0	41.0	32.0	41.0
40-41	38.54766503325938	41.0	41.0	41.0	32.0	41.0
42-43	38.29228203195234	41.0	41.0	41.0	32.0	41.0
44-45	38.397515670969234	41.0	41.0	41.0	32.0	41.0
46-47	38.40704608195766	41.0	41.0	41.0	32.0	41.0
48-49	38.355939282687004	41.0	41.0	41.0	32.0	41.0
50-51	38.24621444813265	41.0	41.0	41.0	32.0	41.0
52-53	38.207420862268236	41.0	41.0	41.0	32.0	41.0
54-55	38.074743664278564	41.0	37.0	41.0	32.0	41.0
56-57	37.877636098589434	41.0	37.0	41.0	27.0	41.0
58-59	37.86162950727888	41.0	37.0	41.0	29.5	41.0
60-61	37.85580601071925	41.0	37.0	41.0	27.0	41.0
62-63	37.841052873664594	41.0	37.0	41.0	27.0	41.0
64-65	37.84191530378054	41.0	37.0	41.0	27.0	41.0
66-67	37.70891909037905	41.0	37.0	41.0	27.0	41.0
68-69	37.72089389067345	41.0	37.0	41.0	27.0	41.0
70-71	37.70997689301317	41.0	37.0	41.0	27.0	41.0
72-73	37.54948046260863	41.0	37.0	41.0	27.0	41.0
74-75	37.48825573927705	41.0	37.0	41.0	27.0	41.0
76-77	36.401253705449136	39.0	34.5	41.0	24.5	41.0
78-79	36.94008706357617	41.0	37.0	41.0	27.0	41.0
80-81	37.592325655844604	41.0	37.0	41.0	27.0	41.0
82-83	37.76540854207724	41.0	37.0	41.0	27.0	41.0
84-85	37.68803209356446	41.0	37.0	41.0	27.0	41.0
86-87	37.548574213227894	41.0	37.0	41.0	27.0	41.0
88-89	37.44740306662287	41.0	37.0	41.0	27.0	41.0
90-91	37.296485890336285	41.0	37.0	41.0	27.0	41.0
92-93	37.19107920708901	41.0	37.0	41.0	27.0	41.0
94-95	37.032066491408216	41.0	37.0	41.0	27.0	41.0
96-97	36.969889327050524	41.0	37.0	41.0	27.0	41.0
98-99	36.84632639658555	41.0	37.0	41.0	27.0	41.0
100	36.71849080869988	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.601529258891608
1101	2	-0.6315988980561063
1101	3	-0.0750105341030256
1101	4	0.19267316366364895
1101	5	-1.0961436592328013
1101	6	-0.8084623375539088
1101	7	-0.33343473552766767
1101	8	-0.3455951589142998
1101	9	-0.2960408813520061
1101	10-11	-0.16354438342660416
1101	12-13	-0.1693445108239473
1101	14-15	-0.12437019701689422
1101	16-17	-0.2348095349128272
1101	18-19	-0.24747779086412436
1101	20-21	-0.14114354029901932
1101	22-23	-0.006919096742066699
1101	24-25	-0.24420895813786814
1101	26-27	-0.06169981115770895
1101	28-29	-0.2442023053150848
1101	30-31	-0.15552969505075964
1101	32-33	-0.215933371633227
1101	34-35	-0.2561527694350261
1101	36-37	-0.1027938311439911
1101	38-39	-0.1228585678235774
1101	40-41	-0.11348603663166301
1101	42-43	0.053936687928462845
1101	44-45	-0.13052570867634472
1101	46-47	-0.058358874871117905
1101	48-49	-0.08348792832771323
1101	50-51	-0.0868196086005284
1101	52-53	-0.07061997995742786
1101	54-55	-0.0714271726586162
1101	56-57	0.028563675614634576
1101	58-59	-0.049907337612673075
1101	60-61	-0.011201014224027972
1101	62-63	-0.12329733041946866
1101	64-65	-0.1353046895519796
1101	66-67	-0.0843125094299566
1101	68-69	-0.02138241459914525
1101	70-71	-0.17034315261064137
1101	72-73	0.006589734004393222
1101	74-75	0.03454496233687365
1101	76-77	-0.37079386239281575
1101	78-79	-0.34988341709353676
1101	80-81	-0.28097687937741966
1101	82-83	-0.171395715784044
1101	84-85	-0.06225349614081921
1101	86-87	-0.19297825564549953
1101	88-89	-0.3329211652411104
1101	90-91	-0.19519372663857837
1101	92-93	-0.1059979919706322
1101	94-95	-0.12098248673122924
1101	96-97	-0.23858953294745078
1101	98-99	-0.3877454443388757
1101	100	-0.6147945941943149
1102	1	1.716921327520069
1102	2	0.9278401027358179
1102	3	-0.2250583147061782
1102	4	-0.0903314332266838
1102	5	-0.8034503419716827
1102	6	-0.20707414242708566
1102	7	-0.09809671594402403
1102	8	-0.2062487236671231
1102	9	-0.18570945450827026
1102	10-11	-0.2516681348411183
1102	12-13	-0.2991466948559065
1102	14-15	-0.24723408720722517
1102	16-17	-0.26577847747112315
1102	18-19	-0.07584629855485758
1102	20-21	-0.09171790575959449
1102	22-23	-0.1264467377291183
1102	24-25	-0.17397628879979976
1102	26-27	-0.20294500516205716
1102	28-29	-0.15028682568097196
1102	30-31	-0.1166726074754294
1102	32-33	-0.012011458676852271
1102	34-35	0.0799601093967226
1102	36-37	-0.12395963018456513
1102	38-39	-0.11566302055265965
1102	40-41	-0.0180741167791183
1102	42-43	-0.17901950260714017
1102	44-45	-0.07661030651622269
1102	46-47	-0.2546754229313706
1102	48-49	-0.3315731036469174
1102	50-51	-0.24332251342877953
1102	52-53	-0.13490363891169466
1102	54-55	-0.049628073283997765
1102	56-57	-0.06348175037469161
1102	58-59	-0.08643518907076952
1102	60-61	-0.05631085001588332
1102	62-63	-0.25838830725046336
1102	64-65	-0.207163419079599
1102	66-67	-0.07736631919929238
1102	68-69	-0.21070067612615873
1102	70-71	-0.2769668523261615
1102	72-73	-0.03203075987649129
1102	74-75	-0.3404578311969857
1102	76-77	-0.13497992396497693
1102	78-79	-0.21724126446039804
1102	80-81	-0.33790199767177853
1102	82-83	-0.31011982618132805
1102	84-85	-0.36333764552540515
1102	86-87	-0.2329954667938523
1102	88-89	-0.2821229992296139
1102	90-91	-0.4234746589051426
1102	92-93	-0.3723400657292544
1102	94-95	-0.15545543951290597
1102	96-97	-0.21924293281969653
1102	98-99	-0.1177494779534527
1102	100	-0.5654650075568739
1103	1	1.5545970754995864
1103	2	0.8817008613887136
1103	3	0.4854555504669307
1103	4	1.1735651622262218
1103	5	2.8020337000760094
1103	6	0.029538763930244016
1103	7	-0.4738047405464414
1103	8	-0.11804380297916595
1103	9	-0.089262376377512
1103	10-11	-0.21309467859789066
1103	12-13	-0.3682909239253007
1103	14-15	-0.30444799315274196
1103	16-17	-0.3057642020192759
1103	18-19	-0.28442311673342147
1103	20-21	-0.09380649531021135
1103	22-23	-0.07752102423753371
1103	24-25	-0.25065472734944905
1103	26-27	-0.07624388121291759
1103	28-29	-0.23232801092156308
1103	30-31	-0.08839443951559645
1103	32-33	-0.29257732655177904
1103	34-35	-0.3327362547059849
1103	36-37	-0.2366896136405714
1103	38-39	-0.12172160610778349
1103	40-41	-0.18149871286149022
1103	42-43	-0.09416824014422076
1103	44-45	-0.3541164044946754
1103	46-47	-0.39181009347703366
1103	48-49	-0.3260184507386441
1103	50-51	-0.1902952899275192
1103	52-53	-0.1804955183561674
1103	54-55	-0.2221456314766428
1103	56-57	-0.28858033478532263
1103	58-59	-0.34301277977915845
1103	60-61	-0.1493292139489597
1103	62-63	-0.12482505432767965
1103	64-65	-0.4535980304592826
1103	66-67	-0.43459907855114466
1103	68-69	-0.33254242400553125
1103	70-71	-0.4022545839190599
1103	72-73	-0.432533466285534
1103	74-75	-0.2793011108517547
1103	76-77	-0.5099868470992845
1103	78-79	-0.5508565813968929
1103	80-81	-0.11056384318013102
1103	82-83	-0.34886858333821635
1103	84-85	-0.5550118995385205
1103	86-87	-0.41202817975303674
1103	88-89	-0.28721934575624886
1103	90-91	-0.5970801455018488
1103	92-93	-0.26914459218176034
1103	94-95	-0.23254262399936465
1103	96-97	-0.18824997653405262
1103	98-99	-0.29927799360280005
1103	100	-0.2807820462334334
1104	1	1.6772378440391567
1104	2	-0.5427644644746348
1104	3	0.051021403941382104
1104	4	-0.005290208279573605
1104	5	-1.1021380280058928
1104	6	-0.5622290545462434
1104	7	-0.3177846397159101
1104	8	-0.17851193161713752
1104	9	-0.09175162373868773
1104	10-11	-0.11667831357626568
1104	12-13	-0.1213746899650161
1104	14-15	-0.08617447376331455
1104	16-17	-0.06384808535123199
1104	18-19	-0.06294313341952318
1104	20-21	-0.05108192911302467
1104	22-23	-0.06286678646882393
1104	24-25	-0.17433394020631
1104	26-27	-0.21436661377538258
1104	28-29	-0.15793846604606188
1104	30-31	-0.10856146366861452
1104	32-33	-0.2745812288136804
1104	34-35	-0.30280113261495956
1104	36-37	-0.35350799527281396
1104	38-39	-0.21649419684863602
1104	40-41	-0.2106859184197134
1104	42-43	-0.24653167882638627
1104	44-45	-0.1100009562904134
1104	46-47	-0.3594516116670192
1104	48-49	-0.4335802306025158
1104	50-51	-0.20156034285692215
1104	52-53	-0.1315380934002519
1104	54-55	-0.12578617869085207
1104	56-57	-0.07906441516824714
1104	58-59	-0.0589183217866065
1104	60-61	-0.137085484749214
1104	62-63	-0.2547711916901534
1104	64-65	-0.2920296495919956
1104	66-67	-0.6223682005014197
1104	68-69	-0.5425880455881256
1104	70-71	-0.40303733114815543
1104	72-73	-0.2945938626109168
1104	74-75	-0.4129233400775192
1104	76-77	-0.4444902470444134
1104	78-79	-0.294245582648351
1104	80-81	-0.46869857014928584
1104	82-83	-0.3677145398666539
1104	84-85	-0.17579486095345231
1104	86-87	-0.2695200942378051
1104	88-89	-0.410233205619555
1104	90-91	-0.40681769646023014
1104	92-93	-0.41989735339413414
1104	94-95	-0.3430398207147789
1104	96-97	-0.38312614142451196
1104	98-99	-0.40546097766023337
1104	100	-0.09017644088817178
1105	1	1.4910227312866127
1105	2	0.3671784109488119
1105	3	-0.1749632506584362
1105	4	-0.14177570066428302
1105	5	-1.1893125945036829
1105	6	0.4207309709198839
1105	7	0.02804512366132883
1105	8	-0.016437673626903404
1105	9	0.11884348464280237
1105	10-11	0.020656297154665992
1105	12-13	-0.013672463500199683
1105	14-15	0.012618122213730487
1105	16-17	-0.07064513988009935
1105	18-19	-0.05022288824470422
1105	20-21	0.00551017298271006
1105	22-23	-0.04276969884032411
1105	24-25	-0.042919550656925765
1105	26-27	-0.04735418597296359
1105	28-29	0.0021295723019960633
1105	30-31	-0.08879764892834885
1105	32-33	-0.06688352583297075
1105	34-35	0.021620277144208444
1105	36-37	-0.17392656701586162
1105	38-39	-0.13683316714924132
1105	40-41	0.01225077893405313
1105	42-43	0.07725278081819908
1105	44-45	-0.05227639945328377
1105	46-47	-0.1276984755166808
1105	48-49	-0.08030983077799192
1105	50-51	-0.13338777500663923
1105	52-53	-0.1645549742114909
1105	54-55	-0.1229396501135156
1105	56-57	0.025240793509873072
1105	58-59	0.0102088298872971
1105	60-61	-0.1274773372515554
1105	62-63	-0.1927418023070615
1105	64-65	-0.23031403361422065
1105	66-67	-0.11010535182690973
1105	68-69	-0.08215883128465151
1105	70-71	-0.2712019705284803
1105	72-73	-0.2681549393466298
1105	74-75	-0.35400797852344823
1105	76-77	-0.32262629840110435
1105	78-79	-0.29616138209943443
1105	80-81	-0.3574684714997147
1105	82-83	-0.25618957340179804
1105	84-85	-0.12682982817848654
1105	86-87	-0.1530213738789996
1105	88-89	-0.05530624058638267
1105	90-91	-0.14162304608893095
1105	92-93	-0.003898255319462862
1105	94-95	-0.08659494466417783
1105	96-97	-0.11159658416768536
1105	98-99	-0.3013207722208051
1105	100	-0.32224156876518606
1106	1	1.1632446398305802
1106	2	-0.6595218820266773
1106	3	-0.06759706580156788
1106	4	-0.20352718593081498
1106	5	-1.1252477321143353
1106	6	-0.5922458984456718
1106	7	-0.276393737310201
1106	8	-0.1539012869494485
1106	9	-0.055104830591297116
1106	10-11	-0.17806534633117366
1106	12-13	0.012084638175466011
1106	14-15	-0.11396007150140264
1106	16-17	-0.061277334875050826
1106	18-19	0.054737580453348755
1106	20-21	-0.013263666564590437
1106	22-23	-0.026501364346380285
1106	24-25	-0.08767732046727161
1106	26-27	-0.16036324656933232
1106	28-29	-0.10809010442119416
1106	30-31	0.004075228254066587
1106	32-33	-0.07380679699527093
1106	34-35	-0.05214312475919769
1106	36-37	-0.22184288079449743
1106	38-39	-0.04622798769391068
1106	40-41	-0.08610460598053038
1106	42-43	0.028715776194580656
1106	44-45	-0.044155857605467475
1106	46-47	-0.24543329505758038
1106	48-49	-0.20268414774359655
1106	50-51	-0.07991112207908202
1106	52-53	-0.07185128723729406
1106	54-55	-0.16227568735340014
1106	56-57	-0.06004440275860645
1106	58-59	-0.13587819073391216
1106	60-61	-0.13717450442324974
1106	62-63	-0.22443840172110896
1106	64-65	-0.40982053145925335
1106	66-67	-0.321241988505939
1106	68-69	-0.4255835861450876
1106	70-71	-0.3482995762223595
1106	72-73	-0.27850240074852195
1106	74-75	-0.46709761522129867
1106	76-77	-0.4395118340694566
1106	78-79	-0.28113972261600395
1106	80-81	-0.0909787977878409
1106	82-83	-0.16341138784229514
1106	84-85	-0.3701671060158631
1106	86-87	-0.30280548482072334
1106	88-89	-0.29995312513396044
1106	90-91	-0.36190869719087715
1106	92-93	-0.23539006204346435
1106	94-95	-0.398463863227029
1106	96-97	-0.1798417974375326
1106	98-99	-0.5482071234526273
1106	100	-0.7413725702143807
1107	1	1.5485080250540282
1107	2	-0.5786129471052828
1107	3	-0.051914654597563725
1107	4	-0.18562746342754544
1107	5	-1.2065323822258662
1107	6	-0.34599920444350474
1107	7	-0.38697825547765774
1107	8	-0.30145765901990274
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2221	14-15	0.12737458938962476
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2221	82-83	0.28359989116239603
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2221	94-95	0.5200300859720741
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2222	4	0.6993572898697824
2222	5	3.197134174223674
2222	6	0.28943339778287935
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2222	9	0.23627687251238427
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2222	28-29	0.16443348527772628
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2222	64-65	0.23111710463516744
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2222	72-73	0.35367786810147095
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2222	92-93	0.3170057803628268
2222	94-95	0.5706477124196923
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2223	1	1.3536662069676009
2223	2	-0.857440996049279
2223	3	-0.018258370167419713
2223	4	-0.002383320559577129
2223	5	-1.1118534970483864
2223	6	0.0019225994190819051
2223	7	0.11075263415351344
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2223	9	0.0819666805732524
2223	10-11	0.05103941662655842
2223	12-13	0.0528673774635422
2223	14-15	0.06744831561616849
2223	16-17	0.08478201897691662
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2223	24-25	0.17885989546667957
2223	26-27	0.12439381448443498
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2223	30-31	0.1512453937759446
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2223	40-41	0.16851024353874777
2223	42-43	0.27604038427212174
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2223	46-47	0.18027937499046232
2223	48-49	0.23411302921693533
2223	50-51	0.25995578782597306
2223	52-53	0.26468146011265503
2223	54-55	0.2303164829503217
2223	56-57	0.32857326150791977
2223	58-59	0.22447280041645
2223	60-61	0.32807707224981897
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2223	64-65	0.13952852944708383
2223	66-67	0.24560250016366325
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2223	76-77	0.4254966193858678
2223	78-79	0.5898287623497538
2223	80-81	0.3323014883858093
2223	82-83	0.18897907426457294
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2223	86-87	0.37883957601189877
2223	88-89	0.44002423250478273
2223	90-91	0.36098957111764207
2223	92-93	0.30850053135684874
2223	94-95	0.41873246884864557
2223	96-97	0.5053049978506436
2223	98-99	0.5174119597045035
2223	100	0.7512991823458393
2224	1	1.3971215483533133
2224	2	-0.9300843001220702
2224	3	-0.057198573234046535
2224	4	-0.2874692304248878
2224	5	-1.0736371676801255
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2224	7	0.3547656829796537
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2224	9	0.24859667606575897
2224	10-11	0.2429024268715736
2224	12-13	0.23637298891716796
2224	14-15	0.2102573275572226
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2224	18-19	0.19146283072281278
2224	20-21	0.2314814501761333
2224	22-23	0.23391438149926813
2224	24-25	0.25055032199828275
2224	26-27	0.2062030021415211
2224	28-29	0.10378829292373837
2224	30-31	0.0777913129618426
2224	32-33	0.07105812340701334
2224	34-35	0.13577919284070106
2224	36-37	0.16665015198285715
2224	38-39	0.07671906606653067
2224	40-41	0.12792308456157286
2224	42-43	0.39199731092212886
2224	44-45	0.2503424791009792
2224	46-47	0.1809039309254672
2224	48-49	0.2328711751834902
2224	50-51	0.3168018376529318
2224	52-53	0.34946387781260313
2224	54-55	0.33039612815671404
2224	56-57	0.3999747115472658
2224	58-59	0.45489421332623436
2224	60-61	0.34947126445261034
2224	62-63	0.2706120398852718
2224	64-65	0.4427139557112909
2224	66-67	0.3072650795223808
2224	68-69	0.3029035146062995
2224	70-71	0.3350597339566832
2224	72-73	0.4766662161201509
2224	74-75	0.25030551440703874
2224	76-77	0.49594442790122173
2224	78-79	0.38391095161884436
2224	80-81	0.3062269465844878
2224	82-83	0.25190680913198094
2224	84-85	0.12376807593837214
2224	86-87	0.2807565998405295
2224	88-89	0.41095850706491177
2224	90-91	0.5530876970678449
2224	92-93	0.17679753730827485
2224	94-95	0.3670664133682209
2224	96-97	0.2544332934814264
2224	98-99	0.2793180585791504
2224	100	0.5481893903783543
2225	1	1.3659054809652016
2225	2	1.8678220153842027
2225	3	0.7026470860046228
2225	4	0.8535297843801359
2225	5	3.282198709288572
2225	6	-0.09998338302497345
2225	7	0.40471148757813324
2225	8	0.4908724630646546
2225	9	0.2782660454054806
2225	10-11	0.26305885802695883
2225	12-13	0.21082338947925194
2225	14-15	0.2606484538344418
2225	16-17	0.2172617030757138
2225	18-19	0.15603036109642687
2225	20-21	0.20637049476926705
2225	22-23	0.26235796698960456
2225	24-25	0.30033228228466413
2225	26-27	0.21223562777323934
2225	28-29	0.29519445068268624
2225	30-31	0.2824101644451531
2225	32-33	0.2544462650660506
2225	34-35	0.2457407431388745
2225	36-37	0.1711482735315215
2225	38-39	0.1848788128191643
2225	40-41	0.20673089218337992
2225	42-43	0.39342894051979016
2225	44-45	0.2737668445792778
2225	46-47	0.24308589585717755
2225	48-49	0.23552558852794903
2225	50-51	0.18923578216845272
2225	52-53	0.18443326708849384
2225	54-55	0.3439376234465499
2225	56-57	0.4168527564094404
2225	58-59	0.40115506934260026
2225	60-61	0.3688728794755818
2225	62-63	0.5301251716246114
2225	64-65	0.2948734005181137
2225	66-67	0.34237915116482753
2225	68-69	0.5529171602341805
2225	70-71	0.4710383190308818
2225	72-73	0.4878230653543483
2225	74-75	0.45012134681937965
2225	76-77	0.6040248171016174
2225	78-79	0.47367711781679134
2225	80-81	0.53133369216998
2225	82-83	0.44736344410079454
2225	84-85	0.47384303011422446
2225	86-87	0.4161947793395626
2225	88-89	0.49913103812566106
2225	90-91	0.6668861366273902
2225	92-93	0.595003369507701
2225	94-95	0.6572785302986688
2225	96-97	0.6489372023247597
2225	98-99	0.6384486772390119
2225	100	0.6217068074255181
2226	1	1.698159980030331
2226	2	1.4616664762754432
2226	3	0.9625995634119153
2226	4	1.0328238596372241
2226	5	3.1863783602701865
2226	6	-0.2187885014419244
2226	7	0.27693959602313356
2226	8	0.11814147210174042
2226	9	0.14826088414029215
2226	10-11	0.15498026072250326
2226	12-13	0.23902147494201387
2226	14-15	0.20494773904790264
2226	16-17	0.23257722192894192
2226	18-19	0.18256919718243125
2226	20-21	0.20919107750532362
2226	22-23	0.14622998708932755
2226	24-25	0.22266697572498373
2226	26-27	0.0753130564893496
2226	28-29	0.2247811112467204
2226	30-31	0.11053102569476891
2226	32-33	0.14072718396602113
2226	34-35	0.1383170422195832
2226	36-37	0.22675496564745146
2226	38-39	0.1392103315944837
2226	40-41	0.1427493656174761
2226	42-43	0.20740589011467847
2226	44-45	0.2636344045668153
2226	46-47	0.289933434031056
2226	48-49	0.12235584133255628
2226	50-51	0.14216577398833863
2226	52-53	0.15016942905484143
2226	54-55	0.3273021840573378
2226	56-57	0.38317735129881214
2226	58-59	0.42284937636970454
2226	60-61	0.2580989818439221
2226	62-63	0.24774422156229292
2226	64-65	0.25962001161190784
2226	66-67	0.22189920219319248
2226	68-69	0.23900794007213477
2226	70-71	0.3383865973107163
2226	72-73	0.34761924407045797
2226	74-75	0.3130947224815728
2226	76-77	0.5193421375285467
2226	78-79	0.3567147171468008
2226	80-81	0.290114757143499
2226	82-83	0.231875753231364
2226	84-85	0.13194451227377613
2226	86-87	0.2657828032566769
2226	88-89	0.2861691048910089
2226	90-91	0.3250218973784058
2226	92-93	0.16267680749461277
2226	94-95	0.4635668015669836
2226	96-97	0.5123952500031663
2226	98-99	0.3162357604425097
2226	100	0.4982844990740105
2227	1	1.2059566472247578
2227	2	1.8198104756421323
2227	3	0.6626866438425676
2227	4	-0.07758719957746862
2227	5	3.0808447947223705
2227	6	0.21435074808309196
2227	7	0.26814922236892613
2227	8	0.2424487868579135
2227	9	0.26134808331698167
2227	10-11	0.25767826974720265
2227	12-13	0.11841003178178511
2227	14-15	0.1478535536457528
2227	16-17	0.17204998950335693
2227	18-19	0.06406420984868078
2227	20-21	0.1059841427364816
2227	22-23	0.20648271824828868
2227	24-25	0.3165880689802165
2227	26-27	0.22489259654634708
2227	28-29	0.21012102179137315
2227	30-31	0.21293315916531697
2227	32-33	0.19071204384569285
2227	34-35	0.249292639907992
2227	36-37	0.0992149878133759
2227	38-39	0.16003551762994306
2227	40-41	0.15125326444584175
2227	42-43	0.4497247034459022
2227	44-45	0.29622781811969645
2227	46-47	0.36974898184612925
2227	48-49	0.22218019614572881
2227	50-51	0.30971198598960115
2227	52-53	0.308884102107406
2227	54-55	0.25712446081477225
2227	56-57	0.3886764804945102
2227	58-59	0.5327964354260715
2227	60-61	0.35888765347153395
2227	62-63	0.350789760669322
2227	64-65	0.23826749419583138
2227	66-67	0.15100177898229816
2227	68-69	0.3786336921303999
2227	70-71	0.4762891463924177
2227	72-73	0.39677515762333826
2227	74-75	0.27049834027764774
2227	76-77	0.4465160806887738
2227	78-79	0.39121410362558606
2227	80-81	0.284573077282829
2227	82-83	0.2852323080128727
2227	84-85	0.10907847759993672
2227	86-87	0.2444709563599048
2227	88-89	0.25017292455535056
2227	90-91	0.20626605903134987
2227	92-93	0.05430620109816431
2227	94-95	0.3079099380457677
2227	96-97	0.39198320123811925
2227	98-99	0.29647246088582335
2227	100	0.3706857419492451
2228	1	1.3001863854484341
2228	2	1.6381472545403994
2228	3	-0.09946980708604869
2228	4	-0.21374040202891997
2228	5	3.198995804679832
2228	6	0.29404445345942065
2228	7	0.1396204604139868
2228	8	0.2454805732605223
2228	9	0.26117086300029513
2228	10-11	0.09363880088849186
2228	12-13	0.18215797470712403
2228	14-15	0.1640351470497805
2228	16-17	0.1584752801813707
2228	18-19	0.13140316993532508
2228	20-21	0.042867479008606324
2228	22-23	0.1635740796355165
2228	24-25	0.23303354908859575
2228	26-27	0.1908194865707742
2228	28-29	0.1901847774697103
2228	30-31	0.24736085853221823
2228	32-33	0.09794360465190266
2228	34-35	0.22355354589950593
2228	36-37	0.15277692775205765
2228	38-39	0.11591802224590708
2228	40-41	0.16007138840168977
2228	42-43	0.4659528580915051
2228	44-45	0.2080925943569767
2228	46-47	0.2258639852368134
2228	48-49	0.149876736650441
2228	50-51	0.2792422400860488
2228	52-53	0.3313063398753826
2228	54-55	0.13212934503447116
2228	56-57	0.4113309294384919
2228	58-59	0.37478657297165086
2228	60-61	0.36921656327204033
2228	62-63	0.3126214432190366
2228	64-65	0.3779031700835631
2228	66-67	0.3355909204049041
2228	68-69	0.3003106921861374
2228	70-71	0.36993979962829115
2228	72-73	0.5281043873526414
2228	74-75	0.4229172835526711
2228	76-77	0.4159657575567337
2228	78-79	0.41279828863129353
2228	80-81	0.24005730725311025
2228	82-83	0.20337590982390452
2228	84-85	0.2446018388475295
2228	86-87	0.32362794301552356
2228	88-89	0.32285477471562274
2228	90-91	0.2715439279922478
2228	92-93	0.2503563995710252
2228	94-95	0.6489429172888705
2228	96-97	0.5313032127341444
2228	98-99	0.5388105333833124
2228	100	0.6149481970069886
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	27.0
13	55.0
14	166.0
15	640.0
16	2409.0
17	6280.0
18	10163.0
19	12947.0
20	15150.0
21	17497.0
22	20071.0
23	22689.0
24	25584.0
25	28891.0
26	32898.0
27	37037.0
28	41469.0
29	47119.0
30	53286.0
31	61828.0
32	71970.0
33	86710.0
34	105950.0
35	136125.0
36	190794.0
37	314431.0
38	775249.0
39	1463767.0
40	1697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.739044713771055	19.938894767687437	19.030373839129634	35.291686679411875
2	18.31855754185017	50.64200865166739	13.762531882717377	17.276901923765067
3	41.27386789301066	15.214188287194252	24.500579000412795	19.011364819382294
4	17.11814929753811	16.987082248201805	21.6704406124761	44.22432784178398
5	17.227585818076367	35.73399640905311	26.34612362782205	20.692294145048464
6	17.717412631503148	28.495835355671485	30.579846096694325	23.206905916131042
7	20.240341689788075	26.85127322874577	35.86179794629991	17.046587135166245
8	20.64174848356038	27.894953220841558	34.034171769843354	17.429126525754704
9	20.85551392880458	28.257452973137116	27.75439664919385	23.132636448864453
10-11	19.526952894848556	30.22398901001675	28.833118097942478	21.41593999719222
12-13	23.730322847504215	26.9726553832525	27.443921807452572	21.85309996179072
14-15	21.942761992453597	27.501472969235248	28.477330787164252	22.078434251146906
16-17	20.76834987533838	29.65884609083315	28.375820808791985	21.196983225036487
18-19	21.374855947655792	29.528588441929287	29.889622900338527	19.206932710076394
20-21	22.661370024664386	29.074082188752737	29.896279521136375	18.3682682654465
22-23	21.7212095568421	30.143635084326963	28.818800083396155	19.31635527543478
24-25	22.11897404866841	28.554237783426213	26.408210223062383	22.918577944842987
26-27	20.11873625240343	26.687551058514348	29.917868184394813	23.275844504687406
28-29	19.534347515613458	28.13030778396134	30.712708873139015	21.622635827286185
30-31	20.150134039277248	30.06548162026187	28.324185241052973	21.460199099407905
32-33	20.676636433234652	30.11661785609921	28.795648439992306	20.41109727067383
34-35	21.292855310741384	29.148435387098548	30.02738843601229	19.531320866147777
36-37	21.817193283985954	28.289047500362134	28.827940726210816	21.065818489441092
38-39	21.58982612650468	29.262292576873218	29.005361214570414	20.14252008205169
40-41	20.82467692229542	29.793400245698564	29.85166399165133	19.530258840354684
42-43	20.547793992460853	29.785246468082544	29.40012526172947	20.266834277727135
44-45	20.567144091976918	30.163618901900392	28.770822175004096	20.4984148311186
46-47	20.097157968921174	28.233433114930257	28.952977050232708	22.71643186591586
48-49	20.905640934896574	28.204158141214698	30.28809910633819	20.602101817550537
50-51	21.8559189081244	30.713466943946788	28.170302874850783	19.260311273078027
52-53	20.519010604809488	30.238185715276543	28.304993042560607	20.937810637353362
54-55	22.240790935043794	28.509860098171774	28.236424053941295	21.012924912843133
56-57	21.451061702425207	28.781178288575493	29.368575193698483	20.39918481530082
58-59	21.261443535177253	28.22915933911944	28.988376974808872	21.521020150894437
60-61	19.6078642068843	30.346802296413383	29.65647996876266	20.388853527939656
62-63	20.51978572248041	31.154456285169648	27.278764267093784	21.046993725256158
64-65	19.895910973655518	30.35809455176534	28.79421931067186	20.951775163907282
66-67	20.175198910156272	30.07237993591223	29.622088147056335	20.13033300687516
68-69	20.991004770159584	29.93596805268583	29.143606894863627	19.92942028229096
70-71	19.487724884234805	30.648756774890945	29.586265200330793	20.277253140543454
72-73	20.07600549164238	30.56785859718624	28.69383144766291	20.66230446350846
74-75	20.096617292309944	29.26858948577674	30.453244704916326	20.18154851699699
76-77	19.7535989711125	30.387934463126086	28.71599227329601	21.1424742924654
78-79	19.496460826833243	29.409578668000407	28.635163871490654	22.45879663367569
80-81	20.646974386340872	29.504006957506128	28.581470336959548	21.267548319193455
82-83	20.01722627403117	28.71458280012917	29.958114923139057	21.310076002700605
84-85	20.60293912834272	29.23025460667465	28.275190006751515	21.891616258231114
86-87	21.40674353365808	28.71271280602663	29.21218264874338	20.668361011571914
88-89	21.14359070685498	28.495695803872785	29.686044736399214	20.674668752873025
90-91	21.26396808841109	29.095740627910526	29.375835043075455	20.26445624060293
92-93	21.589947134987618	28.732654758060445	30.229724588943203	19.44767351800874
94-95	21.308652574353896	29.198939183047024	29.719258064489118	19.77315017810996
96-97	20.651419423210086	29.06404841442642	30.131131243163704	20.153400919199786
98-99	22.51524533624866	29.221490753716473	28.795606574452698	19.467657335582164
100	22.04910046306078	30.846138838968105	28.28310259373764	18.82165810423347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	12.0
2	25.0
3	29.5
4	31.5
5	40.0
6	53.5
7	64.0
8	75.0
9	98.0
10	122.0
11	129.0
12	137.5
13	166.0
14	201.5
15	253.0
16	344.0
17	477.5
18	679.5
19	1043.5
20	1672.0
21	2621.5
22	4037.5
23	6235.5
24	9453.5
25	14020.0
26	20186.0
27	27339.0
28	35191.5
29	44388.0
30	54967.0
31	66888.0
32	80205.0
33	94717.0
34	110591.5
35	128106.5
36	144522.5
37	156284.0
38	164022.0
39	168798.0
40	171398.0
41	172494.0
42	170925.5
43	167730.5
44	164607.0
45	159722.0
46	150261.0
47	138297.0
48	127496.5
49	119851.0
50	114141.0
51	106732.0
52	96011.0
53	81642.0
54	66501.0
55	53256.5
56	41660.5
57	30859.5
58	21479.5
59	15298.5
60	12115.5
61	10229.0
62	8374.0
63	6280.0
64	4663.5
65	4255.0
66	4990.5
67	4724.0
68	3203.0
69	2932.5
70	3821.5
71	3684.5
72	2177.5
73	927.5
74	417.5
75	233.5
76	165.5
77	122.5
78	88.0
79	74.5
80	63.0
81	48.0
82	38.5
83	33.5
84	38.0
85	33.0
86	29.0
87	25.5
88	16.0
89	13.0
90	13.5
91	17.0
92	19.0
93	15.5
94	12.0
95	15.5
96	23.0
97	26.5
98	65.0
99	126.5
100	115.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.324823836786916
2	0.006614755258242
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.4887949674272146E-4
30-31	0.010243102024366303
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.90817770749329E-4
40-41	0.001493204246058848
42-43	2.791035973941772E-5
44-45	0.0
46-47	0.002065366620716911
48-49	0.0
50-51	0.0
52-53	0.0012141006486646707
54-55	0.019774489875377454
56-57	0.011108323176288251
58-59	0.014708759582673136
60-61	2.0932769804563286E-4
62-63	0.01233637900482263
64-65	1.2559661882737973E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.6746215843650632E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3582899.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.25640571856998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.25423122250795	61.601643204383116
2	8.984943351639753	12.984394243489
3	2.61394614534117	5.66623059612791
4	1.1199012120348784	3.236801453660418
5	0.5852723690315828	2.114483887630733
6	0.34036999807433277	1.4756347605172724
7	0.2361084032012275	1.1942241202690123
8	0.15822769370403358	0.9146371545753818
9	0.11360466649022492	0.7387798386096472
>10	0.5522274108802343	6.807361820225362
>50	0.028348261367952272	1.4013671670017058
>100	0.012072570740901834	1.4790718140114745
>500	6.680955164507133E-4	0.31551436329786875
>1k	7.85994692347548E-5	0.06985557620097561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7910359739417717E-5	0.0
3	0.0	0.0	0.0	2.7910359739417717E-5	0.0
4	0.0	0.0	0.0	2.7910359739417717E-5	0.0
5	0.0	0.0	0.0	2.7910359739417717E-5	0.0
6	0.0	0.0	0.0	2.7910359739417717E-5	0.0
7	0.0	0.0	0.0	2.7910359739417717E-5	0.0
8	0.0	0.0	0.0	2.7910359739417717E-5	0.0
9	0.0	0.0	0.0	2.7910359739417717E-5	0.0
10-11	0.0	0.0	0.0	2.7910359739417717E-5	0.0
12-13	0.0	0.0	0.0	2.7910359739417717E-5	0.0
14-15	0.0	0.0	0.0	5.5820719478835434E-5	0.0
16-17	0.0	0.0	0.0	5.5820719478835434E-5	0.0
18-19	0.0	0.0	0.0	5.5820719478835434E-5	0.0
20-21	0.0	0.0	0.0	1.3955179869708858E-4	0.0
22-23	0.0	1.3955179869708859E-5	0.0	1.3955179869708858E-4	0.0
24-25	0.0	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
26-27	0.0	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
28-29	1.3955179869708859E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
30-31	2.7910359739417717E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
32-33	2.7910359739417717E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
34-35	2.7910359739417717E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
36-37	2.7910359739417717E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
38-39	2.7910359739417717E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
40-41	5.5820719478835434E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
42-43	5.5820719478835434E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
44-45	5.5820719478835434E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
46-47	5.5820719478835434E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
48-49	5.5820719478835434E-5	2.7910359739417717E-5	0.0	1.3955179869708858E-4	0.0
50-51	8.373107921825315E-5	2.7910359739417717E-5	0.0	1.674621584365063E-4	0.0
52-53	1.1164143895767087E-4	2.7910359739417717E-5	0.0	1.674621584365063E-4	0.0
54-55	1.674621584365063E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
56-57	1.9537251817592402E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
58-59	2.6514841752446833E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
60-61	3.4887949674272146E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
62-63	3.6283467661243034E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
64-65	4.047002162215569E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
66-67	5.442520149186455E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
68-69	6.000727343974809E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
70-71	6.419382740066075E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
72-73	7.256693532248606E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
74-75	7.95445252573405E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
76-77	9.070866915310758E-4	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
78-79	0.0011303695694464175	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
80-81	0.0013396972674920506	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
82-83	0.00146529388631943	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
84-85	0.001576935325277101	8.373107921825315E-5	0.0	1.674621584365063E-4	0.0
86-87	0.00159089050514681	1.3955179869708858E-4	0.0	1.674621584365063E-4	0.0
88	0.00159089050514681	1.3955179869708858E-4	0.0	1.674621584365063E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCTGC	45	1.031367E-9	82.30551	1
TCACCGG	13230	0.0	78.018135	8
CACCGGC	13240	0.0	75.477554	9
CTTCACC	14885	0.0	72.591644	6
CCTTCAC	16490	0.0	69.90977	5
GTGTGGC	5860	0.0	68.565605	7
TTCACCG	16510	0.0	64.622314	7
GGCGCGC	610	0.0	63.86728	6
CGGGGCA	1550	0.0	63.59424	94
CGCTGCC	75	1.2732926E-11	63.49283	1
AGGCGCG	630	0.0	61.83975	5
ACCTTCA	6815	0.0	61.43688	4
TGTGGCA	6600	0.0	60.45123	8
CGTAGTG	2510	0.0	60.216053	4
CGGGCCC	990	0.0	59.26592	7
GTGCAGC	40	8.0965565E-6	58.677345	2
GAGCTGC	55	3.891455E-7	57.72075	1
GCCTTCA	4955	0.0	57.122055	4
GGCCCGA	1015	0.0	56.41881	9
TGTGTGG	6710	0.0	56.24238	6
>>END_MODULE