FastQCFastQC Report
Tue 7 Mar 2017
1NF_4A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_4A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3582899
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTGC451.031367E-982.305511
TCACCGG132300.078.0181358
CACCGGC132400.075.4775549
CTTCACC148850.072.5916446
CCTTCAC164900.069.909775
GTGTGGC58600.068.5656057
TTCACCG165100.064.6223147
GGCGCGC6100.063.867286
CGGGGCA15500.063.5942494
CGCTGCC751.2732926E-1163.492831
AGGCGCG6300.061.839755
ACCTTCA68150.061.436884
TGTGGCA66000.060.451238
CGTAGTG25100.060.2160534
CGGGCCC9900.059.265927
GTGCAGC408.0965565E-658.6773452
GAGCTGC553.891455E-757.720751
GCCTTCA49550.057.1220554
GGCCCGA10150.056.418819
TGTGTGG67100.056.242386
Produced by FastQC (version 0.11.5)