FastQCFastQC Report
Tue 7 Mar 2017
1NF_5A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_5A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2765984
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA133890.48405919918553403No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG66960.24208382984138738No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG65160.2355762000069415No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA58740.21236565359741777No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA58280.21070259263972604No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG54130.19569889052142025No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT53680.19407198306280876No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50580.18286439834792972No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA43700.15799079098071428No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42630.154122366579127No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT40680.14707243425847727No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA40190.1453009128035448No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA37820.13673253352152437No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA37440.13535870055647467No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC34790.12577802330020707No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA34560.12494649282136121No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT33920.1226326688802249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA401.4551915E-1189.999753
GCTGCCA256.37785E-789.999753
GCTGCAT650.089.999753
TGCTGCC355.0749804E-1089.999752
GCAGCAA800.089.999753
AGCAGCC800.089.999752
GCAGCCG158.205546E-489.999743
CAGCTGC5950.089.243451
GCTGCCC1600.087.1872563
AGCTGCC2250.085.999762
CTGCAGC1000.085.4997561
AGCTGCG950.085.2629242
AGCAGCA1900.085.2629242
AGCTGCA1850.085.1348952
CAGCAGC5000.084.599761
GCTGCAA750.083.999763
GCAGCAT750.083.999763
GCTGCAG700.083.571193
CAGCCCT160300.083.515051
CAGCGTA60300.083.5072251
Produced by FastQC (version 0.11.5)