FastQCFastQC Report
Tue 7 Mar 2017
1NF_5A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_5A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2765984
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG108050.079.281668
CACCGGC107100.077.705899
CTTCACC121850.073.114816
CGGGCCC9850.071.480847
GGGCCCG10000.069.469858
CCTTCAC136700.069.086655
CGTAGTG20400.067.1873254
ACGGGCC10600.066.423246
GTGTGGC47100.066.0734947
TTCACCG133600.065.5257
AACGGGC10550.064.513445
GGCCCGA10800.064.323949
GCAGCTC301.3803277E-462.5854534
CGGGGCA10300.061.9778394
CAGCGTA11750.060.812811
CAGCTGC704.965841E-1060.4910471
GCGTAGT15000.060.0820353
TGTGGCA52600.059.7000968
GCTGCCA1500.059.4604762
ACCTTCA58250.058.7443734
Produced by FastQC (version 0.11.5)