##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_5A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2765984
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.771036998044819	12.0	12.0	12.0	2.0	27.0
2	14.663605429387879	12.0	12.0	12.0	12.0	22.0
3	13.706830191353239	12.0	12.0	12.0	12.0	22.0
4	13.77275428925113	12.0	12.0	12.0	12.0	22.0
5	22.440989535731227	22.0	22.0	22.0	22.0	27.0
6	26.901680559251247	27.0	22.0	32.0	22.0	32.0
7	34.99148332022166	37.0	37.0	37.0	27.0	41.0
8	37.56254699954881	41.0	37.0	41.0	27.0	41.0
9	38.53790007462082	41.0	37.0	41.0	32.0	41.0
10-11	38.904251976873326	41.0	41.0	41.0	37.0	41.0
12-13	39.07747532162153	41.0	41.0	41.0	37.0	41.0
14-15	39.03275055097933	41.0	41.0	41.0	37.0	41.0
16-17	38.94004665247521	41.0	41.0	41.0	37.0	41.0
18-19	38.87594541400095	41.0	41.0	41.0	37.0	41.0
20-21	38.89128335521825	41.0	41.0	41.0	37.0	41.0
22-23	38.88096008509088	41.0	41.0	41.0	37.0	41.0
24-25	38.896195711905776	41.0	41.0	41.0	37.0	41.0
26-27	38.86363135144672	41.0	41.0	41.0	37.0	41.0
28-29	38.812170099320895	41.0	41.0	41.0	37.0	41.0
30-31	38.6937617860407	41.0	41.0	41.0	32.0	41.0
32-33	38.731597326665664	41.0	41.0	41.0	32.0	41.0
34-35	38.705034989356406	41.0	41.0	41.0	32.0	41.0
36-37	38.6253262853292	41.0	41.0	41.0	32.0	41.0
38-39	38.52657806408135	41.0	41.0	41.0	32.0	41.0
40-41	38.58201547803603	41.0	41.0	41.0	32.0	41.0
42-43	38.32782799900505	41.0	41.0	41.0	32.0	41.0
44-45	38.42911347281836	41.0	41.0	41.0	32.0	41.0
46-47	38.4413246786677	41.0	41.0	41.0	32.0	41.0
48-49	38.3828068058239	41.0	41.0	41.0	32.0	41.0
50-51	38.26622044812985	41.0	41.0	41.0	32.0	41.0
52-53	38.24118415001678	41.0	41.0	41.0	32.0	41.0
54-55	38.10826020685586	41.0	37.0	41.0	32.0	41.0
56-57	37.8911125299351	41.0	37.0	41.0	29.5	41.0
58-59	37.881671766720274	41.0	37.0	41.0	29.5	41.0
60-61	37.88400366741095	41.0	37.0	41.0	29.5	41.0
62-63	37.87222955736548	41.0	37.0	41.0	29.5	41.0
64-65	37.86954769080371	41.0	37.0	41.0	29.5	41.0
66-67	37.72337168255493	41.0	37.0	41.0	27.0	41.0
68-69	37.73582565915059	41.0	37.0	41.0	27.0	41.0
70-71	37.730283146974095	41.0	37.0	41.0	27.0	41.0
72-73	37.55037086259357	41.0	37.0	41.0	27.0	41.0
74-75	37.50318729247891	41.0	37.0	41.0	27.0	41.0
76-77	36.416552120330415	39.0	34.5	41.0	24.5	41.0
78-79	36.94615984763469	41.0	37.0	41.0	27.0	41.0
80-81	37.59610558123258	41.0	37.0	41.0	27.0	41.0
82-83	37.78682667723313	41.0	37.0	41.0	27.0	41.0
84-85	37.69496895137499	41.0	37.0	41.0	27.0	41.0
86-87	37.55005253103417	41.0	37.0	41.0	27.0	41.0
88-89	37.453074927403776	41.0	37.0	41.0	27.0	41.0
90-91	37.307331495771486	41.0	37.0	41.0	27.0	41.0
92-93	37.1755937489154	41.0	37.0	41.0	27.0	41.0
94-95	37.018384596584795	41.0	37.0	41.0	27.0	41.0
96-97	36.96088769855501	41.0	37.0	41.0	27.0	41.0
98-99	36.831316630898804	41.0	37.0	41.0	27.0	41.0
100	36.69984931221583	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3388857799791793
1101	2	-0.5922008853787375
1101	3	-0.11798185177041454
1101	4	-0.0426770210850993
1101	5	-1.1280213005862727
1101	6	-1.07427495040222
1101	7	-0.5015788434358228
1101	8	-0.384999077942183
1101	9	-0.27425893835070525
1101	10-11	-0.15525341144544313
1101	12-13	-0.09941488291218548
1101	14-15	-0.019407705854774804
1101	16-17	-0.19959938344854322
1101	18-19	-0.24827582718875618
1101	20-21	-0.14685355422275137
1101	22-23	-0.05320221749006748
1101	24-25	-0.09005503772174706
1101	26-27	-0.030190343504727934
1101	28-29	-0.11906867477480176
1101	30-31	-0.23740588875334367
1101	32-33	-0.2096492655222022
1101	34-35	0.011939457054154445
1101	36-37	-0.03875508478969181
1101	38-39	-0.1635961644895545
1101	40-41	-0.06557391840856042
1101	42-43	-0.018878503727030704
1101	44-45	-0.19959909610386006
1101	46-47	-0.11077122931625638
1101	48-49	-0.19305227522674073
1101	50-51	-0.1318032365429218
1101	52-53	-0.2831825139607389
1101	54-55	-0.1328192180209129
1101	56-57	-0.021046396678869428
1101	58-59	-0.05132583819325731
1101	60-61	-0.24626257260347018
1101	62-63	-0.449471297535041
1101	64-65	-0.21967449467972955
1101	66-67	-0.3409512058290076
1101	68-69	-0.30553086997331036
1101	70-71	-0.22630655063337457
1101	72-73	-0.05642170642880018
1101	74-75	-0.1350195814151789
1101	76-77	-0.6019272481106057
1101	78-79	-0.26119624671024866
1101	80-81	-0.22626727552393788
1101	82-83	-0.17540422748780315
1101	84-85	-0.11178304662833938
1101	86-87	-0.2775526161923878
1101	88-89	-0.41428849156636716
1101	90-91	-0.3764687025181459
1101	92-93	-0.1991816984820005
1101	94-95	-0.18846694421570476
1101	96-97	-0.3242038131945293
1101	98-99	-0.5352791422689904
1101	100	-0.7058433543248128
1102	1	1.6727697462469013
1102	2	0.8423474460776923
1102	3	-0.007090807493657536
1102	4	-0.01597579591284415
1102	5	-0.8517986648769167
1102	6	-0.44523054451139643
1102	7	-0.23553441993593083
1102	8	-0.3115746078704831
1102	9	-0.25111692620379245
1102	10-11	-0.3405599812230804
1102	12-13	-0.6756738268541227
1102	14-15	-0.45263527294324746
1102	16-17	-0.44569415212820473
1102	18-19	-0.3026736094517233
1102	20-21	-0.3362246335834911
1102	22-23	-0.33117729142721686
1102	24-25	-0.347715278194606
1102	26-27	-0.3346650606485042
1102	28-29	-0.34110743653829445
1102	30-31	-0.3819975972275813
1102	32-33	-0.45498541854337304
1102	34-35	-0.24296705972854937
1102	36-37	-0.4831810164144983
1102	38-39	-0.3537819569859053
1102	40-41	-0.29693953560240516
1102	42-43	-0.3705103198010917
1102	44-45	-0.245890499438012
1102	46-47	-0.6147933932701477
1102	48-49	-0.571690205003442
1102	50-51	-0.4260820485509669
1102	52-53	-0.4371511379458042
1102	54-55	-0.5201625706375808
1102	56-57	-0.44437707747081134
1102	58-59	-0.2785129060896523
1102	60-61	-0.3310933619846992
1102	62-63	-0.6220143076971496
1102	64-65	-0.5757132748908234
1102	66-67	-0.38966209586705247
1102	68-69	-0.6158906233456634
1102	70-71	-0.583127257562218
1102	72-73	-0.2660934481422572
1102	74-75	-0.328952920170984
1102	76-77	-0.20244763815997402
1102	78-79	-0.5040512812701579
1102	80-81	-0.5300206914745687
1102	82-83	-0.5014651532051886
1102	84-85	-0.529722499925299
1102	86-87	-0.2737298540331139
1102	88-89	-0.4634406146755907
1102	90-91	-0.5477615523119113
1102	92-93	-0.46928702589756455
1102	94-95	-0.43673710429895607
1102	96-97	-0.2610318488520704
1102	98-99	-0.35541905893614967
1102	100	-0.7148717778850866
1103	1	1.4254136471529026
1103	2	0.5731361679656395
1103	3	0.20080907625779965
1103	4	0.9177867494093661
1103	5	2.7579311273791163
1103	6	-0.10996107105126995
1103	7	-0.5432463862539905
1103	8	-0.21634017098605796
1103	9	-0.20137185248186995
1103	10-11	-0.2622964727957111
1103	12-13	-0.3320068638608049
1103	14-15	-0.4150903731847535
1103	16-17	-0.38401004121179483
1103	18-19	-0.30903287337005736
1103	20-21	-0.347307528916069
1103	22-23	-0.3293688452641561
1103	24-25	-0.26580735268496625
1103	26-27	-0.2602993538467402
1103	28-29	-0.19501622193525492
1103	30-31	-0.11795147897600344
1103	32-33	-0.3809245745472509
1103	34-35	-0.4222104494754433
1103	36-37	-0.30219067568762625
1103	38-39	-0.25424886743829944
1103	40-41	-0.4146454969942255
1103	42-43	-0.26042706089784673
1103	44-45	-0.4062816959060669
1103	46-47	-0.5582888143179048
1103	48-49	-0.47448940302263054
1103	50-51	-0.36324904037559236
1103	52-53	-0.3244383937920716
1103	54-55	-0.32012100477162164
1103	56-57	-0.2770825676318438
1103	58-59	-0.4681530756491199
1103	60-61	-0.3814970264704627
1103	62-63	-0.31157120615884537
1103	64-65	-0.5951551599394236
1103	66-67	-0.4965193836646833
1103	68-69	-0.294161500742554
1103	70-71	-0.43355416130413715
1103	72-73	-0.23378870449442246
1103	74-75	-0.41515000404501734
1103	76-77	-0.5839405844185777
1103	78-79	-0.5491998949196102
1103	80-81	-0.17361621204697997
1103	82-83	-0.3932646398859063
1103	84-85	-0.5169625978507142
1103	86-87	-0.5794933986084985
1103	88-89	-0.4032467602568204
1103	90-91	-0.8759271126750079
1103	92-93	-0.4548789483920288
1103	94-95	-0.407021952425886
1103	96-97	-0.2611503098279755
1103	98-99	-0.43217628379267126
1103	100	-0.42083682806462974
1104	1	1.628058948717797
1104	2	-0.5916367502683784
1104	3	0.05455223484921312
1104	4	0.21891989608244522
1104	5	-1.0580146222220996
1104	6	-0.5031611568839658
1104	7	-0.208405293076396
1104	8	-0.15583329273663082
1104	9	-0.1919648995166341
1104	10-11	-0.2859738866463175
1104	12-13	-0.08404551959672801
1104	14-15	-0.07943748887016255
1104	16-17	-0.07756741855765625
1104	18-19	-0.11029916413089325
1104	20-21	-0.052390789214058486
1104	22-23	0.031687672462105354
1104	24-25	-0.06482699854753804
1104	26-27	-0.11403036142431233
1104	28-29	-0.17749523558316582
1104	30-31	-0.050079018085462224
1104	32-33	0.007623224180257182
1104	34-35	-0.10511733392964118
1104	36-37	-0.11237762032813237
1104	38-39	-0.10975237184239006
1104	40-41	-0.0839207536520803
1104	42-43	-0.05423857540537114
1104	44-45	-0.0340134118618991
1104	46-47	-0.31162116522884276
1104	48-49	-0.3637155902671694
1104	50-51	-0.4131245571168449
1104	52-53	-0.19743522160186444
1104	54-55	-0.44187649017877106
1104	56-57	-0.1709951682331834
1104	58-59	-0.03914757149838266
1104	60-61	-0.15504814733685635
1104	62-63	-0.22787114488671278
1104	64-65	-0.3476033306947741
1104	66-67	-0.7201068283064771
1104	68-69	-0.38063716381303436
1104	70-71	-0.31692614520274986
1104	72-73	-0.14193712266510516
1104	74-75	-0.22270069574794604
1104	76-77	-0.4198269233181122
1104	78-79	-0.33706827100124315
1104	80-81	-0.2979360784375302
1104	82-83	-0.2655600698120466
1104	84-85	-0.3423326220751832
1104	86-87	-0.3157499556316594
1104	88-89	-0.17482889981767613
1104	90-91	-0.36599611492164996
1104	92-93	-0.48111195313092736
1104	94-95	-0.308804014944144
1104	96-97	-0.10456071161287639
1104	98-99	-0.4096400311136321
1104	100	-0.5661153835934698
1105	1	1.4274373651970969
1105	2	0.30255149383320656
1105	3	-0.05108111590780062
1105	4	-0.23192990429554072
1105	5	-1.1884779670517247
1105	6	0.3345403513064156
1105	7	-0.1689715876766371
1105	8	-0.1367372732554557
1105	9	9.654216042775943E-4
1105	10-11	-0.18340952115587328
1105	12-13	-0.1325388715042095
1105	14-15	-0.18343693197935096
1105	16-17	-0.07733240729895385
1105	18-19	-0.12030527492382248
1105	20-21	-0.15032812565863907
1105	22-23	-0.15534919320965912
1105	24-25	-0.05857948392290524
1105	26-27	-0.17698745165313312
1105	28-29	-0.09942406193577824
1105	30-31	-0.16528663915293862
1105	32-33	-0.27359596654400775
1105	34-35	-0.29581525651014573
1105	36-37	-0.27483509279413454
1105	38-39	-0.1702048706398216
1105	40-41	-0.23410828746777668
1105	42-43	0.014805464820817349
1105	44-45	-0.10313998530771329
1105	46-47	-0.2524943666924315
1105	48-49	-0.2759153339973537
1105	50-51	-0.1839428114642203
1105	52-53	-0.18697734564011625
1105	54-55	-0.16762843867137178
1105	56-57	0.04088803142133912
1105	58-59	-0.07323145692893718
1105	60-61	-0.15208745565845305
1105	62-63	-0.2713272192899936
1105	64-65	-0.37453450382529496
1105	66-67	-0.23333656994415009
1105	68-69	-0.385832921772284
1105	70-71	-0.30051729063878696
1105	72-73	-0.3966382231913954
1105	74-75	-0.3134656453068061
1105	76-77	-0.44589741669170024
1105	78-79	-0.2709411548369829
1105	80-81	-0.5326831474661446
1105	82-83	-0.5446494866356488
1105	84-85	-0.37012639240107603
1105	86-87	-0.413889956982203
1105	88-89	-0.2460099668638236
1105	90-91	-0.34656112567181196
1105	92-93	-0.3157702364232833
1105	94-95	-0.17245980306552866
1105	96-97	-0.1705077552979617
1105	98-99	-0.4140347166412255
1105	100	-0.4466583021181805
1106	1	1.0054691750054552
1106	2	-0.7667310507379561
1106	3	-0.11043760714102113
1106	4	-0.11373484203502748
1106	5	-1.2096601528651476
1106	6	-0.8008740148826874
1106	7	-0.2563661604733696
1106	8	-0.17490257136731913
1106	9	-0.22455788630620077
1106	10-11	-0.12410248671525892
1106	12-13	-0.07238665133624522
1106	14-15	0.05433965075477687
1106	16-17	-0.11792525829874023
1106	18-19	-0.09894252594681774
1106	20-21	-0.16824899879371458
1106	22-23	-0.21507473386497367
1106	24-25	-0.17618773675203414
1106	26-27	-0.13848137705794272
1106	28-29	-0.14368839172868775
1106	30-31	-0.09830404394069348
1106	32-33	-0.099893619373006
1106	34-35	-0.10548650311488927
1106	36-37	-0.4208878402565688
1106	38-39	-0.20410128860026333
1106	40-41	-0.07390080579764202
1106	42-43	0.08560056991608178
1106	44-45	-0.08561593412752444
1106	46-47	-0.432071146653243
1106	48-49	-0.24423287896549795
1106	50-51	-0.29268587166706084
1106	52-53	-0.22012615704244354
1106	54-55	-0.18260533993058914
1106	56-57	-0.17151938512928666
1106	58-59	-0.1967474195466039
1106	60-61	-0.1351147684849323
1106	62-63	-0.31783136632128617
1106	64-65	-0.5372482689267173
1106	66-67	-0.4058703023123442
1106	68-69	-0.3657668081320864
1106	70-71	-0.5074309999616204
1106	72-73	-0.3383732008365925
1106	74-75	-0.44804099744250436
1106	76-77	-0.3472025922908344
1106	78-79	-0.3098183638792591
1106	80-81	-0.4465482633635105
1106	82-83	-0.31772320711419155
1106	84-85	-0.4618764989875572
1106	86-87	-0.4275678042756397
1106	88-89	-0.5503348758066124
1106	90-91	-0.49839556401278884
1106	92-93	-0.37261068630829897
1106	94-95	-0.5112616843251914
1106	96-97	-0.40740604210799347
1106	98-99	-0.5408143271652293
1106	100	-0.5214666633018865
1107	1	1.3768101639781136
1107	2	-0.6857565665545433
1107	3	-0.09142148200351485
1107	4	-0.28929358744030154
1107	5	-1.1965836322459005
1107	6	-0.3982640768611674
1107	7	-0.5678650470071318
1107	8	-0.26322123735427994
1107	9	-0.27322977597913933
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2221	86-87	0.32588146251857353
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2222	2	1.6881349945854733
2222	3	0.5971112885210097
2222	4	0.8239062424081975
2222	5	3.2223449041239824
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2222	7	0.3201302738266065
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2222	26-27	0.1981440627335047
2222	28-29	0.15386780520641707
2222	30-31	0.0905268866694442
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2222	36-37	0.2257216412713703
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2222	44-45	0.3502156759746953
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2222	54-55	0.39164787213954355
2222	56-57	0.375061185317378
2222	58-59	0.48897287472573225
2222	60-61	0.5100094674948323
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2222	64-65	0.528058841892566
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2222	68-69	0.45964668566591627
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2222	84-85	0.3165153262000402
2222	86-87	0.369866541435556
2222	88-89	0.4048901896108248
2222	90-91	0.5210518749880961
2222	92-93	0.546183149914512
2222	94-95	0.747640151936821
2222	96-97	0.6586086551750157
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2223	1	1.3652016756583603
2223	2	-0.9062917508095314
2223	3	-0.09268540142579695
2223	4	0.18491919461758677
2223	5	-1.0392028320788995
2223	6	0.09512586918985733
2223	7	0.33187026380288387
2223	8	0.3754685593096241
2223	9	0.3128873330865076
2223	10-11	0.1602132612480247
2223	12-13	0.2997146738187766
2223	14-15	0.18690139352217727
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2223	18-19	0.1478057252093663
2223	20-21	0.20773955955720425
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2223	24-25	0.19293814418265498
2223	26-27	0.13310978588330613
2223	28-29	0.156795654243723
2223	30-31	0.20663148606595882
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2223	34-35	0.26171631717866717
2223	36-37	0.1888282970406152
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2223	40-41	0.270541970651891
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2223	46-47	0.31882981482823425
2223	48-49	0.32587026064957314
2223	50-51	0.290040579265991
2223	52-53	0.32248198601464395
2223	54-55	0.24941240673926046
2223	56-57	0.2707559875156065
2223	58-59	0.3567274570850927
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2223	62-63	0.21031792023639895
2223	64-65	0.19257636474578277
2223	66-67	0.37694978609210494
2223	68-69	0.42404482929111964
2223	70-71	0.4076591401477856
2223	72-73	0.4649489511417002
2223	74-75	0.36872605284851545
2223	76-77	0.5047322401972494
2223	78-79	0.46427810209180365
2223	80-81	0.2988232678493077
2223	82-83	0.36480050102736783
2223	84-85	0.46158142162558846
2223	86-87	0.629228914977368
2223	88-89	0.5357119523726084
2223	90-91	0.5001914073042855
2223	92-93	0.487036659767071
2223	94-95	0.4955513331557597
2223	96-97	0.6244369667174681
2223	98-99	0.571704421495582
2223	100	0.6291651087665002
2224	1	1.2302543632214942
2224	2	-1.0265510180047315
2224	3	-0.03158804358401035
2224	4	-0.3486212986635042
2224	5	-1.0713537562475821
2224	6	0.597849048401546
2224	7	0.45787449691888327
2224	8	0.18626252683235123
2224	9	0.23532686546684545
2224	10-11	0.21510833570699361
2224	12-13	0.22396410561956515
2224	14-15	0.22564352418631017
2224	16-17	0.2557108573586575
2224	18-19	0.21660916526976592
2224	20-21	0.21484906449051522
2224	22-23	0.37922508201063465
2224	24-25	0.3752562757956994
2224	26-27	0.30250505229284386
2224	28-29	0.3029453712282191
2224	30-31	0.267813659278076
2224	32-33	0.25495072732639557
2224	34-35	0.16672350097676514
2224	36-37	0.2341559745279369
2224	38-39	0.1723094792666231
2224	40-41	0.20477060036711237
2224	42-43	0.5024078871555773
2224	44-45	0.5224548514698526
2224	46-47	0.46416708984430244
2224	48-49	0.3551133089777565
2224	50-51	0.6329653831065372
2224	52-53	0.39022061055166546
2224	54-55	0.44951243148238973
2224	56-57	0.6394377207081376
2224	58-59	0.47141951989693354
2224	60-61	0.5224053488091229
2224	62-63	0.4512161309509466
2224	64-65	0.37178916969848785
2224	66-67	0.5286121035796327
2224	68-69	0.5101879109077387
2224	70-71	0.5962497829079965
2224	72-73	0.5772449797416925
2224	74-75	0.412642479098686
2224	76-77	0.4845452254942231
2224	78-79	0.42874205619712313
2224	80-81	0.5244265729703699
2224	82-83	0.4545208940860235
2224	84-85	0.2986637696976615
2224	86-87	0.33305770718099126
2224	88-89	0.4139398786942081
2224	90-91	0.5117270150925037
2224	92-93	0.32356276568297915
2224	94-95	0.4392463625924279
2224	96-97	0.4670952876792427
2224	98-99	0.43534753180912844
2224	100	0.5447840186402857
2225	1	1.555520903675859
2225	2	1.6393517320949105
2225	3	0.8544928983583304
2225	4	1.0608981812089535
2225	5	3.234447442769291
2225	6	0.07478001359352149
2225	7	0.4244552229717655
2225	8	0.6025327041938482
2225	9	0.3979373012314298
2225	10-11	0.4791608715778608
2225	12-13	0.4418079926263374
2225	14-15	0.29645526857319027
2225	16-17	0.4027481896291505
2225	18-19	0.3124350255062467
2225	20-21	0.35318023892920536
2225	22-23	0.4163383492485764
2225	24-25	0.4579315614340729
2225	26-27	0.3548935370809403
2225	28-29	0.3197421943317096
2225	30-31	0.34755575440229336
2225	32-33	0.36547679182752546
2225	34-35	0.35492410380657446
2225	36-37	0.40931809921239903
2225	38-39	0.4726231417483504
2225	40-41	0.3822888264199946
2225	42-43	0.5429795375475024
2225	44-45	0.5112078407086003
2225	46-47	0.48682614225562304
2225	48-49	0.5567675620002106
2225	50-51	0.3616712088007006
2225	52-53	0.5308331553902548
2225	54-55	0.5188025703159269
2225	56-57	0.6323112925162917
2225	58-59	0.7157897869355949
2225	60-61	0.5797468361214655
2225	62-63	0.6444756051770639
2225	64-65	0.6348608616076135
2225	66-67	0.7009292618100105
2225	68-69	0.6684016746914523
2225	70-71	0.6778954418690759
2225	72-73	0.7585194748924877
2225	74-75	0.6584585827920506
2225	76-77	0.8772606167523591
2225	78-79	0.7618470449044423
2225	80-81	0.6467923063302692
2225	82-83	0.47822202833605587
2225	84-85	0.5500846960295362
2225	86-87	0.5449916200944998
2225	88-89	0.5593850261633122
2225	90-91	0.7222431154645008
2225	92-93	0.6413136541511548
2225	94-95	0.6762971464372498
2225	96-97	0.8445645234305346
2225	98-99	0.8842056010930435
2225	100	0.919825535845014
2226	1	1.2623056452727752
2226	2	1.5095066743029601
2226	3	0.9439728538518875
2226	4	1.0656415218493152
2226	5	3.1053289360601113
2226	6	-0.3252278746753774
2226	7	0.05122385589324807
2226	8	0.18345803965286223
2226	9	0.09950955777453885
2226	10-11	-0.0028403822417288893
2226	12-13	0.10477340049135364
2226	14-15	0.036540960083748075
2226	16-17	0.017930408640708606
2226	18-19	0.07918929347489723
2226	20-21	0.13251733372128882
2226	22-23	0.20935328713883905
2226	24-25	0.21299666041108622
2226	26-27	0.13563806511335486
2226	28-29	0.13347421738206577
2226	30-31	0.1702575695344848
2226	32-33	0.12053566322383347
2226	34-35	0.13687574456650964
2226	36-37	0.1574332181176743
2226	38-39	0.1675017869589297
2226	40-41	0.22820810036711237
2226	42-43	0.3263261563863509
2226	44-45	0.2696503642903778
2226	46-47	0.21496837189557993
2226	48-49	0.3098408730803257
2226	50-51	0.27258878054243496
2226	52-53	0.33773663619268746
2226	54-55	0.31690025199521443
2226	56-57	0.20634477199018875
2226	58-59	0.28351887887128413
2226	60-61	0.22112329752707893
2226	62-63	0.2885558745406911
2226	64-65	0.38420904149335655
2226	66-67	0.30465377024629703
2226	68-69	0.43887380834363654
2226	70-71	0.41195491111312066
2226	72-73	0.37912799256220353
2226	74-75	0.4503027355089415
2226	76-77	0.513591699853194
2226	78-79	0.35702731260737863
2226	80-81	0.3092823422011435
2226	82-83	0.26141191972704547
2226	84-85	0.11937691072330381
2226	86-87	0.33566187384765556
2226	88-89	0.17315462228394551
2226	90-91	0.24550105355404384
2226	92-93	0.26707238106759235
2226	94-95	0.5019466831052526
2226	96-97	0.5287940056279581
2226	98-99	0.5266936856552817
2226	100	0.6974282494095192
2227	1	1.3167096667675047
2227	2	1.9164683520057881
2227	3	0.5624533317073652
2227	4	0.10159703941434728
2227	5	3.222149799562388
2227	6	0.33755454018115216
2227	7	0.43152662834203426
2227	8	0.5078103098015916
2227	9	0.32933343043580265
2227	10-11	0.3223681251432211
2227	12-13	0.28149541557067437
2227	14-15	0.27595088644057597
2227	16-17	0.2959309617380512
2227	18-19	0.21995804721377965
2227	20-21	0.23540327060889155
2227	22-23	0.2970055178358706
2227	24-25	0.3405597315582227
2227	26-27	0.2546701898838677
2227	28-29	0.27923003328444906
2227	30-31	0.38328401119754574
2227	32-33	0.332690718714062
2227	34-35	0.3452013422726381
2227	36-37	0.3455701180908619
2227	38-39	0.364760441836502
2227	40-41	0.3903194495636413
2227	42-43	0.5258331390791753
2227	44-45	0.2963482699982336
2227	46-47	0.40904252690724263
2227	48-49	0.33936341083018107
2227	50-51	0.47869441439972604
2227	52-53	0.5697500136706708
2227	54-55	0.47586030005923874
2227	56-57	0.5872625268017444
2227	58-59	0.6175841959288633
2227	60-61	0.5014666175551312
2227	62-63	0.5711530017865982
2227	64-65	0.5259611868406395
2227	66-67	0.4724688552350571
2227	68-69	0.4023197466236468
2227	70-71	0.5758125781511012
2227	72-73	0.5475965768908608
2227	74-75	0.4925042885650299
2227	76-77	0.5987778770609467
2227	78-79	0.6011659634108568
2227	80-81	0.4715874043955779
2227	82-83	0.4401740453226921
2227	84-85	0.4172307898824954
2227	86-87	0.40753874329089257
2227	88-89	0.4513580744365129
2227	90-91	0.40731619269218555
2227	92-93	0.3940847722531018
2227	94-95	0.5482226554735874
2227	96-97	0.5844229959223242
2227	98-99	0.7648125680634337
2227	100	0.581116970349342
2228	1	1.4977233433564034
2228	2	1.6369027848296032
2228	3	-0.08919590401266753
2228	4	-0.029135060344302133
2228	5	3.0873951736573204
2228	6	0.040692110575360374
2228	7	0.3438355583500794
2228	8	0.4148899558192909
2228	9	0.25210092842453946
2228	10-11	0.27389668507886
2228	12-13	0.1849547880566007
2228	14-15	0.27876665474307316
2228	16-17	0.26375075256669334
2228	18-19	0.15640213410034676
2228	20-21	0.14509755862175666
2228	22-23	0.28433456047464745
2228	24-25	0.3202459745188051
2228	26-27	0.2504465374301219
2228	28-29	0.3144271370656213
2228	30-31	0.1648608414469379
2228	32-33	0.18888540897955153
2228	34-35	0.1867138121036902
2228	36-37	0.2645162162405015
2228	38-39	0.16323188190086313
2228	40-41	0.19743932084280402
2228	42-43	0.3776030505835948
2228	44-45	0.23500474626532508
2228	46-47	0.3225582147592121
2228	48-49	0.3528388734206871
2228	50-51	0.16393174344236172
2228	52-53	0.23748935397638604
2228	54-55	0.3368820217004327
2228	56-57	0.41811530234479477
2228	58-59	0.3945351211098753
2228	60-61	0.36248833919631807
2228	62-63	0.3513219478847489
2228	64-65	0.3246337532123178
2228	66-67	0.45919451895186825
2228	68-69	0.3502280330275056
2228	70-71	0.42657685811892776
2228	72-73	0.4902755793043738
2228	74-75	0.5128180456044475
2228	76-77	0.4843370081952969
2228	78-79	0.5842713535958879
2228	80-81	0.3261730842024946
2228	82-83	0.37420461652140347
2228	84-85	0.4049620851359137
2228	86-87	0.3787776813439976
2228	88-89	0.48032026269073924
2228	90-91	0.2900595555240457
2228	92-93	0.40514671915575917
2228	94-95	0.5472170239369802
2228	96-97	0.22782605304219317
2228	98-99	0.47116815937478407
2228	100	0.6249625053453229
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	50.0
14	140.0
15	526.0
16	1740.0
17	4474.0
18	7132.0
19	9322.0
20	10973.0
21	12920.0
22	14762.0
23	16849.0
24	19181.0
25	22068.0
26	24783.0
27	28073.0
28	31762.0
29	35592.0
30	41446.0
31	48108.0
32	56157.0
33	67063.0
34	83364.0
35	107669.0
36	152209.0
37	250179.0
38	606427.0
39	1111692.0
40	1304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.795616212992716	19.943406078506744	18.861216492130698	37.39976121636984
2	18.9892758709084	50.519574852873596	14.340200859130695	16.150948417087307
3	41.006853257285655	15.73418356722237	23.227285479597857	20.03167769589412
4	17.037987204553605	18.697143584344666	21.024886622626884	43.239982588474845
5	17.07005535823779	35.08538010342793	27.09166068928815	20.752903849046124
6	17.35935565787799	28.639030449923062	30.385931371981904	23.61568252021704
7	20.03344921734905	26.491331837060518	36.220057672061735	17.255161273528696
8	20.819570901350115	28.113503187292476	33.99969052604787	17.06723538530953
9	20.68381451230376	28.29329453821859	27.827854391059386	23.19503655841827
10-11	19.526468699746637	30.351856699098768	28.786880184411768	21.33479441674283
12-13	24.102507462082208	26.76515843909437	27.390234361442438	21.742099737380983
14-15	21.89217291206312	27.572881838795887	28.429177464511724	22.10576778462927
16-17	20.640520697155154	29.853119179286647	28.40271310318498	21.10364702037322
18-19	21.365434507213347	29.574990310862248	29.794803585270195	19.26477159665421
20-21	22.615857503152583	28.875853945648277	30.11035132524266	18.397937225956476
22-23	21.729572549949676	30.274253213323	28.751305141316795	19.24486909541053
24-25	22.2012853291993	28.49096379443988	26.568682248342725	22.739068628018096
26-27	20.023958923840485	26.691676452213752	30.141443334451683	23.14292128949408
28-29	19.270564629109085	28.307197281248026	30.83115357152541	21.59108451811748
30-31	20.027967653700067	30.089254238084823	28.404234740273242	21.478543367941874
32-33	20.592743848120595	30.110640553235303	28.77955910084802	20.517056497796084
34-35	21.346706994689775	29.365914625681132	29.859572578872474	19.42780580075662
36-37	21.60276415192568	28.294126068697434	28.977933350301377	21.125176429075513
38-39	21.428233132547327	29.33513571945829	29.168014901139315	20.068616246855072
40-41	20.816389172898404	29.926827980858643	29.906491372939765	19.350291473303187
42-43	20.281422655678888	29.858834945419023	29.572734023576437	20.28700837532565
44-45	20.55174578016359	30.042183902726844	29.02214907967653	20.383921237433043
46-47	19.82411664239509	28.383227449488853	29.018837827792808	22.77381808032325
48-49	20.81980589909414	28.046402292999527	30.36346197230353	20.77032983560281
50-51	21.854735963765517	30.574743020928537	28.402243107696933	19.16827790760901
52-53	20.30919642624724	30.502660212932724	28.356575548890362	20.83156781192967
54-55	22.20175139479861	28.481825581559335	28.369963257375918	20.94645976626614
56-57	21.460284264157615	28.725439469032242	29.525228453027786	20.289047813782354
58-59	21.042233042971407	28.19728218923286	29.213274724591283	21.54721004320445
60-61	19.604830694665434	30.331954797921455	29.765229598633976	20.297984908779135
62-63	20.470801311736693	31.263540007734175	27.348560273339345	20.917098407189783
64-65	19.877786512879073	30.294060386203153	28.88380047646774	20.944352624450037
66-67	19.990679627937112	30.336111850249313	29.638493931996713	20.03471458981686
68-69	20.719841474137233	30.08291081944075	29.325784241702046	19.87146346471997
70-71	19.509115020188116	31.01445995349214	29.25058496361512	20.225840062704627
72-73	19.958991085993265	30.897394923470273	28.626611722988997	20.51700226754746
74-75	19.87417497715099	29.294963383736132	30.585299842659975	20.245561796452908
76-77	19.4876217649849	30.55364745421521	28.80412540347305	21.154605377326842
78-79	19.388741945000405	29.658450663489017	28.740965240579847	22.211842150930735
80-81	20.3683072735381	29.852383162052774	28.60298805648706	21.17632150792206
82-83	19.927067546305402	28.806149999421542	29.98092179853535	21.285860655737704
84-85	20.488929075511646	29.294294544003147	28.27530817242616	21.94146820805905
86-87	21.23672805048764	28.79429165172322	29.44436771868528	20.524612579103856
88-89	20.840467623818505	28.03875582794405	29.798798546918565	21.321978001318882
90-91	20.835930359683932	28.00856765621204	29.881228524821545	21.274273459282483
92-93	21.36886908962597	28.13429144926363	30.485733106192946	20.011106354917455
94-95	21.17891860545831	28.58438443606326	29.835403964737324	20.40129299374111
96-97	20.658977058435624	28.35882275530155	30.25742375950114	20.724776426761686
98-99	22.225508173583073	28.55032783992966	29.136556827515996	20.087607158971274
100	21.675974987563194	30.27855547971355	28.384184434906345	19.661285097816908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	13.5
2	20.0
3	14.0
4	17.5
5	27.0
6	40.0
7	54.5
8	58.5
9	68.5
10	87.5
11	111.0
12	128.0
13	137.0
14	162.5
15	204.0
16	283.0
17	406.5
18	550.5
19	861.0
20	1350.5
21	2053.5
22	3115.5
23	4885.0
24	7549.0
25	11243.5
26	15985.5
27	21229.5
28	27372.5
29	34432.5
30	42235.0
31	51244.0
32	61367.0
33	72443.0
34	84818.0
35	98887.0
36	112035.0
37	120975.0
38	127024.5
39	131267.5
40	133645.5
41	134036.0
42	131993.5
43	129880.5
44	128051.5
45	123761.0
46	115190.0
47	105100.5
48	98315.0
49	94201.5
50	89554.5
51	82685.5
52	72509.5
53	61605.0
54	52059.5
55	42368.0
56	32616.5
57	23983.0
58	16970.5
59	12152.5
60	9489.0
61	7914.5
62	6430.5
63	4757.0
64	3621.5
65	3168.0
66	2747.0
67	2093.0
68	1675.5
69	1781.0
70	2031.5
71	1831.5
72	1117.0
73	503.5
74	251.0
75	143.5
76	93.5
77	81.5
78	74.0
79	66.5
80	58.0
81	47.5
82	36.5
83	26.5
84	25.0
85	25.5
86	26.0
87	22.0
88	16.5
89	18.0
90	16.5
91	15.0
92	16.0
93	15.5
94	13.0
95	11.5
96	16.5
97	21.0
98	46.5
99	89.5
100	83.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.305813771880098
2	0.005965327348242073
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.2538149172229485E-4
30-31	0.01044836123419369
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.592234806853546E-4
40-41	0.0013015259668891794
42-43	7.230699816050997E-5
44-45	0.0
46-47	0.002169209944815299
48-49	0.0
50-51	0.0
52-53	0.0014461399632101992
54-55	0.019197508011615395
56-57	0.01146065920844083
58-59	0.01491331837060518
60-61	1.807674954012749E-4
62-63	0.012201805939586056
64-65	1.6269074586114742E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.9884424494140242E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2765984.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.36339781048679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0758027001827	64.00889158050659
2	8.66417013651551	12.885942611188849
3	2.3905775821345143	5.333144152113015
4	1.0270305790197873	3.054939340447321
5	0.535584297824485	1.9913934100086204
6	0.31834525391151275	1.4203940854621349
7	0.2133124281415456	1.1103845866267443
8	0.1470642267320157	0.8748956476931605
9	0.11481532246625933	0.7684251749367986
>10	0.48191427565602885	6.04551400627881
>50	0.021691741417326545	1.0982806231623057
>100	0.009246793895181585	1.1799292711776226
>500	4.4466210318329954E-4	0.2278655103980213
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.6153499080254985E-5	0.0
3	0.0	0.0	0.0	3.6153499080254985E-5	0.0
4	0.0	0.0	0.0	3.6153499080254985E-5	0.0
5	0.0	0.0	0.0	3.6153499080254985E-5	0.0
6	0.0	0.0	0.0	3.6153499080254985E-5	0.0
7	0.0	0.0	0.0	3.6153499080254985E-5	0.0
8	0.0	0.0	0.0	3.6153499080254985E-5	0.0
9	0.0	0.0	0.0	3.6153499080254985E-5	0.0
10-11	0.0	0.0	0.0	3.6153499080254985E-5	0.0
12-13	0.0	0.0	0.0	3.6153499080254985E-5	0.0
14-15	0.0	0.0	0.0	3.6153499080254985E-5	0.0
16-17	0.0	0.0	0.0	3.6153499080254985E-5	0.0
18-19	0.0	0.0	0.0	3.6153499080254985E-5	0.0
20-21	0.0	0.0	0.0	3.6153499080254985E-5	0.0
22-23	0.0	0.0	0.0	3.6153499080254985E-5	0.0
24-25	0.0	0.0	1.8076749540127493E-5	3.6153499080254985E-5	0.0
26-27	0.0	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
28-29	0.0	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
30-31	0.0	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
32-33	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
34-35	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
36-37	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
38-39	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
40-41	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
42-43	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
44-45	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
46-47	3.6153499080254985E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
48-49	9.038374770063747E-5	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
50-51	1.4461399632101994E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
52-53	1.4461399632101994E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
54-55	1.4461399632101994E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
56-57	1.6269074586114742E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
58-59	2.169209944815299E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
60-61	3.6153499080254987E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
62-63	4.6999548804331476E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
64-65	5.061489871235697E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
66-67	5.603792357439523E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
68-69	5.965327348242072E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
70-71	6.326862339044621E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
72-73	6.507629834445897E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
74-75	6.688397329847173E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
76-77	7.773002302254822E-4	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
78-79	0.0011930654696484144	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
80-81	0.0014099864641299443	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
82-83	0.0015546004604509642	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
84-85	0.0017534447053923667	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
86-87	0.0018438284530930041	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
88	0.0018438284530930041	0.0	3.6153499080254985E-5	3.6153499080254985E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10805	0.0	79.28166	8
CACCGGC	10710	0.0	77.70589	9
CTTCACC	12185	0.0	73.11481	6
CGGGCCC	985	0.0	71.48084	7
GGGCCCG	1000	0.0	69.46985	8
CCTTCAC	13670	0.0	69.08665	5
CGTAGTG	2040	0.0	67.187325	4
ACGGGCC	1060	0.0	66.42324	6
GTGTGGC	4710	0.0	66.073494	7
TTCACCG	13360	0.0	65.525	7
AACGGGC	1055	0.0	64.51344	5
GGCCCGA	1080	0.0	64.32394	9
GCAGCTC	30	1.3803277E-4	62.585453	4
CGGGGCA	1030	0.0	61.97783	94
CAGCGTA	1175	0.0	60.81281	1
CAGCTGC	70	4.965841E-10	60.491047	1
GCGTAGT	1500	0.0	60.082035	3
TGTGGCA	5260	0.0	59.700096	8
GCTGCCA	150	0.0	59.460476	2
ACCTTCA	5825	0.0	58.744373	4
>>END_MODULE