FastQCFastQC Report
Tue 7 Mar 2017
1NF_6A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_6A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2423888
Sequences flagged as poor quality0
Sequence length96
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA146400.6039883030899118No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG56100.23144633745453583No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA54570.2251341646148667No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT52780.2177493349527701No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA50320.20760035117134124No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG49340.20355726007142244No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC48630.2006280818255629No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG46680.19258315565735712No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG46630.192376875499198No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG45550.1879212240829609No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42140.17385291729650876No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG42070.17356412507508598No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT41080.16947977794353536No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA38280.15792808908662448No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC38010.1568141762325652No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA36640.15116209989900523No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA32650.13470094327790721No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA32620.13457717518301177No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC32370.13354577439221615No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA31480.12987398757698376No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA31000.12789369805865616No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA29790.12290171823120541No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA29600.12211785363020074No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG28710.11844606681496835No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA27630.11399041539873128No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT26910.11101998112123992No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC26250.10829708303353949No Hit
CAGCTCTGTTATAAAACAAGTTCTTGGATTTTTTTCATCATTTGAACATC24980.10305756701629779No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC24440.10082974130817925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC202.2812275E-589.999713
GCTGCCC1650.089.999713
GCTGCAT202.2812275E-589.999713
TGCTGCC301.7940692E-889.999712
AGCTGCC1800.089.999712
GCAGCTT158.205437E-489.999713
GCAGCTA158.205437E-489.999713
CAGCTGC4500.087.999721
AGCTGCG800.084.374732
AGCTGCA750.083.9997252
CAGCCCT150000.083.9397351
CAGCGTA50700.083.875471
AGCTGCT1250.082.799732
AGCCCTT127400.081.7344362
CAGCTAG39650.081.033791
GCTGCAA501.8189894E-1280.999743
CAGCGTG37400.080.013111
CAGCTCT59200.079.66191
AGCAGCA1300.079.615132
GCCCTTC116250.079.277173
Produced by FastQC (version 0.11.5)