FastQCFastQC Report
Tue 7 Mar 2017
1NF_6A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_6A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2423888
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG94000.079.194738
CACCGGC94700.075.883299
CGGGGCA9100.075.8222994
CTTCACC104050.074.7483146
AGGCTGC452.6593625E-973.02312
GTGTGGC43150.071.249447
CCTTCAC116100.070.7096255
CAGCTGC850.068.48451
CGTAGTG17900.067.390874
TTCACCG119300.064.4064257
CGGGCCC6800.064.1940847
TGTGGCA47200.064.041998
GCAGCCG250.00327367163.5038151
ACCTTCA46950.062.083614
GCCTTCA32250.061.564684
GCAGCCA850.060.7503052
CGCTGCA351.6348234E-460.479821
CAGCGTA8700.060.219131
GCGTAGT12700.059.8731733
TGTGTGG48900.058.9359746
Produced by FastQC (version 0.11.5)