##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_6A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2423888
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.801524657904986	12.0	12.0	12.0	2.0	32.0
2	14.656440809146297	12.0	12.0	12.0	12.0	22.0
3	13.72896396203125	12.0	12.0	12.0	12.0	22.0
4	13.772247727617778	12.0	12.0	12.0	12.0	22.0
5	22.431956839589947	22.0	22.0	22.0	22.0	27.0
6	26.82630756866654	27.0	22.0	32.0	22.0	32.0
7	34.94869977490709	37.0	37.0	37.0	27.0	41.0
8	37.55174042695042	41.0	37.0	41.0	27.0	41.0
9	38.51262145775713	41.0	37.0	41.0	32.0	41.0
10-11	38.87428111364882	41.0	41.0	41.0	37.0	41.0
12-13	39.053113633963285	41.0	41.0	41.0	37.0	41.0
14-15	39.006382926933924	41.0	41.0	41.0	37.0	41.0
16-17	38.91169992177856	41.0	41.0	41.0	37.0	41.0
18-19	38.850200999386104	41.0	41.0	41.0	37.0	41.0
20-21	38.87215477777851	41.0	41.0	41.0	37.0	41.0
22-23	38.847765243278566	41.0	41.0	41.0	34.5	41.0
24-25	38.86541065428766	41.0	41.0	41.0	37.0	41.0
26-27	38.836152495494844	41.0	41.0	41.0	37.0	41.0
28-29	38.785000998395965	41.0	41.0	41.0	34.5	41.0
30-31	38.65895660195521	41.0	41.0	41.0	32.0	41.0
32-33	38.702419212438855	41.0	41.0	41.0	32.0	41.0
34-35	38.679023535740924	41.0	41.0	41.0	32.0	41.0
36-37	38.597425912418394	41.0	41.0	41.0	32.0	41.0
38-39	38.50832134158014	41.0	41.0	41.0	32.0	41.0
40-41	38.55379415220505	41.0	41.0	41.0	32.0	41.0
42-43	38.29238706573901	41.0	41.0	41.0	32.0	41.0
44-45	38.40429446410065	41.0	41.0	41.0	32.0	41.0
46-47	38.41815298396625	41.0	41.0	41.0	32.0	41.0
48-49	38.364720234598295	41.0	41.0	41.0	32.0	41.0
50-51	38.250346138105385	41.0	41.0	41.0	32.0	41.0
52-53	38.22458401543305	41.0	41.0	41.0	32.0	41.0
54-55	38.09095490385695	41.0	37.0	41.0	32.0	41.0
56-57	37.88238111661925	41.0	37.0	41.0	29.5	41.0
58-59	37.87334501429109	41.0	37.0	41.0	29.5	41.0
60-61	37.86698931633805	41.0	37.0	41.0	29.5	41.0
62-63	37.85670542533319	41.0	37.0	41.0	29.5	41.0
64-65	37.85928908431413	41.0	37.0	41.0	29.5	41.0
66-67	37.7275620820764	41.0	37.0	41.0	27.0	41.0
68-69	37.727234509185244	41.0	37.0	41.0	27.0	41.0
70-71	37.72813946023909	41.0	37.0	41.0	27.0	41.0
72-73	37.5666171456767	41.0	37.0	41.0	27.0	41.0
74-75	37.50429764081509	41.0	37.0	41.0	27.0	41.0
76-77	36.42746859590872	39.0	34.5	41.0	24.5	41.0
78-79	36.95691075660262	41.0	37.0	41.0	27.0	41.0
80-81	37.599896529872666	41.0	37.0	41.0	27.0	41.0
82-83	37.779838424877724	41.0	37.0	41.0	27.0	41.0
84-85	37.698836126091635	41.0	37.0	41.0	27.0	41.0
86-87	37.561794315578936	41.0	37.0	41.0	27.0	41.0
88-89	37.45218838494188	41.0	37.0	41.0	27.0	41.0
90-91	37.288286216194805	41.0	37.0	41.0	27.0	41.0
92-93	37.18367969147089	41.0	37.0	41.0	27.0	41.0
94-95	37.037319174813355	41.0	37.0	41.0	27.0	41.0
96-97	36.96526283392632	41.0	37.0	41.0	27.0	41.0
98-99	36.84918115028417	41.0	37.0	41.0	27.0	41.0
100	36.725566115266055	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.9151332876348661
1101	2	-0.6519214357079672
1101	3	0.07578422103799376
1101	4	0.1979113312243328
1101	5	-1.0187624956508579
1101	6	-0.6327347300873569
1101	7	-0.39928801813789505
1101	8	-0.13421741988683777
1101	9	-0.007927906054149503
1101	10-11	-0.08372124432424499
1101	12-13	0.045674281842913445
1101	14-15	0.07527665223102531
1101	16-17	-0.014340557923027575
1101	18-19	0.0047006211607723
1101	20-21	0.1612384006324561
1101	22-23	0.13586562886811038
1101	24-25	0.10181340112416848
1101	26-27	0.21743423037183618
1101	28-29	0.1615989321157585
1101	30-31	0.21551191406462777
1101	32-33	-0.0779673587941545
1101	34-35	0.12348937374667912
1101	36-37	-0.059256735098649926
1101	38-39	0.02683706759317772
1101	40-41	0.22086202114982711
1101	42-43	0.27007888008385095
1101	44-45	0.09593804275844064
1101	46-47	0.19247314537969373
1101	48-49	0.24606278950674465
1101	50-51	0.11822773987978508
1101	52-53	0.16331509459431715
1101	54-55	0.0920783511452754
1101	56-57	0.42963354689459976
1101	58-59	0.015115241358586218
1101	60-61	0.012086002842941923
1101	62-63	-0.1739570772749417
1101	64-65	0.19387778792491162
1101	66-67	-0.029426203982183097
1101	68-69	0.26301548834602073
1101	70-71	0.045650214434843406
1101	72-73	0.2779791378441203
1101	74-75	0.32685303056874915
1101	76-77	-0.10430148277871609
1101	78-79	-0.02728581131820107
1101	80-81	0.1943901540703692
1101	82-83	0.09807553285295256
1101	84-85	0.1528442664314369
1101	86-87	0.10694392680712639
1101	88-89	-0.2890240478430357
1101	90-91	-0.24812038635896982
1101	92-93	-0.1550282489300372
1101	94-95	-5.147231457911516E-4
1101	96-97	-0.2204622709882429
1101	98-99	-0.3799529377033366
1101	100	-0.5502182915486102
1102	1	1.4718036701469401
1102	2	0.7444026273720823
1102	3	0.03964547942257113
1102	4	0.10376338073637825
1102	5	-0.7300771679161535
1102	6	-0.0977559526466969
1102	7	0.06731986381042532
1102	8	-0.27895586859067834
1102	9	-0.15648626143725153
1102	10-11	-0.22477838804253736
1102	12-13	-0.28203094271492546
1102	14-15	-0.0905238638512742
1102	16-17	-0.11471733452724209
1102	18-19	-0.07000294171676558
1102	20-21	-0.051362847354788244
1102	22-23	-0.15784159023410638
1102	24-25	-0.2204069364083523
1102	26-27	-0.0836409887673355
1102	28-29	-0.23496603390812254
1102	30-31	-0.09157751856823637
1102	32-33	-0.14861033205129104
1102	34-35	0.01613565188419841
1102	36-37	-0.10019098531519433
1102	38-39	-0.2587684425061525
1102	40-41	-0.15390155846111497
1102	42-43	0.08114992137197419
1102	44-45	0.12512007936292235
1102	46-47	-0.24933889356341865
1102	48-49	-0.2771020635677601
1102	50-51	-0.11346050135052366
1102	52-53	-0.14011514332318598
1102	54-55	-0.1808278337683049
1102	56-57	-0.14294699003956168
1102	58-59	-0.12175833123647806
1102	60-61	9.566168418402299E-4
1102	62-63	-0.322354940696826
1102	64-65	-0.3159267418864431
1102	66-67	-0.08293239782787509
1102	68-69	-0.11123922504657457
1102	70-71	-0.2887182315475698
1102	72-73	-0.38864231827006535
1102	74-75	-0.518840383804779
1102	76-77	-0.12064570250507956
1102	78-79	-0.09624059394564455
1102	80-81	-0.3288426798348638
1102	82-83	-0.30232903009080303
1102	84-85	-0.2662828553019878
1102	86-87	-0.1357973594913986
1102	88-89	-0.19661851864746893
1102	90-91	-0.3704250509219733
1102	92-93	-0.3714302633626474
1102	94-95	-0.1089533882662792
1102	96-97	-0.2289562447846052
1102	98-99	-0.3464926188182389
1102	100	-0.4153247431494478
1103	1	1.4434944571855315
1103	2	0.7520873068543885
1103	3	0.3921691619584262
1103	4	1.0279399920473224
1103	5	2.9144394462216034
1103	6	-0.10878188647054543
1103	7	-0.3392080151015904
1103	8	-0.11367235289605304
1103	9	-0.15696736735522165
1103	10-11	-0.0948055806878969
1103	12-13	-0.21849584858416904
1103	14-15	-0.3965203570526583
1103	16-17	-0.21507438498947806
1103	18-19	-0.23734742656923657
1103	20-21	-0.08411481202284676
1103	22-23	-0.052176619425843285
1103	24-25	-0.154538581327067
1103	26-27	-0.17800944648607953
1103	28-29	-0.15450146871758363
1103	30-31	-0.04296207381241146
1103	32-33	-0.28404278964398344
1103	34-35	-0.22807092636583803
1103	36-37	-0.13678163633346685
1103	38-39	-0.07756641752289539
1103	40-41	-0.14414654800670945
1103	42-43	0.03673998624934427
1103	44-45	-0.3156464659883298
1103	46-47	-0.2976321549066867
1103	48-49	-0.27405319466900835
1103	50-51	-0.09796795461052454
1103	52-53	-0.10549972094438687
1103	54-55	-0.17318505411321894
1103	56-57	-0.09593937979791889
1103	58-59	-0.3364217637388265
1103	60-61	-0.04262171517056146
1103	62-63	0.17086584992893705
1103	64-65	-0.24150979453659716
1103	66-67	-0.36133482934221206
1103	68-69	-0.17861163550595194
1103	70-71	-0.321116730643233
1103	72-73	-0.39635302816274276
1103	74-75	-0.37832480357666043
1103	76-77	-0.33949352347431727
1103	78-79	-0.21218198259497
1103	80-81	-0.009566453111816031
1103	82-83	-0.28830229229261306
1103	84-85	-0.1827897431997485
1103	86-87	-0.09476738182046773
1103	88-89	0.021108624654281982
1103	90-91	-0.383723964746018
1103	92-93	-0.047859361434049674
1103	94-95	-0.059165775864570946
1103	96-97	0.010708141024444728
1103	98-99	-0.11335421672466595
1103	100	0.10445140445484924
1104	1	1.5470538165225598
1104	2	-0.6814333903526126
1104	3	-0.016154985626016583
1104	4	-0.06497937174228241
1104	5	-1.1591740253171245
1104	6	-0.5454976769749607
1104	7	-0.594906239405681
1104	8	-0.10327092123559822
1104	9	0.08244569619999709
1104	10-11	-0.14881732228048605
1104	12-13	-0.023369295407398738
1104	14-15	-0.03819751429489315
1104	16-17	-0.1365016482921888
1104	18-19	-0.3056851258757334
1104	20-21	-0.045366309398069404
1104	22-23	-0.0033316450755052074
1104	24-25	-0.16458366828510407
1104	26-27	-0.2956022745119995
1104	28-29	-0.04081596150836475
1104	30-31	-0.03556243634638889
1104	32-33	-0.24024784096293672
1104	34-35	-0.19570256432515976
1104	36-37	-0.18171432459744352
1104	38-39	-0.15159466228620744
1104	40-41	0.05290422037454334
1104	42-43	-0.09810773123928129
1104	44-45	-0.2396593646042149
1104	46-47	-0.2546007030410493
1104	48-49	-0.32351571000072
1104	50-51	-0.1542035596586544
1104	52-53	-0.26205190234900755
1104	54-55	-0.21966817669972016
1104	56-57	-0.35989946641993953
1104	58-59	-0.2254184259228893
1104	60-61	-0.23826373262198075
1104	62-63	-0.20352610661132786
1104	64-65	-0.406124303701219
1104	66-67	-0.7603087967797393
1104	68-69	-0.645988428112652
1104	70-71	-0.3290458044218312
1104	72-73	-0.33460909150758056
1104	74-75	-0.46657808406104806
1104	76-77	-0.48756259938220126
1104	78-79	-0.3868993144114725
1104	80-81	-0.4718082331078719
1104	82-83	-0.23356769981307934
1104	84-85	-0.33552640354088936
1104	86-87	-0.18537962728400004
1104	88-89	-0.3571370739787909
1104	90-91	-0.5104993084639275
1104	92-93	-0.49324797692517564
1104	94-95	-0.29428873095289987
1104	96-97	-0.3049733122153029
1104	98-99	-0.4054506390284658
1104	100	-0.346926250030279
1105	1	1.4234781751165695
1105	2	0.3420763314171875
1105	3	-0.11870043504574568
1105	4	-0.08390621416087463
1105	5	-1.1678473139008716
1105	6	0.4301741670150854
1105	7	0.07081623253882441
1105	8	-0.08239291925470837
1105	9	0.0805222876372298
1105	10-11	-0.07864352393730911
1105	12-13	-0.16791357003481977
1105	14-15	-0.074956356126755
1105	16-17	-0.13722009083561204
1105	18-19	0.07073482779771467
1105	20-21	-0.018314040390457365
1105	22-23	0.10134666687964256
1105	24-25	0.062349689891981086
1105	26-27	-0.12663645955874614
1105	28-29	-0.08963492521996841
1105	30-31	-0.23395807617981035
1105	32-33	-0.17997443194866491
1105	34-35	-0.1659728744778235
1105	36-37	-0.1444592684644448
1105	38-39	-0.08994735854910374
1105	40-41	-0.14033821301809724
1105	42-43	0.05265144580702952
1105	44-45	-0.02780184704565869
1105	46-47	-0.2622673571536893
1105	48-49	-0.2751055229171371
1105	50-51	-0.24820951995666718
1105	52-53	-0.15930118363116463
1105	54-55	-0.10614434376690696
1105	56-57	-0.11639083266551609
1105	58-59	-0.07474756753381939
1105	60-61	-0.17916974108779726
1105	62-63	-0.31086631206122917
1105	64-65	-0.37556208386793344
1105	66-67	-0.3489366794423461
1105	68-69	-0.4449106321498917
1105	70-71	-0.41829434039141944
1105	72-73	-0.19164177844084662
1105	74-75	-0.3466357312564199
1105	76-77	-0.5522490780600791
1105	78-79	-0.30567070718348077
1105	80-81	-0.4650745411699546
1105	82-83	-0.2455774954754233
1105	84-85	-0.1571309751436445
1105	86-87	-0.1835685441955235
1105	88-89	-0.21510206234869855
1105	90-91	-0.15283903047379255
1105	92-93	-0.16636146507825345
1105	94-95	-0.16066854919265694
1105	96-97	-0.29589223811066745
1105	98-99	-0.29508689610580774
1105	100	-0.2835304130766545
1106	1	1.37083778232134
1106	2	-0.7697877258001693
1106	3	-0.12852508188922762
1106	4	-0.14679153637679576
1106	5	-1.2383150537468204
1106	6	-0.7427394007897092
1106	7	-0.4056245459631569
1106	8	-0.23544011958781397
1106	9	-0.2130268947398406
1106	10-11	-0.22324282782803806
1106	12-13	-0.2775703854800682
1106	14-15	-0.21288248258154852
1106	16-17	-0.23997192350595498
1106	18-19	-0.07825567078307216
1106	20-21	-0.1636117932651615
1106	22-23	-0.2074358091566637
1106	24-25	-0.3081322182429105
1106	26-27	-0.38596207500192037
1106	28-29	-0.25231623707353634
1106	30-31	-0.20562095669261282
1106	32-33	-0.27143052043503957
1106	34-35	-0.19845443512134864
1106	36-37	-0.4792956897193008
1106	38-39	-0.14016560219253904
1106	40-41	-0.22150831870833088
1106	42-43	-0.198847598158423
1106	44-45	-0.30350396808616864
1106	46-47	-0.4797095390528625
1106	48-49	-0.33678231608308096
1106	50-51	-0.27460196596226893
1106	52-53	-0.34015094300855253
1106	54-55	-0.3572681663799244
1106	56-57	-0.28192497499226477
1106	58-59	-0.27482254681215323
1106	60-61	-0.4745289548734135
1106	62-63	-0.5444836322095341
1106	64-65	-0.5456285621728938
1106	66-67	-0.664935667297911
1106	68-69	-0.44091155677156024
1106	70-71	-0.7257622418511929
1106	72-73	-0.5355660521860486
1106	74-75	-0.6938770960742673
1106	76-77	-0.6273714474486027
1106	78-79	-0.4447008336452072
1106	80-81	-0.45823303406262994
1106	82-83	-0.5807757063714902
1106	84-85	-0.641052675199667
1106	86-87	-0.5262033736626179
1106	88-89	-0.5100973200880716
1106	90-91	-0.7981839026580602
1106	92-93	-0.5292196855671634
1106	94-95	-0.5664806239544262
1106	96-97	-0.5741775805174782
1106	98-99	-0.7761021471639893
1106	100	-0.6494661202410796
1107	1	1.5765481511972599
1107	2	-0.6584451752228411
1107	3	-0.1330278254213333
1107	4	-0.2119875104354083
1107	5	-1.117094391389756
1107	6	-0.13755278126804882
1107	7	-0.2238367473509797
1107	8	-0.21346132087790437
1107	9	-0.24650601457565102
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1201	68-69	0.02911989262319281
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1201	96-97	0.03434028385569832
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1202	4	1.0071139180178932
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1202	36-37	-0.20595814986749872
1202	38-39	-0.2547878317855279
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1202	56-57	0.05980368815544068
1202	58-59	0.06972154691633392
1202	60-61	-0.21579978124267285
1202	62-63	-0.39782017009799375
1202	64-65	-0.23196372581683278
1202	66-67	0.0906764070512196
1202	68-69	-0.213847231589547
1202	70-71	-0.20024780710102874
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1202	76-77	0.1972294731484041
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1202	82-83	-0.05780375357086598
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1203	3	-0.37936140642881355
1203	4	0.05498320329896522
1203	5	2.9732283725094497
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1203	7	-0.17056411221827972
1203	8	-0.450234349163253
1203	9	-0.09535595289757737
1203	10-11	0.0019268446636928616
1203	12-13	-0.08235727249001457
1203	14-15	-0.042544683877252965
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2228	2	1.4054540785085017
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2228	90-91	0.20605378111670092
2228	92-93	0.39970634029045016
2228	94-95	0.7255833778326988
2228	96-97	0.5086872987810622
2228	98-99	0.4760316384216523
2228	100	0.5401067997706477
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	17.0
13	23.0
14	106.0
15	404.0
16	1614.0
17	4116.0
18	6673.0
19	8638.0
20	9969.0
21	11537.0
22	13453.0
23	15154.0
24	17132.0
25	19461.0
26	21897.0
27	24475.0
28	27990.0
29	31733.0
30	36174.0
31	42192.0
32	49423.0
33	58455.0
34	71835.0
35	93047.0
36	130317.0
37	215574.0
38	526536.0
39	984788.0
40	1155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.9516272897612	19.85104134726294	18.964333955118843	35.23299740785702
2	18.655953129996185	52.63567442676046	11.845733323706279	16.862639119537075
3	41.36721663707234	14.97276276791667	23.28106744206003	20.378953152950963
4	17.30022179242605	16.187752899473903	23.331853616998803	43.180171691101236
5	17.278851168040767	35.00508274309704	26.653087931455577	21.06297815740661
6	17.565498075818684	27.601399074544698	30.984641204544104	23.848461645092513
7	20.384440205157993	26.57102968453988	36.18343751856521	16.861092591736913
8	20.54678268962922	27.812464932373114	34.30583426296925	17.334918115028415
9	20.9002643686507	28.17378525740463	27.523631454918707	23.402318919025962
10-11	19.283564257094387	30.19349491395642	28.87827325354967	21.644667575399524
12-13	23.901681925897567	26.650942617810724	27.412405193639312	22.0349702626524
14-15	21.820748318404153	27.357947231885298	28.500615539991948	22.3206889097186
16-17	20.846177711181372	29.74017363838593	28.335591413464652	21.078057236968046
18-19	21.465513258038325	29.689841279795104	29.731014799363663	19.1136306628029
20-21	22.73852999808572	29.1003750998396	29.818848890707823	18.34224601136686
22-23	21.939875109741045	30.206923752252578	28.68602014614537	19.16718099186101
24-25	22.25876360623923	28.34619421359403	26.447034681470434	22.94800749869631
26-27	20.11427508201699	26.43810275062214	30.078906286099027	23.36871588126184
28-29	19.446639615014185	28.1131523962702	30.685472866557213	21.754735122158404
30-31	19.977191228525488	30.242541247030797	28.25768712950703	21.522580394936682
32-33	20.589276402210004	30.145225356947186	28.79409032100493	20.47140791983788
34-35	21.361259266104703	29.08189239766854	30.100462562626657	19.4563857736001
36-37	22.06407639296865	28.148144633745453	28.63552276342801	21.15225620985788
38-39	21.47546674967464	29.060196665365023	28.996042887728617	20.46829369723171
40-41	20.72874311862828	29.829306294195028	29.869696674023437	19.572253913153258
42-43	20.565282360639074	29.980102199525888	29.228086634437428	20.226528805397614
44-45	20.661020641217746	30.04559204055633	28.65990507812242	20.633482240103504
46-47	19.914169322917914	28.063957964256748	28.901038103968823	23.12083460885652
48-49	20.824477038543034	28.13717052933139	30.353300152482294	20.685052279643283
50-51	21.89523608351541	30.72703854303499	28.17052603090572	19.20719934254388
52-53	20.44400859046735	30.28224318382804	28.246679507122863	21.02706871858174
54-55	22.306104137227706	28.406115010172496	28.1380257414389	21.149755111160903
56-57	21.565086251803898	28.804172353003676	29.267063395050073	20.36367800014235
58-59	21.150200439313956	28.045680069105195	28.94246315789039	21.861656333690455
60-61	19.643711734904368	30.211747281218543	29.816863871148737	20.327677112728352
62-63	20.387860793121312	31.254890707073752	27.18153128721863	21.17571721258631
64-65	19.903873327323698	30.213190807319656	28.894832056801373	20.988103808555277
66-67	20.221891440528605	30.17864274256896	29.52708623500756	20.07237958189487
68-69	20.90628774926894	29.837764781211014	29.252300436323793	20.003647033196252
70-71	19.49650726436205	30.844391325011717	29.54530077297301	20.113800637653227
72-73	20.17655106176523	30.633779283531254	28.541232103133478	20.64843755157004
74-75	20.00436901374981	29.024175209415613	30.70145980342326	20.269995973411312
76-77	19.55358498412468	30.1885441901606	28.77076003511713	21.487110790597587
78-79	19.445741717439088	29.4284843194075	28.4855158324147	22.640258130738715
80-81	20.709060480835817	29.407003265627246	28.494232499210458	21.38970375432648
82-83	19.75852432125577	28.434523377317767	30.246034470239547	21.560917831186917
84-85	20.415877301261446	29.18484682460576	28.43227492359383	21.96700095053897
86-87	21.403402302416612	28.451624002429156	29.33869056656083	20.8062831285934
88-89	21.597676955370876	28.613203250315195	28.944427300271297	20.84469249404263
90-91	21.63443607955483	28.902820592370603	29.232806961377754	20.229936366696812
92-93	21.003528215825153	28.519077614147186	30.918817206075527	19.558576963952127
94-95	21.57937990534216	29.04983646109061	29.544723188530163	19.826060445037065
96-97	20.525535833338836	28.910700494412282	30.482225251331744	20.081538420917138
98-99	22.44689111048035	29.131110843405306	28.896054603182986	19.525943442931357
100	22.210267141056022	30.830797462589032	28.220404573148596	18.738530823206354
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.0
2	17.0
3	22.5
4	25.0
5	25.0
6	30.5
7	39.5
8	50.5
9	61.5
10	69.5
11	78.5
12	87.5
13	106.0
14	139.5
15	181.5
16	218.0
17	301.0
18	430.0
19	664.5
20	1149.5
21	1764.5
22	2618.5
23	3996.5
24	6239.5
25	9481.5
26	13730.5
27	18560.0
28	23731.0
29	29617.5
30	36589.0
31	44613.0
32	53376.5
33	62905.5
34	73869.0
35	86279.0
36	97935.0
37	105697.5
38	110394.0
39	114287.0
40	116222.5
41	116853.5
42	115658.5
43	113352.0
44	111786.0
45	108070.5
46	101013.5
47	93218.0
48	85880.5
49	80625.5
50	77829.5
51	72730.5
52	64462.5
53	55405.0
54	45935.5
55	36705.0
56	28692.5
57	21097.5
58	14433.5
59	10105.0
60	7826.0
61	6856.0
62	5874.5
63	4515.5
64	3447.5
65	3208.5
66	3990.0
67	3911.0
68	2599.5
69	2391.0
70	3089.0
71	2907.5
72	1704.5
73	701.0
74	295.0
75	178.0
76	132.5
77	95.0
78	72.0
79	57.0
80	46.5
81	36.0
82	36.5
83	27.5
84	17.0
85	20.5
86	16.5
87	13.0
88	13.5
89	16.5
90	17.0
91	10.5
92	8.0
93	12.5
94	13.0
95	6.5
96	10.0
97	17.5
98	39.5
99	90.0
100	80.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.333073145293842
2	0.006724733155987405
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.6816420560685976E-4
30-31	0.010685312192642566
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0010107727749797022
40-41	0.001794637375984369
42-43	8.251206326364915E-5
44-45	0.0
46-47	0.0018977774550639303
48-49	0.0
50-51	0.0
52-53	0.001588357217825246
54-55	0.01821453796545055
56-57	0.013016277979840653
58-59	0.013882654644108969
60-61	3.300482530545966E-4
62-63	0.011819853062517741
64-65	1.237680948954737E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.856521423432106E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2423888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.25147498811306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.39450557010562	64.14919469449093
2	8.447451149804927	12.54471415526095
3	2.332462333184177	5.195663057794223
4	1.003889630563502	2.981611431784477
5	0.5210650267535465	1.934492340058571
6	0.3161997088147034	1.4086976862182148
7	0.20803666638381157	1.0812920531425605
8	0.14768117237409661	0.877243590140036
9	0.10923169037701615	0.7299552713344492
>10	0.4849613042678342	6.205271869243482
>50	0.023226228393480607	1.1786994270808908
>100	0.010610591088989664	1.3771304521737555
>500	6.223505642721827E-4	0.2910838088496331
>1k	5.657732402347032E-5	0.0449501624279669
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.125603163182457E-5	0.0
2	0.0	0.0	0.0	1.237680948954737E-4	0.0
3	0.0	0.0	0.0	1.237680948954737E-4	0.0
4	0.0	0.0	0.0	1.237680948954737E-4	0.0
5	0.0	0.0	0.0	1.237680948954737E-4	0.0
6	0.0	0.0	0.0	1.237680948954737E-4	0.0
7	0.0	0.0	0.0	1.237680948954737E-4	0.0
8	0.0	0.0	0.0	1.237680948954737E-4	0.0
9	0.0	0.0	0.0	1.237680948954737E-4	0.0
10-11	0.0	0.0	0.0	1.237680948954737E-4	0.0
12-13	0.0	0.0	0.0	1.237680948954737E-4	0.0
14-15	0.0	0.0	0.0	1.237680948954737E-4	0.0
16-17	0.0	0.0	0.0	1.237680948954737E-4	0.0
18-19	0.0	0.0	0.0	1.237680948954737E-4	0.0
20-21	0.0	0.0	0.0	1.237680948954737E-4	0.0
22-23	0.0	0.0	0.0	1.237680948954737E-4	0.0
24-25	0.0	0.0	0.0	1.237680948954737E-4	0.0
26-27	0.0	0.0	0.0	1.237680948954737E-4	0.0
28-29	0.0	0.0	0.0	1.237680948954737E-4	0.0
30-31	0.0	0.0	0.0	1.237680948954737E-4	0.0
32-33	0.0	0.0	0.0	1.237680948954737E-4	0.0
34-35	4.125603163182457E-5	0.0	0.0	1.237680948954737E-4	0.0
36-37	6.188404744773685E-5	0.0	0.0	1.237680948954737E-4	0.0
38-39	8.251206326364915E-5	0.0	0.0	1.237680948954737E-4	0.0
40-41	8.251206326364915E-5	0.0	0.0	1.237680948954737E-4	0.0
42-43	8.251206326364915E-5	0.0	0.0	1.237680948954737E-4	0.0
44-45	8.251206326364915E-5	0.0	0.0	1.237680948954737E-4	0.0
46-47	8.251206326364915E-5	0.0	0.0	1.237680948954737E-4	0.0
48-49	1.237680948954737E-4	0.0	0.0	1.237680948954737E-4	0.0
50-51	1.237680948954737E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
52-53	1.237680948954737E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
54-55	1.237680948954737E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
56-57	1.650241265272983E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
58-59	1.856521423432106E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
60-61	2.0628015815912285E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
62-63	2.0628015815912285E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
64-65	3.094202372386843E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
66-67	3.919323005023334E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
68-69	5.157003953978072E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
70-71	5.569564270296317E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
72-73	5.77584442845544E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
74-75	7.426085693728423E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
76-77	9.695167433478774E-4	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
78-79	0.0010520288066115267	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
80-81	0.0012789369805865617	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
82-83	0.0014852171387456847	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
84-85	0.0016502412652729828	0.0	0.0	1.237680948954737E-4	4.125603163182457E-5
86-87	0.0016502412652729828	8.251206326364915E-5	0.0	1.237680948954737E-4	4.125603163182457E-5
88	0.0016502412652729828	8.251206326364915E-5	0.0	1.237680948954737E-4	4.125603163182457E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9400	0.0	79.19473	8
CACCGGC	9470	0.0	75.88329	9
CGGGGCA	910	0.0	75.82229	94
CTTCACC	10405	0.0	74.748314	6
AGGCTGC	45	2.6593625E-9	73.0231	2
GTGTGGC	4315	0.0	71.24944	7
CCTTCAC	11610	0.0	70.709625	5
CAGCTGC	85	0.0	68.4845	1
CGTAGTG	1790	0.0	67.39087	4
TTCACCG	11930	0.0	64.406425	7
CGGGCCC	680	0.0	64.194084	7
TGTGGCA	4720	0.0	64.04199	8
GCAGCCG	25	0.003273671	63.503815	1
ACCTTCA	4695	0.0	62.08361	4
GCCTTCA	3225	0.0	61.56468	4
GCAGCCA	85	0.0	60.750305	2
CGCTGCA	35	1.6348234E-4	60.47982	1
CAGCGTA	870	0.0	60.21913	1
GCGTAGT	1270	0.0	59.873173	3
TGTGTGG	4890	0.0	58.935974	6
>>END_MODULE