FastQCFastQC Report
Tue 7 Mar 2017
1NF_7A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_7A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2590507
Sequences flagged as poor quality0
Sequence length96
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA157710.6087997446059786No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA63610.24555038839887328No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG62720.24211476749532043No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT59910.23126747003578835No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA56820.21933930307850932No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG56090.21652132188795473No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG47300.1825897401551125No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG46180.1782662621641246No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT44840.17309352956776414No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC43780.16900166646915063No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42400.16367452394454057No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA40680.15703489702980922No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT38510.14865815842227023No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA38410.14827213360164632No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38220.14753868644246088No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG38120.14715266162183696No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC34540.13333297304350075No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA29540.11403173201230492No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC29050.11214021039124775No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA28640.11055750862668967No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA28480.10993986891369141No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC26350.101717540234402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAA500.089.999863
AGCTGCT1200.089.999862
GCAGCTA401.4551915E-1189.999863
GCAGCGG450.089.999863
GCTGCCT355.0749804E-1089.9998553
AGCAGCC700.089.9998552
CAGCTGC4800.089.062361
AGCTGCC2400.086.249872
CAGCAGC4850.085.3606951
AGCTGCA850.084.705762
CAGCGTA59600.083.808591
GCTGCCC1450.083.792983
GCTGCCA700.083.57133
CAGCCCT159450.083.424151
AGCAGCA2050.083.4145052
AGCCCTT135700.081.2784352
CAGCTCT63800.080.8306051
CTGCAGC454.1836756E-1179.999881
AGCAGCG1350.079.999882
GCCCTTC123100.079.398743
Produced by FastQC (version 0.11.5)