FastQCFastQC Report
Tue 7 Mar 2017
1NF_7A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_7A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2590507
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG108300.080.483778
CACCGGC106050.078.296459
CTTCACC119250.075.888086
CCTTCAC131250.071.5961465
GTGTGGC44500.069.721447
CGTAGTG22850.066.760884
TTCACCG134200.066.594647
CAGCTGC800.066.159361
CGGGCCC6750.066.0609367
TGCAGCC651.0913936E-1164.991133
GCGTAGT15200.064.230993
GCAGCCT751.2732926E-1163.5129931
TGTGGCA48850.062.8402758
GCCTTCA38150.062.2559434
CCGCGTA2150.061.5435941
GGCGCGC3600.061.280216
GGCCCGA7250.060.857599
ACGGGCC7100.060.8211146
CGGGGCA12950.060.5301394
GGGCCCG7400.060.2582868
Produced by FastQC (version 0.11.5)