##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_7A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2590507
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.804872559695843	12.0	12.0	12.0	2.0	32.0
2	14.624854902920548	12.0	12.0	12.0	12.0	22.0
3	13.729377685526424	12.0	12.0	12.0	12.0	22.0
4	13.782320989674995	12.0	12.0	12.0	12.0	22.0
5	22.450765043290755	22.0	22.0	22.0	22.0	27.0
6	26.81204219868929	27.0	22.0	32.0	22.0	32.0
7	34.997181632784624	37.0	37.0	37.0	27.0	41.0
8	37.608152767006615	41.0	37.0	41.0	27.0	41.0
9	38.567112924226805	41.0	41.0	41.0	32.0	41.0
10-11	38.92377341578309	41.0	41.0	41.0	37.0	41.0
12-13	39.101775636969904	41.0	41.0	41.0	37.0	41.0
14-15	39.0558936918526	41.0	41.0	41.0	37.0	41.0
16-17	38.95880072896927	41.0	41.0	41.0	37.0	41.0
18-19	38.89649400677165	41.0	41.0	41.0	37.0	41.0
20-21	38.90687903950848	41.0	41.0	41.0	37.0	41.0
22-23	38.89468297132569	41.0	41.0	41.0	37.0	41.0
24-25	38.917543168190626	41.0	41.0	41.0	37.0	41.0
26-27	38.88480150796736	41.0	41.0	41.0	37.0	41.0
28-29	38.83156965026537	41.0	41.0	41.0	37.0	41.0
30-31	38.71291874524948	41.0	41.0	41.0	32.0	41.0
32-33	38.75646002886694	41.0	41.0	41.0	32.0	41.0
34-35	38.73245353129715	41.0	41.0	41.0	32.0	41.0
36-37	38.65662049938487	41.0	41.0	41.0	32.0	41.0
38-39	38.557214668788774	41.0	41.0	41.0	32.0	41.0
40-41	38.6037221671279	41.0	41.0	41.0	32.0	41.0
42-43	38.341390700739275	41.0	41.0	41.0	32.0	41.0
44-45	38.449722390250244	41.0	41.0	41.0	32.0	41.0
46-47	38.46359573627865	41.0	41.0	41.0	32.0	41.0
48-49	38.403346912399776	41.0	41.0	41.0	32.0	41.0
50-51	38.292841131099046	41.0	41.0	41.0	32.0	41.0
52-53	38.27306604460054	41.0	41.0	41.0	32.0	41.0
54-55	38.137447804618944	41.0	39.0	41.0	32.0	41.0
56-57	37.9363198014906	41.0	37.0	41.0	29.5	41.0
58-59	37.93220458389034	41.0	37.0	41.0	29.5	41.0
60-61	37.94436706791373	41.0	37.0	41.0	32.0	41.0
62-63	37.934209210783834	41.0	37.0	41.0	32.0	41.0
64-65	37.929982818035235	41.0	37.0	41.0	32.0	41.0
66-67	37.79717483874778	41.0	37.0	41.0	27.0	41.0
68-69	37.80428039762101	41.0	37.0	41.0	27.0	41.0
70-71	37.80858669750748	41.0	37.0	41.0	29.5	41.0
72-73	37.62320387476274	41.0	37.0	41.0	27.0	41.0
74-75	37.57709900031152	41.0	37.0	41.0	27.0	41.0
76-77	36.50407314089482	39.0	34.5	41.0	24.5	41.0
78-79	37.03156756573134	41.0	37.0	41.0	27.0	41.0
80-81	37.6914409032672	41.0	37.0	41.0	27.0	41.0
82-83	37.871617023231366	41.0	37.0	41.0	32.0	41.0
84-85	37.79989148842292	41.0	37.0	41.0	27.0	41.0
86-87	37.656834357135494	41.0	37.0	41.0	27.0	41.0
88-89	37.56400484538355	41.0	37.0	41.0	27.0	41.0
90-91	37.42651110381095	41.0	37.0	41.0	27.0	41.0
92-93	37.32302228868711	41.0	37.0	41.0	27.0	41.0
94-95	37.16408660544056	41.0	37.0	41.0	27.0	41.0
96-97	37.094876408363305	41.0	37.0	41.0	27.0	41.0
98-99	36.98448489040948	41.0	37.0	41.0	27.0	41.0
100	36.8523632632531	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.2427563809175464
1101	2	-0.46227095996368917
1101	3	0.017995417157827376
1101	4	-0.019658479364631276
1101	5	-1.0867514762699457
1101	6	-0.6154582477681032
1101	7	-0.11851045987378228
1101	8	-0.13495843701349486
1101	9	-0.08986634384847747
1101	10-11	-3.3674002892780663E-4
1101	12-13	0.08270158668006644
1101	14-15	-0.022781792359587882
1101	16-17	-0.12164794589578065
1101	18-19	0.028997382718110032
1101	20-21	0.05068909447626169
1101	22-23	0.03242916681283958
1101	24-25	0.07591388122460074
1101	26-27	0.02560156970255889
1101	28-29	-0.06562893939219094
1101	30-31	0.06000965635123379
1101	32-33	-0.1974807207721483
1101	34-35	0.002762550944240161
1101	36-37	0.11142234485183877
1101	38-39	0.12476509639126476
1101	40-41	-0.05034269153794213
1101	42-43	-0.004468323234235072
1101	44-45	0.018318631027526067
1101	46-47	-0.04297600518042799
1101	48-49	0.16288860964595386
1101	50-51	-0.0347347780513374
1101	52-53	0.012301843295233539
1101	54-55	0.09072701151157503
1101	56-57	0.27833886692281595
1101	58-59	0.07547363303454091
1101	60-61	0.09372593624535597
1101	62-63	0.04981217824160211
1101	64-65	-0.006826475682252919
1101	66-67	-0.04016939448828083
1101	68-69	-0.005403413394326151
1101	70-71	-0.03394745035047464
1101	72-73	0.10597719140618267
1101	74-75	0.055164371767681075
1101	76-77	-0.19710127685696932
1101	78-79	0.013437975434776206
1101	80-81	0.019693308670852616
1101	82-83	-0.11108238230786327
1101	84-85	-0.07836877874539994
1101	86-87	0.02171181606387762
1101	88-89	-0.17953674391242913
1101	90-91	-0.06261157122361283
1101	92-93	0.08891474031231184
1101	94-95	-0.038378101695776934
1101	96-97	-0.2155353672686786
1101	98-99	-0.4668741531008749
1101	100	-0.38755909597527705
1102	1	1.8561795621144288
1102	2	0.969289662186755
1102	3	-0.17545115192577043
1102	4	0.05247505677257713
1102	5	-0.7700941479230394
1102	6	-0.018124823804651413
1102	7	0.07536976672935936
1102	8	-0.3012810952961189
1102	9	-0.3250290954775821
1102	10-11	-0.14380019783247633
1102	12-13	-0.3548633124271561
1102	14-15	-0.13652746361642443
1102	16-17	-0.20560719348100065
1102	18-19	-0.0447054088477401
1102	20-21	0.16381313932374297
1102	22-23	0.039472494021680404
1102	24-25	-0.015440233366931011
1102	26-27	-0.02278807803425309
1102	28-29	-0.1708697778549535
1102	30-31	-0.13813034401710667
1102	32-33	-0.2009278613571155
1102	34-35	0.08933665464117269
1102	36-37	-0.08556848207646794
1102	38-39	0.059924753179060986
1102	40-41	-0.11563938144376351
1102	42-43	0.012105216556022924
1102	44-45	-0.09479107881876558
1102	46-47	-0.4787847242407963
1102	48-49	-0.5084685770027804
1102	50-51	-0.1375511026367775
1102	52-53	-0.09055150546546997
1102	54-55	-0.2889710003281891
1102	56-57	-0.0065164036773879275
1102	58-59	0.03997884100693483
1102	60-61	0.051449359580338694
1102	62-63	-0.24864150233165816
1102	64-65	-0.18781336551672467
1102	66-67	-0.07303100818411679
1102	68-69	-0.34941486696535407
1102	70-71	-0.40537653984626587
1102	72-73	-0.21444445129381506
1102	74-75	-0.4161566071363225
1102	76-77	-0.12266920397212289
1102	78-79	-0.28793140640770076
1102	80-81	-0.17890655094107188
1102	82-83	-0.3135080733640976
1102	84-85	-0.14654746842755628
1102	86-87	-0.17629708075050132
1102	88-89	-0.19653672625474172
1102	90-91	-0.42226308069923846
1102	92-93	-0.2927896970255972
1102	94-95	-0.1691839433131861
1102	96-97	-0.19227801450480086
1102	98-99	-0.2623279261396405
1102	100	-0.36103706535685376
1103	1	1.5758185862246812
1103	2	0.7940437649277214
1103	3	0.2837540004617516
1103	4	0.9922157550327153
1103	5	3.0828029319882475
1103	6	0.20554544081138104
1103	7	-0.2546820995563692
1103	8	-0.12515363941610502
1103	9	0.10455180791218055
1103	10-11	-0.08943015895007989
1103	12-13	-0.2355028679664386
1103	14-15	-0.11253774780725223
1103	16-17	-0.12532479387605377
1103	18-19	-0.1180021392232149
1103	20-21	0.11649655903491407
1103	22-23	0.016856576683139224
1103	24-25	-0.02638539523506722
1103	26-27	-0.04865541078502389
1103	28-29	-0.07307990904963901
1103	30-31	-0.05790375930465075
1103	32-33	-0.08619589654425397
1103	34-35	-0.09464635150538925
1103	36-37	-0.07222149850525028
1103	38-39	-0.009492842168910443
1103	40-41	-0.08748433083925278
1103	42-43	0.19255842556597713
1103	44-45	-0.2655140894978132
1103	46-47	-0.21610549064656936
1103	48-49	-0.11717233550243122
1103	50-51	-0.011440025062988468
1103	52-53	0.07558468880993985
1103	54-55	-0.06885972680849761
1103	56-57	-0.06298583463267704
1103	58-59	-0.08496784290561976
1103	60-61	0.0091130708946352
1103	62-63	-0.042474946338138864
1103	64-65	-0.10012094515932546
1103	66-67	-0.22557716680331197
1103	68-69	-0.21236684141717177
1103	70-71	-0.22005833063344227
1103	72-73	-0.09136053993658066
1103	74-75	-0.23709322853421355
1103	76-77	-0.3831174252682814
1103	78-79	-0.36227654630614126
1103	80-81	-0.024640620687911508
1103	82-83	-0.10224494474144308
1103	84-85	-0.23950172891485977
1103	86-87	-0.2762868876052522
1103	88-89	-0.14017902404699356
1103	90-91	-0.6503303514994414
1103	92-93	-0.21783752069012507
1103	94-95	-0.24370870028584335
1103	96-97	4.546687763564705E-4
1103	98-99	-0.12515253575702445
1103	100	-0.08267849821618967
1104	1	1.6665490078291683
1104	2	-0.46770694688302505
1104	3	-0.06441704068373966
1104	4	0.10734983961976674
1104	5	-1.112164191144334
1104	6	-0.6110174294320032
1104	7	-0.32609218497366044
1104	8	-0.22756341796277013
1104	9	-0.15256863334592907
1104	10-11	-0.08051154521081827
1104	12-13	-0.07283670844734758
1104	14-15	-0.09691823163000635
1104	16-17	-0.0841644058088562
1104	18-19	0.0330798031784596
1104	20-21	0.1431926151018672
1104	22-23	-0.03509691801713899
1104	24-25	-0.10461585408113194
1104	26-27	0.044934667660641026
1104	28-29	0.002155035668955918
1104	30-31	0.048218061381604116
1104	32-33	0.09535025471218006
1104	34-35	0.08803561830607975
1104	36-37	-0.1358923862154242
1104	38-39	-0.08836465068958432
1104	40-41	-0.050341167251382046
1104	42-43	-6.823765084362776E-4
1104	44-45	0.03761162119568695
1104	46-47	-0.24366843140067118
1104	48-49	-0.38539821611684744
1104	50-51	-0.1393929596498893
1104	52-53	0.03376646546175266
1104	54-55	-0.054789956941597495
1104	56-57	0.09511381191988733
1104	58-59	-0.08442659859985469
1104	60-61	-0.1018869003052103
1104	62-63	0.031180061562047
1104	64-65	-0.1191688715977719
1104	66-67	-0.5315034443633238
1104	68-69	-0.38779365475939187
1104	70-71	-0.056326168109201546
1104	72-73	-0.012521702628660591
1104	74-75	-0.22000679132202805
1104	76-77	-0.3333582045011809
1104	78-79	-0.1405317335617795
1104	80-81	-0.14227366499347482
1104	82-83	-0.09876995767668717
1104	84-85	-0.006571928797882265
1104	86-87	-0.04323851029539583
1104	88-89	-0.11225578341374387
1104	90-91	-0.17758145608472375
1104	92-93	-0.19812004028664632
1104	94-95	-0.21186267982184148
1104	96-97	-0.29293778101454393
1104	98-99	-0.2230409877883801
1104	100	-0.4160003776944947
1105	1	1.4917819331046402
1105	2	0.29996377368724225
1105	3	-0.11409639748054445
1105	4	-0.21887892111430673
1105	5	-1.1213985096177161
1105	6	0.36851749930855604
1105	7	-0.0877612177776399
1105	8	-0.1522819536873783
1105	9	-0.0712435634240478
1105	10-11	-0.22922370408262793
1105	12-13	-0.27762756013674306
1105	14-15	-0.3068874658112932
1105	16-17	-0.3784690805861217
1105	18-19	-0.14878520714763255
1105	20-21	-0.17798132561900815
1105	22-23	-0.13950673617546983
1105	24-25	-0.1276374396935438
1105	26-27	-0.17296924017185233
1105	28-29	-0.26593210093398056
1105	30-31	-0.1608651812407942
1105	32-33	-0.3906379316859585
1105	34-35	-0.2744137158379161
1105	36-37	-0.31105491803252505
1105	38-39	-0.15132845059876843
1105	40-41	-0.25930761141667347
1105	42-43	-0.1257329399514333
1105	44-45	-0.23027383324272677
1105	46-47	-0.2619019471466473
1105	48-49	-0.48024694687202185
1105	50-51	-0.4433532275965959
1105	52-53	-0.31619534163330343
1105	54-55	-0.3726770595148423
1105	56-57	-0.20412107244487743
1105	58-59	-0.12071519329720815
1105	60-61	-0.1884308415805478
1105	62-63	-0.44737275029888934
1105	64-65	-0.5053106679732835
1105	66-67	-0.5596583507464103
1105	68-69	-0.42701450044500433
1105	70-71	-0.44819604281475023
1105	72-73	-0.5306620463590832
1105	74-75	-0.5113146234683512
1105	76-77	-0.4019518615256601
1105	78-79	-0.4073069357822021
1105	80-81	-0.4939489607098295
1105	82-83	-0.5571629366603972
1105	84-85	-0.4586199697371711
1105	86-87	-0.44494041433888754
1105	88-89	-0.40149430129658015
1105	90-91	-0.4396146028390291
1105	92-93	-0.28440704400990313
1105	94-95	-0.16332396570616226
1105	96-97	-0.3058341979313042
1105	98-99	-0.46514180143348227
1105	100	-0.33039149095146314
1106	1	1.3357865263586692
1106	2	-0.5590748614816423
1106	3	-0.015064944682327663
1106	4	-0.21993952537829387
1106	5	-1.212809045530605
1106	6	-0.6134693559743916
1106	7	-0.16808632168768156
1106	8	-0.17716145229165647
1106	9	-0.23289809854717447
1106	10-11	-0.11332986689922109
1106	12-13	-0.08503114255115207
1106	14-15	-0.00846551696837139
1106	16-17	-0.07266440380126937
1106	18-19	-0.09490726340614941
1106	20-21	-0.1397826325620244
1106	22-23	-0.03582490540875227
1106	24-25	-0.17787375587520415
1106	26-27	-0.19220767925598636
1106	28-29	-0.2576639989977707
1106	30-31	-0.12093121914888627
1106	32-33	-0.18880286985941552
1106	34-35	-0.2532782349832843
1106	36-37	-0.37415362537520025
1106	38-39	-0.13796549563295457
1106	40-41	-0.29395624461115943
1106	42-43	-0.052577313826944305
1106	44-45	-0.04628101486051861
1106	46-47	-0.23423815060499464
1106	48-49	-0.21755042217198195
1106	50-51	-0.3141193979531707
1106	52-53	-0.05879873266840718
1106	54-55	-0.08035899643608246
1106	56-57	-0.11180267057505233
1106	58-59	-0.2598108545317288
1106	60-61	-0.09627628283615053
1106	62-63	-0.2855107056458479
1106	64-65	-0.4961890007598484
1106	66-67	-0.48925455508183546
1106	68-69	-0.28722996403634937
1106	70-71	-0.6091917234168349
1106	72-73	-0.3579368542880772
1106	74-75	-0.49411024832048867
1106	76-77	-0.5023996492105667
1106	78-79	-0.23212899047852176
1106	80-81	-0.26720136814975604
1106	82-83	-0.4391053538480989
1106	84-85	-0.5469868443274422
1106	86-87	-0.6114797520286999
1106	88-89	-0.4704074162564851
1106	90-91	-0.5147895178448181
1106	92-93	-0.4255408158518108
1106	94-95	-0.32649732001443255
1106	96-97	-0.27702415827204163
1106	98-99	-0.6395543612465815
1106	100	-0.6614155867133888
1107	1	1.2895535193848353
1107	2	-0.4136761688779451
1107	3	-0.33653620614125046
1107	4	-0.17270476873191143
1107	5	-1.1888451973958531
1107	6	-0.3567073573858828
1107	7	-0.36033639455932587
1107	8	-0.22876850732085785
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2224	36-37	0.21494482395765147
2224	38-39	0.2183963082081135
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2224	44-45	0.35799390723625635
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2224	54-55	0.3397123878834094
2224	56-57	0.510192206767826
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2224	60-61	0.4126354761585489
2224	62-63	0.4180672946685391
2224	64-65	0.46133575720846665
2224	66-67	0.4136414105945079
2224	68-69	0.423362470335384
2224	70-71	0.43217595206191106
2224	72-73	0.5092013927275616
2224	74-75	0.3891067618600985
2224	76-77	0.7382792415584802
2224	78-79	0.4507535675212395
2224	80-81	0.3854343036673029
2224	82-83	0.3770567218451717
2224	84-85	0.1772640364182081
2224	86-87	0.32753233337356846
2224	88-89	0.527725495428534
2224	90-91	0.613102240595083
2224	92-93	0.41334301818888974
2224	94-95	0.5451035565702185
2224	96-97	0.40725261315943584
2224	98-99	0.46170688502007096
2224	100	0.3180736270419615
2225	1	1.4228084663646605
2225	2	1.7219867297559688
2225	3	0.8272601257926961
2225	4	1.0417293700453065
2225	5	3.244743874196768
2225	6	-0.174515817327638
2225	7	0.1971759770617041
2225	8	0.3735490441772029
2225	9	0.20427410821228875
2225	10-11	0.19424612143872366
2225	12-13	0.18778739535083133
2225	14-15	0.14258144090393898
2225	16-17	0.10685485416492924
2225	18-19	0.14008684252201675
2225	20-21	0.1595740126204248
2225	22-23	0.22987639429963735
2225	24-25	0.2579330234510806
2225	26-27	0.16440207739945834
2225	28-29	0.11068871252631851
2225	30-31	0.18686346073300797
2225	32-33	0.10177598768926543
2225	34-35	0.17756976937269542
2225	36-37	0.16291403343698363
2225	38-39	0.25886900005708924
2225	40-41	0.12036409052247166
2225	42-43	0.41987731465126643
2225	44-45	0.3371047877067568
2225	46-47	0.3826036645571378
2225	48-49	0.2858521281507862
2225	50-51	0.2701535558722341
2225	52-53	0.22159088416950112
2225	54-55	0.18041086474499934
2225	56-57	0.36873461884368197
2225	58-59	0.38739644887321845
2225	60-61	0.31834419528381375
2225	62-63	0.39652713133857276
2225	64-65	0.2902771168348366
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2225	68-69	0.38064285344156445
2225	70-71	0.08810208030416078
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2225	74-75	0.40286790316692844
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2225	78-79	0.4402345351192736
2225	80-81	0.3170858117837341
2225	82-83	0.32183717323311356
2225	84-85	0.24641324571545908
2225	86-87	0.3377829729460444
2225	88-89	0.47184406897709863
2225	90-91	0.5331800817990242
2225	92-93	0.4432869361051104
2225	94-95	0.4902347030664558
2225	96-97	0.5334804557290127
2225	98-99	0.33818791634774925
2225	100	0.5767127260869245
2226	1	1.4981871644160325
2226	2	1.2642990025157967
2226	3	0.8572074901314508
2226	4	1.0038920377303207
2226	5	3.077647081806468
2226	6	-0.43957309381905674
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2226	12-13	0.09271031229351223
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2226	18-19	0.15097191805008947
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2226	66-67	0.2006407278243003
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2226	70-71	0.2730283076191071
2226	72-73	0.2476782615803188
2226	74-75	0.18739319318633818
2226	76-77	0.38114706093808337
2226	78-79	0.2583074048743086
2226	80-81	0.11886753058690402
2226	82-83	0.11282576098451358
2226	84-85	-0.018680493116214336
2226	86-87	0.018924446455081068
2226	88-89	0.04453197620947691
2226	90-91	0.11286087210216067
2226	92-93	0.03675262495016085
2226	94-95	0.2651297601833633
2226	96-97	0.24354111124952738
2226	98-99	0.17517314325483824
2226	100	0.25877485049235105
2227	1	1.2884939889924567
2227	2	1.8820218779492173
2227	3	0.7095301713039266
2227	4	0.05271913620353352
2227	5	3.305728188536701
2227	6	0.14466019760393323
2227	7	0.33865899991627657
2227	8	0.34870524564341565
2227	9	0.16768278659074554
2227	10-11	0.14510902057824637
2227	12-13	0.21823575472003398
2227	14-15	0.07381962672206299
2227	16-17	0.3326085107773409
2227	18-19	0.24033139551414706
2227	20-21	0.19004463739880606
2227	22-23	0.23919405571697894
2227	24-25	0.30691490783770803
2227	26-27	0.19314204765726117
2227	28-29	0.17637799186925207
2227	30-31	0.3550829335284291
2227	32-33	0.2502622605605609
2227	34-35	0.2430139459602998
2227	36-37	0.43192919682201847
2227	38-39	0.21181650958402543
2227	40-41	0.3627421278495646
2227	42-43	0.4359582544531122
2227	44-45	0.35213760398781346
2227	46-47	0.38183390446463505
2227	48-49	0.3382711319982121
2227	50-51	0.31625367324667053
2227	52-53	0.3477227778563048
2227	54-55	0.3382854436034819
2227	56-57	0.4473756775409825
2227	58-59	0.4602557397336824
2227	60-61	0.28794824667200913
2227	62-63	0.2569870632229154
2227	64-65	0.3300901423740825
2227	66-67	0.2788867520679261
2227	68-69	0.4846691357871151
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2227	72-73	0.28451689685074655
2227	74-75	0.37951680331985216
2227	76-77	0.569214591688322
2227	78-79	0.5010337625187375
2227	80-81	0.4462329733105648
2227	82-83	0.4592764129472755
2227	84-85	0.34730276255607606
2227	86-87	0.3373605715621011
2227	88-89	0.3337731513581943
2227	90-91	0.3329450580355058
2227	92-93	0.38162448887092637
2227	94-95	0.22870007577382268
2227	96-97	0.4018965604918918
2227	98-99	0.4705865606317232
2227	100	0.39336908469378784
2228	1	1.455081176746198
2228	2	1.5533501823233813
2228	3	-0.35156487013251336
2228	4	-0.12717171684762718
2228	5	3.2235448444936594
2228	6	-0.07820542534196662
2228	7	0.4065464504107297
2228	8	0.2518566612579889
2228	9	0.3403772690357272
2228	10-11	0.21679847836021793
2228	12-13	0.21628397970120972
2228	14-15	0.3467630408047455
2228	16-17	0.3425562019916413
2228	18-19	0.20388334802564856
2228	20-21	0.33404477733078863
2228	22-23	0.38095638860412606
2228	24-25	0.34565866843618664
2228	26-27	0.23537932075110746
2228	28-29	0.34902257751407006
2228	30-31	0.2560202016509976
2228	32-33	0.2719973803589113
2228	34-35	0.19152799715699587
2228	36-37	0.3170744959358487
2228	38-39	0.30206693260210216
2228	40-41	0.3841172910268327
2228	42-43	0.5679940254875575
2228	44-45	0.3821645224829595
2228	46-47	0.42188326731379533
2228	48-49	0.41147167110460003
2228	50-51	0.3100464008340751
2228	52-53	0.3920976961525966
2228	54-55	0.2638176914819468
2228	56-57	0.5166055544928909
2228	58-59	0.6141571723794428
2228	60-61	0.45802176620080814
2228	62-63	0.48131074063550017
2228	64-65	0.36660935264214345
2228	66-67	0.5055831972431193
2228	68-69	0.42415425972819065
2228	70-71	0.5288121277630538
2228	72-73	0.6273349797457257
2228	74-75	0.6465753409201298
2228	76-77	0.6294978434867318
2228	78-79	0.650601722512917
2228	80-81	0.45157524510636193
2228	82-83	0.4414904131424393
2228	84-85	0.4680055157178913
2228	86-87	0.5110741639291803
2228	88-89	0.42565930564057197
2228	90-91	0.639685544961992
2228	92-93	0.6925775729903023
2228	94-95	0.738618859985813
2228	96-97	0.6458552731912661
2228	98-99	0.6312143949448838
2228	100	0.7396143965119677
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	37.0
14	140.0
15	456.0
16	1686.0
17	4159.0
18	6687.0
19	8744.0
20	10288.0
21	11851.0
22	13724.0
23	15403.0
24	18198.0
25	20067.0
26	22759.0
27	25497.0
28	28954.0
29	33198.0
30	37759.0
31	44086.0
32	51816.0
33	61367.0
34	75689.0
35	97929.0
36	137278.0
37	226129.0
38	558862.0
39	1076396.0
40	1329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.946342763640136	18.55945701223456	18.723914292321943	35.770285931803365
2	18.608848326264127	52.84412141558087	11.275958434848203	17.271071823306798
3	42.29284074507423	14.736690539728322	23.38665751530492	19.58381119989253
4	17.265732152045913	15.943751551337249	22.41727198575414	44.373244310862695
5	17.3136764347674	35.06630169306626	26.594523774689666	21.02549809747667
6	17.33394273785016	27.567036105287496	31.102483027453697	23.996538129408645
7	20.253178238854403	26.766111807456994	36.388243691292864	16.59246626239574
8	20.607240204330658	27.71117777330847	34.42438101885075	17.257201003510122
9	20.72119473137884	28.164679732577447	27.836095405262366	23.27803013078135
10-11	19.43580928366532	30.059173744753444	28.951996655480954	21.553020316100284
12-13	24.04731969456172	26.584950359138194	27.44507156321137	21.922658383088717
14-15	21.782106745899547	27.343354022976968	28.522679151224068	22.351860079899417
16-17	20.95840698365223	29.660274996361718	28.276549725594258	21.104768294391793
18-19	21.492009093200675	29.571296275207903	29.762841791201488	19.173852840389934
20-21	22.73554945035856	29.03833110661349	29.965871545608643	18.26024789741931
22-23	22.16967566580596	30.197872462803616	28.660740928320212	18.971710943070217
24-25	22.260951234642484	28.32055655514538	26.512396994101927	22.906095216110206
26-27	20.12025059187256	26.347159069633857	30.142419997320985	23.3901703411726
28-29	19.571314087098308	28.09405759047102	30.738645619490924	21.595982702939747
30-31	20.025005366289818	30.135172593778808	28.223769652588715	21.61605238734266
32-33	20.484349202685035	30.241184447677615	28.74545407520613	20.52901227443122
34-35	21.47575744825241	29.191544357919124	29.914626750670813	19.418071443157654
36-37	21.93126673658863	27.971184791239708	28.854042857247634	21.243505614924025
38-39	21.556863458870048	29.135910747404786	28.92737881107844	20.379846982646725
40-41	20.71394642178489	29.778945903540066	29.881649095561453	19.625458579113594
42-43	20.556092452261712	30.12778012931458	29.243782436973188	20.072344981450524
44-45	20.682881767931917	29.94649309961332	28.75730503719928	20.613320095255485
46-47	19.994634136926017	28.007971587948038	28.954100638885137	23.043293636240808
48-49	20.955033311053263	28.000470178322267	30.40625452976088	20.63824198086359
50-51	21.899535496333343	30.662453334424494	28.179811905545904	19.25819926369626
52-53	20.43074489300381	30.23975222990767	28.226871149824007	21.102631727264516
54-55	22.32351417237894	28.250685897272547	28.336360315949033	21.08943961439948
56-57	21.789692261155945	28.653005299398753	29.38897177611223	20.168330663333077
58-59	21.188938905461345	27.971367587446476	29.056829520703793	21.78286398638839
60-61	19.705242629473382	30.20536355191182	29.770139279873586	20.319254538741212
62-63	20.357063850832112	31.2237159240818	27.29353113976517	21.125689085320914
64-65	19.841594439688826	30.157836172711665	28.95530886382082	21.04526052377869
66-67	20.23067299181203	30.270367924116787	29.434952308563535	20.06400677550765
68-69	20.84829340356926	29.819587439833207	29.41163640939785	19.92048274719968
70-71	19.632990762040016	30.96947817550773	29.29673612153914	20.10079494091311
72-73	20.13903069939591	30.849443757534722	28.41495892502896	20.596566618040406
74-75	19.99830535103746	29.0897496127206	30.686946609293088	20.224998426948858
76-77	19.558063344356917	30.313004365554697	28.753406186510983	21.375526103577407
78-79	19.496724000359773	29.507158251261238	28.469909558244776	22.52620819013421
80-81	20.706631651560837	29.485454744082322	28.472021435235888	21.335892169120953
82-83	19.61592846496844	28.58780153846332	30.279922038427227	21.51634795814101
84-85	20.552115859945562	29.125379703664183	28.415808179634332	21.906696256755918
86-87	21.46577870663928	28.531827939472855	29.258365254369124	20.744028099518744
88-89	21.12717703522901	28.605809596345427	28.95466022674326	21.3123531416823
90-91	21.253754573911593	28.79733581109798	28.089868122340526	21.859041492649894
92-93	21.538486481603794	28.382301225204177	28.910981518289663	21.168230774902362
94-95	21.347674412769393	29.10273934793459	28.39656484232623	21.15302139696978
96-97	20.697280493741186	28.878420324670035	29.043909165271508	21.380390016317268
98-99	22.409435681895474	29.120187669826798	27.487804510854442	20.98257213742329
100	22.209359017366097	30.726301839755692	26.670995291655263	20.393343851222944
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	5.0
2	13.5
3	22.5
4	29.0
5	27.5
6	26.5
7	40.0
8	47.5
9	55.5
10	61.0
11	70.0
12	94.0
13	116.5
14	138.0
15	172.5
16	222.0
17	271.5
18	414.0
19	726.0
20	1187.5
21	1819.5
22	2849.5
23	4417.5
24	6697.0
25	10005.0
26	14320.0
27	19078.0
28	24340.5
29	30470.0
30	37677.5
31	45623.0
32	54577.5
33	65248.0
34	76808.0
35	89462.5
36	101908.5
37	110561.0
38	115743.0
39	120555.5
40	123642.5
41	123631.0
42	121546.5
43	119554.0
44	119764.5
45	117340.5
46	108754.5
47	98540.5
48	92866.0
49	91614.0
50	88833.5
51	81739.5
52	71348.5
53	60235.0
54	50840.0
55	42276.5
56	32973.0
57	23942.5
58	16845.5
59	11988.0
60	9182.5
61	7594.5
62	6304.5
63	5098.5
64	4080.5
65	3879.0
66	3875.0
67	3089.0
68	2178.0
69	2115.0
70	2647.0
71	2558.0
72	1585.0
73	689.0
74	318.0
75	178.5
76	122.5
77	91.0
78	69.5
79	61.0
80	46.0
81	31.5
82	27.0
83	25.5
84	20.0
85	17.0
86	19.5
87	18.0
88	15.5
89	13.5
90	12.5
91	10.5
92	11.5
93	10.5
94	12.0
95	13.5
96	13.0
97	18.0
98	41.0
99	102.0
100	103.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.367272892912467
2	0.006330807058232231
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.4742233856152485E-4
30-31	0.010152452782409003
32-33	0.0
34-35	0.0
36-37	0.0
38-39	6.369409540294622E-4
40-41	0.0012545806670277286
42-43	9.65062051559791E-5
44-45	0.0
46-47	0.002200341477556324
48-49	1.9301241031195827E-5
50-51	0.0
52-53	0.0015827017645580576
54-55	0.01810456408726168
56-57	0.01187026323418543
58-59	0.014109207193804148
60-61	1.3510868721837075E-4
62-63	0.011503539654592712
64-65	1.7371116928076243E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.3161489237434987E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2590507.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.18444273758597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.80735881138041	62.79763737394961
2	8.698328864798373	12.731647014370552
3	2.4753093396514902	5.434624038766087
4	1.0380553010157438	3.03877994942537
5	0.5948574613805305	2.176715590971459
6	0.33082557898397585	1.4526771384768902
7	0.22256927244579278	1.1402025722118703
8	0.15578433895300142	0.9120792026814891
9	0.12290606361599994	0.8095330597326521
>10	0.5166657572039719	6.42327332923999
>50	0.025508210654302078	1.2715958702867391
>100	0.01129272933557991	1.509956947350297
>500	4.306164646448314E-4	0.21528292259934959
>1k	1.0765411615328908E-4	0.08599498993752164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.8602482062391647E-5	0.0
2	0.0	0.0	0.0	3.8602482062391647E-5	0.0
3	0.0	0.0	0.0	3.8602482062391647E-5	0.0
4	0.0	0.0	0.0	3.8602482062391647E-5	0.0
5	0.0	0.0	0.0	3.8602482062391647E-5	0.0
6	0.0	0.0	0.0	3.8602482062391647E-5	0.0
7	0.0	0.0	0.0	3.8602482062391647E-5	0.0
8	0.0	0.0	0.0	3.8602482062391647E-5	0.0
9	0.0	0.0	0.0	3.8602482062391647E-5	0.0
10-11	0.0	0.0	0.0	3.8602482062391647E-5	0.0
12-13	0.0	0.0	0.0	3.8602482062391647E-5	0.0
14-15	0.0	0.0	0.0	3.8602482062391647E-5	0.0
16-17	0.0	0.0	0.0	3.8602482062391647E-5	0.0
18-19	0.0	0.0	0.0	3.8602482062391647E-5	0.0
20-21	0.0	0.0	0.0	3.8602482062391647E-5	0.0
22-23	0.0	0.0	0.0	3.8602482062391647E-5	0.0
24-25	0.0	0.0	0.0	3.8602482062391647E-5	0.0
26-27	0.0	0.0	0.0	3.8602482062391647E-5	0.0
28-29	0.0	0.0	0.0	3.8602482062391647E-5	0.0
30-31	0.0	0.0	0.0	3.8602482062391647E-5	0.0
32-33	0.0	0.0	0.0	3.8602482062391647E-5	0.0
34-35	0.0	0.0	0.0	3.8602482062391647E-5	0.0
36-37	0.0	0.0	0.0	3.8602482062391647E-5	0.0
38-39	0.0	0.0	0.0	3.8602482062391647E-5	0.0
40-41	0.0	0.0	0.0	3.8602482062391647E-5	0.0
42-43	1.9301241031195823E-5	0.0	0.0	3.8602482062391647E-5	0.0
44-45	3.8602482062391647E-5	0.0	0.0	3.8602482062391647E-5	0.0
46-47	3.8602482062391647E-5	0.0	0.0	3.8602482062391647E-5	0.0
48-49	3.8602482062391647E-5	0.0	0.0	3.8602482062391647E-5	0.0
50-51	3.8602482062391647E-5	0.0	0.0	7.720496412478329E-5	0.0
52-53	3.8602482062391647E-5	0.0	0.0	7.720496412478329E-5	0.0
54-55	5.7903723093587466E-5	0.0	0.0	7.720496412478329E-5	0.0
56-57	1.5440992824956659E-4	0.0	2.1231365134315405E-4	7.720496412478329E-5	0.0
58-59	2.7021737443674155E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
60-61	4.246273026863081E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
62-63	4.4392854371750397E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
64-65	4.825310257798956E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
66-67	5.597359899046789E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
68-69	6.56242195060658E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
70-71	7.72049641247833E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
72-73	8.878570874350079E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
74-75	9.264595694973996E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
76-77	9.84363292590987E-4	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
78-79	0.0011966769439341412	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
80-81	0.0013896893542460994	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
82-83	0.0015827017645580576	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
84-85	0.0018336178979636033	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
86-87	0.0019301241031195824	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
88	0.0019301241031195824	0.0	4.246273026863081E-4	7.720496412478329E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10830	0.0	80.48377	8
CACCGGC	10605	0.0	78.29645	9
CTTCACC	11925	0.0	75.88808	6
CCTTCAC	13125	0.0	71.596146	5
GTGTGGC	4450	0.0	69.72144	7
CGTAGTG	2285	0.0	66.76088	4
TTCACCG	13420	0.0	66.59464	7
CAGCTGC	80	0.0	66.15936	1
CGGGCCC	675	0.0	66.060936	7
TGCAGCC	65	1.0913936E-11	64.99113	3
GCGTAGT	1520	0.0	64.23099	3
GCAGCCT	75	1.2732926E-11	63.512993	1
TGTGGCA	4885	0.0	62.840275	8
GCCTTCA	3815	0.0	62.255943	4
CCGCGTA	215	0.0	61.543594	1
GGCGCGC	360	0.0	61.28021	6
GGCCCGA	725	0.0	60.85759	9
ACGGGCC	710	0.0	60.821114	6
CGGGGCA	1295	0.0	60.53013	94
GGGCCCG	740	0.0	60.258286	8
>>END_MODULE