FastQCFastQC Report
Tue 7 Mar 2017
1NF_8A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_8A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2772580
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA162530.5862049066212697No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66920.24136363964249902No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA66020.23811756558872965No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG60800.21929033607686704No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60380.21777550151844133No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG54030.1948726456946238No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG52930.19090522185112785No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG52780.19036420950883293No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45580.1643956170786776No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA44610.16089707059850392No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT44130.15916583110316024No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA43400.15653290437065837No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC40500.14607333241962359No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA37660.13583016540550677No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC34240.12349508400118302No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34160.12320654408529239No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA33250.11992440254203666No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC31920.11512742644035519No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC29170.10520886683161532No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG28740.10365796478370326No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC28280.10199886026733224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC4800.089.999881
GCAGCCG158.2055025E-489.999883
GCAGCAC355.0749804E-1089.999883
GCTGCAA450.089.999873
AGCTGCA1650.089.999872
GCAGCTT256.377795E-789.999873
GCAGCAT256.377795E-789.999873
AGCTGCC2000.087.749882
CTGCTGC1000.085.499881
CAGCAGC3650.085.068371
CAGCCCT166900.083.879451
AGCAGCA1400.083.571312
GCTGCCC1300.083.0768053
CTGCAGC650.083.0768051
AGCAGCG1250.082.799882
CAGCGTA59350.082.569391
GCTGCAG550.081.818073
AGCTGCG550.081.818072
AGCCCTT142950.081.248572
AGCAGCC501.8189894E-1280.9998862
Produced by FastQC (version 0.11.5)