##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_8A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2772580
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.716726658924179	12.0	12.0	12.0	2.0	27.0
2	14.17875228126871	12.0	12.0	12.0	12.0	22.0
3	13.800402513182668	12.0	12.0	12.0	12.0	22.0
4	13.743861313289427	12.0	12.0	12.0	12.0	22.0
5	22.429902834183324	22.0	22.0	22.0	22.0	27.0
6	26.814817606705667	27.0	22.0	32.0	22.0	32.0
7	34.81945372180424	37.0	37.0	37.0	27.0	41.0
8	37.38043338695367	41.0	37.0	41.0	27.0	41.0
9	38.41892605443306	41.0	37.0	41.0	32.0	41.0
10-11	38.82536446198126	41.0	41.0	41.0	34.5	41.0
12-13	38.99909470601389	41.0	41.0	41.0	37.0	41.0
14-15	38.95417607427017	41.0	41.0	41.0	37.0	41.0
16-17	38.8579444055717	41.0	41.0	41.0	37.0	41.0
18-19	38.77610240281615	41.0	41.0	41.0	34.5	41.0
20-21	38.78501810587973	41.0	41.0	41.0	34.5	41.0
22-23	38.797247689877295	41.0	41.0	41.0	34.5	41.0
24-25	38.770205007610244	41.0	41.0	41.0	32.0	41.0
26-27	38.735727012385574	41.0	41.0	41.0	32.0	41.0
28-29	38.68827211478118	41.0	41.0	41.0	32.0	41.0
30-31	38.59835406011729	41.0	41.0	41.0	32.0	41.0
32-33	38.63846832192398	41.0	41.0	41.0	32.0	41.0
34-35	38.6087207582829	41.0	41.0	41.0	32.0	41.0
36-37	38.534819193675204	41.0	41.0	41.0	32.0	41.0
38-39	38.423812658246106	41.0	41.0	41.0	32.0	41.0
40-41	38.47677434014528	41.0	41.0	41.0	32.0	41.0
42-43	38.23061192102662	41.0	41.0	41.0	32.0	41.0
44-45	38.34367881179263	41.0	41.0	41.0	32.0	41.0
46-47	38.3486236646012	41.0	41.0	41.0	32.0	41.0
48-49	38.30389979730071	41.0	41.0	41.0	32.0	41.0
50-51	38.18550231192607	41.0	41.0	41.0	32.0	41.0
52-53	38.16308005539966	41.0	39.0	41.0	32.0	41.0
54-55	38.01909448960895	41.0	37.0	41.0	32.0	41.0
56-57	37.80344408457105	41.0	37.0	41.0	27.0	41.0
58-59	37.76075604671462	41.0	37.0	41.0	27.0	41.0
60-61	37.81443060254348	41.0	37.0	41.0	27.0	41.0
62-63	37.80245439265954	41.0	37.0	41.0	27.0	41.0
64-65	37.788136861695605	41.0	37.0	41.0	27.0	41.0
66-67	37.654491664803174	41.0	37.0	41.0	27.0	41.0
68-69	37.646542390120395	41.0	37.0	41.0	27.0	41.0
70-71	37.63776464520411	41.0	37.0	41.0	27.0	41.0
72-73	37.50909026971269	41.0	37.0	41.0	27.0	41.0
74-75	37.399583961508775	41.0	37.0	41.0	27.0	41.0
76-77	36.29808589833296	39.0	34.5	41.0	24.5	41.0
78-79	36.85444513774174	41.0	37.0	41.0	27.0	41.0
80-81	37.543256641828194	41.0	37.0	41.0	27.0	41.0
82-83	37.7326428813596	41.0	37.0	41.0	27.0	41.0
84-85	37.66592289492098	41.0	37.0	41.0	27.0	41.0
86-87	37.536215726868114	41.0	37.0	41.0	27.0	41.0
88-89	37.432981194410985	41.0	37.0	41.0	27.0	41.0
90-91	37.24847362384494	41.0	37.0	41.0	27.0	41.0
92-93	37.14358233125825	41.0	37.0	41.0	27.0	41.0
94-95	36.98356963550195	41.0	37.0	41.0	27.0	41.0
96-97	36.90589991993017	41.0	37.0	41.0	27.0	41.0
98-99	36.776164438898064	41.0	37.0	41.0	27.0	41.0
100	36.638518275396926	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.4342548344496695
1101	2	-0.6537500242147427
1101	3	-0.018052851342220677
1101	4	-0.054639327986274466
1101	5	-1.1314028530783595
1101	6	-0.9812417796360933
1101	7	-0.599982830856618
1101	8	-0.24312372780930502
1101	9	-0.25189592866909294
1101	10-11	-0.12455954239120359
1101	12-13	-0.06548976937839512
1101	14-15	-0.03846219363789771
1101	16-17	-0.22878890039388722
1101	18-19	-0.10964544224103889
1101	20-21	-0.08959555769511951
1101	22-23	-0.03166095370077926
1101	24-25	-0.0943276212717592
1101	26-27	-0.1243373631703264
1101	28-29	-0.12581772022215176
1101	30-31	-0.15158266027446388
1101	32-33	-0.2536663566421211
1101	34-35	-0.07611814774496395
1101	36-37	-0.14714933081496184
1101	38-39	-0.15558415743949183
1101	40-41	-0.021664099870420728
1101	42-43	-0.06861298709472408
1101	44-45	-0.12794219759511094
1101	46-47	-0.03339007769459812
1101	48-49	-0.12471200487429712
1101	50-51	-0.06874151635269499
1101	52-53	-0.10338913750102563
1101	54-55	-0.22263501496566818
1101	56-57	0.1279045532267702
1101	58-59	0.002532467018404816
1101	60-61	0.03839277263792695
1101	62-63	-0.1566882135051273
1101	64-65	-0.07556906748249048
1101	66-67	-0.13507815370974896
1101	68-69	-0.20212214434688036
1101	70-71	-0.4174441225163079
1101	72-73	-0.03487469496771922
1101	74-75	-0.0433125830515948
1101	76-77	-0.5671748367784417
1101	78-79	-0.18808978978276514
1101	80-81	-0.14561766100978701
1101	82-83	-0.1316544991358981
1101	84-85	-0.24416176146264945
1101	86-87	-0.045874728695117994
1101	88-89	-0.1529642757873546
1101	90-91	-0.20071134884489794
1101	92-93	-0.08257822662606884
1101	94-95	-0.18060558048860997
1101	96-97	-0.22856431245177333
1101	98-99	-0.4499881707960469
1101	100	-0.5831223387028501
1102	1	1.4491621766969143
1102	2	0.4722410618748665
1102	3	-0.1261692013304927
1102	4	0.015311410929982117
1102	5	-0.7905982553772084
1102	6	-0.26063188050402886
1102	7	-0.2256877335073355
1102	8	-0.3936914031553087
1102	9	-0.35398600607936714
1102	10-11	-0.31849691044187267
1102	12-13	-0.35888878415672565
1102	14-15	-0.3804068335628301
1102	16-17	-0.3657358860847211
1102	18-19	-0.3217578044272926
1102	20-21	-0.143924668649845
1102	22-23	-0.22025232128229533
1102	24-25	-0.1682122095898393
1102	26-27	-0.2387591318778064
1102	28-29	-0.524732803496839
1102	30-31	-0.46448202691767904
1102	32-33	-0.3218847474467168
1102	34-35	-0.18791383153572383
1102	36-37	-0.39874679738780117
1102	38-39	-0.33619640233744974
1102	40-41	-0.3936052211465153
1102	42-43	-0.29110301758968404
1102	44-45	-0.10257156658408917
1102	46-47	-0.5541407227802182
1102	48-49	-0.47865171869085543
1102	50-51	-0.5193197476452198
1102	52-53	-0.4741444741184324
1102	54-55	-0.367169380435989
1102	56-57	-0.26657937827685885
1102	58-59	-0.3321121494488679
1102	60-61	-0.2565978442984971
1102	62-63	-0.3641958372442744
1102	64-65	-0.4254904843250884
1102	66-67	-0.22940609178153437
1102	68-69	-0.5563761907930527
1102	70-71	-0.7389688703464756
1102	72-73	-0.4569401418361281
1102	74-75	-0.6729830029436883
1102	76-77	-0.4302419256829708
1102	78-79	-0.530812051101087
1102	80-81	-0.641524299527255
1102	82-83	-0.43572205719360113
1102	84-85	-0.5729079495930733
1102	86-87	-0.4371684185848679
1102	88-89	-0.46597037477867786
1102	90-91	-0.4184629322368707
1102	92-93	-0.38677362892491374
1102	94-95	-0.3234310555062052
1102	96-97	-0.348488075060267
1102	98-99	-0.4454409036133171
1102	100	-0.7085809359348474
1103	1	1.6094114091332514
1103	2	0.6487987646019739
1103	3	0.5242913368670887
1103	4	0.972032860571785
1103	5	2.8088117741213168
1103	6	-0.07699573578065255
1103	7	-0.48266443569263373
1103	8	-0.34320437648623425
1103	9	-0.2959148484127567
1103	10-11	-0.34520451383721706
1103	12-13	-0.4235916918684879
1103	14-15	-0.5445980321661636
1103	16-17	-0.48591468326731047
1103	18-19	-0.44664974350380504
1103	20-21	-0.406608432420839
1103	22-23	-0.22341408156162856
1103	24-25	-0.346612667117995
1103	26-27	-0.281311657312763
1103	28-29	-0.3153293961161907
1103	30-31	-0.3837927246480959
1103	32-33	-0.3600263403670638
1103	34-35	-0.4432494525097752
1103	36-37	-0.3471653152374188
1103	38-39	-0.25809153207076463
1103	40-41	-0.34566289377308834
1103	42-43	-0.1415364653050446
1103	44-45	-0.5273148090138022
1103	46-47	-0.5131516191741738
1103	48-49	-0.329227240935154
1103	50-51	-0.29846128036449926
1103	52-53	-0.3175368045019127
1103	54-55	-0.4207001732841036
1103	56-57	-0.4403907081185352
1103	58-59	-0.6849066831915707
1103	60-61	-0.6182563838205013
1103	62-63	-0.37328258698842376
1103	64-65	-0.4619434371876494
1103	66-67	-0.6304572024913071
1103	68-69	-0.6628676723414202
1103	70-71	-0.7639210104945704
1103	72-73	-0.49293556655468507
1103	74-75	-0.4722563760097316
1103	76-77	-0.6714459471145489
1103	78-79	-0.817983202747584
1103	80-81	-0.546957446237279
1103	82-83	-0.5431189628585287
1103	84-85	-0.66949816089884
1103	86-87	-0.5810568057981556
1103	88-89	-0.40437926045683525
1103	90-91	-0.7834289186314365
1103	92-93	-0.5094622087816418
1103	94-95	-0.7391176487275644
1103	96-97	-0.4468915426419855
1103	98-99	-0.5171536240997412
1103	100	-0.64436084081715
1104	1	1.591208448425533
1104	2	-0.4553938560048003
1104	3	-0.0620616141887087
1104	4	0.09696968784949789
1104	5	-0.963924645471522
1104	6	-0.3628216458390803
1104	7	-0.04956454060928195
1104	8	0.0794939730607922
1104	9	-0.03966375713117998
1104	10-11	-0.07888854218570174
1104	12-13	-0.020223043255263917
1104	14-15	-0.03545994107557959
1104	16-17	-3.9000532657951226E-4
1104	18-19	-0.1965743458676812
1104	20-21	0.046231306255307913
1104	22-23	-0.08223412007714614
1104	24-25	-0.17388756357104995
1104	26-27	0.008047060830136843
1104	28-29	-0.1071804605669584
1104	30-31	-0.042990801022703806
1104	32-33	-0.11093815252684891
1104	34-35	-0.1564913767700986
1104	36-37	-0.2345373760737104
1104	38-39	-0.1459648783791465
1104	40-41	-0.0871747072641682
1104	42-43	0.01625888221276739
1104	44-45	0.013330021268551207
1104	46-47	-0.1276500341596929
1104	48-49	-0.1518781757378349
1104	50-51	-0.0919652043698278
1104	52-53	-0.06531903871947975
1104	54-55	-0.1524389682225049
1104	56-57	-0.1499848717014487
1104	58-59	-0.236821731945291
1104	60-61	-0.15850566453473647
1104	62-63	-0.13865000773385816
1104	64-65	-0.267192239146091
1104	66-67	-0.6898549225896744
1104	68-69	-0.41028738345966786
1104	70-71	-0.22402503619069591
1104	72-73	-0.19909651349779267
1104	74-75	-0.18428502557534188
1104	76-77	-0.2911948025809963
1104	78-79	-0.2486867586998045
1104	80-81	-0.22455510601754014
1104	82-83	-0.23916945802513823
1104	84-85	-0.3634300976989806
1104	86-87	-0.4702250969062547
1104	88-89	-0.28767882486587837
1104	90-91	-0.4614072788698209
1104	92-93	-0.5397817872307655
1104	94-95	-0.2819606593282913
1104	96-97	-0.3944017309235832
1104	98-99	-0.5270538841639762
1104	100	-0.37286739413902126
1105	1	1.6197657022667542
1105	2	0.21745735879266626
1105	3	0.080702616320389
1105	4	-0.19608376382674209
1105	5	-1.088254730300914
1105	6	0.4689868545353342
1105	7	0.3236088799385257
1105	8	0.2647047340178261
1105	9	0.13350652902415305
1105	10-11	0.040693672683758564
1105	12-13	-0.012781799645871672
1105	14-15	0.01735065395454427
1105	16-17	0.11627918354652422
1105	18-19	0.0753638301999544
1105	20-21	0.17486334474172338
1105	22-23	0.12012984851428143
1105	24-25	0.03663121266418301
1105	26-27	0.059948926920078804
1105	28-29	0.12460296466774423
1105	30-31	0.13051398357841748
1105	32-33	0.1086852991934606
1105	34-35	0.0505308377467415
1105	36-37	0.02036317308164115
1105	38-39	0.07950250116590496
1105	40-41	-0.008204913502297018
1105	42-43	0.37586430138143356
1105	44-45	0.0928821263804025
1105	46-47	-0.22220388032860683
1105	48-49	-0.038020389980417235
1105	50-51	0.08097489983892814
1105	52-53	-0.16353987919702462
1105	54-55	-0.14956291826224088
1105	56-57	0.11370079183229365
1105	58-59	-0.036509076297001286
1105	60-61	0.04436054147418389
1105	62-63	-0.11661841207831714
1105	64-65	-0.12616318871518928
1105	66-67	-0.06769907765787053
1105	68-69	0.0012086375760205215
1105	70-71	-0.12836435768288368
1105	72-73	-0.0060906654071004596
1105	74-75	-0.11569594785563453
1105	76-77	-0.2494356346054758
1105	78-79	-0.0836857277785299
1105	80-81	-0.04569271417243925
1105	82-83	-0.11198927992307972
1105	84-85	-0.05333338699899315
1105	86-87	-0.15649053195257778
1105	88-89	-0.16018839088475545
1105	90-91	0.003005122941665661
1105	92-93	-0.118452099587671
1105	94-95	-0.1220033066557562
1105	96-97	-0.08968295204370946
1105	98-99	-0.32504326934800076
1105	100	-0.12250191908022146
1106	1	1.4799973888902294
1106	2	-0.5990785218085861
1106	3	0.016509900477373662
1106	4	-0.24978089586636187
1106	5	-1.1223902302185103
1106	6	-0.9482092742825756
1106	7	-0.2948931213289967
1106	8	-0.30683998423469916
1106	9	-0.12697751169098836
1106	10-11	-0.07246564510246856
1106	12-13	-0.15255717854260809
1106	14-15	0.026560395236558065
1106	16-17	0.0016041473603820577
1106	18-19	0.04887823474174269
1106	20-21	-0.09007041271999583
1106	22-23	0.01550198206970066
1106	24-25	-0.06459123735260164
1106	26-27	-0.10441035445408176
1106	28-29	-0.13540694677828924
1106	30-31	0.05330468988889692
1106	32-33	-0.03545821433856844
1106	34-35	-0.1692632828531444
1106	36-37	-0.12006979213808933
1106	38-39	-0.17953442242114193
1106	40-41	-0.1389067677741309
1106	42-43	0.07710625552029882
1106	44-45	-0.012553263298649142
1106	46-47	-0.18943120423981696
1106	48-49	-0.12353528483643572
1106	50-51	-0.12835025589417626
1106	52-53	-0.27482527314003846
1106	54-55	-0.1940943452853574
1106	56-57	-0.17346856852287829
1106	58-59	-0.19767277076221035
1106	60-61	-0.16798307021928593
1106	62-63	-0.1394845715720905
1106	64-65	-0.3484631042740105
1106	66-67	-0.22542695752692765
1106	68-69	-0.3414174979804159
1106	70-71	-0.4550804205515533
1106	72-73	-0.35997299623348766
1106	74-75	-0.32447771579010265
1106	76-77	-0.4700123026474543
1106	78-79	-0.3267503856685039
1106	80-81	-0.4177920100358463
1106	82-83	-0.33193152997579034
1106	84-85	-0.4908794463858399
1106	86-87	-0.39691375332699863
1106	88-89	-0.2830878569121751
1106	90-91	-0.588439304359305
1106	92-93	-0.4749327343934411
1106	94-95	-0.3672190868214642
1106	96-97	-0.08091201242974932
1106	98-99	-0.609096735289647
1106	100	-0.5449868362484978
1107	1	1.2885241283741369
1107	2	-0.337658070191754
1107	3	-0.16378355741775508
1107	4	-0.20447512437379523
1107	5	-1.2997115558698198
1107	6	-0.48124177963609327
1107	7	-0.4866823382457852
1107	8	-0.3847493435728495
1107	9	-0.11191234903034086
1107	10-11	-0.16807349969826646
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2223	1	1.5358854236880184
2223	2	-0.7344308376541768
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2223	4	-0.10521285646928646
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2223	9	0.2616903413826961
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2223	30-31	0.20933174859252546
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2223	34-35	0.28537551304826536
2223	36-37	0.2113434694881775
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2223	66-67	0.5492394144978974
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2223	74-75	0.5926606391285247
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2223	94-95	0.7183085441798269
2223	96-97	0.6398596486446024
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2223	100	0.9611080358076975
2224	1	1.5988299223409168
2224	2	-0.7025076070693395
2224	3	0.08019214464617264
2224	4	-0.19368921688806573
2224	5	-0.9565248577400887
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2224	7	0.5601261877463415
2224	8	0.3313673426762307
2224	9	0.38527985447979773
2224	10-11	0.3889579804237826
2224	12-13	0.30051535944237884
2224	14-15	0.2982990459124082
2224	16-17	0.260114481229607
2224	18-19	0.3152805250874948
2224	20-21	0.4281938503518461
2224	22-23	0.42679538473578305
2224	24-25	0.4074515245414432
2224	26-27	0.3559339303251008
2224	28-29	0.19278821210076558
2224	30-31	0.24365930552421133
2224	32-33	0.34438601335460106
2224	34-35	0.28887924126670583
2224	36-37	0.20086949979027935
2224	38-39	0.33972147580061574
2224	40-41	0.36629506177928306
2224	42-43	0.5440307402682336
2224	44-45	0.4451298040160907
2224	46-47	0.4435313768227118
2224	48-49	0.3084480880716427
2224	50-51	0.4617100370213407
2224	52-53	0.5086481747029836
2224	54-55	0.42612030459503814
2224	56-57	0.5954852462145652
2224	58-59	0.5699825613879241
2224	60-61	0.5333499734222755
2224	62-63	0.678284777854337
2224	64-65	0.5970494881161486
2224	66-67	0.47199532855487547
2224	68-69	0.5632222502613615
2224	70-71	0.7549122682056293
2224	72-73	0.5774321624191785
2224	74-75	0.4796769887397616
2224	76-77	0.7512726649715091
2224	78-79	0.4511899546552769
2224	80-81	0.4937415447249407
2224	82-83	0.39550748776061084
2224	84-85	0.4260857477660096
2224	86-87	0.5836024930558565
2224	88-89	0.5731657638957302
2224	90-91	0.652324367900917
2224	92-93	0.5859288423952052
2224	94-95	0.6075400240962381
2224	96-97	0.5430815515918326
2224	98-99	0.4304106051883707
2224	100	0.6454882218920872
2225	1	1.6780937493259653
2225	2	1.5347460638756658
2225	3	0.7114458446879155
2225	4	0.9312377814782007
2225	5	3.176268491321469
2225	6	-0.034373377102667746
2225	7	0.1745683689336417
2225	8	0.3228992330991929
2225	9	0.03200038847845832
2225	10-11	-0.015479703559122981
2225	12-13	0.11509280848033399
2225	14-15	0.12922902077890797
2225	16-17	0.047185282382670835
2225	18-19	0.17995488064673992
2225	20-21	0.18582323050981842
2225	22-23	0.18343715450802023
2225	24-25	0.3767297672008709
2225	26-27	0.2514129369497269
2225	28-29	0.15657972151316812
2225	30-31	0.29100816916075445
2225	32-33	0.12044018252527167
2225	34-35	0.08002534413454754
2225	36-37	0.06919237828246594
2225	38-39	0.1260284876185338
2225	40-41	0.05550642351134627
2225	42-43	0.33976933134989196
2225	44-45	0.3299254322154326
2225	46-47	0.24391049770797935
2225	48-49	0.29238069427432833
2225	50-51	0.3292676873287732
2225	52-53	0.18499518174391483
2225	54-55	0.3003776383715362
2225	56-57	0.49007528358788477
2225	58-59	0.34358612986285664
2225	60-61	0.3524097587427164
2225	62-63	0.3210942788021427
2225	64-65	0.2709160782999547
2225	66-67	0.2684970694829687
2225	68-69	0.4366730288948233
2225	70-71	0.36754573549586667
2225	72-73	0.4725196420774438
2225	74-75	0.3497981095012861
2225	76-77	0.5478453092109916
2225	78-79	0.25978418739431675
2225	80-81	0.2028734595763737
2225	82-83	0.2118449696171183
2225	84-85	0.21114967345614133
2225	86-87	0.2980576718513106
2225	88-89	0.15060025347263206
2225	90-91	0.45846784007204633
2225	92-93	0.29557130905027407
2225	94-95	0.4645921537775095
2225	96-97	0.28127583050196137
2225	98-99	0.6646111171950082
2225	100	0.5718672433368681
2226	1	1.4413502062222108
2226	2	1.3753068325330684
2226	3	0.855313841461351
2226	4	0.9799182500204164
2226	5	3.2604576955572355
2226	6	-0.3227244404927845
2226	7	0.22261042901405403
2226	8	0.3253424947294832
2226	9	0.4099109694787799
2226	10-11	0.2145500969394476
2226	12-13	0.2616724609974739
2226	14-15	0.27012936633632734
2226	16-17	0.2100190671724036
2226	18-19	0.2519878250517209
2226	20-21	0.2615006460689955
2226	22-23	0.1344527977033323
2226	24-25	0.18678477340748145
2226	26-27	0.2707040069621698
2226	28-29	0.2850836139571413
2226	30-31	0.27454666353784773
2226	32-33	0.2531768737995961
2226	34-35	0.21774920646198126
2226	36-37	0.35517505811017
2226	38-39	0.27546080003317286
2226	40-41	0.18317766211615094
2226	42-43	0.37085588504792355
2226	44-45	0.19255237849819906
2226	46-47	0.398878924933058
2226	48-49	0.3335410223180588
2226	50-51	0.20167594589343452
2226	52-53	0.40268420145872597
2226	54-55	0.4540818904247246
2226	56-57	0.43061456126736175
2226	58-59	0.48209280835441604
2226	60-61	0.4166259677050377
2226	62-63	0.4541000935217099
2226	64-65	0.33487337446265286
2226	66-67	0.4294241377586445
2226	68-69	0.5897126165403321
2226	70-71	0.691122796940121
2226	72-73	0.5558650093859683
2226	74-75	0.5739166755182197
2226	76-77	0.7331065339925189
2226	78-79	0.4815684053342224
2226	80-81	0.2805076724352773
2226	82-83	0.39934816044387844
2226	84-85	0.3797857791742487
2226	86-87	0.3985476855967818
2226	88-89	0.41705993576596256
2226	90-91	0.47642180172961446
2226	92-93	0.5240092212746958
2226	94-95	0.6307570401451841
2226	96-97	0.6819449681448688
2226	98-99	0.605793422437884
2226	100	0.6696599448646197
2227	1	1.1457396593646223
2227	2	1.2483013424464247
2227	3	0.6207109861511988
2227	4	-0.011368926276672298
2227	5	3.2610536015378067
2227	6	0.12554752905884214
2227	7	0.20930331554386328
2227	8	0.36384431926763483
2227	9	0.19072512239116435
2227	10-11	0.08751747617401406
2227	12-13	0.08783142545822642
2227	14-15	0.030658492088960543
2227	16-17	0.02068568732933329
2227	18-19	-0.03615548694527604
2227	20-21	-0.061247846724903354
2227	22-23	0.057353706774861735
2227	24-25	0.2675360897298873
2227	26-27	0.3226892943826485
2227	28-29	0.08078911819049495
2227	30-31	0.11560666884771109
2227	32-33	0.31774159112698896
2227	34-35	0.06321431350219342
2227	36-37	0.0640379241563167
2227	38-39	0.04597086648395532
2227	40-41	0.21770096640027248
2227	42-43	0.35192012468158396
2227	44-45	0.2920674660624627
2227	46-47	0.2228638015175406
2227	48-49	0.26919989085247664
2227	50-51	0.27265173951423805
2227	52-53	0.198096355227662
2227	54-55	0.1377585237400325
2227	56-57	0.2589718374247312
2227	58-59	0.21769223163371265
2227	60-61	0.1433328265344258
2227	62-63	0.31430902881697875
2227	64-65	0.37780476678971553
2227	66-67	0.3513524604431879
2227	68-69	0.34873187157065644
2227	70-71	0.5175363537527318
2227	72-73	0.3569758168918611
2227	74-75	0.2722244963239646
2227	76-77	0.4926327935827288
2227	78-79	0.2831392231885346
2227	80-81	0.2859461623416948
2227	82-83	0.08045198179998181
2227	84-85	0.1583551106549521
2227	86-87	0.06564820088589585
2227	88-89	0.3452905697905422
2227	90-91	0.24305775259208673
2227	92-93	0.1602427757230629
2227	94-95	0.4375473645699657
2227	96-97	0.20090277218772457
2227	98-99	0.3391191979392687
2227	100	0.47751514843191956
2228	1	1.4350861520218103
2228	2	1.2083670056813016
2228	3	-0.2543383184397019
2228	4	-0.17472670706512616
2228	5	3.1829808536857698
2228	6	0.1782014975305799
2228	7	0.2037016470955635
2228	8	0.46849343254719855
2228	9	0.32064057573615656
2228	10-11	0.3435097703666514
2228	12-13	0.2520254788005474
2228	14-15	0.21825602943182787
2228	16-17	0.21480191087397316
2228	18-19	0.20032933867367575
2228	20-21	0.2228447296710101
2228	22-23	0.30803968369173873
2228	24-25	0.3871597456438636
2228	26-27	0.32343529427975426
2228	28-29	0.30786986720107024
2228	30-31	0.3616801655578783
2228	32-33	0.22084211151669564
2228	34-35	0.36508464862097156
2228	36-37	0.45579672133390403
2228	38-39	0.304093261915348
2228	40-41	0.250333936356391
2228	42-43	0.5730624096325769
2228	44-45	0.40323074000162507
2228	46-47	0.37710571127727377
2228	48-49	0.4145033784734551
2228	50-51	0.443966852538459
2228	52-53	0.35278105355978084
2228	54-55	0.31738348491564494
2228	56-57	0.5681411574882205
2228	58-59	0.6882738257297305
2228	60-61	0.5072007073802354
2228	62-63	0.4993672606352604
2228	64-65	0.5574297279653209
2228	66-67	0.3965609593160835
2228	68-69	0.4993007084015133
2228	70-71	0.5974283695530715
2228	72-73	0.6460712610523913
2228	74-75	0.7011563349898111
2228	76-77	0.6069994368118472
2228	78-79	0.5872497016764839
2228	80-81	0.5430281594491575
2228	82-83	0.39190146055280195
2228	84-85	0.44948299961814797
2228	86-87	0.5139700822156996
2228	88-89	0.6731189383659384
2228	90-91	0.5845804117434881
2228	92-93	0.5780463062176011
2228	94-95	0.7903171869209942
2228	96-97	0.682071991260166
2228	98-99	0.7691066788605383
2228	100	0.9035358725567733
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	23.0
13	47.0
14	155.0
15	492.0
16	1868.0
17	4615.0
18	7647.0
19	9855.0
20	11711.0
21	13597.0
22	15366.0
23	17808.0
24	20164.0
25	22924.0
26	26036.0
27	29177.0
28	33173.0
29	37530.0
30	42669.0
31	49249.0
32	57765.0
33	68668.0
34	84652.0
35	108976.0
36	153129.0
37	254030.0
38	616037.0
39	1084296.0
40	921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.875143559561096	23.798361213959584	20.195245071376004	38.13125015510332
2	31.510441464604057	44.02176735891687	10.392770472455712	14.07502070402336
3	32.49659162224354	16.548521593605955	27.274920831860577	23.679965952289926
4	19.683399577289023	21.96048445851878	18.855073613746043	39.501042350446156
5	17.246571785124324	35.581912875372396	26.316860108635275	20.854655230867998
6	17.201487423266414	27.719236234842636	31.149543024908212	23.929733316982738
7	20.27054223863694	26.613623412128774	36.59659955709123	16.51923479214306
8	20.393207770379934	27.714727798656845	34.702262874290376	17.189801556672847
9	20.696896031854806	28.178483578472036	27.794364815442655	23.3302555742305
10-11	19.343896298754228	30.379429989396158	28.77386766116758	21.502806050682036
12-13	23.98578219564449	26.664208066133348	27.50411169380144	21.84589804442072
14-15	21.801859639757915	27.418469440016157	28.549816416478514	22.22985450374741
16-17	20.803764003202794	29.878164020515186	28.15877269546776	21.15929928081426
18-19	21.48212134546163	29.594024338341907	29.86222219016223	19.061632126034237
20-21	22.79907162282062	29.024338341905377	29.842853948308072	18.333736086965928
22-23	22.092329166336047	30.25490698194461	28.670570371278735	18.9821934804406
24-25	22.208628786184708	28.36787396576474	26.483870618701715	22.93962662934884
26-27	20.03581501705992	26.391105035742886	30.26334677448442	23.30973317271278
28-29	19.34023425876667	28.24185098689846	30.864088437643705	21.553826316691165
30-31	19.91117906864631	30.258705751627836	28.240774557189386	21.589340622536472
32-33	20.462547518917397	30.22489883069199	28.961454673985966	20.35109897640465
34-35	21.231506394765887	29.31996551948005	29.972498539266674	19.476029546487386
36-37	21.928582764068125	28.133940228956423	28.821927590908107	21.115549416067346
38-39	21.56828905019609	29.18003654749687	29.076431805808106	20.175242596498936
40-41	20.8127797868701	29.945849186006523	29.836707171723205	19.404663855400177
42-43	20.424994278791388	30.03033097169296	29.531282883424222	20.01339186609144
44-45	20.671684856703866	29.969703308831484	28.896010214313023	20.462601620151627
46-47	19.88442650489816	28.234659316438123	28.885456794473914	22.99545738418981
48-49	20.677221216340016	28.102850053019207	30.507397442093648	20.71253128854713
50-51	21.80595329981461	30.63392219521168	28.363618002005353	19.196506502968354
52-53	20.439949021631183	30.400135471029632	28.240010950214216	20.91990455712497
54-55	22.35182541735343	28.417099829692887	28.30296051551721	20.928114237436468
56-57	21.708965400569312	28.773605748758563	29.359404000778422	20.158024849893707
58-59	21.306189824557038	28.1114743063563	29.004155432173782	21.578180436912877
60-61	19.495732033794596	30.538432975717328	29.687454633754523	20.278380356733557
62-63	20.544901334478013	31.40425900308234	27.19667633993388	20.854163322505766
64-65	19.776251560821734	30.31660425784234	28.946976424107817	20.96016775722811
66-67	20.031360682108364	30.27802624270535	29.691785268594593	19.99882780659169
68-69	20.82690129770827	29.976520064344403	29.394805560164176	19.801773077783146
70-71	19.559579885882464	31.083178844253368	29.31469966601505	20.042541603849124
72-73	19.96117334756797	30.88738647757684	28.65610730799472	20.49533286686047
74-75	19.951182652980258	29.228354096184784	30.68515606402701	20.135307186807953
76-77	19.460502492263522	30.42925722612152	28.77080192456124	21.33943835705372
78-79	19.507101688679857	29.607784085580942	28.54671461238269	22.338399613356515
80-81	20.463172154703784	29.609413772607134	28.579955968745313	21.34745810394377
82-83	19.87214074977097	28.57809332823579	30.257972718550953	21.291793203442282
84-85	20.44431540298206	29.192448910401144	28.41593389550527	21.947301791111528
86-87	21.31601252263235	28.81545347654531	29.28557516825484	20.5829588325675
88-89	21.04060117291476	28.722796096054935	29.46955543212459	20.767047298905712
90-91	21.094702407144247	28.868292348642782	28.9998665502889	21.03713869392407
92-93	21.378842089317533	28.633258553405135	29.591625850291063	20.396273506986272
94-95	21.286797856148425	29.1655245294996	29.266279061379652	20.28139855297232
96-97	20.650098464246298	28.95645932669211	29.5482727279285	20.845169481133098
98-99	22.07449379278506	29.31542101580477	28.406935056878428	20.203150134531736
100	21.82923486427804	30.96433646639592	27.592098334403335	19.61433033492271
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.0
2	16.0
3	21.0
4	25.5
5	35.5
6	50.0
7	51.0
8	53.0
9	70.5
10	79.5
11	80.0
12	101.5
13	126.0
14	157.0
15	198.5
16	249.5
17	351.0
18	527.0
19	846.5
20	1369.5
21	2125.0
22	3137.5
23	4731.5
24	7277.5
25	10876.0
26	15609.0
27	21074.0
28	27259.5
29	34439.5
30	42728.5
31	51830.5
32	61552.5
33	72471.0
34	84853.0
35	98697.5
36	112032.5
37	121311.0
38	127450.0
39	131754.5
40	133741.0
41	133756.0
42	131851.5
43	129199.0
44	127906.5
45	125070.5
46	115937.0
47	105341.5
48	98472.5
49	93431.5
50	88992.0
51	83054.0
52	73508.0
53	62544.5
54	52245.5
55	42147.5
56	32268.0
57	23709.5
58	16803.5
59	12163.5
60	9587.5
61	7940.0
62	6453.0
63	4904.0
64	3716.0
65	3280.0
66	3378.5
67	3024.0
68	2183.5
69	2099.0
70	2443.0
71	2246.5
72	1449.5
73	651.0
74	294.5
75	160.5
76	115.0
77	100.0
78	77.0
79	59.5
80	47.5
81	37.5
82	31.5
83	22.0
84	19.5
85	21.0
86	18.0
87	13.0
88	15.5
89	17.0
90	14.5
91	18.0
92	14.0
93	9.5
94	13.5
95	18.0
96	17.5
97	16.5
98	45.0
99	102.0
100	100.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.344704210518723
2	0.006203608191648211
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.8033744743163407E-4
30-31	0.009395581011188136
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.475860029286801E-4
40-41	0.0016410707716278702
42-43	5.4101234229490224E-5
44-45	0.0
46-47	0.0021099481349501187
48-49	0.0
50-51	0.0
52-53	0.0011361259188192947
54-55	0.019079701938266885
56-57	0.010621875653723247
58-59	0.015346716776432059
60-61	2.344386816611243E-4
62-63	0.012082608977919483
64-65	7.213497897265363E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4426995794530726E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2772580.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.7463055369503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43470543091526	67.1029061753427
2	8.15406922758308	12.515893766190707
3	2.05069648590345	4.721501372120894
4	0.8094829935137304	2.484993165886796
5	0.43234378555099073	1.6590394131449027
6	0.25645275184270605	1.1809080749227205
7	0.18051650890113954	0.9697782602613326
8	0.12627176324245767	0.7752713057996086
9	0.09647631222951003	0.6663780481899773
>10	0.4317300772294109	5.673692450358278
>50	0.019224238043074136	0.9976587677146608
>100	0.007507797709998394	0.9901356739417716
>500	4.751157592236978E-4	0.22494258638916179
>1k	4.751157587463587E-5	0.03690093973640203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.6067489486326816E-5	0.0
3	0.0	0.0	0.0	3.6067489486326816E-5	0.0
4	0.0	0.0	0.0	3.6067489486326816E-5	0.0
5	0.0	0.0	0.0	3.6067489486326816E-5	0.0
6	0.0	0.0	0.0	3.6067489486326816E-5	0.0
7	0.0	0.0	0.0	3.6067489486326816E-5	0.0
8	0.0	0.0	0.0	3.6067489486326816E-5	0.0
9	0.0	0.0	0.0	3.6067489486326816E-5	0.0
10-11	0.0	0.0	0.0	3.6067489486326816E-5	0.0
12-13	0.0	0.0	0.0	3.6067489486326816E-5	0.0
14-15	0.0	0.0	0.0	3.6067489486326816E-5	0.0
16-17	0.0	0.0	0.0	3.6067489486326816E-5	0.0
18-19	0.0	0.0	0.0	3.6067489486326816E-5	0.0
20-21	0.0	0.0	0.0	7.213497897265363E-5	0.0
22-23	0.0	0.0	0.0	7.213497897265363E-5	0.0
24-25	0.0	0.0	0.0	7.213497897265363E-5	0.0
26-27	0.0	0.0	0.0	7.213497897265363E-5	0.0
28-29	0.0	0.0	0.0	7.213497897265363E-5	0.0
30-31	0.0	0.0	0.0	7.213497897265363E-5	0.0
32-33	0.0	0.0	0.0	7.213497897265363E-5	0.0
34-35	0.0	0.0	0.0	7.213497897265363E-5	0.0
36-37	0.0	0.0	0.0	7.213497897265363E-5	0.0
38-39	0.0	0.0	0.0	7.213497897265363E-5	0.0
40-41	3.6067489486326816E-5	1.8033744743163408E-5	0.0	7.213497897265363E-5	0.0
42-43	3.6067489486326816E-5	3.6067489486326816E-5	0.0	7.213497897265363E-5	0.0
44-45	3.6067489486326816E-5	3.6067489486326816E-5	0.0	7.213497897265363E-5	0.0
46-47	1.2623621320214385E-4	3.6067489486326816E-5	0.0	7.213497897265363E-5	0.0
48-49	1.4426995794530726E-4	3.6067489486326816E-5	0.0	7.213497897265363E-5	0.0
50-51	1.8033744743163407E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
52-53	2.885399158906145E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
54-55	3.2460740537694136E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
56-57	3.4264115012010474E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
58-59	4.3280987383592174E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
60-61	4.6887736332224857E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
62-63	5.229785975517388E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
64-65	5.590460870380657E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
66-67	6.311810660107192E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
68-69	6.852823002402095E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
70-71	7.934847686991899E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
72-73	9.016872371581704E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
74-75	9.197209819013337E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
76-77	9.918559608739874E-4	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
78-79	0.001063990939846641	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
80-81	0.0011721934083056215	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
82-83	0.001244328387278275	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
84-85	0.001370564600480419	3.6067489486326816E-5	0.0	1.0820246845898045E-4	0.0
86-87	0.0014066320899667457	7.213497897265363E-5	0.0	1.0820246845898045E-4	0.0
88	0.0014066320899667457	7.213497897265363E-5	0.0	1.0820246845898045E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11500	0.0	79.875465	8
CACCGGC	11020	0.0	79.010124	9
CTTCACC	12730	0.0	75.77113	6
CCTTCAC	14055	0.0	72.36832	5
GTGTGGC	4455	0.0	71.74966	7
CGGGGCA	1150	0.0	71.018555	94
CGGGCCC	960	0.0	70.895256	7
GGGCCCG	960	0.0	70.40632	8
GGCCCGA	990	0.0	67.798676	9
GCAGCCA	125	0.0	67.59617	2
TTCACCG	14280	0.0	66.297646	7
ACGGGCC	1030	0.0	65.62143	6
CGTAGTG	2085	0.0	65.28484	4
AGCTGCA	65	2.5465852E-10	65.17528	1
AGGCAGC	65	1.0913936E-11	64.99631	2
AACGGGC	1035	0.0	64.39741	5
ACCTTCA	6515	0.0	64.12036	4
GCCTTCA	4100	0.0	62.507076	4
TGTGGCA	5165	0.0	60.705296	8
CAGCGTA	1040	0.0	58.04673	1
>>END_MODULE