FastQCFastQC Report
Tue 7 Mar 2017
1NF_8A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_8A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2772580
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG115000.079.8754658
CACCGGC110200.079.0101249
CTTCACC127300.075.771136
CCTTCAC140550.072.368325
GTGTGGC44550.071.749667
CGGGGCA11500.071.01855594
CGGGCCC9600.070.8952567
GGGCCCG9600.070.406328
GGCCCGA9900.067.7986769
GCAGCCA1250.067.596172
TTCACCG142800.066.2976467
ACGGGCC10300.065.621436
CGTAGTG20850.065.284844
AGCTGCA652.5465852E-1065.175281
AGGCAGC651.0913936E-1164.996312
AACGGGC10350.064.397415
ACCTTCA65150.064.120364
GCCTTCA41000.062.5070764
TGTGGCA51650.060.7052968
CAGCGTA10400.058.046731
Produced by FastQC (version 0.11.5)