FastQCFastQC Report
Tue 7 Mar 2017
1NF_9A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_9A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3380498
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA182450.5397133795079896No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG79100.2339891933082049No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA73750.21816312271150584No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT69110.20443733438091075No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG69040.20423026429833713No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG62440.1847065136556803No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61420.18168920673817882No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA60310.17840566685736836No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA56600.16743095248096584No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC52840.15630833090272497No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50900.1505695314713986No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT50140.1483213420034563No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA49870.14752264311352942No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC47010.13906235116837815No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG46540.13767202347109803No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA43430.12847219551675523No Hit
CTGCAGGTGGAGATAGAGGTGATGCAGGTGGAGATAGCGGTGATGCAGGT43280.1280284739112403No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA39640.1172608296174114No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA39040.11548594319535169No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38150.1128531950026298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT600.089.999893
GCTGCCG355.0749804E-1089.999893
GCAGCTT256.378086E-789.999893
GCAGCTA650.089.999893
GCAGCAT600.089.999893
CAGCAGC6150.088.536481
CAGCTGC6450.087.9068761
GCTGCCC1850.087.567473
AGCAGCG1400.086.7856142
AGCAGCA2450.086.326442
AGCTGCA1900.085.263062
AGCTGCC2550.084.705792
GCTGCAT850.084.705783
CAGCCCT193450.083.835521
TGCTGCA650.083.076832
AGCAGCT1300.083.076832
AGCAGCC1250.082.7999042
CAGCGTA73150.082.433261
GCAGCAA1150.082.173823
TGCTGCC550.081.818092
Produced by FastQC (version 0.11.5)