FastQCFastQC Report
Tue 7 Mar 2017
1NF_9A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_9A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3380498
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG135650.080.381438
CACCGGC134000.077.9734349
CTTCACC152500.074.67026
CGCTGCC950.072.450451
CCTTCAC166600.071.47795
CGCAGCC307.6063145E-570.592751
CGGGCCC10100.069.710187
GTGTGGC53950.069.167497
GGCTGCC707.2759576E-1268.071581
TTCACCG167200.067.066597
CGGGGCA13150.066.0347194
GGGCCCG10800.065.626548
CGTAGTG25350.064.435864
GCTGCCA1800.062.587952
TGTGGCA63050.060.4501348
GCCTTCA47750.058.783324
GCTGCCG408.097391E-658.676212
ACCTTCA70050.058.1618084
GGCCCGA12050.058.0397539
ACGGGCC11750.057.124776
Produced by FastQC (version 0.11.5)