##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_9A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3380498
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.81617767559691	12.0	12.0	12.0	2.0	32.0
2	14.648606802903004	12.0	12.0	12.0	12.0	22.0
3	13.717862279462967	12.0	12.0	12.0	12.0	22.0
4	13.76863586371002	12.0	12.0	12.0	12.0	22.0
5	22.426837998425086	22.0	22.0	22.0	22.0	27.0
6	26.804464904283332	27.0	22.0	32.0	22.0	32.0
7	34.93981596794318	37.0	37.0	37.0	27.0	41.0
8	37.54358913982496	41.0	37.0	41.0	27.0	41.0
9	38.506381012501706	41.0	37.0	41.0	32.0	41.0
10-11	38.864549394793315	41.0	41.0	41.0	37.0	41.0
12-13	39.04654136757365	41.0	41.0	41.0	37.0	41.0
14-15	38.998514272157536	41.0	41.0	41.0	37.0	41.0
16-17	38.902795239044664	41.0	41.0	41.0	37.0	41.0
18-19	38.85123153452538	41.0	41.0	41.0	37.0	41.0
20-21	38.85873634594666	41.0	41.0	41.0	37.0	41.0
22-23	38.84269477455689	41.0	41.0	41.0	34.5	41.0
24-25	38.866374125942386	41.0	41.0	41.0	37.0	41.0
26-27	38.830325886895956	41.0	41.0	41.0	37.0	41.0
28-29	38.78144551483243	41.0	41.0	41.0	34.5	41.0
30-31	38.66190972454354	41.0	41.0	41.0	32.0	41.0
32-33	38.70083712518097	41.0	41.0	41.0	32.0	41.0
34-35	38.68221915824236	41.0	41.0	41.0	32.0	41.0
36-37	38.603825678938435	41.0	41.0	41.0	32.0	41.0
38-39	38.50233900448987	41.0	41.0	41.0	32.0	41.0
40-41	38.54968705794235	41.0	41.0	41.0	32.0	41.0
42-43	38.2920072723013	41.0	41.0	41.0	32.0	41.0
44-45	38.40205999234432	41.0	41.0	41.0	32.0	41.0
46-47	38.40148152727794	41.0	41.0	41.0	32.0	41.0
48-49	38.362509310758355	41.0	41.0	41.0	32.0	41.0
50-51	38.23067991757428	41.0	41.0	41.0	32.0	41.0
52-53	38.20986833892521	41.0	41.0	41.0	32.0	41.0
54-55	38.07530281041433	41.0	37.0	41.0	32.0	41.0
56-57	37.86820536500835	41.0	37.0	41.0	27.0	41.0
58-59	37.86188736097462	41.0	37.0	41.0	29.5	41.0
60-61	37.862566550845465	41.0	37.0	41.0	29.5	41.0
62-63	37.863196339710896	41.0	37.0	41.0	29.5	41.0
64-65	37.85422769071303	41.0	37.0	41.0	29.5	41.0
66-67	37.71512555250735	41.0	37.0	41.0	27.0	41.0
68-69	37.713524457047455	41.0	37.0	41.0	27.0	41.0
70-71	37.70723100560923	41.0	37.0	41.0	27.0	41.0
72-73	37.541563698602985	41.0	37.0	41.0	27.0	41.0
74-75	37.48951071706003	41.0	37.0	41.0	27.0	41.0
76-77	36.40716293871495	39.0	34.5	41.0	24.5	41.0
78-79	36.93004447865374	41.0	37.0	41.0	27.0	41.0
80-81	37.58262643551335	41.0	37.0	41.0	27.0	41.0
82-83	37.76493226737599	41.0	37.0	41.0	27.0	41.0
84-85	37.69062901383169	41.0	37.0	41.0	27.0	41.0
86-87	37.53855097680874	41.0	37.0	41.0	27.0	41.0
88-89	37.43421235569434	41.0	37.0	41.0	27.0	41.0
90-91	37.31463855325458	41.0	37.0	41.0	27.0	41.0
92-93	37.18240330270865	41.0	37.0	41.0	27.0	41.0
94-95	37.02938117401637	41.0	37.0	41.0	27.0	41.0
96-97	36.95269587498647	41.0	37.0	41.0	27.0	41.0
98-99	36.82866562855532	41.0	37.0	41.0	27.0	41.0
100	36.720273462667336	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3825045691607887
1101	2	-0.6148433308586316
1101	3	0.07142747646379988
1101	4	-0.02634134442228131
1101	5	-1.0774443464016024
1101	6	-0.7032578289251035
1101	7	-0.13253330056607382
1101	8	-0.2800733478025279
1101	9	-0.3087627124172556
1101	10-11	-0.08425140627265648
1101	12-13	-0.07610252119371097
1101	14-15	0.035323039590814176
1101	16-17	-0.16691609113378547
1101	18-19	0.0026513245321950762
1101	20-21	-0.005969696049923812
1101	22-23	-0.011071700051722644
1101	24-25	-0.009456259696726477
1101	26-27	-0.060728714268030615
1101	28-29	0.008730617835965404
1101	30-31	0.04044958935038778
1101	32-33	-0.11776649878727596
1101	34-35	-0.04469792856293253
1101	36-37	-0.03485084166944574
1101	38-39	-0.033447675971736146
1101	40-41	0.06397282104293112
1101	42-43	0.017641421342105446
1101	44-45	-0.03713455569338464
1101	46-47	0.05993443494617168
1101	48-49	0.04626298936356932
1101	50-51	0.04672246616076592
1101	52-53	0.007471985100302447
1101	54-55	0.07462663942824577
1101	56-57	0.1319202519676992
1101	58-59	-0.11939009833128722
1101	60-61	-0.005308028310430757
1101	62-63	-0.08305262984874417
1101	64-65	-0.06646088997260335
1101	66-67	-0.15320326201374002
1101	68-69	-0.06869680897811037
1101	70-71	-0.05691176727889058
1101	72-73	-0.0032570733610484126
1101	74-75	0.16265596925173753
1101	76-77	-0.49955227057161977
1101	78-79	-0.31360655595251785
1101	80-81	-0.16129245095012834
1101	82-83	-0.1265145414562383
1101	84-85	-0.1338458430395093
1101	86-87	-0.22592560794330296
1101	88-89	-0.41227509418189356
1101	90-91	-0.15822598043482827
1101	92-93	0.014518243639734862
1101	94-95	0.025690297361911973
1101	96-97	-0.15444047652365356
1101	98-99	-0.4464062565678688
1101	100	-0.5648331174504833
1102	1	1.7048863465773483
1102	2	0.918726009894975
1102	3	-0.22097861561629273
1102	4	-0.06092238710088438
1102	5	-0.7470698419664608
1102	6	-0.19737068187944118
1102	7	0.06445053136628331
1102	8	-0.1711400479859293
1102	9	-0.25575911312561317
1102	10-11	-0.211490536535905
1102	12-13	-0.2291150257167729
1102	14-15	-0.22268755425285036
1102	16-17	-0.25065468764676524
1102	18-19	-0.03474788572032139
1102	20-21	0.12167760944121397
1102	22-23	-0.10341477916512787
1102	24-25	-0.03963034006249444
1102	26-27	-0.004811300707956434
1102	28-29	-0.09120817278851234
1102	30-31	-0.08180717665281634
1102	32-33	-0.16481199099294486
1102	34-35	-0.004304869683572576
1102	36-37	0.0031655349384323017
1102	38-39	-0.17647279436058483
1102	40-41	-0.09157096733269299
1102	42-43	-0.04621128613005965
1102	44-45	0.09013281204420309
1102	46-47	-0.2291385079171917
1102	48-49	-0.2976361380364736
1102	50-51	-0.0719745744842939
1102	52-53	0.027840447505006694
1102	54-55	-0.053794463039402274
1102	56-57	0.03181338919055321
1102	58-59	-0.0064914905909532195
1102	60-61	-0.05079313233818539
1102	62-63	-0.20233933208373145
1102	64-65	-0.2457844643746867
1102	66-67	-0.15397480450126722
1102	68-69	-0.2169829464636095
1102	70-71	-0.28477381284437087
1102	72-73	-0.1516630932974934
1102	74-75	-0.22047261890836012
1102	76-77	-0.006567112999753988
1102	78-79	-0.2529224763645459
1102	80-81	-0.3771165963015477
1102	82-83	-0.3907347306304061
1102	84-85	-0.25869870945314233
1102	86-87	-0.30164301451446107
1102	88-89	-0.30419489299130476
1102	90-91	-0.305847607296613
1102	92-93	-0.3367978706583443
1102	94-95	-0.15889717061025266
1102	96-97	-0.3660797267257081
1102	98-99	-0.3009149526954502
1102	100	-0.6519281971410322
1103	1	1.5198998240883803
1103	2	0.7166211903145925
1103	3	0.20959706914480591
1103	4	0.8580217136570951
1103	5	2.87242132911274
1103	6	-0.10580459112424734
1103	7	-0.5793964525483446
1103	8	-0.2901742948638315
1103	9	-0.16529641835729336
1103	10-11	-0.22745419435086234
1103	12-13	-0.42602586753475435
1103	14-15	-0.425532684527667
1103	16-17	-0.30101486311500736
1103	18-19	-0.2639954636130426
1103	20-21	-0.1872667811305817
1103	22-23	-0.20222142403498822
1103	24-25	-0.2355417916581004
1103	26-27	-0.18613121098720598
1103	28-29	-0.23756391597514437
1103	30-31	-0.0875930523895363
1103	32-33	-0.2265853967890834
1103	34-35	-0.2887554352762649
1103	36-37	-0.40911185474592315
1103	38-39	-0.301070765336668
1103	40-41	-0.20146865838135142
1103	42-43	-0.06411552790583386
1103	44-45	-0.33847202191000747
1103	46-47	-0.5755671018831308
1103	48-49	-0.35856616225696314
1103	50-51	-0.2780784741395337
1103	52-53	-0.22930778688612463
1103	54-55	-0.20269098525024276
1103	56-57	-0.3615214539792717
1103	58-59	-0.25442160058697993
1103	60-61	-0.2682652126334375
1103	62-63	-0.32432500546496357
1103	64-65	-0.42575037151880935
1103	66-67	-0.46555568277268833
1103	68-69	-0.39845015928908367
1103	70-71	-0.43275537647871687
1103	72-73	-0.25107098497444014
1103	74-75	-0.32449643833192
1103	76-77	-0.4838129889374727
1103	78-79	-0.4851076328020696
1103	80-81	-0.3104448111489404
1103	82-83	-0.3656739573744261
1103	84-85	-0.4746506111109028
1103	86-87	-0.23099861816976386
1103	88-89	-0.22413557932183892
1103	90-91	-0.6705670172730791
1103	92-93	-0.2776025807872671
1103	94-95	-0.30794508548165567
1103	96-97	-0.19012230691941312
1103	98-99	-0.3467262198619494
1103	100	-0.3865226646420368
1104	1	1.4351087917262788
1104	2	-0.5192385727882698
1104	3	0.05723406873668502
1104	4	0.03319072658802469
1104	5	-1.0860562537148688
1104	6	-0.32712179158248844
1104	7	-0.0533518531969861
1104	8	-0.20693349674368733
1104	9	0.09180560828329476
1104	10-11	0.04352747348261232
1104	12-13	0.13884548201369995
1104	14-15	0.05111756755142238
1104	16-17	0.17380880152642675
1104	18-19	0.21824412505409896
1104	20-21	0.21060049919862678
1104	22-23	0.12608256075207436
1104	24-25	-0.013510756448575023
1104	26-27	0.10715228789590725
1104	28-29	0.1887694911067399
1104	30-31	0.1116378538706897
1104	32-33	0.08108635200353831
1104	34-35	0.1686570459851353
1104	36-37	0.0711843488112649
1104	38-39	0.04183694795830917
1104	40-41	0.17260580016385774
1104	42-43	0.09170421757660563
1104	44-45	0.10345868031254213
1104	46-47	-0.05968774473285521
1104	48-49	-0.1635235447960781
1104	50-51	0.10764027543449117
1104	52-53	0.1439473403569309
1104	54-55	0.16899136376124346
1104	56-57	0.10633559359227007
1104	58-59	0.05858014153331226
1104	60-61	0.0514278419187022
1104	62-63	-0.06330469362158908
1104	64-65	-0.1759205582517822
1104	66-67	-0.4574406620561078
1104	68-69	-0.313740972677401
1104	70-71	-0.1454928704189271
1104	72-73	0.022671476915441247
1104	74-75	-0.1824257303609329
1104	76-77	-0.1604018071885278
1104	78-79	-0.02541249977564064
1104	80-81	-0.15035854281534
1104	82-83	-0.17104994743130675
1104	84-85	-0.06460112967509701
1104	86-87	-0.2523690970075947
1104	88-89	-0.25914121065898144
1104	90-91	-0.071785604378924
1104	92-93	-0.09936403182329911
1104	94-95	-0.09942780309609844
1104	96-97	-0.010381349593266975
1104	98-99	-0.020774790683688593
1104	100	0.0930780539228735
1105	1	1.6909339022784717
1105	2	0.3145446227424262
1105	3	-0.03935825024505846
1105	4	-0.23309214452818594
1105	5	-1.1645913609458631
1105	6	0.5986733924314365
1105	7	0.2920725178018557
1105	8	0.17945342375767837
1105	9	0.14245547582614648
1105	10-11	0.053024777324637284
1105	12-13	-0.010175456289026386
1105	14-15	0.10525246188610282
1105	16-17	0.12651899698587243
1105	18-19	0.1321304497718927
1105	20-21	0.14261232463706364
1105	22-23	0.13779303026079504
1105	24-25	0.12551626141510042
1105	26-27	0.08858539362911699
1105	28-29	0.004596931189453812
1105	30-31	0.08260002144635337
1105	32-33	0.00770982594328018
1105	34-35	0.009988880795290811
1105	36-37	-0.11023627171044126
1105	38-39	0.013054180141025995
1105	40-41	0.10406042557736583
1105	42-43	0.1943270165555333
1105	44-45	0.04982978323468501
1105	46-47	-0.18037395339847961
1105	48-49	-0.12732637047415807
1105	50-51	-0.08520699379960206
1105	52-53	0.021263218664756778
1105	54-55	-0.10276489900176244
1105	56-57	0.013476163002792418
1105	58-59	0.03769292989647255
1105	60-61	-0.07607062423173971
1105	62-63	-0.27806965113948223
1105	64-65	-0.13244616957486244
1105	66-67	0.008450644685439102
1105	68-69	-0.05036571523970679
1105	70-71	-0.04351850954896719
1105	72-73	-0.11086104281688591
1105	74-75	-0.1709467062979826
1105	76-77	-0.1769287429714339
1105	78-79	-0.22424256398625175
1105	80-81	-0.21700064853224887
1105	82-83	-0.07924237915964483
1105	84-85	-0.18741937246756635
1105	86-87	-0.17943826396257379
1105	88-89	-0.19756533128354192
1105	90-91	-0.28995116275040544
1105	92-93	-0.03862900575734329
1105	94-95	-0.08728537653829704
1105	96-97	-0.12774552043115506
1105	98-99	-0.36052883201710983
1105	100	-0.23102184385027158
1106	1	1.629737879109996
1106	2	-0.7599777303897657
1106	3	-0.09419763453680297
1106	4	-0.31479861362982575
1106	5	-1.1078823769844917
1106	6	-0.6398782601402502
1106	7	-0.27462972457061596
1106	8	-0.07342829665344652
1106	9	-0.05136785218921602
1106	10-11	-0.19997342080041847
1106	12-13	-0.1809955388117217
1106	14-15	-0.1545045320711722
1106	16-17	-0.13238757729866535
1106	18-19	-0.029801589011654528
1106	20-21	-0.05519385732936399
1106	22-23	-0.0241452956720849
1106	24-25	-0.2297286329784427
1106	26-27	-0.21561523835072194
1106	28-29	-0.22705231382317947
1106	30-31	-0.18917425180330838
1106	32-33	-0.13613132669193817
1106	34-35	-0.16465456062700667
1106	36-37	-0.3168751263815537
1106	38-39	-0.18921587785075644
1106	40-41	-0.2840952689474747
1106	42-43	0.03323833818933508
1106	44-45	-0.0707827792397211
1106	46-47	-0.30775634179165223
1106	48-49	-0.3499362389602254
1106	50-51	-0.3068392657150625
1106	52-53	-0.3795596478517851
1106	54-55	-0.1651734595842882
1106	56-57	-0.1717677430949962
1106	58-59	-0.3719214671019344
1106	60-61	-0.275965573687472
1106	62-63	-0.2880438275008643
1106	64-65	-0.6084092946719224
1106	66-67	-0.4036595299958279
1106	68-69	-0.4742425656470246
1106	70-71	-0.4310146048983299
1106	72-73	-0.298489763926888
1106	74-75	-0.5326691293168011
1106	76-77	-0.5089301565873257
1106	78-79	-0.42646419989809914
1106	80-81	-0.4890545344758621
1106	82-83	-0.4570415945234103
1106	84-85	-0.6187740700490636
1106	86-87	-0.32167961284509516
1106	88-89	-0.43147375862263004
1106	90-91	-0.500834394724599
1106	92-93	-0.4442656226444939
1106	94-95	-0.6090138749287846
1106	96-97	-0.40337381622101987
1106	98-99	-0.5640600701046168
1106	100	-0.5731374056258147
1107	1	1.262716939795423
1107	2	-0.5209476880812147
1107	3	-0.08318315573065327
1107	4	-0.27446653108498076
1107	5	-1.2565710952440838
1107	6	-0.3177459595768539
1107	7	-0.5195890574554412
1107	8	-0.43294023280927263
1107	9	-0.40005227573522006
1107	10-11	-0.25012926718728323
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2224	4	-0.3617948999982854
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2224	9	0.25513588936436804
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2224	24-25	0.3684409026780102
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2224	28-29	0.27341974319362805
2224	30-31	0.42724670089675953
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2224	34-35	0.4904205322137827
2224	36-37	0.3713565183663121
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2224	40-41	0.379402682272719
2224	42-43	0.5440022852491353
2224	44-45	0.6089553294607839
2224	46-47	0.5221988607827583
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2224	50-51	0.4809990272709186
2224	52-53	0.3977372018438672
2224	54-55	0.508053578026697
2224	56-57	0.49265730716468425
2224	58-59	0.5632880529181747
2224	60-61	0.47077191025734777
2224	62-63	0.514148140025803
2224	64-65	0.5542719368297142
2224	66-67	0.4248620502521092
2224	68-69	0.4848887272831135
2224	70-71	0.46725361772411844
2224	72-73	0.5575097230056016
2224	74-75	0.56970703642844
2224	76-77	0.5730411046513026
2224	78-79	0.5077462345688701
2224	80-81	0.45286456489868243
2224	82-83	0.4943731804249367
2224	84-85	0.39676192844314073
2224	86-87	0.4504451149150057
2224	88-89	0.5965774224797329
2224	90-91	0.6428162202583678
2224	92-93	0.46997051755207053
2224	94-95	0.6343472233847223
2224	96-97	0.6323188423761721
2224	98-99	0.4804350121257954
2224	100	0.7875063021420985
2225	1	1.5305996488911848
2225	2	1.7603172302130368
2225	3	0.9214081457104744
2225	4	1.0866991652257223
2225	5	3.2587899787289807
2225	6	0.11526428672519629
2225	7	0.4027610149791059
2225	8	0.44989097441052195
2225	9	0.3077603661858319
2225	10-11	0.3059562573093615
2225	12-13	0.31873922860071247
2225	14-15	0.4144991027684597
2225	16-17	0.39676911706201423
2225	18-19	0.3239700293662011
2225	20-21	0.2734356337227908
2225	22-23	0.4322229552293706
2225	24-25	0.3424525212083438
2225	26-27	0.31212693712320316
2225	28-29	0.36297773049427207
2225	30-31	0.31690577968633704
2225	32-33	0.3072233394686421
2225	34-35	0.3226613361073518
2225	36-37	0.2862457111691441
2225	38-39	0.35773363987622275
2225	40-41	0.33365740160970603
2225	42-43	0.5002796472587718
2225	44-45	0.42649169508356977
2225	46-47	0.3490644848641864
2225	48-49	0.2646423718052944
2225	50-51	0.31327381013069555
2225	52-53	0.38305032208193524
2225	54-55	0.4874192411270357
2225	56-57	0.49862524922940565
2225	58-59	0.5051007803658862
2225	60-61	0.47605425758286657
2225	62-63	0.386919347452924
2225	64-65	0.40477781697924087
2225	66-67	0.46468653669824533
2225	68-69	0.531523964218934
2225	70-71	0.5164499582707478
2225	72-73	0.4573051013459448
2225	74-75	0.5062250605629401
2225	76-77	0.6729547102845856
2225	78-79	0.5903799913874224
2225	80-81	0.403189592407017
2225	82-83	0.35341704808320173
2225	84-85	0.18061245740948095
2225	86-87	0.34958969037626986
2225	88-89	0.4218463603070006
2225	90-91	0.46133602495572035
2225	92-93	0.3796627168918718
2225	94-95	0.5603124211195407
2225	96-97	0.5981750212804755
2225	98-99	0.48452598210984377
2225	100	0.6258269023049365
2226	1	1.318952882487233
2226	2	1.4836160723795686
2226	3	0.8463102175401982
2226	4	0.9622192857284997
2226	5	3.1268655263139458
2226	6	-0.5319684886666494
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2226	8	0.19822999727878
2226	9	0.23946857694508594
2226	10-11	0.025202125186297053
2226	12-13	0.14845924842231994
2226	14-15	0.06355770760010415
2226	16-17	0.06902945523538762
2226	18-19	0.08671134299329708
2226	20-21	0.17766578327628935
2226	22-23	0.12363434662218964
2226	24-25	0.13184157134441676
2226	26-27	0.07201545370151763
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2226	30-31	0.2181803862694096
2226	32-33	0.17215768040026092
2226	34-35	0.11169052971408888
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2226	50-51	0.007920141298335182
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2226	64-65	0.27192832353138385
2226	66-67	0.19254864151154294
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2226	70-71	0.3078308372383347
2226	72-73	0.3556221985294883
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2226	78-79	0.33973598584995557
2226	80-81	0.3236398325735621
2226	82-83	0.24423647876264454
2226	84-85	0.16361398860229315
2226	86-87	0.37708839908781755
2226	88-89	0.29488206148319307
2226	90-91	0.29163876327381644
2226	92-93	0.18544228149824704
2226	94-95	0.4284549911637541
2226	96-97	0.4024517424303369
2226	98-99	0.4072853016729283
2226	100	0.47173587761183455
2227	1	1.2512887297885058
2227	2	1.7424753920925262
2227	3	0.47973998062093237
2227	4	-0.08010233230517017
2227	5	3.2203085212633624
2227	6	-0.0817152843592801
2227	7	0.22285552385483243
2227	8	0.45880634577083157
2227	9	0.06499269047957057
2227	10-11	0.23724864622401043
2227	12-13	0.3234880597492875
2227	14-15	0.2637986077504664
2227	16-17	0.30603784427497516
2227	18-19	0.12072150296040718
2227	20-21	0.17290663333891843
2227	22-23	0.20526867186656972
2227	24-25	0.21287016184988516
2227	26-27	0.2825441535288675
2227	28-29	0.2805228101793631
2227	30-31	0.3172850900724171
2227	32-33	0.22977690441518206
2227	34-35	0.19043185365009663
2227	36-37	0.16693431996808528
2227	38-39	0.1603130810357456
2227	40-41	0.19525039210170547
2227	42-43	0.4151731983186622
2227	44-45	0.2569947224416751
2227	46-47	0.2127400384022664
2227	48-49	0.31198002516519807
2227	50-51	0.33076859066967046
2227	52-53	0.28383204739268564
2227	54-55	0.34401228546436613
2227	56-57	0.48556563723553836
2227	58-59	0.4729504450944475
2227	60-61	0.39328137791650164
2227	62-63	0.33588893833398004
2227	64-65	0.42237947232587914
2227	66-67	0.3424112596096691
2227	68-69	0.3501115111119546
2227	70-71	0.6061373932416387
2227	72-73	0.45646995494811904
2227	74-75	0.3792894979679673
2227	76-77	0.5028092550310888
2227	78-79	0.5196934287107666
2227	80-81	0.4554434325303518
2227	82-83	0.33757031515089153
2227	84-85	0.2872455050145106
2227	86-87	0.36215022693240684
2227	88-89	0.4320428696289298
2227	90-91	0.44626031353663365
2227	92-93	0.48648150366646803
2227	94-95	0.47622381195076713
2227	96-97	0.5604922150493437
2227	98-99	0.6430440565706448
2227	100	0.4808057835166082
2228	1	1.4662384044042636
2228	2	1.4278957686450156
2228	3	-0.03289914717522713
2228	4	-0.09878985698306764
2228	5	3.076720773213083
2228	6	0.17881158800353703
2228	7	0.3569799016428732
2228	8	0.41196026865726765
2228	9	0.33149370358780317
2228	10-11	0.29209152142591677
2228	12-13	0.2740938701059079
2228	14-15	0.27533721322992477
2228	16-17	0.2697031897180793
2228	18-19	0.2769372700457069
2228	20-21	0.24169731310989562
2228	22-23	0.2854242613907232
2228	24-25	0.2946935215469324
2228	26-27	0.21992530731068172
2228	28-29	0.2718606188694537
2228	30-31	0.22122779431805384
2228	32-33	0.17979702421508392
2228	34-35	0.12278893539365043
2228	36-37	0.24055078578367528
2228	38-39	0.32570867534421666
2228	40-41	0.24604212860707975
2228	42-43	0.3960485492827104
2228	44-45	0.3754003110760493
2228	46-47	0.4058790626065303
2228	48-49	0.3066625416253146
2228	50-51	0.27496469977754856
2228	52-53	0.23233059047327487
2228	54-55	0.23836257447010922
2228	56-57	0.4720869890833299
2228	58-59	0.4735038339622477
2228	60-61	0.3750744958987724
2228	62-63	0.42001914552532327
2228	64-65	0.4533812204997574
2228	66-67	0.4526295295096432
2228	68-69	0.523705748620074
2228	70-71	0.39083250789969526
2228	72-73	0.7094850938168022
2228	74-75	0.4985249002739067
2228	76-77	0.5285385478958844
2228	78-79	0.6252783745490831
2228	80-81	0.5432180144122185
2228	82-83	0.4513767688234225
2228	84-85	0.4619736989747736
2228	86-87	0.6211194999912593
2228	88-89	0.4470064248035044
2228	90-91	0.4881159684514884
2228	92-93	0.41830094294149944
2228	94-95	0.661760021604799
2228	96-97	0.476278206815266
2228	98-99	0.6605791820958657
2228	100	0.5799979608780106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	36.0
13	41.0
14	153.0
15	640.0
16	2240.0
17	5626.0
18	9329.0
19	12010.0
20	14162.0
21	16315.0
22	18664.0
23	21198.0
24	24369.0
25	27334.0
26	30827.0
27	34320.0
28	39173.0
29	44533.0
30	50928.0
31	58797.0
32	68296.0
33	82088.0
34	101093.0
35	130032.0
36	182317.0
37	299198.0
38	728870.0
39	1376279.0
40	1630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.308077692177562	18.675198289493487	19.10549424809485	34.9112297702341
2	18.771806612584392	52.70288100223591	11.582376854948787	16.94293553023091
3	41.16366878489501	15.485292403663603	23.641013838789434	19.710024972651958
4	17.394212332029184	16.69259972938898	22.217850742701224	43.695337195880604
5	16.723571497453925	35.2964563209326	26.602737229840102	21.377234951773378
6	17.542977395638157	28.267551112291738	30.25282073824626	23.936650753823844
7	20.22997794999435	26.405429022587796	36.409812992050284	16.95478003536757
8	20.98007453339715	27.85583662525462	33.905063691799256	17.25902514954897
9	21.02308594769173	28.155141638894627	27.31283970586582	23.50893270754782
10-11	19.51385565085381	30.374370876716984	28.51982163574716	21.591951836682053
12-13	23.979321981554197	26.82418389243242	27.424243410290437	21.772250715722947
14-15	21.711460855767406	27.567802140394697	28.71152416004979	22.009212843788102
16-17	20.756054285492848	30.04811717090204	28.11479255423313	21.08103598937198
18-19	21.488135771711743	29.571249561455147	29.575331800225886	19.365282866607227
20-21	22.740081490951923	28.946016237844248	29.992607598052125	18.32129467315171
22-23	21.799569175902487	30.519275562357972	28.6399814465206	19.041173815218944
24-25	22.258096292321426	28.377653233340176	26.402500459991394	22.961750014347
26-27	20.057666059852718	26.709082507961845	30.031876960140192	23.201374472045245
28-29	19.427798674659	28.227281352976057	30.696717353128104	21.648202619236837
30-31	20.071567787484113	30.084490283277205	28.366135605736076	21.477806323502612
32-33	20.49560449377577	30.365821840450725	28.864578532512077	20.27399513326143
34-35	21.124491125272073	29.277698137966656	30.291158284962748	19.306652451798524
36-37	21.794007865113365	28.213609355781312	28.72723190488502	21.265150874220307
38-39	21.587728550322005	29.29875465339157	28.930003554245282	20.183513242041144
40-41	20.610419995249142	29.868378197768752	30.064506338370446	19.45669546861166
42-43	20.67341341235926	29.963978637441745	29.410361325889056	19.95224662430994
44-45	20.462931792889684	30.17900025380876	28.958366489197747	20.39970146410381
46-47	19.75076203458501	28.449108750854922	28.73598104853119	23.06414816602888
48-49	20.818900055553947	28.012869701446352	30.30609691234842	20.862133330651282
50-51	21.901048307083748	30.612294401594088	28.26017054292001	19.22648674840216
52-53	20.37598845952291	30.511312179046012	28.29681378171749	20.815885579713587
54-55	22.215254468719152	28.682069396162053	28.191722989340818	20.910953145777977
56-57	21.325475381575192	28.864837007065326	29.580200352030957	20.229487259328533
58-59	21.30835970276494	28.258913375769986	28.7856116763858	21.64711524507928
60-61	19.63385195136623	30.349545428392716	29.53572861689746	20.480874003343597
62-63	20.61696658858224	31.161337734778417	27.50288304909142	20.718812627547926
64-65	19.620990361025584	30.46486246316922	28.908729181485132	21.005417994320062
66-67	20.08804619911031	30.200372844474394	29.57478454357908	20.136796412836215
68-69	20.754604794914833	30.192252739093473	29.190551214643524	19.862591251348174
70-71	19.566924754873394	30.880612856448963	29.436565263461183	20.11589712521646
72-73	20.050255909040622	30.73808947675757	28.717529192444424	20.494125421757385
74-75	19.969040064511205	29.62106470703429	30.423002764681417	19.98689246377309
76-77	19.312997670757383	30.579266723423594	28.923342063802433	21.18439354201659
78-79	19.518115378266753	29.601526165671448	28.455156607103447	22.42520184895835
80-81	20.520432812482436	29.69965336427161	28.44511778456048	21.334796038685468
82-83	19.8400649845082	28.68422049059044	30.25953572520972	21.216178799691644
84-85	20.538601117350165	29.508625652196805	28.103832630576914	21.848940599876112
86-87	21.24725410279787	28.88240135033359	29.454787430727663	20.415557116140874
88-89	20.844029489146273	28.265583946507288	30.063529101333593	20.826857463012846
90-91	20.528321566822402	28.774429092991628	29.86468265918217	20.832566681003804
92-93	20.49869575429419	29.113018259439883	29.965910348120307	20.422375638145624
94-95	20.6714809474817	29.295269513545048	29.913817431632854	20.119432107340398
96-97	20.02982992446675	29.05483156623669	30.17388266462515	20.741455844671407
98-99	21.50600296169381	29.262419324016758	29.099987634957923	20.131590079331506
100	21.31836788544173	31.145292794138612	27.902042835108908	19.634296485310745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	9.5
2	19.0
3	32.0
4	40.5
5	44.0
6	55.0
7	59.0
8	70.5
9	86.5
10	95.5
11	106.5
12	129.0
13	156.0
14	191.5
15	244.0
16	304.0
17	397.0
18	582.0
19	956.5
20	1544.0
21	2419.0
22	3763.0
23	5730.0
24	8821.5
25	13183.5
26	18834.0
27	25485.0
28	33125.5
29	42174.0
30	52380.5
31	63151.0
32	75406.5
33	89822.0
34	105595.5
35	122619.5
36	138783.5
37	150185.0
38	156994.0
39	161352.0
40	164216.5
41	164918.0
42	162371.0
43	158399.5
44	155282.5
45	150535.0
46	140225.5
47	127409.5
48	116971.5
49	111001.5
50	106468.0
51	98645.0
52	86930.5
53	73701.5
54	61788.5
55	51299.0
56	41412.0
57	31731.5
58	22701.5
59	15846.5
60	11967.0
61	9669.5
62	7523.5
63	5583.5
64	4194.0
65	3549.5
66	3674.5
67	3441.0
68	2422.5
69	2228.5
70	2880.0
71	2739.0
72	1596.0
73	638.0
74	279.5
75	160.0
76	124.0
77	102.0
78	81.5
79	66.0
80	54.0
81	39.0
82	29.0
83	29.5
84	29.0
85	24.0
86	23.5
87	26.0
88	22.0
89	23.0
90	19.5
91	13.0
92	14.5
93	11.5
94	16.5
95	21.0
96	19.0
97	25.5
98	43.0
99	114.0
100	132.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.344216148034993
2	0.006389591119414949
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.8102368349278714E-4
30-31	0.010235178367210985
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.909782523166706E-4
40-41	0.0016269792202213995
42-43	1.1832576147064723E-4
44-45	0.0
46-47	0.0021890265872069738
48-49	0.0
50-51	0.0
52-53	0.0014346998578315976
54-55	0.019006075436222708
56-57	0.011758622546145568
58-59	0.014184300656293836
60-61	2.2186080275746355E-4
62-63	0.011714250385594075
64-65	1.0353504128681632E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.1832576147064723E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3380498.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.98237154239274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05177924917813	61.22228774255909
2	9.090946874412937	13.087758311722922
3	2.604775011523785	5.624936479915363
4	1.1485085511246158	3.306894769866691
5	0.6077563805378994	2.1873872795569453
6	0.35406096874002824	1.5291688920302526
7	0.24783668313559998	1.2487910545110714
8	0.17575224689717214	1.012085082845007
9	0.12130995556384905	0.7858960463869323
>10	0.5572325108337017	6.787742511571164
>50	0.027457642346867643	1.3475388483860407
>100	0.011789035531151166	1.458865963661542
>500	7.112175251208736E-4	0.3252396115161451
>1k	8.36726490038248E-5	0.07540740547078977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.9581440367661807E-5	0.0
3	0.0	0.0	0.0	2.9581440367661807E-5	0.0
4	0.0	0.0	0.0	2.9581440367661807E-5	0.0
5	0.0	0.0	0.0	2.9581440367661807E-5	0.0
6	0.0	0.0	0.0	2.9581440367661807E-5	0.0
7	0.0	0.0	0.0	2.9581440367661807E-5	0.0
8	0.0	0.0	0.0	2.9581440367661807E-5	0.0
9	0.0	0.0	0.0	2.9581440367661807E-5	0.0
10-11	0.0	0.0	0.0	2.9581440367661807E-5	0.0
12-13	0.0	0.0	0.0	2.9581440367661807E-5	0.0
14-15	0.0	0.0	0.0	2.9581440367661807E-5	0.0
16-17	0.0	0.0	0.0	2.9581440367661807E-5	0.0
18-19	0.0	0.0	0.0	2.9581440367661807E-5	0.0
20-21	0.0	0.0	0.0	2.9581440367661807E-5	0.0
22-23	0.0	0.0	1.4790720183830903E-5	2.9581440367661807E-5	0.0
24-25	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
26-27	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
28-29	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
30-31	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
32-33	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
34-35	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
36-37	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
38-39	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
40-41	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	0.0
42-43	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	2.9581440367661807E-5
44-45	0.0	0.0	2.9581440367661807E-5	2.9581440367661807E-5	2.9581440367661807E-5
46-47	2.9581440367661807E-5	0.0	2.9581440367661807E-5	2.9581440367661807E-5	2.9581440367661807E-5
48-49	8.874432110298542E-5	0.0	2.9581440367661807E-5	2.9581440367661807E-5	2.9581440367661807E-5
50-51	1.0353504128681632E-4	0.0	2.9581440367661807E-5	5.9162880735323614E-5	2.9581440367661807E-5
52-53	1.4790720183830902E-4	0.0	2.9581440367661807E-5	5.9162880735323614E-5	2.9581440367661807E-5
54-55	1.9227936238980174E-4	0.0	2.9581440367661807E-5	5.9162880735323614E-5	2.9581440367661807E-5
56-57	2.3665152294129446E-4	0.0	2.2186080275746355E-4	5.9162880735323614E-5	2.9581440367661807E-5
58-59	2.8102368349278714E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
60-61	3.993494449634344E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
62-63	5.176752064340816E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
64-65	5.620473669855743E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
66-67	6.360009679047289E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
68-69	6.803731284562215E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
70-71	7.099545688238833E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
72-73	7.395360091915452E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
74-75	7.543267293753761E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
76-77	9.613968119490087E-4	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
78-79	0.001212839055074134	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
80-81	0.0014051184174639357	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
82-83	0.0016269792202213992	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
84-85	0.0017748864220597084	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
86-87	0.001834049302795032	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
88	0.001834049302795032	0.0	4.141401651472653E-4	5.9162880735323614E-5	2.9581440367661807E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	13565	0.0	80.38143	8
CACCGGC	13400	0.0	77.973434	9
CTTCACC	15250	0.0	74.6702	6
CGCTGCC	95	0.0	72.45045	1
CCTTCAC	16660	0.0	71.4779	5
CGCAGCC	30	7.6063145E-5	70.59275	1
CGGGCCC	1010	0.0	69.71018	7
GTGTGGC	5395	0.0	69.16749	7
GGCTGCC	70	7.2759576E-12	68.07158	1
TTCACCG	16720	0.0	67.06659	7
CGGGGCA	1315	0.0	66.03471	94
GGGCCCG	1080	0.0	65.62654	8
CGTAGTG	2535	0.0	64.43586	4
GCTGCCA	180	0.0	62.58795	2
TGTGGCA	6305	0.0	60.450134	8
GCCTTCA	4775	0.0	58.78332	4
GCTGCCG	40	8.097391E-6	58.67621	2
ACCTTCA	7005	0.0	58.161808	4
GGCCCGA	1205	0.0	58.039753	9
ACGGGCC	1175	0.0	57.12477	6
>>END_MODULE