FastQCFastQC Report
Tue 7 Mar 2017
1SN_10A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_10A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2558667
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA145080.5670139959596149No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG74260.290229248276544No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA58460.22847834438791764No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA57900.22628970475642196No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT57450.22453097648111303No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG46300.18095359810401276No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG45630.1783350471163305No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG43490.16997131709597224No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC41860.1636008124542975No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT36150.14128450478315466No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG35350.13815787673816093No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35090.13714172262353796No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34660.13546116004935382No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA34470.1347185858886678No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG33080.1292860696604912No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC32870.12846532979868033No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT30940.12092233964013292No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC30790.1203360968816966No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA30370.11869461715807489No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA27600.10786866755228405No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA27540.10763417044890952No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA26070.10188899141623353No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC26030.10173266001398384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGC800.087.9995961
GCTGCTT202.5504125E-587.9995963
GCTGCCG202.5504125E-587.9995963
GCTGCAA302.0971129E-887.9995963
TGCTGCC158.9718786E-487.9995962
GCAGCGG302.0971129E-887.9995963
GCAGCCT158.9718786E-487.9995963
GCAGCAC202.5504125E-587.9995963
AGCTGCG700.087.999592
GCTGCCA450.087.999583
CAGCTGC5150.087.1452261
AGCTGCC2150.085.953082
CAGCAGC3200.085.24961
GCTGCCC1400.084.856753
GCAGCAA900.083.110723
GCTGCAT700.081.7139053
AGCAGCC700.081.7139052
AGCTGCT1350.081.48112
CAGCCCT152200.081.2637561
AGCAGCA1300.081.2303852
Produced by FastQC (version 0.11.5)