FastQCFastQC Report
Tue 7 Mar 2017
1SN_10A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_10A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2558667
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG97100.079.327338
GCAGCCT555.820766E-1176.932861
CACCGGC96600.075.850649
CTTCACC107750.074.5360266
CCTTCAC119650.070.732095
GTGTGGC44600.069.8822257
CGTAGTG24750.068.843814
CAGCGTA11100.067.6627961
CGCTGCC652.582965E-1065.097041
TTCACCG122400.064.847867
GCGTAGT17150.064.3186953
TGTGGCA49900.062.9301958
CGGGGCA9900.062.5852894
CGGGCCC6550.062.3464057
ACCTTCA47500.060.279514
ACGCAGC551.2996679E-859.745172
AGGCTGC551.2996679E-859.745172
GGCGCGC3250.059.215316
TGTGTGG50050.057.3958936
AGCGTAG15400.057.0017662
Produced by FastQC (version 0.11.5)