FastQCFastQC Report
Tue 7 Mar 2017
1SN_11A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_11A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2583992
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA140800.5448933278431203No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG81320.31470685667757486No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60640.2346756491506166No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA54930.2125780575172059No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA51960.20108421388301512No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG51270.19841392697810212No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG49480.19148666094941472No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG46790.18107641200127556No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT40650.1573147285285713No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA40550.15692773042640998No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA37940.14682707995999986No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT36920.14287969931795455No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC36350.1406738101356351No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC36220.14017071260282538No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC36070.13959021544958344No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33320.1289477676401475No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA32460.12561958396156025No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA28930.1119585509552661No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA27340.10580528113090133No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG26640.10309629441577219No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC26240.10154830200712694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC550.087.999743
GCAGCTA550.087.999743
CAGCAGC6650.087.999731
GCTGCAT700.087.999733
AGCTGCC2550.087.999732
GCAGCCA700.087.999733
GCAGCAT850.087.999733
GCTGCTA202.5503978E-587.9997253
GCTGCCG158.971833E-487.9997253
GCTGCCC1600.087.9997253
CAGCTGC7150.087.9997251
GCAGCTT302.0971129E-887.9997253
GCAGCGG450.087.9997253
GCAGCCG401.8189894E-1187.9997253
AGCAGCT1350.087.9997252
AGCTGCT2250.086.0441742
AGCTGCA1550.085.161032
AGCAGCA3100.085.161032
AGCAGCC1500.085.066412
GCAGCAA1350.084.740483
Produced by FastQC (version 0.11.5)