FastQCFastQC Report
Tue 7 Mar 2017
1SN_11A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_11A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2583992
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG95200.078.593368
CACCGGC93300.075.967859
CTTCACC106050.073.119616
GTGTGGC40200.070.75897
CGCTGCA601.2551027E-1070.648361
CCTTCAC116700.069.423175
CGTAGTG22600.068.539364
GCAGCCT707.2759576E-1268.1252061
TTCACCG119400.064.5510567
CAGCTGC1000.063.5835271
CGGGGCA11750.063.5175694
CAGCGTA11100.059.6692241
GCCTTCA34200.059.2914664
GCGTAGT14450.058.795663
ACCTTCA45400.058.1051984
TGTGGCA47600.058.0821728
TGTGTGG46050.057.7946976
GCTGCGG657.4578566E-1057.7745252
CGGGCCC6700.057.4478237
AGCAGCA1100.055.4766732
Produced by FastQC (version 0.11.5)