##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_11A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2583992 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.775564707630673 12.0 12.0 12.0 2.0 27.0 2 14.598545196734355 12.0 12.0 12.0 12.0 22.0 3 13.70300836844696 12.0 12.0 12.0 12.0 22.0 4 13.76540794243945 12.0 12.0 12.0 12.0 22.0 5 22.44991238362967 22.0 22.0 22.0 22.0 27.0 6 26.843908572472362 27.0 22.0 32.0 22.0 32.0 7 34.988958557147235 37.0 37.0 37.0 27.0 41.0 8 37.62784482304899 41.0 37.0 41.0 32.0 41.0 9 38.562556695221964 41.0 41.0 41.0 32.0 41.0 10-11 38.918316697574916 41.0 41.0 41.0 37.0 41.0 12-13 39.09894109579287 41.0 41.0 41.0 37.0 41.0 14-15 39.04784747785597 41.0 41.0 41.0 37.0 41.0 16-17 38.96107727887703 41.0 41.0 41.0 37.0 41.0 18-19 38.893562170471114 41.0 41.0 41.0 37.0 41.0 20-21 38.90208967365224 41.0 41.0 41.0 37.0 41.0 22-23 38.89886965594321 41.0 41.0 41.0 37.0 41.0 24-25 38.919282064340756 41.0 41.0 41.0 37.0 41.0 26-27 38.89118677612005 41.0 41.0 41.0 37.0 41.0 28-29 38.84531376258131 41.0 41.0 41.0 37.0 41.0 30-31 38.71121717869096 41.0 41.0 41.0 32.0 41.0 32-33 38.75531870841705 41.0 41.0 41.0 32.0 41.0 34-35 38.725860412880536 41.0 41.0 41.0 32.0 41.0 36-37 38.653273887844854 41.0 41.0 41.0 32.0 41.0 38-39 38.55709828049003 41.0 41.0 41.0 32.0 41.0 40-41 38.60756089802136 41.0 41.0 41.0 32.0 41.0 42-43 38.34107458537024 41.0 41.0 41.0 32.0 41.0 44-45 38.451306544292706 41.0 41.0 41.0 32.0 41.0 46-47 38.46535476890021 41.0 41.0 41.0 32.0 41.0 48-49 38.409677158443216 41.0 41.0 41.0 32.0 41.0 50-51 38.278998154793044 41.0 41.0 41.0 32.0 41.0 52-53 38.273719307180514 41.0 41.0 41.0 32.0 41.0 54-55 38.14258035628593 41.0 39.0 41.0 32.0 41.0 56-57 37.931141234183386 41.0 37.0 41.0 29.5 41.0 58-59 37.93026487698104 41.0 37.0 41.0 29.5 41.0 60-61 37.93694117474048 41.0 37.0 41.0 32.0 41.0 62-63 37.93206054817507 41.0 37.0 41.0 32.0 41.0 64-65 37.90813129452413 41.0 37.0 41.0 29.5 41.0 66-67 37.78970716627606 41.0 37.0 41.0 27.0 41.0 68-69 37.79146568565228 41.0 37.0 41.0 27.0 41.0 70-71 37.784893877380426 41.0 37.0 41.0 27.0 41.0 72-73 37.64778606125715 41.0 37.0 41.0 27.0 41.0 74-75 37.54616868008879 41.0 37.0 41.0 27.0 41.0 76-77 36.515497725999154 39.0 34.5 41.0 24.5 41.0 78-79 37.03604268124669 41.0 37.0 41.0 27.0 41.0 80-81 37.677143350289015 41.0 37.0 41.0 27.0 41.0 82-83 37.867351176009834 41.0 37.0 41.0 32.0 41.0 84-85 37.783366782869294 41.0 37.0 41.0 27.0 41.0 86-87 37.64552541184338 41.0 37.0 41.0 27.0 41.0 88-89 37.5543351140406 41.0 37.0 41.0 27.0 41.0 90-91 37.41434300106192 41.0 37.0 41.0 27.0 41.0 92-93 37.30649069346964 41.0 37.0 41.0 27.0 41.0 94-95 37.14775084442986 41.0 37.0 41.0 27.0 41.0 96-97 37.079476639246565 41.0 37.0 41.0 27.0 41.0 98-99 36.96475027786464 41.0 37.0 41.0 27.0 41.0 100 36.83394530633222 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4558346285289296 1101 2 -0.5890315786893296 1101 3 -0.13969790210090594 1101 4 0.12293903585503685 1101 5 -1.105171856610717 1101 6 -0.8309008773577595 1101 7 -0.6313358272976188 1101 8 -0.44057541867631755 1101 9 -0.2638623932279174 1101 10-11 -0.16937943569722336 1101 12-13 -0.26749253192480893 1101 14-15 -0.20850587682357968 1101 16-17 -0.20002329814116848 1101 18-19 -0.27719030872871286 1101 20-21 -0.20679400979518192 1101 22-23 -0.13323181522873995 1101 24-25 -0.20116590709685056 1101 26-27 -0.25284388271855107 1101 28-29 -0.21009430579650967 1101 30-31 -0.1478357323433812 1101 32-33 -0.20076463590044114 1101 34-35 -0.17035129873221422 1101 36-37 0.012982637566764765 1101 38-39 -0.1481465374543376 1101 40-41 -0.06244461754243247 1101 42-43 -0.13648972293010786 1101 44-45 -0.33189823305627186 1101 46-47 -0.22822801412305438 1101 48-49 -0.1441839313269213 1101 50-51 -0.1849068380851051 1101 52-53 -0.11511103384408017 1101 54-55 -0.16501833112145903 1101 56-57 0.10464547991444562 1101 58-59 -0.10536725234106115 1101 60-61 -0.22865600499228123 1101 62-63 -0.33168650592774185 1101 64-65 -0.13013430336582843 1101 66-67 -0.31047511254061533 1101 68-69 -0.1867954649582444 1101 70-71 -0.22662099715113726 1101 72-73 -0.2221991096141025 1101 74-75 -0.17844410875638061 1101 76-77 -0.5059265505341202 1101 78-79 -0.16967531164306848 1101 80-81 -0.2453914647116946 1101 82-83 -0.218169484855963 1101 84-85 -0.18624271198552123 1101 86-87 -0.3182048437536764 1101 88-89 -0.3609974430415619 1101 90-91 -0.2999575981538243 1101 92-93 -0.08532620115781953 1101 94-95 -0.13079762574436415 1101 96-97 -0.34769568542798623 1101 98-99 -0.488550847024662 1101 100 -0.5735286291494432 1102 1 1.6795886287659805 1102 2 0.8515229233058506 1102 3 -0.13732739125581972 1102 4 0.015661073704192674 1102 5 -0.8313161219498966 1102 6 -0.09524246797053593 1102 7 0.030666365424913522 1102 8 -0.36609446178010785 1102 9 -0.0851357526368659 1102 10-11 -0.24506169834982927 1102 12-13 -0.14192953559910393 1102 14-15 -0.0719718599929493 1102 16-17 -0.23524760772988174 1102 18-19 -0.150008697966598 1102 20-21 -0.14121111105550455 1102 22-23 0.029765498192297457 1102 24-25 0.032397316402814624 1102 26-27 -0.006174944312327568 1102 28-29 -0.10000012737606312 1102 30-31 -0.04550621158165313 1102 32-33 -0.13005056889399214 1102 34-35 0.04280824652479964 1102 36-37 -0.09574849711776778 1102 38-39 -0.03530281338179719 1102 40-41 -0.13099806268485992 1102 42-43 -0.03804067903614339 1102 44-45 0.03095907206797932 1102 46-47 -0.2737850347860018 1102 48-49 -0.1304275605790295 1102 50-51 -0.16817621701127194 1102 52-53 -0.16179898572271156 1102 54-55 -0.10570617435167406 1102 56-57 -0.03163543549782588 1102 58-59 -0.20944996341530242 1102 60-61 -0.1496451500280287 1102 62-63 -0.2878604520381316 1102 64-65 -0.1372976908258252 1102 66-67 -0.11014348795050921 1102 68-69 -0.27930958925202987 1102 70-71 -0.3473935861440651 1102 72-73 -0.1198720581345114 1102 74-75 -0.38550699643367636 1102 76-77 -0.06796479477575446 1102 78-79 -0.36536715511034146 1102 80-81 -0.24027140821451098 1102 82-83 -0.22145486473032605 1102 84-85 -0.28157799111317416 1102 86-87 -0.23216147328233916 1102 88-89 -0.39334010038422207 1102 90-91 -0.4304171809439197 1102 92-93 -0.3089517987240953 1102 94-95 -0.2616362433414565 1102 96-97 -0.26721437295514505 1102 98-99 -0.29030453119359834 1102 100 -0.21043303829961246 1103 1 1.4564542181315687 1103 2 0.8082340050169314 1103 3 0.3185253145968865 1103 4 0.7864314069745255 1103 5 2.9230033198695438 1103 6 -0.22181904454711088 1103 7 -0.2984341511756057 1103 8 -0.03695772014336285 1103 9 0.0178788003776873 1103 10-11 -0.06676482630295766 1103 12-13 -0.1286554223249894 1103 14-15 -0.02163559715668839 1103 16-17 -0.18347966846194907 1103 18-19 -0.11084195254984763 1103 20-21 0.07424713565274743 1103 22-23 0.03339902682581908 1103 24-25 -0.1234526331971324 1103 26-27 -0.10004897568635585 1103 28-29 -0.05927885540478428 1103 30-31 0.04925454567910492 1103 32-33 -0.1582214408148701 1103 34-35 -0.159127036660486 1103 36-37 -0.015912848532117607 1103 38-39 0.1519955864166036 1103 40-41 -0.06467514011193742 1103 42-43 0.05389019414472784 1103 44-45 -0.2322579473990416 1103 46-47 -0.3564501737011412 1103 48-49 -0.26371128136275246 1103 50-51 0.0821982396131844 1103 52-53 -0.11507932025304513 1103 54-55 0.016139097493592658 1103 56-57 -0.01751909013147923 1103 58-59 -0.1971877886531317 1103 60-61 -0.02243896657184763 1103 62-63 0.026844156416480303 1103 64-65 -0.25829009931823776 1103 66-67 -0.026186847743872477 1103 68-69 -0.04005109999354062 1103 70-71 -0.21842773217821332 1103 72-73 -0.029161417423864577 1103 74-75 -0.08856368074035714 1103 76-77 -0.21905627086723456 1103 78-79 -0.15474731324049884 1103 80-81 0.011188224495121801 1103 82-83 -0.010351497376959173 1103 84-85 -0.20783996112513847 1103 86-87 -0.03139799126885379 1103 88-89 -0.09294477498889364 1103 90-91 -0.43919230221904115 1103 92-93 -0.15436933164162525 1103 94-95 0.0031573527021322434 1103 96-97 0.11067024242947099 1103 98-99 -0.058176861565932825 1103 100 -0.051256291622870265 1104 1 1.5673781307496615 1104 2 -0.5290833296939734 1104 3 -0.13169227919151538 1104 4 0.11560267207107344 1104 5 -0.9941173194491846 1104 6 -0.411706412366712 1104 7 -0.08283823379862554 1104 8 0.24700686998977517 1104 9 0.2777092650734829 1104 10-11 0.13198596619240277 1104 12-13 0.18740327320807637 1104 14-15 0.16841085676900036 1104 16-17 0.19256485999353146 1104 18-19 0.020761588436833733 1104 20-21 0.17083568771479918 1104 22-23 0.18963332367697916 1104 24-25 0.06676523454027716 1104 26-27 0.09092479565231315 1104 28-29 0.19123245086238683 1104 30-31 0.08822555404628929 1104 32-33 0.14217148034260418 1104 34-35 0.012943861344382412 1104 36-37 0.04874675024998254 1104 38-39 0.1850858878387882 1104 40-41 0.11216442751824474 1104 42-43 0.13557298466765388 1104 44-45 0.15870467030119784 1104 46-47 0.05554644498531047 1104 48-49 -0.006376492186085159 1104 50-51 0.08047177379028625 1104 52-53 0.07421670905167588 1104 54-55 0.04850147724954468 1104 56-57 0.20735919823508908 1104 58-59 0.33605964209647965 1104 60-61 0.2199914308107651 1104 62-63 0.16761931471987168 1104 64-65 0.1253923233303027 1104 66-67 -0.10391087933410859 1104 68-69 -0.0667268852488192 1104 70-71 0.22057634574854745 1104 72-73 0.02848176007161385 1104 74-75 0.04142356106270029 1104 76-77 -0.08173208743097149 1104 78-79 0.14445764758479385 1104 80-81 -0.06139476248838349 1104 82-83 0.050443659204475466 1104 84-85 0.15471923142970923 1104 86-87 0.13581950859416736 1104 88-89 0.1858347430173879 1104 90-91 0.11028739033189794 1104 92-93 0.05489151478255394 1104 94-95 -0.12015942628362808 1104 96-97 -0.10974678624125289 1104 98-99 -0.017424988928745222 1104 100 -0.1153572300939203 1105 1 1.692028773885399 1105 2 0.21900857495197457 1105 3 -0.003276920519734716 1105 4 -0.2459125898425789 1105 5 -1.1922141056889402 1105 6 0.5137431026459929 1105 7 0.18633778735672024 1105 8 0.09051246976670058 1105 9 -0.004470790139812664 1105 10-11 0.049289487739990534 1105 12-13 0.030984946071392017 1105 14-15 0.04301467839214013 1105 16-17 -0.017395908707435126 1105 18-19 -0.0642011272032974 1105 20-21 -0.04402614418589934 1105 22-23 -0.08373365023251722 1105 24-25 0.006142867419356435 1105 26-27 1.1765355766613084E-4 1105 28-29 -0.11366173631805765 1105 30-31 0.0017999747986436887 1105 32-33 -0.01324612006342818 1105 34-35 -0.08365974602047999 1105 36-37 -0.11233691798854295 1105 38-39 0.0016503132903977757 1105 40-41 0.10139433994967106 1105 42-43 0.15208179413999545 1105 44-45 1.3046418701634366E-4 1105 46-47 -0.105133684496586 1105 48-49 -0.01814901599287566 1105 50-51 0.008306146820793003 1105 52-53 -0.08697570286215495 1105 54-55 -0.04316279294077674 1105 56-57 0.03151136796106613 1105 58-59 -0.03707077395588243 1105 60-61 -0.1363995821940307 1105 62-63 -0.18788588845130505 1105 64-65 -0.19096164274623106 1105 66-67 -0.11071652390013753 1105 68-69 0.05803611052261459 1105 70-71 -0.20597209770683378 1105 72-73 -0.17057823870514 1105 74-75 -0.05946521191735599 1105 76-77 -0.018798940953146825 1105 78-79 -0.1245843671724387 1105 80-81 -0.23198775647053793 1105 82-83 -0.1055172796874757 1105 84-85 -0.13574927621197475 1105 86-87 -0.21711032143899445 1105 88-89 -0.12107951226224145 1105 90-91 -0.07384281412140581 1105 92-93 -0.0732739708676462 1105 94-95 -0.05289989606390577 1105 96-97 -0.02680242919274889 1105 98-99 -0.11139481498064896 1105 100 -0.28929069681949215 1106 1 1.2805896438798978 1106 2 -0.6483774451429056 1106 3 -0.09785073887594109 1106 4 -0.21483276041867327 1106 5 -1.147949891406249 1106 6 -0.5931994572178461 1106 7 -0.23675731304715697 1106 8 -0.05969529642932514 1106 9 -0.10827057480394586 1106 10-11 -0.07891245780542988 1106 12-13 -0.11089750311545998 1106 14-15 -0.0960776198245128 1106 16-17 -0.11455639034512188 1106 18-19 0.013668364218531792 1106 20-21 -0.2858109869860215 1106 22-23 -0.07703689296493366 1106 24-25 -0.13937678315354418 1106 26-27 -0.10680863857504619 1106 28-29 -0.2148252082092057 1106 30-31 -0.31410930203958287 1106 32-33 -0.21021214119266318 1106 34-35 -0.300591727157439 1106 36-37 -0.4354493204879475 1106 38-39 -0.1230174911771229 1106 40-41 -0.17048740398872297 1106 42-43 -0.0732981232048786 1106 44-45 -0.11154158587622476 1106 46-47 -0.2837242219429328 1106 48-49 -0.2247923624438286 1106 50-51 -0.43065412888434196 1106 52-53 -0.3088834469603938 1106 54-55 -0.4252151599896905 1106 56-57 -0.16106311803034146 1106 58-59 -0.14194657970224256 1106 60-61 -0.2343017971443615 1106 62-63 -0.5585045794161232 1106 64-65 -0.571710616609721 1106 66-67 -0.4901479055758955 1106 68-69 -0.5272045437598862 1106 70-71 -0.5185526958515609 1106 72-73 -0.3180628995511583 1106 74-75 -0.5222079701910971 1106 76-77 -0.463605820416781 1106 78-79 -0.3336676852253859 1106 80-81 -0.3839076778588506 1106 82-83 -0.44555056741474175 1106 84-85 -0.5026016583061903 1106 86-87 -0.6233930508445624 1106 88-89 -0.5986814794352782 1106 90-91 -0.6359941040208383 1106 92-93 -0.5574774397497322 1106 94-95 -0.48252268217144945 1106 96-97 -0.34868459628020787 1106 98-99 -0.7277932521500645 1106 100 -0.8104948850550073 1107 1 1.653877050800304 1107 2 -0.6076572961202711 1107 3 -0.13122361466023946 1107 4 -0.11273415202709813 1107 5 -1.2706534516560808 1107 6 -0.3627435756355766 1107 7 -0.21539619518682684 1107 8 -0.15073707326698127 1107 9 -0.22607334849249838 1107 10-11 -0.1371237097765956 1107 12-13 -0.2380225763642727 1107 14-15 -0.2612936978803617 1107 16-17 -0.2106433075108285 1107 18-19 -0.13051408369739192 1107 20-21 -0.22622546809854782 1107 22-23 -0.04057084468502836 1107 24-25 -0.2579711681255006 1107 26-27 -0.21480307404701193 1107 28-29 -0.10025138531174349 1107 30-31 -0.2936822435691653 1107 32-33 -0.19159908074988152 1107 34-35 -0.4247155760195227 1107 36-37 -0.2621504796058147 1107 38-39 -0.16385140811891574 1107 40-41 -0.4637742392110411 1107 42-43 -0.2938612458198264 1107 44-45 -0.12218927216643038 1107 46-47 -0.3458387425979055 1107 48-49 -0.372690752637304 1107 50-51 -0.24235869436997604 1107 52-53 -0.268247242273425 1107 54-55 -0.30746155233656225 1107 56-57 -0.06800277053319093 1107 58-59 -0.3566952469310749 1107 60-61 -0.15072134731324383 1107 62-63 -0.2930015089209945 1107 64-65 -0.22418878488905847 1107 66-67 -0.2178645910609589 1107 68-69 -0.5782470828616582 1107 70-71 -0.4992060514811172 1107 72-73 -0.270034257477036 1107 74-75 -0.3494483358873026 1107 76-77 -0.4105826332952347 1107 78-79 -0.6771627614756213 1107 80-81 -0.5737733764500845 1107 82-83 -0.4291965719597357 1107 84-85 -0.25020076455151496 1107 86-87 -0.4368218577582965 1107 88-89 -0.32689840074579735 1107 90-91 -0.13043805616970872 1107 92-93 -0.33626639263705016 1107 94-95 -0.24157385391431774 1107 96-97 -0.31861642128342993 1107 98-99 -0.48828319740997017 1107 100 -0.4070530720098091 1108 1 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0.22257841077749418 2223 16-17 0.29713581687154544 2223 18-19 0.33866264966624726 2223 20-21 0.24297356273058313 2223 22-23 0.40860861819594163 2223 24-25 0.2793590179333947 2223 26-27 0.28464938862155265 2223 28-29 0.42392598718183194 2223 30-31 0.3559179878631511 2223 32-33 0.26897755861840267 2223 34-35 0.32564949692299194 2223 36-37 0.3324255658041295 2223 38-39 0.2891220838467916 2223 40-41 0.3624144223355401 2223 42-43 0.46444451202007997 2223 44-45 0.39824126113705205 2223 46-47 0.3611408446490927 2223 48-49 0.3692351140565293 2223 50-51 0.41058394636115025 2223 52-53 0.3108455199884972 2223 54-55 0.4256933371996823 2223 56-57 0.4720155095441214 2223 58-59 0.6237689055682054 2223 60-61 0.5091511919163523 2223 62-63 0.5748679394185672 2223 64-65 0.4837004812840604 2223 66-67 0.573708600850054 2223 68-69 0.6069535510783552 2223 70-71 0.4986688655040723 2223 72-73 0.6158787420414527 2223 74-75 0.5899036151889732 2223 76-77 0.6409205714848767 2223 78-79 0.7626614729860748 2223 80-81 0.5197892875972698 2223 82-83 0.5262512659069358 2223 84-85 0.3686716372411283 2223 86-87 0.5744876666233054 2223 88-89 0.5733759926333946 2223 90-91 0.6289082820962975 2223 92-93 0.7049665226508424 2223 94-95 0.6824156239886037 2223 96-97 0.759694103600836 2223 98-99 0.5657341427138434 2223 100 0.8446241875547926 2224 1 1.1560153672635334 2224 2 -0.9958877132678765 2224 3 0.09611298810730595 2224 4 -0.24391116735946383 2224 5 -1.1035500575422041 2224 6 0.6938133260208801 2224 7 0.36119736875634345 2224 8 0.20132090182624296 2224 9 0.005339651787032551 2224 10-11 0.11261691595431955 2224 12-13 0.22775818011865567 2224 14-15 0.18857880179162123 2224 16-17 0.22976222149668502 2224 18-19 0.029536193836875668 2224 20-21 0.09805721182333826 2224 22-23 0.17914198007950688 2224 24-25 0.19928351496820085 2224 26-27 0.09964943517084635 2224 28-29 0.06942141138560487 2224 30-31 0.2077348070563616 2224 32-33 0.17916208004504597 2224 34-35 0.22403423620035312 2224 36-37 0.1355322661104168 2224 38-39 0.17972052165227126 2224 40-41 0.21611788467250648 2224 42-43 0.25379449758980144 2224 44-45 0.2552986005652329 2224 46-47 0.2776583983902654 2224 48-49 0.19991436144744767 2224 50-51 0.18747314971642481 2224 52-53 0.1873101937244499 2224 54-55 0.307151354385681 2224 56-57 0.19003707118005053 2224 58-59 0.356768635582263 2224 60-61 0.19250516210618684 2224 62-63 0.2406988909793668 2224 64-65 0.22928481465152828 2224 66-67 0.24931676745931242 2224 68-69 0.18704069136863666 2224 70-71 0.19290699310501225 2224 72-73 0.2945191300764236 2224 74-75 0.32216786625728844 2224 76-77 0.29015669746591044 2224 78-79 0.22984747468047573 2224 80-81 0.24441619495485156 2224 82-83 0.3552538074987268 2224 84-85 0.263730429007488 2224 86-87 0.3585152051893985 2224 88-89 0.20828284748336756 2224 90-91 0.55117327795341 2224 92-93 0.22954688070806384 2224 94-95 0.43516156721707944 2224 96-97 0.29135693770843574 2224 98-99 0.4461787816309126 2224 100 0.4649289923735722 2225 1 1.55582498968133 2225 2 1.5925552912837428 2225 3 0.8832797577559983 2225 4 1.0578029730931746 2225 5 3.197798543108348 2225 6 0.018138422647709262 2225 7 0.22542920634533914 2225 8 0.45124176338686084 2225 9 0.16647720422900392 2225 10-11 0.21277268786491277 2225 12-13 0.2608437071546845 2225 14-15 0.21105653076890007 2225 16-17 0.38573341621806634 2225 18-19 0.17552184315246677 2225 20-21 0.1678894160654565 2225 22-23 0.15398245071664007 2225 24-25 0.11804285061352715 2225 26-27 0.16577521426235364 2225 28-29 0.15512416629395887 2225 30-31 0.2581119439333861 2225 32-33 0.08382994909644026 2225 34-35 0.12789578382536604 2225 36-37 0.26436519215448584 2225 38-39 0.23521382410955027 2225 40-41 0.19907732830979086 2225 42-43 0.4674706033476994 2225 44-45 0.3109528704226747 2225 46-47 0.2793317171391081 2225 48-49 0.2731497041363653 2225 50-51 0.37013802765024906 2225 52-53 0.19746552030736098 2225 54-55 0.2884063063366824 2225 56-57 0.2667136834981889 2225 58-59 0.417968905005516 2225 60-61 0.38069841205190613 2225 62-63 0.36084440179804744 2225 64-65 0.3729645015861749 2225 66-67 0.5084312333722636 2225 68-69 0.5268986318394582 2225 70-71 0.37881756759627194 2225 72-73 0.40958141100767875 2225 74-75 0.37284188825275777 2225 76-77 0.4944832414076856 2225 78-79 0.33685683955042833 2225 80-81 0.3515496949149579 2225 82-83 0.37312731217188855 2225 84-85 0.22155353743178097 2225 86-87 0.16383956145896406 2225 88-89 0.16783623948861504 2225 90-91 0.39701929848672535 2225 92-93 0.4286704201724447 2225 94-95 0.5061974550613044 2225 96-97 0.43488122383889305 2225 98-99 0.5961360116185332 2225 100 0.5551037649317436 2226 1 1.5217127128159014 2226 2 1.3070094224526372 2226 3 0.8621037995629308 2226 4 1.0206235155685484 2226 5 3.0743431111614896 2226 6 -0.49726508650033097 2226 7 0.17628360703497492 2226 8 0.09808101585230844 2226 9 0.0978618717483144 2226 10-11 -0.0812917063800711 2226 12-13 0.08229420716531166 2226 14-15 -0.008777578198191804 2226 16-17 0.008773622236326162 2226 18-19 -0.06591172436603188 2226 20-21 -0.1237610073314741 2226 22-23 -0.10777339831788879 2226 24-25 0.04413423379164527 2226 26-27 -0.042626829747462125 2226 28-29 0.06993684354775098 2226 30-31 -0.05054393701411897 2226 32-33 -0.07304731497088568 2226 34-35 -0.030242954666363175 2226 36-37 0.04575428837425477 2226 38-39 0.06229187661719493 2226 40-41 -0.0052023571941788305 2226 42-43 0.10749605098024517 2226 44-45 0.007277161872913496 2226 46-47 0.07951552231239845 2226 48-49 0.14639221055892193 2226 50-51 0.11095956945107588 2226 52-53 0.09664845798190669 2226 54-55 0.11464882949614719 2226 56-57 0.1420991731949215 2226 58-59 0.12785178985534884 2226 60-61 0.09471381038954263 2226 62-63 -0.004285026267723424 2226 64-65 -0.031154133426291253 2226 66-67 -0.07646977529952892 2226 68-69 0.10298455201340317 2226 70-71 0.14706470675920968 2226 72-73 0.19857743495613533 2226 74-75 0.04653599048371859 2226 76-77 0.3418371257917201 2226 78-79 0.11076481698454899 2226 80-81 0.034460289513596365 2226 82-83 -0.1256052896642501 2226 84-85 0.02154815905245755 2226 86-87 0.15250932840401532 2226 88-89 -0.02938169037317806 2226 90-91 0.163388236127048 2226 92-93 0.036656241560976355 2226 94-95 0.13410960769745373 2226 96-97 0.02332791968044745 2226 98-99 0.12103442888938076 2226 100 0.1822690562565441 2227 1 1.1409455177573005 2227 2 1.5792270275277343 2227 3 0.47918532412940174 2227 4 -0.13484134018720617 2227 5 3.1134915097072557 2227 6 0.008183377649586987 2227 7 0.23388850105164494 2227 8 0.2905920325581661 2227 9 0.30399961329815284 2227 10-11 0.4016161305428767 2227 12-13 0.3035615518043855 2227 14-15 0.23373679605178665 2227 16-17 0.25192366635595675 2227 18-19 0.18418277729708166 2227 20-21 0.1696230840208912 2227 22-23 0.21441681825125158 2227 24-25 0.2868598692446014 2227 26-27 0.2930266671724553 2227 28-29 0.0940127927047314 2227 30-31 0.19846322964494334 2227 32-33 0.16987486885125946 2227 34-35 0.1341705106700175 2227 36-37 0.30966300919898515 2227 38-39 0.22696413692721507 2227 40-41 0.4378806680813412 2227 42-43 0.5529906606129202 2227 44-45 0.43342675291272315 2227 46-47 0.4184519617387181 2227 48-49 0.3949156456509968 2227 50-51 0.34957183610647036 2227 52-53 0.3053843350761767 2227 54-55 0.26521280259505886 2227 56-57 0.49806911298924916 2227 58-59 0.5296386257925363 2227 60-61 0.39425465680000826 2227 62-63 0.4597315320835591 2227 64-65 0.23071195564218527 2227 66-67 0.29297429084484605 2227 68-69 0.3700082438255592 2227 70-71 0.47187918358403635 2227 72-73 0.39416550880851986 2227 74-75 0.39665427789476837 2227 76-77 0.6657899587101284 2227 78-79 0.49236734261223347 2227 80-81 0.42104480700998437 2227 82-83 0.2509273443442055 2227 84-85 0.24067488068978804 2227 86-87 0.20773630781773988 2227 88-89 0.49699842059177257 2227 90-91 0.3522192524405625 2227 92-93 0.42287237233468034 2227 94-95 0.4327087384330497 2227 96-97 0.2742868946410866 2227 98-99 0.614293310253764 2227 100 0.5117067581571817 2228 1 1.2735627279152393 2228 2 1.2186316638222348 2228 3 -0.16722954854651206 2228 4 -0.06685659440264757 2228 5 3.2005467869034554 2228 6 0.2570929885050468 2228 7 0.36171676407084874 2228 8 0.4626004367396348 2228 9 0.3262393902669345 2228 10-11 0.2922470518171991 2228 12-13 0.25700332559490136 2228 14-15 0.19075752845299832 2228 16-17 0.24471078313614925 2228 18-19 0.26073089418223105 2228 20-21 0.17935989172091382 2228 22-23 0.2819357801396478 2228 24-25 0.3792945407283881 2228 26-27 0.45254756990382106 2228 28-29 0.2538659773260292 2228 30-31 0.25399288274228127 2228 32-33 0.2572832358363044 2228 34-35 0.18275050967565676 2228 36-37 0.3867683358369618 2228 38-39 0.21319372723257857 2228 40-41 0.2678318892103775 2228 42-43 0.4671497913215177 2228 44-45 0.4151785017890077 2228 46-47 0.24562993188214932 2228 48-49 0.3123201640954463 2228 50-51 0.24701863325523732 2228 52-53 0.21905162597980876 2228 54-55 0.29409858564417135 2228 56-57 0.39706029817402566 2228 58-59 0.4731441675068524 2228 60-61 0.26156286965928643 2228 62-63 0.3320960643753921 2228 64-65 0.46371024497510405 2228 66-67 0.42905214921486845 2228 68-69 0.40661642740646897 2228 70-71 0.36539908827281664 2228 72-73 0.4160758580681829 2228 74-75 0.4700952967084291 2228 76-77 0.44259375495476405 2228 78-79 0.5936961939609731 2228 80-81 0.5169278813494245 2228 82-83 0.3893730679527039 2228 84-85 0.5546478451524806 2228 86-87 0.5178287832763928 2228 88-89 0.4118753317420385 2228 90-91 0.48358126752905406 2228 92-93 0.4996775496345549 2228 94-95 0.6286135413748895 2228 96-97 0.5340248071853111 2228 98-99 0.7189706681293728 2228 100 0.4678175592981191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 28.0 13 29.0 14 114.0 15 433.0 16 1660.0 17 4060.0 18 6698.0 19 8552.0 20 10295.0 21 11638.0 22 13556.0 23 15424.0 24 17675.0 25 20107.0 26 22603.0 27 25737.0 28 29291.0 29 33383.0 30 38300.0 31 43667.0 32 51696.0 33 61923.0 34 75634.0 35 98477.0 36 137897.0 37 227026.0 38 560037.0 39 1066839.0 40 1213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.009516587230795 19.45884069473968 18.749746237437932 35.781896480591584 2 19.590169232156548 49.05224651459626 15.19041181617952 16.167172437067677 3 39.935495156331754 16.331126412156074 24.151816259493064 19.581562172019108 4 18.11801274926548 15.88189901516723 21.588340830776566 44.411747404790724 5 17.752376942343474 34.97804946764541 26.271211365979465 20.998362224031652 6 17.640573190629073 28.20295109272784 30.342276601475547 23.81419911516754 7 20.523283353818435 26.605848624918345 35.67228536311258 17.198582658150645 8 21.27665255929585 28.015760110712417 33.59673714160106 17.110850188390675 9 21.020343716234414 28.073616326985533 27.679265260883163 23.22677469589689 10-11 19.65605930668516 30.13198183276109 28.681106597853244 21.530852262700503 12-13 24.427165409180834 26.543522580565266 27.328954578806748 21.700357431447156 14-15 21.722068024978405 27.29640803841498 28.544767166461817 22.4367567701448 16-17 20.971794804318282 29.364854844751843 28.429209533156452 21.23414081777343 18-19 21.459296313610878 29.387165285341442 29.84740277833677 19.306135622710908 20-21 22.871433038492377 28.82357994916393 29.88010411796941 18.424882894374285 22-23 21.799428945600454 30.0897796897204 28.8796559741671 19.231135390512044 24-25 22.238013894779858 28.263167997424137 26.734660943222732 22.764157164573266 26-27 20.245418716466617 26.561614741841304 29.98474840479382 23.208218136898257 28-29 19.865560339080982 27.946549214981747 30.587838278441687 21.600052167495587 30-31 20.237338443761583 30.004685075277603 28.1862720908266 21.57170439013421 32-33 20.78117888909873 29.869539069780405 28.722824993266233 20.626457047854636 34-35 21.36645546890238 29.15353453106666 29.751156350329254 19.7288536497017 36-37 21.771797281106135 27.981394679240495 28.93224901625082 21.31455902340255 38-39 21.317782882313367 29.251755966046904 29.04463333935119 20.38582781228854 40-41 20.93662126087189 29.555317849593532 29.8830321545474 19.62502873498718 42-43 20.557285654079795 29.847379381258975 29.213301604612095 20.382033360049135 44-45 20.758326651166104 30.05078963092765 28.715143080938333 20.475740636967917 46-47 19.861401795085342 27.987332542810528 29.031937224645738 23.119328437458396 48-49 20.956953427100395 28.15043545026455 30.221378394360354 20.6712327282747 50-51 21.967502221369106 30.52437855844755 28.227931820222352 19.28018739996099 52-53 20.35237109667672 30.137459983776786 28.40393319999752 21.10623571954897 54-55 22.242218824154648 28.48113282222974 28.070216522620818 21.206431830994802 56-57 21.399425666058427 28.635069591400047 29.424386879455476 20.54111786308605 58-59 21.143286995106074 27.862504122135984 29.1849163109631 21.809292571794835 60-61 19.704456374342875 30.21199047672122 29.605620623037915 20.477932525897995 62-63 20.477372444714174 31.050322332934932 27.402847274258573 21.06945794809232 64-65 19.81235980972048 30.176668777099586 28.84063316083511 21.170338252344827 66-67 20.263452828027333 30.236935718067237 29.476136149028324 20.023475304877106 68-69 20.83415892928461 29.93078151944743 29.280179659998947 19.954879891269016 70-71 19.798629407521386 30.76079569905789 29.15492772423444 20.285647169186284 72-73 20.079570679785384 30.765052678181664 28.395869646655253 20.759506995377695 74-75 20.104493357564575 29.021645577850087 30.4158255907913 20.458035473794038 76-77 19.496364539828296 30.430415419242784 28.735731379973313 21.337488660955607 78-79 19.630130433840353 29.294924287691295 28.574062922795427 22.50088235567293 80-81 20.679988204270455 29.605423558796375 28.347367207097875 21.3672210298353 82-83 20.367148969501454 28.2179666190917 29.764004687321012 21.650879724085833 84-85 20.78744825835374 28.790801209910867 28.41461970470497 22.007130827030423 86-87 21.560012569698358 28.016688906157604 29.535753206666275 20.887545317477763 88-89 20.736480608299097 27.636618070024983 29.395950142260503 22.230951179415417 90-91 20.21294957569528 27.90600744893947 29.06599556035777 22.815047415007477 92-93 20.110588577673617 28.37516524818962 29.78308369375757 21.731162480379197 94-95 20.449366716305622 28.08882535240047 29.54813714593544 21.91367078535847 96-97 20.191413131310004 28.62474032427345 29.5317284264038 21.65211811801275 98-99 21.185030758609162 28.910480373004248 28.79174935526116 21.11273951312543 100 20.69994798745507 30.151641336350888 27.459372939235106 21.689037736958937 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 8.5 2 17.5 3 16.5 4 23.0 5 26.5 6 23.5 7 30.0 8 42.0 9 53.0 10 61.5 11 81.5 12 97.0 13 103.0 14 120.0 15 166.5 16 243.0 17 286.5 18 406.0 19 690.5 20 1092.0 21 1686.0 22 2614.5 23 4044.0 24 6200.5 25 9297.0 26 13331.5 27 18039.5 28 23505.0 29 29750.0 30 37017.0 31 45071.5 32 53979.0 33 64239.5 34 75627.5 35 87862.5 36 99645.0 37 109016.0 38 115593.0 39 121192.0 40 125214.5 41 125555.5 42 123591.5 43 121557.5 44 120496.5 45 118141.5 46 110476.0 47 101035.0 48 94256.0 49 90722.0 50 87745.5 51 80870.5 52 70901.0 53 60412.5 54 50596.5 55 40935.0 56 31575.0 57 23321.5 58 16570.0 59 12113.0 60 9586.5 61 8119.0 62 6776.0 63 5169.5 64 3884.5 65 3319.0 66 3475.5 67 3300.0 68 2350.0 69 1970.5 70 2374.0 71 2323.5 72 1515.5 73 732.0 74 373.0 75 240.0 76 187.5 77 142.0 78 95.0 79 65.5 80 49.5 81 41.0 82 33.0 83 30.5 84 25.0 85 18.5 86 19.5 87 17.5 88 18.5 89 15.5 90 7.5 91 11.5 92 12.0 93 8.0 94 10.0 95 12.5 96 17.5 97 24.0 98 37.5 99 79.0 100 87.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.357039805076797 2 0.0056114724813389515 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.9024857662098024E-4 30-31 0.009887801510221395 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.998470583500258E-4 40-41 0.001315793547348444 42-43 1.160994306483921E-4 44-45 0.0 46-47 0.0018575908903742736 48-49 0.0 50-51 0.0 52-53 0.0015479924086452279 54-55 0.019253155582525022 56-57 0.011745392400595666 58-59 0.014705927882129665 60-61 3.095984817290456E-4 62-63 0.012693537750890868 64-65 1.547992408645228E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.321988612967842E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2583992.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.51194034951027 #Duplication Level Percentage of deduplicated Percentage of total 1 86.22880058610644 64.2507524568178 2 8.591987793489904 12.804113639044806 3 2.352221029274408 5.258056590664752 4 0.9980408519356604 2.974638417032174 5 0.5451871951990933 2.0311477883995828 6 0.3096749378683475 1.3844688298910766 7 0.21307426134967647 1.111360365619214 8 0.13991588568171523 0.8340323302291891 9 0.10839478727687794 0.7269035331395326 >10 0.48141116145583734 6.052539892063821 >50 0.020934008724298517 1.0512991471011222 >100 0.0099872792844885 1.319782215536448 >500 3.7022235324885087E-4 0.2009047944604952 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.86998102161307E-5 0.0 3 0.0 0.0 0.0 3.86998102161307E-5 0.0 4 0.0 0.0 0.0 3.86998102161307E-5 0.0 5 0.0 0.0 0.0 3.86998102161307E-5 0.0 6 0.0 0.0 0.0 3.86998102161307E-5 0.0 7 0.0 0.0 0.0 3.86998102161307E-5 0.0 8 0.0 0.0 0.0 3.86998102161307E-5 0.0 9 0.0 0.0 0.0 3.86998102161307E-5 0.0 10-11 0.0 0.0 0.0 3.86998102161307E-5 0.0 12-13 0.0 0.0 0.0 3.86998102161307E-5 0.0 14-15 0.0 0.0 0.0 3.86998102161307E-5 0.0 16-17 0.0 0.0 0.0 3.86998102161307E-5 0.0 18-19 0.0 0.0 0.0 3.86998102161307E-5 0.0 20-21 0.0 0.0 0.0 3.86998102161307E-5 0.0 22-23 0.0 0.0 0.0 3.86998102161307E-5 0.0 24-25 0.0 0.0 0.0 3.86998102161307E-5 0.0 26-27 0.0 0.0 0.0 3.86998102161307E-5 0.0 28-29 0.0 0.0 0.0 3.86998102161307E-5 0.0 30-31 0.0 0.0 0.0 3.86998102161307E-5 0.0 32-33 0.0 0.0 0.0 3.86998102161307E-5 0.0 34-35 0.0 0.0 0.0 3.86998102161307E-5 0.0 36-37 0.0 0.0 0.0 3.86998102161307E-5 0.0 38-39 0.0 0.0 0.0 3.86998102161307E-5 0.0 40-41 0.0 0.0 0.0 3.86998102161307E-5 0.0 42-43 0.0 0.0 0.0 3.86998102161307E-5 0.0 44-45 0.0 0.0 0.0 3.86998102161307E-5 0.0 46-47 1.934990510806535E-5 0.0 0.0 3.86998102161307E-5 0.0 48-49 7.73996204322614E-5 0.0 0.0 3.86998102161307E-5 0.0 50-51 7.73996204322614E-5 0.0 0.0 3.86998102161307E-5 0.0 52-53 1.547992408645228E-4 0.0 0.0 3.86998102161307E-5 0.0 54-55 1.547992408645228E-4 0.0 0.0 3.86998102161307E-5 0.0 56-57 1.7414914597258815E-4 0.0 1.160994306483921E-4 3.86998102161307E-5 0.0 58-59 2.321988612967842E-4 0.0 2.321988612967842E-4 3.86998102161307E-5 0.0 60-61 2.708986715129149E-4 0.0 2.321988612967842E-4 3.86998102161307E-5 0.0 62-63 2.708986715129149E-4 0.0 2.321988612967842E-4 3.86998102161307E-5 0.0 64-65 3.482982919451763E-4 0.0 2.321988612967842E-4 3.86998102161307E-5 0.0 66-67 3.86998102161307E-4 0.0 2.321988612967842E-4 5.804971532419605E-5 0.0 68-69 4.0634800726937234E-4 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 70-71 4.643977225935684E-4 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 72-73 4.643977225935684E-4 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 74-75 5.224474379177644E-4 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 76-77 6.57896773674222E-4 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 78-79 8.900956349710061E-4 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 80-81 0.0012577438320242476 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 82-83 0.001431892977996836 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 84-85 0.0015092925984290973 0.0 2.321988612967842E-4 7.73996204322614E-5 0.0 86-87 0.0016253920290774894 3.86998102161307E-5 2.321988612967842E-4 7.73996204322614E-5 0.0 88 0.0016253920290774894 3.86998102161307E-5 2.321988612967842E-4 7.73996204322614E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 9520 0.0 78.59336 8 CACCGGC 9330 0.0 75.96785 9 CTTCACC 10605 0.0 73.11961 6 GTGTGGC 4020 0.0 70.7589 7 CGCTGCA 60 1.2551027E-10 70.64836 1 CCTTCAC 11670 0.0 69.42317 5 CGTAGTG 2260 0.0 68.53936 4 GCAGCCT 70 7.2759576E-12 68.125206 1 TTCACCG 11940 0.0 64.551056 7 CAGCTGC 100 0.0 63.583527 1 CGGGGCA 1175 0.0 63.51756 94 CAGCGTA 1110 0.0 59.669224 1 GCCTTCA 3420 0.0 59.291466 4 GCGTAGT 1445 0.0 58.79566 3 ACCTTCA 4540 0.0 58.105198 4 TGTGGCA 4760 0.0 58.082172 8 TGTGTGG 4605 0.0 57.794697 6 GCTGCGG 65 7.4578566E-10 57.774525 2 CGGGCCC 670 0.0 57.447823 7 AGCAGCA 110 0.0 55.476673 2 >>END_MODULE