FastQCFastQC Report
Tue 7 Mar 2017
1SN_12A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_12A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3423902
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA157540.46011830946096005No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG87750.25628654091150976No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG74750.21831816448017494No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG66910.19542031284773922No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT66790.19506983552683457No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66270.19355110046958118No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA65760.19206157185573652No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA63420.18522726409809626No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG56950.16633069521265503No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG56050.16370211530587034No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC54230.15838654260548346No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC48330.14115474099433922No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG48260.14095029589047817No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46840.13680298092644008No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT46350.13537186519941283No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC45690.13344423993443738No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA43790.12789501568678077No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA43510.12707723527133663No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA43480.12698961594111047No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA41290.120593404834601No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA39250.1146352903792223No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA38240.11168543959494168No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA35730.10435462229935319No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA35360.1032739838932306No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG34530.1008498490903069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAT900.087.9996953
AGCTGCG900.087.9996952
GCAGCTC450.087.9996953
GCAGCTA550.087.9996953
GCAGCCC356.0754246E-1087.9996953
AGCTGCA1850.087.999692
GCAGCTT600.087.999693
CAGCTGC7000.087.3711241
GCTGCCC1900.085.683913
CAGCAGC5800.084.9652251
CTGCAGC2750.084.7997061
AGCTGCC2550.084.548732
TGCAGCT1250.084.47972
AGCAGCC1000.083.599712
CTGCTGC2700.083.1108251
GCTGCTT700.081.7140053
GCTGCTA700.081.7140053
CAGCCCT177500.081.678591
CAGCGTA71600.081.54721
GCTGCCA650.081.2304843
Produced by FastQC (version 0.11.5)