##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_12A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3423902
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.777433758326026	12.0	12.0	12.0	2.0	27.0
2	14.66440306994768	12.0	12.0	12.0	12.0	22.0
3	13.706288614568992	12.0	12.0	12.0	12.0	22.0
4	13.829173264889008	12.0	12.0	12.0	12.0	22.0
5	22.436589598650897	22.0	22.0	22.0	22.0	27.0
6	26.871347369171197	27.0	22.0	32.0	22.0	32.0
7	34.96583985172473	37.0	37.0	37.0	27.0	41.0
8	37.59661900369812	41.0	37.0	41.0	27.0	41.0
9	38.52723938944514	41.0	41.0	41.0	32.0	41.0
10-11	38.873887015457804	41.0	41.0	41.0	37.0	41.0
12-13	39.0605820493694	41.0	41.0	41.0	37.0	41.0
14-15	39.014186591789134	41.0	41.0	41.0	37.0	41.0
16-17	38.92428550817167	41.0	41.0	41.0	37.0	41.0
18-19	38.860866344889544	41.0	41.0	41.0	37.0	41.0
20-21	38.87301607931535	41.0	41.0	41.0	37.0	41.0
22-23	38.856400387627914	41.0	41.0	41.0	37.0	41.0
24-25	38.88066845955287	41.0	41.0	41.0	37.0	41.0
26-27	38.8447252579075	41.0	41.0	41.0	37.0	41.0
28-29	38.790775553739564	41.0	41.0	41.0	34.5	41.0
30-31	38.661958198569934	41.0	41.0	41.0	32.0	41.0
32-33	38.70721445882505	41.0	41.0	41.0	32.0	41.0
34-35	38.67995535503061	41.0	41.0	41.0	32.0	41.0
36-37	38.60745474023497	41.0	41.0	41.0	32.0	41.0
38-39	38.516522085036314	41.0	41.0	41.0	32.0	41.0
40-41	38.558183616236676	41.0	41.0	41.0	32.0	41.0
42-43	38.30564601440111	41.0	41.0	41.0	32.0	41.0
44-45	38.41314178968908	41.0	41.0	41.0	32.0	41.0
46-47	38.429426280308256	41.0	41.0	41.0	32.0	41.0
48-49	38.386858181104486	41.0	41.0	41.0	32.0	41.0
50-51	38.262762485608526	41.0	41.0	41.0	32.0	41.0
52-53	38.231610454972135	41.0	41.0	41.0	32.0	41.0
54-55	38.10278740454604	41.0	37.0	41.0	32.0	41.0
56-57	37.888837355742076	41.0	37.0	41.0	29.5	41.0
58-59	37.886990632325336	41.0	37.0	41.0	29.5	41.0
60-61	37.88377660926042	41.0	37.0	41.0	29.5	41.0
62-63	37.859585642346076	41.0	37.0	41.0	29.5	41.0
64-65	37.837891972375374	41.0	37.0	41.0	27.0	41.0
66-67	37.73215895197935	41.0	37.0	41.0	27.0	41.0
68-69	37.74309355232714	41.0	37.0	41.0	27.0	41.0
70-71	37.711713419367726	41.0	37.0	41.0	27.0	41.0
72-73	37.58222796680512	41.0	37.0	41.0	27.0	41.0
74-75	37.48386869717649	41.0	37.0	41.0	27.0	41.0
76-77	36.44161515136824	39.0	34.5	41.0	24.5	41.0
78-79	36.959893419846715	41.0	37.0	41.0	27.0	41.0
80-81	37.60224796153628	41.0	37.0	41.0	27.0	41.0
82-83	37.78283972496877	41.0	37.0	41.0	27.0	41.0
84-85	37.684561211156165	41.0	37.0	41.0	27.0	41.0
86-87	37.53173002615145	41.0	37.0	41.0	27.0	41.0
88-89	37.440235731046045	41.0	37.0	41.0	27.0	41.0
90-91	37.26802183590535	41.0	37.0	41.0	27.0	41.0
92-93	37.19158404650601	41.0	37.0	41.0	27.0	41.0
94-95	37.016180661712866	41.0	37.0	41.0	27.0	41.0
96-97	36.960583714136675	41.0	37.0	41.0	27.0	41.0
98-99	36.83540811039568	41.0	37.0	41.0	27.0	41.0
100	36.705580942445195	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5234871392756517
1101	2	-0.50674013157135
1101	3	0.0858331564574879
1101	4	-0.017300590822786788
1101	5	-1.0272100802953368
1101	6	-0.9182903178320174
1101	7	-0.4236725216428354
1101	8	-0.24366823397541282
1101	9	-0.14474279151531277
1101	10-11	-0.1280546585323492
1101	12-13	-0.06395068648824775
1101	14-15	0.030200715289815605
1101	16-17	-0.04133682890811485
1101	18-19	0.05247399528324337
1101	20-21	-0.045040752139520635
1101	22-23	0.05466776444417576
1101	24-25	0.057508973759667015
1101	26-27	-0.017755779614681444
1101	28-29	-0.1101871021848595
1101	30-31	-0.06836283327118053
1101	32-33	-0.06825492650512643
1101	34-35	0.07471409067910173
1101	36-37	0.09204119673395184
1101	38-39	-0.05399070165856301
1101	40-41	0.11338089029377585
1101	42-43	0.14336722014789416
1101	44-45	0.06805674783361582
1101	46-47	0.01522477430984992
1101	48-49	0.04401466163015044
1101	50-51	-0.07363067063820239
1101	52-53	0.1135448526901115
1101	54-55	0.04502464169642195
1101	56-57	0.20624922335122164
1101	58-59	0.14847425021518035
1101	60-61	0.0751330404257331
1101	62-63	-0.01733215176326297
1101	64-65	0.02977752725752225
1101	66-67	-0.08784133138340877
1101	68-69	0.06493943299109617
1101	70-71	0.0367699576492484
1101	72-73	0.1356082253343729
1101	74-75	0.09616494282715848
1101	76-77	-0.2798825285475033
1101	78-79	-0.027857944778943988
1101	80-81	0.07859342490601762
1101	82-83	0.01975860984446598
1101	84-85	0.04410903245241116
1101	86-87	0.025577272440763466
1101	88-89	-0.2245315542751598
1101	90-91	-0.10420283072576098
1101	92-93	0.11772591501815555
1101	94-95	0.021519696174806313
1101	96-97	-0.034048152528548314
1101	98-99	-0.1479665091354505
1101	100	-0.17797411821410947
1102	1	1.7752349396352738
1102	2	0.7942480859169887
1102	3	-0.011347564722884229
1102	4	-0.06283855079129985
1102	5	-0.7982510481270708
1102	6	-0.23197297675166695
1102	7	-0.16123585644736949
1102	8	-0.34366523508656144
1102	9	-0.35422130927046425
1102	10-11	-0.40308782746934213
1102	12-13	-0.284992819315832
1102	14-15	-0.2903349620671989
1102	16-17	-0.2247979234534938
1102	18-19	-0.3348481630828388
1102	20-21	-0.15861357814856092
1102	22-23	-0.18285025257902987
1102	24-25	-0.18681462574530627
1102	26-27	-0.17414967262837422
1102	28-29	-0.22579961854356867
1102	30-31	-0.1325086755904934
1102	32-33	-0.292368802230051
1102	34-35	-0.08704157033676552
1102	36-37	-0.2952551533507588
1102	38-39	-0.35852914646602585
1102	40-41	-0.3705600041710895
1102	42-43	-0.22179694003111905
1102	44-45	-0.195135994097285
1102	46-47	-0.460842866352813
1102	48-49	-0.3898568849235886
1102	50-51	-0.24538377981146908
1102	52-53	-0.3274716859031912
1102	54-55	-0.3172078537949332
1102	56-57	-0.26357532158411345
1102	58-59	-0.2259932658542425
1102	60-61	-0.1965763228744919
1102	62-63	-0.4748244630693321
1102	64-65	-0.483535476805244
1102	66-67	-0.49026322955965185
1102	68-69	-0.5445611472214296
1102	70-71	-0.49549942417495885
1102	72-73	-0.3602402645578664
1102	74-75	-0.7383506290425501
1102	76-77	-0.42695110354798516
1102	78-79	-0.4186066402344224
1102	80-81	-0.43559304734048965
1102	82-83	-0.507328379894318
1102	84-85	-0.5144380850524186
1102	86-87	-0.44396427326235965
1102	88-89	-0.5615668616763116
1102	90-91	-0.5053714386248345
1102	92-93	-0.2551754898776579
1102	94-95	-0.2555110783333916
1102	96-97	-0.4470719031708299
1102	98-99	-0.5200316811217292
1102	100	-0.7188507012288738
1103	1	1.4740582385538854
1103	2	0.8326450519294575
1103	3	0.3787214768309113
1103	4	1.1551606513911352
1103	5	2.8520933157525477
1103	6	-0.014308137219511963
1103	7	-0.5349362953382624
1103	8	-0.17561846719422647
1103	9	-0.29500468036214755
1103	10-11	-0.3029179320135498
1103	12-13	-0.448054652914081
1103	14-15	-0.26809249030157645
1103	16-17	-0.2901398206673136
1103	18-19	-0.32326167616917445
1103	20-21	-0.2724892061904072
1103	22-23	-0.09770974157893875
1103	24-25	-0.28844661654189707
1103	26-27	-0.18359108401280366
1103	28-29	-0.2512188776041526
1103	30-31	-0.18605870146596715
1103	32-33	-0.3542774952256096
1103	34-35	-0.3940223485897789
1103	36-37	-0.36681930912725846
1103	38-39	-0.25924020308164586
1103	40-41	-0.2950424548075574
1103	42-43	0.04785507648812626
1103	44-45	-0.5891593415509462
1103	46-47	-0.5380209390965334
1103	48-49	-0.22012376379421283
1103	50-51	-0.31805850539347347
1103	52-53	-0.13139661533008962
1103	54-55	-0.26222777756662907
1103	56-57	-0.3708323481604765
1103	58-59	-0.4552058760245643
1103	60-61	-0.24360450946344514
1103	62-63	-0.30687109320243167
1103	64-65	-0.4495657938727291
1103	66-67	-0.3577545243847098
1103	68-69	-0.3420890531601515
1103	70-71	-0.6102666669443764
1103	72-73	-0.3739095616504855
1103	74-75	-0.42987194717036203
1103	76-77	-0.3244257304177438
1103	78-79	-0.5332718180065612
1103	80-81	-0.2596756996893248
1103	82-83	-0.5198176003480981
1103	84-85	-0.4171933496105922
1103	86-87	-0.24758580165547528
1103	88-89	-0.3925192866152116
1103	90-91	-0.6506958582057152
1103	92-93	-0.272427325394041
1103	94-95	-0.16206612580673152
1103	96-97	-0.28804237821896805
1103	98-99	-0.4126674007689175
1103	100	-0.18466383082450477
1104	1	1.6459291350095118
1104	2	-0.7062733881487588
1104	3	0.030400723731439427
1104	4	0.008430491624206837
1104	5	-1.1557147298819288
1104	6	-0.5347440433433412
1104	7	-0.23200031777449226
1104	8	-0.2552277383299
1104	9	-0.2534987535683584
1104	10-11	-0.21186738383105563
1104	12-13	-0.18559790809892007
1104	14-15	-0.20523112325403048
1104	16-17	-0.29300558297233437
1104	18-19	-0.24829804250232002
1104	20-21	-0.21659833648734406
1104	22-23	-0.13207167402260467
1104	24-25	-0.17413556294560806
1104	26-27	-0.18799280510310723
1104	28-29	-0.16211557816736644
1104	30-31	-0.17701780652078725
1104	32-33	-0.38031055082765874
1104	34-35	-0.2917795425920886
1104	36-37	-0.33787966839533823
1104	38-39	-0.24040489188245573
1104	40-41	-0.09880135248241828
1104	42-43	-0.2575431977949947
1104	44-45	-0.25322117180147075
1104	46-47	-0.5039985164795979
1104	48-49	-0.48736392311209187
1104	50-51	-0.44959713126996803
1104	52-53	-0.3848461440305755
1104	54-55	-0.31043238972938525
1104	56-57	-0.14308146808841116
1104	58-59	-0.24704246769969984
1104	60-61	-0.32581113283070096
1104	62-63	-0.34221889686642015
1104	64-65	-0.36378104990667737
1104	66-67	-0.6297668719284744
1104	68-69	-0.45402251739867694
1104	70-71	-0.2870750840665508
1104	72-73	-0.31573091280434795
1104	74-75	-0.42939159859786713
1104	76-77	-0.45432838158806277
1104	78-79	-0.550360932872465
1104	80-81	-0.3440411701111401
1104	82-83	-0.35994521249860156
1104	84-85	-0.3395969839306119
1104	86-87	-0.34617088254589845
1104	88-89	-0.2849701937664122
1104	90-91	-0.352747749459148
1104	92-93	-0.3606189150756691
1104	94-95	-0.3041571117187729
1104	96-97	-0.3345933257847662
1104	98-99	-0.5835187471737129
1104	100	-0.3025308926561152
1105	1	1.418483187140147
1105	2	0.31474774120620097
1105	3	-0.033182912469611026
1105	4	-0.2138720070317195
1105	5	-1.1955138762770652
1105	6	0.42655765050762895
1105	7	-0.027597459656512058
1105	8	-0.10718473056761013
1105	9	0.12702968910453905
1105	10-11	-0.007322520600887117
1105	12-13	-0.012141396535945148
1105	14-15	-0.07520888125240077
1105	16-17	0.0195395793540456
1105	18-19	-0.007733292871868969
1105	20-21	-0.020102506671342724
1105	22-23	-0.11599224604849212
1105	24-25	-0.05375890067136879
1105	26-27	-0.08916233034689469
1105	28-29	-0.09776086185947719
1105	30-31	0.017745429744017827
1105	32-33	-0.2040121332609175
1105	34-35	-0.020056805223276797
1105	36-37	-0.1760113315124201
1105	38-39	0.03113976517246897
1105	40-41	0.08605552407413342
1105	42-43	0.08191827378026773
1105	44-45	-0.052182435561697105
1105	46-47	-0.23621912661401723
1105	48-49	-0.1548206366962006
1105	50-51	-0.056724957078778004
1105	52-53	-0.09792568941307422
1105	54-55	-0.1245428331941909
1105	56-57	-0.03172573795802691
1105	58-59	-0.09532962805441514
1105	60-61	-0.2026298422611319
1105	62-63	-0.31253851659443654
1105	64-65	-0.23831324746148397
1105	66-67	-0.16136109631054296
1105	68-69	-0.3376139633343058
1105	70-71	-0.3652232630668948
1105	72-73	-0.3059604363558819
1105	74-75	-0.28525515256495027
1105	76-77	-0.2752585686390745
1105	78-79	-0.03279985263662155
1105	80-81	-0.3037086573246768
1105	82-83	-0.20673700108305582
1105	84-85	-0.18319207236842772
1105	86-87	-0.2723704759237151
1105	88-89	-0.27387182193865556
1105	90-91	-0.32069061464787296
1105	92-93	-0.15826217680737642
1105	94-95	-0.099329112814587
1105	96-97	-0.27784391791001184
1105	98-99	-0.4733497272013878
1105	100	-0.47345323061779254
1106	1	1.2663075884495036
1106	2	-0.5775373558021322
1106	3	-0.06504263542457522
1106	4	-0.21922377151759243
1106	5	-1.1081790093793984
1106	6	-0.562264459577559
1106	7	-0.09755201527001134
1106	8	-0.23731698908824228
1106	9	-0.24940612697112385
1106	10-11	-0.03316911044743165
1106	12-13	-0.15130323723884231
1106	14-15	-0.09315211605954232
1106	16-17	-0.024433452010029555
1106	18-19	0.11787239155344054
1106	20-21	-0.06088918564301338
1106	22-23	-0.08064019268897482
1106	24-25	-0.12560329811894633
1106	26-27	-0.07607445346179986
1106	28-29	-0.21370106197289118
1106	30-31	0.02081091131920232
1106	32-33	-0.04238048365553482
1106	34-35	-0.09215852827858129
1106	36-37	-0.4087142614514434
1106	38-39	-0.1544288674060894
1106	40-41	-0.16406382635544503
1106	42-43	0.04136687479557821
1106	44-45	-0.10338909121062301
1106	46-47	-0.43742418585592446
1106	48-49	-0.32402837790189665
1106	50-51	-0.16888585362096364
1106	52-53	-0.2195015864037657
1106	54-55	-0.2220487132933826
1106	56-57	-0.3626620534810314
1106	58-59	-0.2913319207989673
1106	60-61	-0.1307751821970058
1106	62-63	-0.23871647492669723
1106	64-65	-0.4655089532786363
1106	66-67	-0.35357914422403525
1106	68-69	-0.3354048400962455
1106	70-71	-0.5262973105060311
1106	72-73	-0.35529929579147534
1106	74-75	-0.4376067361246996
1106	76-77	-0.49023986026919175
1106	78-79	-0.29282680910944947
1106	80-81	-0.2500659605827096
1106	82-83	-0.3396641121792996
1106	84-85	-0.46297608610056074
1106	86-87	-0.4332529545204622
1106	88-89	-0.5265313600144879
1106	90-91	-0.526449995059501
1106	92-93	-0.35663437378271823
1106	94-95	-0.4008003159125195
1106	96-97	-0.32629715154646277
1106	98-99	-0.5584150354523771
1106	100	-0.400224520225791
1107	1	1.4228703364263833
1107	2	-0.5976099223278073
1107	3	-0.15235775465736445
1107	4	-0.18760484543152067
1107	5	-1.1736939693836383
1107	6	-0.27847718386205145
1107	7	-0.41011469433943404
1107	8	-0.1877647894031682
1107	9	-0.27147766381011706
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2226	100	0.4659420648385648
2227	1	1.0908986887971697
2227	2	1.8639608457100163
2227	3	0.4967699936453105
2227	4	-0.0813414998574089
2227	5	3.1745255773737853
2227	6	0.1561952645802691
2227	7	0.24967161156225615
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2227	9	0.164535458453976
2227	10-11	0.11635055604579492
2227	12-13	0.14660078748823224
2227	14-15	0.17259168766948818
2227	16-17	0.10823540433887047
2227	18-19	0.15994634421809195
2227	20-21	0.23175662599673075
2227	22-23	0.1272013264720755
2227	24-25	0.1789259457183192
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2227	28-29	0.19118307081655672
2227	30-31	0.3008339387776999
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2227	36-37	0.23021986845930797
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2227	48-49	0.17407512900386024
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2227	84-85	0.34581245049895415
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2227	90-91	0.24703509078511132
2227	92-93	0.24478849199409325
2227	94-95	0.39313126198051407
2227	96-97	0.23631450596694492
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2227	100	0.465684577054283
2228	1	1.3285035911850809
2228	2	1.6950082424559643
2228	3	-0.11388566151694057
2228	4	-0.13293169126550985
2228	5	3.217064774322619
2228	6	0.22390149443323537
2228	7	0.30962887149121343
2228	8	0.5005283400863334
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2228	10-11	0.26832267323754166
2228	12-13	0.26122214247144626
2228	14-15	0.3194891634400463
2228	16-17	0.18311729143172784
2228	18-19	0.11401030804167078
2228	20-21	0.17364756673411108
2228	22-23	0.24436710431063347
2228	24-25	0.28391870047065737
2228	26-27	0.204314194324283
2228	28-29	0.2223568751386864
2228	30-31	0.1261579608812724
2228	32-33	0.2468977205794971
2228	34-35	0.2049243762894406
2228	36-37	0.16690875317878096
2228	38-39	0.09257329080138277
2228	40-41	0.2356444001860254
2228	42-43	0.5397770876123147
2228	44-45	0.30748546218104167
2228	46-47	0.2596940851919314
2228	48-49	0.26214034577297696
2228	50-51	0.304604519665844
2228	52-53	0.2636782436240779
2228	54-55	0.29029077934676906
2228	56-57	0.4842619149788874
2228	58-59	0.5452925191429969
2228	60-61	0.4198099626031606
2228	62-63	0.47454009610134307
2228	64-65	0.4296242143009579
2228	66-67	0.443537315889607
2228	68-69	0.3251677133849
2228	70-71	0.49942147058610686
2228	72-73	0.47690614510708684
2228	74-75	0.610593088747315
2228	76-77	0.5864299808586111
2228	78-79	0.49698508808914
2228	80-81	0.36032390159454053
2228	82-83	0.4447830522173035
2228	84-85	0.4382112475864375
2228	86-87	0.25615947189702126
2228	88-89	0.3772284589773136
2228	90-91	0.41002030242372456
2228	92-93	0.5442283765789355
2228	94-95	0.755303278046199
2228	96-97	0.45577353454194736
2228	98-99	0.5690554516463138
2228	100	0.629813552002787
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	51.0
14	167.0
15	618.0
16	2354.0
17	5773.0
18	9577.0
19	11987.0
20	14119.0
21	16495.0
22	18920.0
23	21315.0
24	24292.0
25	27781.0
26	30924.0
27	34911.0
28	39633.0
29	45088.0
30	51726.0
31	59165.0
32	69041.0
33	82512.0
34	101090.0
35	130697.0
36	182063.0
37	299845.0
38	738814.0
39	1403188.0
40	1731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.747704312558717	19.364711571529654	18.79360620247377	36.09397791343786
2	17.920822733233834	50.450184156952545	14.627640775649805	17.001352334163816
3	42.162304879053195	15.31045573150166	22.81151738571957	19.715722003725574
4	16.80413166031037	15.948178423331042	23.402772626085678	43.84491729027291
5	17.69682660309787	34.56164925281156	26.62725159773849	21.114272546352087
6	17.67994527880763	28.44742635741327	30.14397024213894	23.728658121640166
7	20.3797304946228	26.660780594771694	35.709520891661036	17.249968018944468
8	21.04219104401937	28.29642904499019	33.16204727822233	17.499332632768112
9	20.98357371209807	28.45548733579407	27.365648899997723	23.19529005211014
10-11	19.670904716314894	30.155755042054356	28.898987178955473	21.274353062675274
12-13	23.877056060599866	26.899776921185243	27.466805416743824	21.75636160147107
14-15	21.87364007497878	27.446652970791803	28.58682871180308	22.09287824242633
16-17	20.6976134246833	29.543748623646355	28.486782039906515	21.27185591176383
18-19	21.289452793917583	29.606776128522373	29.713248217968857	19.39052285959119
20-21	22.65107178885377	28.848197173867714	29.928441293004298	18.572289744274222
22-23	21.545987589598067	30.259087438834403	28.797816058987667	19.397108912579856
24-25	22.11551323606809	28.49815794961421	26.739302117876036	22.647026696441664
26-27	20.054429127936487	26.841670702023595	30.03963022306129	23.064269946978623
28-29	19.79822976438088	28.05182811561513	30.554708972487326	21.595233147516666
30-31	20.119325855069558	29.987777366088842	28.385369467775785	21.507527311065815
32-33	20.89261024410161	29.851058821192893	28.730203142496485	20.526127792209007
34-35	21.357270155512627	29.031861893243438	30.119670481222887	19.491197470021046
36-37	21.931629468366793	28.23455519462882	28.777590596927134	21.056224740077255
38-39	21.44750988135366	29.340618066149066	29.068046624392903	20.14382542810437
40-41	20.859983346267093	29.773950839652475	29.80020777736921	19.56585803671123
42-43	20.5614568080548	29.704826065858008	29.373536846105015	20.360180279982178
44-45	20.599114694287397	30.185866885208746	28.70529004626885	20.50972837423501
46-47	19.943396846374863	28.234692797190284	28.94305001332569	22.87886034310916
48-49	20.98680408884426	28.308656075532546	30.11288438058163	20.591655455041565
50-51	21.860015269128613	30.7481347304917	28.129718666013222	19.26213133436646
52-53	20.3714707670416	30.112874412266077	28.444386939320786	21.07126788137154
54-55	22.13203144683943	28.54847202392472	28.119141602065294	21.20035492717055
56-57	21.27948908300196	28.685860619195346	29.327661540856752	20.706988756945943
58-59	21.120651567627206	28.061317860491165	29.110721015185455	21.707309556696178
60-61	19.705540250547806	30.38804461874224	29.525621021073206	20.380794109636753
62-63	20.31589590618523	31.06962103795579	27.466799397511792	21.147683658347184
64-65	19.833479244048632	30.39014748543144	28.70597031599223	21.070402954527697
66-67	20.062650741756045	30.169744928447134	29.564251546919273	20.20335278287755
68-69	20.891485796030377	30.01882063213258	29.163728985233806	19.925964586603236
70-71	19.60342322881905	30.8777675295613	29.335200014486396	20.183609227133253
72-73	20.02455093632937	30.652571247658372	28.714183408286804	20.608694407725455
74-75	20.041081783298704	29.22329552656589	30.214065706319865	20.521556983815543
76-77	19.648780835432788	30.520528917007557	28.67971980506451	21.150970442495144
78-79	19.610447378458844	29.52955429214972	28.455881622780094	22.404116706611347
80-81	20.64814167133849	29.62713680159432	28.47889823772418	21.245823289343004
82-83	20.415420768468255	28.342224748255063	29.67373190003686	21.568622583239826
84-85	20.572726672667617	29.22710696743073	28.447557786408606	21.75260857349305
86-87	21.473248942288652	28.306023361649952	29.858988370578366	20.36173932548303
88-89	21.21808684944838	28.331652015741106	29.891057045441137	20.55920408936938
90-91	21.745248549754052	28.173922618112318	30.02937876142483	20.0514500707088
92-93	21.989677274641622	28.63877237140549	30.20242693862149	19.169123415331395
94-95	21.86362226488959	28.3547397092557	30.292441197207165	19.489196828647547
96-97	21.40055410464435	28.94660828493339	29.968468723695945	19.684368886726315
98-99	22.43491490118584	29.11000373258347	29.62503015565282	18.83005121057787
100	22.058779719746653	30.05518265417643	28.36684578004861	19.519191846028303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	10.5
2	18.0
3	25.5
4	37.0
5	43.0
6	45.0
7	57.0
8	76.5
9	90.5
10	103.0
11	124.0
12	144.0
13	159.0
14	199.5
15	260.5
16	327.0
17	428.0
18	614.0
19	969.5
20	1496.5
21	2342.5
22	3784.0
23	5898.0
24	8899.5
25	13292.0
26	18939.0
27	25243.0
28	32565.5
29	41178.0
30	51164.0
31	62347.5
32	74903.0
33	89269.0
34	104238.0
35	120345.0
36	136468.5
37	148770.0
38	157402.5
39	163853.5
40	166959.0
41	166403.0
42	163489.0
43	159740.0
44	157271.0
45	154379.0
46	145682.0
47	132904.5
48	122713.0
49	116398.0
50	110391.0
51	101926.5
52	91041.0
53	77803.0
54	63536.5
55	50403.0
56	38963.0
57	28874.0
58	20496.0
59	14747.0
60	11816.5
61	9912.0
62	7919.5
63	6066.5
64	4561.5
65	4224.0
66	5121.5
67	4959.0
68	3358.0
69	3066.0
70	4038.5
71	3811.0
72	2130.5
73	828.0
74	371.0
75	227.0
76	157.0
77	113.5
78	92.0
79	71.0
80	57.0
81	47.5
82	35.5
83	26.5
84	25.0
85	27.5
86	25.0
87	24.0
88	23.5
89	20.5
90	19.0
91	24.0
92	21.5
93	15.5
94	17.5
95	16.0
96	18.0
97	30.5
98	54.5
99	114.0
100	122.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.338233395698825
2	0.005607637134474059
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.1904832556539293E-4
30-31	0.009798761763625244
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.469868588528526E-4
40-41	0.0013142899533923576
42-43	1.606354387479548E-4
44-45	0.0
46-47	0.0018838155998623794
48-49	1.460322170435953E-5
50-51	0.0
52-53	0.0015333382789577505
54-55	0.019407681645093815
56-57	0.011989245019279173
58-59	0.015406398898099303
60-61	1.3142899533923576E-4
62-63	0.012631786774270994
64-65	1.3142899533923576E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.606354387479548E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3423902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.63203324901227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.50368731361655	62.103066598757465
2	8.887019416349682	12.909645794658555
3	2.5608442256271977	5.579979688238871
4	1.0572298593490606	3.071550171843582
5	0.5698083139946127	2.0693168203810166
6	0.3431403763454402	1.495378993428036
7	0.230048500147533	1.1696223218120714
8	0.15670629285323667	0.9105517338276593
9	0.11744535825060883	0.7677265648860341
>10	0.5338627908906722	6.634850415706202
>50	0.02745220663689246	1.362205218505303
>100	0.012059917693553867	1.5492686264716404
>500	6.136131584112618E-4	0.3073110172090678
>1k	8.1815086622392E-5	0.06952603427439222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.920644340871906E-5	0.0	0.0	0.0	0.0
2	2.920644340871906E-5	0.0	0.0	0.0	0.0
3	2.920644340871906E-5	0.0	0.0	0.0	0.0
4	2.920644340871906E-5	0.0	0.0	0.0	0.0
5	2.920644340871906E-5	0.0	0.0	0.0	0.0
6	2.920644340871906E-5	0.0	0.0	0.0	0.0
7	2.920644340871906E-5	0.0	0.0	0.0	0.0
8	2.920644340871906E-5	0.0	0.0	0.0	0.0
9	2.920644340871906E-5	0.0	0.0	0.0	0.0
10-11	2.920644340871906E-5	0.0	0.0	0.0	0.0
12-13	2.920644340871906E-5	0.0	0.0	0.0	0.0
14-15	2.920644340871906E-5	0.0	0.0	0.0	0.0
16-17	2.920644340871906E-5	0.0	0.0	0.0	0.0
18-19	2.920644340871906E-5	0.0	0.0	0.0	0.0
20-21	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
22-23	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
24-25	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
26-27	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
28-29	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
30-31	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
32-33	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
34-35	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
36-37	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
38-39	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
40-41	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
42-43	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
44-45	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
46-47	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
48-49	2.920644340871906E-5	0.0	0.0	2.920644340871906E-5	0.0
50-51	8.761933022615717E-5	0.0	0.0	2.920644340871906E-5	0.0
52-53	1.1682577363487623E-4	0.0	0.0	2.920644340871906E-5	0.0
54-55	1.3142899533923576E-4	0.0	0.0	2.920644340871906E-5	0.0
56-57	1.7523866045231434E-4	0.0	0.0	2.920644340871906E-5	0.0
58-59	2.920644340871906E-4	0.0	0.0	2.920644340871906E-5	0.0
60-61	3.212708774959096E-4	0.0	0.0	2.920644340871906E-5	0.0
62-63	3.7968376431334776E-4	0.0	0.0	2.920644340871906E-5	0.0
64-65	5.25715981356943E-4	0.0	0.0	2.920644340871906E-5	0.0
66-67	5.695256464700216E-4	0.0	0.0	2.920644340871906E-5	0.0
68-69	6.717481984005383E-4	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
70-71	7.155578635136169E-4	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
72-73	8.177804154441337E-4	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
74-75	9.200029673746503E-4	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
76-77	0.001051431962713886	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
78-79	0.001212067401461841	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
80-81	0.0014749253921403126	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
82-83	0.0016647672742969863	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
84-85	0.001796196269636222	0.0	2.920644340871906E-5	2.920644340871906E-5	0.0
86-87	0.0018107994913405816	5.841288681743812E-5	2.920644340871906E-5	5.841288681743812E-5	0.0
88	0.0018107994913405816	5.841288681743812E-5	2.920644340871906E-5	5.841288681743812E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	12305	0.0	79.7614	8
CACCGGC	12320	0.0	76.15921	9
CTTCACC	14030	0.0	71.7947	6
CCTTCAC	15525	0.0	69.11383	5
GGCGCGC	665	0.0	68.46529	6
GTGTGGC	5040	0.0	68.07806	7
TTCACCG	15240	0.0	66.37166	7
CGTAGTG	2815	0.0	64.86218	4
GCAGCCT	50	1.9989602E-7	63.559204	1
CGGGCCC	945	0.0	61.590004	7
AGGCGCG	755	0.0	60.925552	5
GGGCCCG	965	0.0	60.313522	8
AACGGGC	955	0.0	58.979107	5
CAGCGTA	1505	0.0	58.069042	1
GGCCCGA	995	0.0	58.023296	9
TGTGGCA	6175	0.0	57.389225	8
GCGTAGT	1890	0.0	57.368107	3
CGGGGCA	1405	0.0	57.12683	94
CAGCTGC	130	0.0	57.040314	1
ACCTTCA	6305	0.0	56.28038	4
>>END_MODULE