FastQCFastQC Report
Tue 7 Mar 2017
1SN_12A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_12A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3423902
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG123050.079.76148
CACCGGC123200.076.159219
CTTCACC140300.071.79476
CCTTCAC155250.069.113835
GGCGCGC6650.068.465296
GTGTGGC50400.068.078067
TTCACCG152400.066.371667
CGTAGTG28150.064.862184
GCAGCCT501.9989602E-763.5592041
CGGGCCC9450.061.5900047
AGGCGCG7550.060.9255525
GGGCCCG9650.060.3135228
AACGGGC9550.058.9791075
CAGCGTA15050.058.0690421
GGCCCGA9950.058.0232969
TGTGGCA61750.057.3892258
GCGTAGT18900.057.3681073
CGGGGCA14050.057.1268394
CAGCTGC1300.057.0403141
ACCTTCA63050.056.280384
Produced by FastQC (version 0.11.5)