FastQCFastQC Report
Tue 7 Mar 2017
1SN_13A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_13A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2956991
Sequences flagged as poor quality0
Sequence length94
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA170360.5761262039688319No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG81700.2762943816873301No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA68510.23168822630843314No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT66290.22418059439477497No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA59940.20270606166877073No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG59220.2002711540210978No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG52130.17629407732387417No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG48760.16489735680629397No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT44540.15062609253798878No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42670.14430209628639384No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42390.14335518775674325No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT41920.1417657341534012No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA41760.14122464356502945No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA40960.13851919062317064No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC39670.13415664775442332No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC37500.12681810664963133No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG32420.10963848046882795No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32410.1096046623070547No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32170.10879302642449708No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC30440.1029424844377274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCG257.2907824E-787.999653
GCAGCCA356.0754246E-1087.999653
AGCAGCA500.087.999652
TGCTGCC600.087.999642
AGCTGCA1550.087.999642
GCAGCAT302.0971129E-887.999643
AGCAGCC450.087.999642
CAGCTGC4800.084.332991
AGCAGCG1100.083.999662
CAGCAGC2550.082.82321
CTGCTGC1600.082.4996641
CAGCCCT170000.082.331431
GCTGCAA750.082.1333
CAGCGTA61950.081.323321
AGCCCTT145550.080.895592
GCTGCAT600.080.6663363
AGCTGCC1750.080.456822
AGCTGCT1100.079.999682
GCCCTTC134000.079.3310243
GCTGCTT501.8189894E-1279.1996843
Produced by FastQC (version 0.11.5)