FastQCFastQC Report
Tue 7 Mar 2017
1SN_13A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_13A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2956991
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG111750.080.687528
CACCGGC110550.077.402419
CTTCACC125200.074.3063356
CGCTGCA653.6379788E-1273.3245241
CGGGGCA12500.071.7215994
CCTTCAC136300.071.147735
GTGTGGC50950.069.37097
GGCGCGC4000.069.2338646
CGTAGTG26100.066.360924
AGGCTGC506.1208993E-965.7190552
TTCACCG141850.065.187377
CGGGCCC6900.063.9448177
TGTGGCA56950.062.3095558
AGGCGCG4700.059.9211245
ACCTTCA56050.059.625354
TGTGTGG56200.058.2134066
CAGCGTA13700.057.9816781
GCCTTCA39600.057.8430524
ACGCAGC657.4578566E-1057.7749942
AACGGGC7750.056.3258555
Produced by FastQC (version 0.11.5)