##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_13A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2956991 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.785149160075225 12.0 12.0 12.0 2.0 27.0 2 14.672612124960812 12.0 12.0 12.0 12.0 22.0 3 13.69263957854454 12.0 12.0 12.0 12.0 22.0 4 13.790409575139051 12.0 12.0 12.0 12.0 22.0 5 22.451524877823434 22.0 22.0 22.0 22.0 27.0 6 26.91768929969689 27.0 22.0 32.0 22.0 32.0 7 34.996046318707094 37.0 37.0 37.0 27.0 41.0 8 37.60751419263704 41.0 37.0 41.0 27.0 41.0 9 38.55738756053028 41.0 41.0 41.0 32.0 41.0 10-11 38.91489101590096 41.0 41.0 41.0 37.0 41.0 12-13 39.09101634059759 41.0 41.0 41.0 37.0 41.0 14-15 39.04850437488649 41.0 41.0 41.0 37.0 41.0 16-17 38.962798838413775 41.0 41.0 41.0 37.0 41.0 18-19 38.89371966299525 41.0 41.0 41.0 37.0 41.0 20-21 38.908402156110725 41.0 41.0 41.0 37.0 41.0 22-23 38.8968978938387 41.0 41.0 41.0 37.0 41.0 24-25 38.914646003318914 41.0 41.0 41.0 37.0 41.0 26-27 38.8836869980328 41.0 41.0 41.0 37.0 41.0 28-29 38.8286087106792 41.0 41.0 41.0 37.0 41.0 30-31 38.71033611532805 41.0 41.0 41.0 32.0 41.0 32-33 38.74999213727739 41.0 41.0 41.0 32.0 41.0 34-35 38.7264200330674 41.0 41.0 41.0 32.0 41.0 36-37 38.65610767837981 41.0 41.0 41.0 32.0 41.0 38-39 38.56406495657241 41.0 41.0 41.0 32.0 41.0 40-41 38.60966215318207 41.0 41.0 41.0 32.0 41.0 42-43 38.351060588280454 41.0 41.0 41.0 32.0 41.0 44-45 38.45594338974992 41.0 41.0 41.0 32.0 41.0 46-47 38.47213992196798 41.0 41.0 41.0 32.0 41.0 48-49 38.43617768873831 41.0 41.0 41.0 32.0 41.0 50-51 38.31271924737005 41.0 41.0 41.0 32.0 41.0 52-53 38.28317333397362 41.0 41.0 41.0 32.0 41.0 54-55 38.156497770875866 41.0 39.0 41.0 32.0 41.0 56-57 37.93633984682401 41.0 37.0 41.0 29.5 41.0 58-59 37.9393794232042 41.0 37.0 41.0 29.5 41.0 60-61 37.942109732494956 41.0 37.0 41.0 32.0 41.0 62-63 37.94518836885199 41.0 37.0 41.0 32.0 41.0 64-65 37.91910188431416 41.0 37.0 41.0 32.0 41.0 66-67 37.79975048960243 41.0 37.0 41.0 27.0 41.0 68-69 37.78820733644437 41.0 37.0 41.0 27.0 41.0 70-71 37.76067986003339 41.0 37.0 41.0 27.0 41.0 72-73 37.644780454184676 41.0 37.0 41.0 27.0 41.0 74-75 37.54470710259179 41.0 37.0 41.0 27.0 41.0 76-77 36.49004224226587 39.0 34.5 41.0 24.5 41.0 78-79 37.00615591998758 41.0 37.0 41.0 27.0 41.0 80-81 37.65691762335428 41.0 37.0 41.0 27.0 41.0 82-83 37.85143224987834 41.0 37.0 41.0 32.0 41.0 84-85 37.755191510559214 41.0 37.0 41.0 27.0 41.0 86-87 37.59819610543285 41.0 37.0 41.0 27.0 41.0 88-89 37.5076934627126 41.0 37.0 41.0 27.0 41.0 90-91 37.35560760922167 41.0 37.0 41.0 27.0 41.0 92-93 37.26207299920764 41.0 37.0 41.0 27.0 41.0 94-95 37.10634120969594 41.0 37.0 41.0 27.0 41.0 96-97 37.05378000812313 41.0 37.0 41.0 27.0 41.0 98-99 36.91489203044581 41.0 37.0 41.0 27.0 41.0 100 36.7990156209471 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4276843739934577 1101 2 -0.6531171313736461 1101 3 0.08134261499326811 1101 4 0.21971500912897035 1101 5 -1.0878542826017146 1101 6 -0.7578475357926209 1101 7 -0.17582145462358767 1101 8 0.0054481452255501495 1101 9 0.0063072042937051265 1101 10-11 0.047627831407403676 1101 12-13 0.04690548202424338 1101 14-15 0.11242167632765643 1101 16-17 0.039266882252448454 1101 18-19 0.14580544535144213 1101 20-21 0.10797456373109071 1101 22-23 0.17098894132684705 1101 24-25 0.07632299206957072 1101 26-27 0.1340547042090705 1101 28-29 0.0818708830377659 1101 30-31 -0.06174953641450287 1101 32-33 -0.1535276301099131 1101 34-35 -0.001228865867929585 1101 36-37 0.16231915144075515 1101 38-39 0.048394596593922756 1101 40-41 0.1114465423273785 1101 42-43 0.17678068806397818 1101 44-45 0.05540661271297154 1101 46-47 0.06056404341220656 1101 48-49 0.025891402135790997 1101 50-51 -0.017805043685811484 1101 52-53 -0.05589491796393986 1101 54-55 -0.004696834201148192 1101 56-57 0.23814981340080976 1101 58-59 0.10258831366716947 1101 60-61 0.13106291694589345 1101 62-63 -0.19302562284487834 1101 64-65 -0.05913345386876756 1101 66-67 0.06324516304677985 1101 68-69 -0.07105825441495739 1101 70-71 0.14747637673372083 1101 72-73 0.12283985282708443 1101 74-75 0.09860219975005435 1101 76-77 -0.31439564383524043 1101 78-79 -0.07072417042282098 1101 80-81 0.10183463278954719 1101 82-83 0.121152778043502 1101 84-85 -0.06376149899961803 1101 86-87 -0.18635291827470724 1101 88-89 -0.39597656393701186 1101 90-91 -0.25008097523345185 1101 92-93 0.022652534257623813 1101 94-95 -0.017452808101985795 1101 96-97 -0.10237397892675659 1101 98-99 -0.260728368586868 1101 100 -0.5204383861757549 1102 1 1.6107337685672682 1102 2 0.7454726910927043 1102 3 -0.02283981990147055 1102 4 0.09570628662470249 1102 5 -0.8068626141276951 1102 6 -0.09746293118969973 1102 7 0.04159788212970028 1102 8 -0.18843813468244264 1102 9 -0.3537719946286728 1102 10-11 -0.2772757887564552 1102 12-13 -0.3756422357691065 1102 14-15 -0.3135305033394076 1102 16-17 -0.32404504317545246 1102 18-19 -0.3031296183190051 1102 20-21 -0.28609134488031884 1102 22-23 -0.29101903463020307 1102 24-25 -0.2572890423896368 1102 26-27 -0.270741935443219 1102 28-29 -0.3317879802924537 1102 30-31 -0.34929540259370384 1102 32-33 -0.48260918522973384 1102 34-35 -0.22352089861567492 1102 36-37 -0.39180057938914103 1102 38-39 -0.4378065993570175 1102 40-41 -0.35600326122447257 1102 42-43 -0.33154943629662625 1102 44-45 -0.20045422557397785 1102 46-47 -0.35458466849875947 1102 48-49 -0.345689714055581 1102 50-51 -0.35516006819941737 1102 52-53 -0.28331658583304886 1102 54-55 -0.26535954977403264 1102 56-57 -0.1731645818295391 1102 58-59 -0.3157776331568414 1102 60-61 -0.3731628944078267 1102 62-63 -0.4414076366633708 1102 64-65 -0.5211769867190554 1102 66-67 -0.4057779961462913 1102 68-69 -0.4359498796600576 1102 70-71 -0.6414570197462908 1102 72-73 -0.4474170272842244 1102 74-75 -0.6511281727711022 1102 76-77 -0.16998766610772265 1102 78-79 -0.33840516755024197 1102 80-81 -0.33070265399818766 1102 82-83 -0.445402054079274 1102 84-85 -0.4441959865303531 1102 86-87 -0.36475719572470666 1102 88-89 -0.4072900739335168 1102 90-91 -0.36145999253535166 1102 92-93 -0.304928328166703 1102 94-95 -0.36589855875816113 1102 96-97 -0.33417843962016036 1102 98-99 -0.484354374987106 1102 100 -0.6821160223652996 1103 1 1.624452572559628 1103 2 0.6285584503182804 1103 3 0.3781937969376177 1103 4 1.0512985666850678 1103 5 2.788804636554751 1103 6 0.02519343979493982 1103 7 -0.5274470546963883 1103 8 -0.4487873599252836 1103 9 -0.40248771659667426 1103 10-11 -0.40223547952161454 1103 12-13 -0.5006108841420058 1103 14-15 -0.4096241513327499 1103 16-17 -0.6058754468143661 1103 18-19 -0.5678893266957346 1103 20-21 -0.4280618363948818 1103 22-23 -0.3900711982720324 1103 24-25 -0.39317238397206467 1103 26-27 -0.45966532208696975 1103 28-29 -0.38206801940228274 1103 30-31 -0.29295523819882163 1103 32-33 -0.6978179126725053 1103 34-35 -0.6453614440884436 1103 36-37 -0.44788454102020836 1103 38-39 -0.3647373433850234 1103 40-41 -0.46968198371778414 1103 42-43 -0.3067619897006608 1103 44-45 -0.7111948527279779 1103 46-47 -0.8180932723569114 1103 48-49 -0.5854015220044744 1103 50-51 -0.5879092339272987 1103 52-53 -0.48888943243933625 1103 54-55 -0.3883806016704625 1103 56-57 -0.2850038064347302 1103 58-59 -0.6244336428006889 1103 60-61 -0.4632649655889196 1103 62-63 -0.40304721289261636 1103 64-65 -0.5099831653428666 1103 66-67 -0.6064368357869014 1103 68-69 -0.5174827844452423 1103 70-71 -0.7001632071758834 1103 72-73 -0.6211664541491544 1103 74-75 -0.6010077005381689 1103 76-77 -0.6167546529202994 1103 78-79 -0.8550298043116484 1103 80-81 -0.5430703015481981 1103 82-83 -0.5136935649999614 1103 84-85 -0.5925473312342859 1103 86-87 -0.49143538076155124 1103 88-89 -0.39597297158297096 1103 90-91 -0.761199310967676 1103 92-93 -0.5393934071606594 1103 94-95 -0.5374545863172315 1103 96-97 -0.4549756515267944 1103 98-99 -0.5109820785904944 1103 100 -0.32258649414276164 1104 1 1.871144225038039 1104 2 -0.4952646081429677 1104 3 -0.010699181172446615 1104 4 0.01803801478500766 1104 5 -1.135039719374813 1104 6 -0.5573273116617941 1104 7 -0.29986761755287716 1104 8 -0.007493258177682094 1104 9 -0.06154179527301551 1104 10-11 -0.22187397762173333 1104 12-13 -0.1668460709383055 1104 14-15 -0.1402813139790382 1104 16-17 -0.0932530768707096 1104 18-19 0.014488134428845001 1104 20-21 0.04089303107211606 1104 22-23 -0.10016227662379151 1104 24-25 -0.22572888259587387 1104 26-27 -0.16510172712258253 1104 28-29 -0.14008066394451646 1104 30-31 -0.22447905094038134 1104 32-33 -0.19308676756857324 1104 34-35 -0.17781338601935204 1104 36-37 -0.2185260412790342 1104 38-39 -0.187287953530344 1104 40-41 -0.08728473344417864 1104 42-43 -0.1775534772292815 1104 44-45 -0.08071015130882842 1104 46-47 -0.3866978175865725 1104 48-49 -0.4735554236644717 1104 50-51 -0.30347671794213227 1104 52-53 -0.24280623596084183 1104 54-55 -0.15781982062654265 1104 56-57 -0.25352970789683127 1104 58-59 -0.19475073374751162 1104 60-61 -0.19362468767204177 1104 62-63 -0.3491254017510883 1104 64-65 -0.4023844941118213 1104 66-67 -0.7700223580937333 1104 68-69 -0.7301811435145211 1104 70-71 -0.5779516370396394 1104 72-73 -0.3953715095527386 1104 74-75 -0.5342016752249563 1104 76-77 -0.47708866117623927 1104 78-79 -0.30778306016966184 1104 80-81 -0.3427940076214213 1104 82-83 -0.48955608230038195 1104 84-85 -0.4377717510441883 1104 86-87 -0.32844217543826915 1104 88-89 -0.37433944972983824 1104 90-91 -0.35319079922027896 1104 92-93 -0.4234329154727092 1104 94-95 -0.3062550320557449 1104 96-97 -0.49742654366748695 1104 98-99 -0.4599198899830128 1104 100 -0.5385090365174818 1105 1 1.6500023667210932 1105 2 0.2718226987133132 1105 3 0.07225361172177891 1105 4 -0.2466188755985712 1105 5 -1.1684808046341075 1105 6 0.29670579279622444 1105 7 0.12373774940591886 1105 8 0.06441974130998318 1105 9 -0.06977358207166873 1105 10-11 -0.0798232813548907 1105 12-13 -0.001706857068967338 1105 14-15 0.04071756334497678 1105 16-17 -0.06885864747362547 1105 18-19 -0.01947932406038433 1105 20-21 -0.051375821881904926 1105 22-23 -0.032804790200799516 1105 24-25 0.018837169637087925 1105 26-27 0.024557657608845318 1105 28-29 -0.025331446116410916 1105 30-31 0.0295068584081406 1105 32-33 -0.058749092265657055 1105 34-35 -0.06903692435345476 1105 36-37 -0.20012287449596755 1105 38-39 -0.21390593676891 1105 40-41 0.0021582647824232026 1105 42-43 0.10916339629514482 1105 44-45 0.014264227861886525 1105 46-47 -0.18198683569715257 1105 48-49 -0.22396007857107492 1105 50-51 -0.10632638966840346 1105 52-53 -0.1534069802566762 1105 54-55 0.07894667652200837 1105 56-57 -0.020286605097808774 1105 58-59 -0.12950378745836133 1105 60-61 -0.20293621255393646 1105 62-63 -0.0691988599295783 1105 64-65 -0.0958065404016537 1105 66-67 -0.04556126158134788 1105 68-69 -0.1356245127479383 1105 70-71 -0.3849214447510221 1105 72-73 -0.23970430734494386 1105 74-75 -0.29033425983455174 1105 76-77 -0.3358719256090055 1105 78-79 -0.13683311302643375 1105 80-81 -0.35944023185173535 1105 82-83 -0.134979268529122 1105 84-85 -0.16975934104186763 1105 86-87 -0.11576705539503962 1105 88-89 -0.2635345931687425 1105 90-91 -0.27846013758463783 1105 92-93 -0.16213864006686407 1105 94-95 -0.01840794830680892 1105 96-97 -0.17879891483698174 1105 98-99 -0.3482049256221984 1105 100 -0.1780307127660521 1106 1 1.0539721813358973 1106 2 -0.6252637881821723 1106 3 -0.11113405534385912 1106 4 -0.10177801904692885 1106 5 -1.1148002554286052 1106 6 -0.6583836453280298 1106 7 -0.41949547333180703 1106 8 -0.10128202565862665 1106 9 -0.26991477479228365 1106 10-11 -0.22485680622691717 1106 12-13 -0.1732576924373248 1106 14-15 0.04364710049403442 1106 16-17 -0.11225021172712246 1106 18-19 -0.050031129635698335 1106 20-21 -0.14188611101292992 1106 22-23 -0.19291296783894296 1106 24-25 -0.2686314140331163 1106 26-27 -0.25601591262130796 1106 28-29 -0.3190171983890622 1106 30-31 -0.15155681094906726 1106 32-33 -0.10895598795102757 1106 34-35 -0.25406015410553806 1106 36-37 -0.484943839029512 1106 38-39 -0.22009995303718455 1106 40-41 -0.25059452584071806 1106 42-43 -0.13675806806589463 1106 44-45 -0.211377982831074 1106 46-47 -0.4275097439458406 1106 48-49 -0.3662607515809455 1106 50-51 -0.33325176553230307 1106 52-53 -0.546598908578801 1106 54-55 -0.383837589589902 1106 56-57 -0.15827385948222172 1106 58-59 -0.2346492001848688 1106 60-61 -0.24659238534600547 1106 62-63 -0.2844060141615117 1106 64-65 -0.5965898488687458 1106 66-67 -0.5778253173853329 1106 68-69 -0.5027275583096795 1106 70-71 -0.7277158540685136 1106 72-73 -0.539673106470623 1106 74-75 -0.6478078333075032 1106 76-77 -0.6135367738801492 1106 78-79 -0.4032497977816192 1106 80-81 -0.5007896871948958 1106 82-83 -0.6466720737185199 1106 84-85 -0.7559870326358364 1106 86-87 -0.6709549895378188 1106 88-89 -0.6182176165334639 1106 90-91 -0.7545834347925435 1106 92-93 -0.4982114228255128 1106 94-95 -0.37868485314502465 1106 96-97 -0.5642854980228122 1106 98-99 -0.820757943006754 1106 100 -0.8773899846989082 1107 1 1.2593666899192666 1107 2 -0.7675424221748433 1107 3 -0.19748657595825136 1107 4 -0.2609061777230668 1107 5 -1.2059627026435784 1107 6 -0.42403408812508303 1107 7 -0.24627544180565053 1107 8 -0.1235871364199781 1107 9 -0.1312528981463572 1107 10-11 -0.11510977229905706 1107 12-13 -0.0701345807843552 1107 14-15 -0.2596866191312941 1107 16-17 -0.15247503366819615 1107 18-19 -0.1746660245741296 1107 20-21 -0.19977196645852757 1107 22-23 -0.10408660175475148 1107 24-25 -0.21524891518902933 1107 26-27 -0.22735767815704833 1107 28-29 -0.027325304490076974 1107 30-31 -0.32195710799972943 1107 32-33 -0.22876366183859176 1107 34-35 -0.1308955830729417 1107 36-37 -0.0811311370392005 1107 38-39 -0.1376877585872549 1107 40-41 -0.11843728410746479 1107 42-43 -0.04406705751421214 1107 44-45 -0.10101810453670623 1107 46-47 -0.22643825667041284 1107 48-49 -0.2644509834389268 1107 50-51 -0.2148246913512324 1107 52-53 -0.23480332484233202 1107 54-55 -0.26586933569673477 1107 56-57 -0.14696388025920726 1107 58-59 -0.25100531567986195 1107 60-61 -0.0739980581622035 1107 62-63 -0.1330191175013269 1107 64-65 -0.16176479775403863 1107 66-67 -0.21485988973052628 1107 68-69 -0.520094881790996 1107 70-71 -0.2679830991483172 1107 72-73 -0.24973401249878435 1107 74-75 -0.22946882778864364 1107 76-77 -0.3366319306062948 1107 78-79 -0.5735074525713841 1107 80-81 -0.48190991230065094 1107 82-83 -0.3789372801226065 1107 84-85 -0.33996607471840434 1107 86-87 -0.32511683248173995 1107 88-89 -0.26635803446198736 1107 90-91 -0.11261151861475582 1107 92-93 -0.22965048664291743 1107 94-95 -0.1377734563092261 1107 96-97 -0.12903222449378404 1107 98-99 -0.38874656591914913 1107 100 -0.403422123404539 1108 1 1.505019470030735 1108 2 -0.5435587099493606 1108 3 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0.19309180873577958 2223 18-19 0.13632037957698628 2223 20-21 0.19368406318832854 2223 22-23 0.22135579505400216 2223 24-25 0.3326492303797224 2223 26-27 0.21008286456723368 2223 28-29 0.09287592262718647 2223 30-31 0.21234229890130507 2223 32-33 0.14953025678744325 2223 34-35 0.1313819470183617 2223 36-37 0.17961480830692267 2223 38-39 0.14968536551040756 2223 40-41 0.1252564879487963 2223 42-43 0.20917022137408026 2223 44-45 0.26519161044982553 2223 46-47 0.2820215817098273 2223 48-49 0.3367441439253511 2223 50-51 0.2694788768366507 2223 52-53 0.3733080635594703 2223 54-55 0.29134113410099616 2223 56-57 0.3482286775104342 2223 58-59 0.4486827033973242 2223 60-61 0.41213633175268427 2223 62-63 0.3586913967990526 2223 64-65 0.3260151788271628 2223 66-67 0.32181853064497545 2223 68-69 0.5812984846553988 2223 70-71 0.7324126565702684 2223 72-73 0.45972999641561074 2223 74-75 0.5412212927384061 2223 76-77 0.4616433315836659 2223 78-79 0.5490882483569308 2223 80-81 0.4112240510823426 2223 82-83 0.3487378493685469 2223 84-85 0.4193908966507891 2223 86-87 0.45291030142037414 2223 88-89 0.4676545326243158 2223 90-91 0.6156886708393898 2223 92-93 0.5642022523507606 2223 94-95 0.5812041501879364 2223 96-97 0.6836496150724258 2223 98-99 0.5055701854566763 2223 100 0.7829538234327131 2224 1 1.1830904633737553 2224 2 -0.985057506488598 2224 3 -0.148225311323948 2224 4 -0.39397718560002915 2224 5 -0.9966925098235677 2224 6 0.476060089833819 2224 7 0.23875256677693102 2224 8 0.15847052237932502 2224 9 0.2065461600740548 2224 10-11 0.257897298248011 2224 12-13 0.1439264476850397 2224 14-15 0.1254358554161783 2224 16-17 0.10656216230547955 2224 18-19 0.0107950730166948 2224 20-21 0.10397355144824871 2224 22-23 0.12972501352684418 2224 24-25 0.21345880193148759 2224 26-27 0.24767090643751288 2224 28-29 0.16001013608709513 2224 30-31 0.04286497629962582 2224 32-33 0.2846114803643687 2224 34-35 0.19391253293586885 2224 36-37 0.052101154192456534 2224 38-39 0.11980651729368219 2224 40-41 0.23699299684237474 2224 42-43 0.3349546581374767 2224 44-45 0.42031910727730093 2224 46-47 0.30620131823274477 2224 48-49 0.1522411957584069 2224 50-51 0.26896762752900116 2224 52-53 0.2307068057683921 2224 54-55 0.3726317371255874 2224 56-57 0.5575246931031188 2224 58-59 0.3154170739058344 2224 60-61 0.3746492497017968 2224 62-63 0.2747144913577344 2224 64-65 0.1771259471734581 2224 66-67 0.2739197568861158 2224 68-69 0.4215305520180337 2224 70-71 0.4348198471529585 2224 72-73 0.35447454703925274 2224 74-75 0.2523209134379698 2224 76-77 0.3309378748456524 2224 78-79 0.25339439498704763 2224 80-81 0.3560581334347006 2224 82-83 0.26171195738152875 2224 84-85 0.0978694882613027 2224 86-87 0.2162818686112402 2224 88-89 0.29763300855170627 2224 90-91 0.265488433864256 2224 92-93 0.21374337594942006 2224 94-95 0.5678193320207754 2224 96-97 0.35256282468323974 2224 98-99 0.39086426598615276 2224 100 0.5913943447779957 2225 1 1.5712985917786852 2225 2 1.953261662805506 2225 3 0.8447033788878606 2225 4 0.9657310363687657 2225 5 3.166384717437765 2225 6 -0.03039835898321641 2225 7 0.34843625029803604 2225 8 0.439370288999271 2225 9 0.2305908730095254 2225 10-11 0.26010597038257544 2225 12-13 0.26129041818241205 2225 14-15 0.3888351542167925 2225 16-17 0.36445432730270966 2225 18-19 0.22116795150019186 2225 20-21 0.2883420306647082 2225 22-23 0.3612682523900972 2225 24-25 0.3447112513628596 2225 26-27 0.2964106894399592 2225 28-29 0.3041592874804877 2225 30-31 0.3091052977356341 2225 32-33 0.294306179706588 2225 34-35 0.2816729670721969 2225 36-37 0.2616104205458285 2225 38-39 0.2779686405225874 2225 40-41 0.3112948515253322 2225 42-43 0.6402664729587002 2225 44-45 0.3225372417197505 2225 46-47 0.339405827657977 2225 48-49 0.3739083273021393 2225 50-51 0.3175829882441761 2225 52-53 0.2566018941950645 2225 54-55 0.2513128043891726 2225 56-57 0.37167366796091983 2225 58-59 0.6021992890038632 2225 60-61 0.5203418871082732 2225 62-63 0.4741313589807561 2225 64-65 0.48215820022664246 2225 66-67 0.4955563124648208 2225 68-69 0.47934680670946506 2225 70-71 0.44383248357085137 2225 72-73 0.474929938259784 2225 74-75 0.5193878451434131 2225 76-77 0.6500580920550547 2225 78-79 0.4559698374735177 2225 80-81 0.3980774181830924 2225 82-83 0.36282469654312877 2225 84-85 0.39098664787419324 2225 86-87 0.35148059473038984 2225 88-89 0.3824991769172357 2225 90-91 0.4734701024080863 2225 92-93 0.39525091931538725 2225 94-95 0.5867988856681805 2225 96-97 0.4930768963355092 2225 98-99 0.5685105522068454 2225 100 0.7215430230560003 2226 1 1.3413082256831732 2226 2 1.5673584431960492 2226 3 0.8201579899145024 2226 4 0.8052454704429355 2226 5 3.144543820406277 2226 6 -0.31102964039249414 2226 7 0.30861458822455745 2226 8 0.4329810606900182 2226 9 0.39641100822697695 2226 10-11 0.18538029384895083 2226 12-13 0.2617481179145358 2226 14-15 0.20467617836725793 2226 16-17 0.171323394042318 2226 18-19 0.12087757747759298 2226 20-21 0.24199273771046137 2226 22-23 0.2471007709955373 2226 24-25 0.2749732092950481 2226 26-27 0.0823782541045972 2226 28-29 0.24907149325897393 2226 30-31 0.2193319480149043 2226 32-33 0.1480310870989925 2226 34-35 0.18470454042663675 2226 36-37 0.28631842182844025 2226 38-39 0.23134654167401436 2226 40-41 0.2860925145357882 2226 42-43 0.5869504887471635 2226 44-45 0.3274773851071018 2226 46-47 0.22187469243552016 2226 48-49 0.38870604744471393 2226 50-51 0.3010570927516838 2226 52-53 0.4152373076852456 2226 54-55 0.3949118282868369 2226 56-57 0.4560336060582628 2226 58-59 0.3776554711639335 2226 60-61 0.43809041006431926 2226 62-63 0.43209625557518194 2226 64-65 0.393431796686734 2226 66-67 0.5078808543544469 2226 68-69 0.4116897308601395 2226 70-71 0.4347346926558586 2226 72-73 0.4120813876660705 2226 74-75 0.46178330942404955 2226 76-77 0.5565972921910216 2226 78-79 0.6011194272291576 2226 80-81 0.4213666859849212 2226 82-83 0.3945550929043833 2226 84-85 0.22361123339849343 2226 86-87 0.4894405351130118 2226 88-89 0.43081181532873103 2226 90-91 0.3448001311241242 2226 92-93 0.34036387309621574 2226 94-95 0.3984859044639322 2226 96-97 0.4571972080198137 2226 98-99 0.45659329094949896 2226 100 0.7227082327344689 2227 1 1.275333210012441 2227 2 2.0193333784859746 2227 3 0.5290313964883833 2227 4 0.06540782557239488 2227 5 3.247352988612988 2227 6 0.010476526719205737 2227 7 0.2710699821645477 2227 8 0.36488004316770883 2227 9 0.23428263725686094 2227 10-11 0.2310272853151858 2227 12-13 0.23123429417836405 2227 14-15 0.2691814239200454 2227 16-17 0.30482449293256764 2227 18-19 0.17232672357253165 2227 20-21 0.2307500715777877 2227 22-23 0.21022043474992103 2227 24-25 0.26722936459641033 2227 26-27 0.36913297771096154 2227 28-29 0.30379474707406473 2227 30-31 0.26496455554715226 2227 32-33 0.1970512521052754 2227 34-35 0.2962888955010996 2227 36-37 0.1851481759830449 2227 38-39 0.23520947406458959 2227 40-41 0.21663729193382864 2227 42-43 0.3979513778717063 2227 44-45 0.20387497114452913 2227 46-47 0.33074767595252297 2227 48-49 0.331245333564425 2227 50-51 0.44573923248878344 2227 52-53 0.41827986227192326 2227 54-55 0.3089666240243929 2227 56-57 0.46464471736523194 2227 58-59 0.5032111387512188 2227 60-61 0.34519741313641816 2227 62-63 0.4061001857736173 2227 64-65 0.3660010704884442 2227 66-67 0.42331721285003 2227 68-69 0.32350958759896997 2227 70-71 0.5132402301178942 2227 72-73 0.2018919216414119 2227 74-75 0.27775709117887715 2227 76-77 0.6651512908189119 2227 78-79 0.5346153789273416 2227 80-81 0.3245220557401609 2227 82-83 0.3259887128117498 2227 84-85 0.25485666350279956 2227 86-87 0.2998697333708691 2227 88-89 0.2756816513025271 2227 90-91 0.34409496581111654 2227 92-93 0.1695873733419404 2227 94-95 0.5787769534969271 2227 96-97 0.39099938239117193 2227 98-99 0.6104201577408759 2227 100 0.5815230106754328 2228 1 1.375776665974989 2228 2 1.824417027439491 2228 3 -0.3202296815092627 2228 4 -0.08324726722785414 2228 5 3.1399509373340706 2228 6 0.2697728684505307 2228 7 0.272078013363938 2228 8 0.42523790743580037 2228 9 0.33145162350158586 2228 10-11 0.2923708927254367 2228 12-13 0.2533391866360546 2228 14-15 0.23021995573558485 2228 16-17 0.28811803178447093 2228 18-19 0.10686295006696866 2228 20-21 0.19585530592455314 2228 22-23 0.2619470579106675 2228 24-25 0.30167843853805465 2228 26-27 0.21636896699380515 2228 28-29 0.28434942391389484 2228 30-31 0.33369016192811785 2228 32-33 0.34363737440824593 2228 34-35 0.2850754336811647 2228 36-37 0.3587336758868105 2228 38-39 0.36905252114890885 2228 40-41 0.39380547553076184 2228 42-43 0.508808980654365 2228 44-45 0.39902635357343286 2228 46-47 0.3238481682894587 2228 48-49 0.40984302177214005 2228 50-51 0.3223724055054902 2228 52-53 0.3726599584952339 2228 54-55 0.3667340050290804 2228 56-57 0.5018580658701808 2228 58-59 0.6060744806044696 2228 60-61 0.33719801415020356 2228 62-63 0.5007752703363124 2228 64-65 0.4508983635101558 2228 66-67 0.46254474905736487 2228 68-69 0.40389365317946613 2228 70-71 0.6815433215894657 2228 72-73 0.5906488951648683 2228 74-75 0.5675441895460054 2228 76-77 0.7037529561603364 2228 78-79 0.6979828808013409 2228 80-81 0.6080728723718067 2228 82-83 0.4775586807863803 2228 84-85 0.5545404230495095 2228 86-87 0.6139047104842916 2228 88-89 0.7433163371297979 2228 90-91 0.6616590241872231 2228 92-93 0.5708602366532389 2228 94-95 0.9406569601514079 2228 96-97 0.5919542694191904 2228 98-99 0.738247165200498 2228 100 0.7754899321434294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 20.0 13 41.0 14 160.0 15 488.0 16 1829.0 17 4682.0 18 7588.0 19 9797.0 20 11686.0 21 13556.0 22 15603.0 23 17854.0 24 20328.0 25 23281.0 26 26114.0 27 29537.0 28 33163.0 29 38269.0 30 43774.0 31 50212.0 32 59377.0 33 70871.0 34 87377.0 35 112576.0 36 158383.0 37 260366.0 38 637238.0 39 1221256.0 40 1565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.44155879321056 19.93832497308487 19.09927459764596 35.520841636058606 2 18.387737066902776 50.96713928138168 14.080362877192437 16.56476077452311 3 41.07854910616908 16.24506128020004 23.285190925505013 19.39119868812587 4 16.878407813889186 15.830585889507272 23.141903374071816 44.149102922531725 5 17.6325866395941 35.64826541575541 26.390746539302963 20.328401405347527 6 17.622069191282623 27.708505030958836 31.490491516545028 23.17893426121351 7 20.63969758447016 26.972452739964375 35.84332857286343 16.544521102702035 8 20.738649525818644 27.808268608189877 34.210722995098735 17.242358870892744 9 20.874767626955915 28.191360744757088 27.84749767584683 23.086373952440166 10-11 19.538578237133628 30.27028151252405 28.839113815361628 21.352026434980694 12-13 24.017337218814667 26.87825225034503 27.432988466992292 21.671422063848013 14-15 21.58239575297997 27.501368790097775 28.739468601696792 22.176766855225463 16-17 20.797560763627622 29.59946445559016 28.43422587353157 21.16874890725065 18-19 21.3168893648983 29.43069491926083 29.84975267087387 19.402663044966996 20-21 22.70454999693946 28.854281260916924 30.06936781342926 18.371800928714357 22-23 21.753346560743676 30.257802610829724 28.81571164741455 19.17313918101205 24-25 22.033479303792266 28.392325171094534 26.779249581753884 22.794945943359313 26-27 20.130210068275485 26.747646509576796 29.97611423233956 23.146029189808154 28-29 19.875748250622554 27.960481984916708 30.700540635962938 21.463229128497805 30-31 20.18161814294189 29.996232283445924 28.344558693515538 21.47759088009665 32-33 20.66220357113025 30.220399723908525 28.757713499973452 20.35968320498777 34-35 21.258451581354155 29.266558471094434 30.00014203627945 19.474847911271965 36-37 21.69293041473579 28.055175007296267 28.96997319234316 21.281921385624777 38-39 21.389918144124444 29.31173867263585 28.9729961340435 20.32534704919621 40-41 20.81918442065635 29.53969998048659 30.09172316931586 19.549392429541204 42-43 20.64107769067154 29.942886168517003 29.185663324134225 20.23037281667723 44-45 20.486907129578682 30.076689445453162 29.02582388651166 20.410579538456492 46-47 19.797763562607905 28.213301949484855 29.048846187014593 22.940088300892647 48-49 20.845531826102953 28.1060544316841 30.335195474047772 20.713218268165175 50-51 21.85165595701847 30.65405001232672 28.28348141742738 19.210812613227432 52-53 20.234816240582994 30.279844048792835 28.515695477486013 20.96964423313816 54-55 22.219324579860178 28.37520229236208 28.386347504326594 21.01912562345115 56-57 21.392741870294728 28.631085716063787 29.513501227907057 20.46267118573443 58-59 21.014539565761197 28.092498804378778 29.198000285460907 21.694961344399115 60-61 19.71861874825519 30.28647935083867 29.52849431574633 20.466407585159814 62-63 20.49031399198278 31.08595376637503 27.656972315834615 20.766759925807573 64-65 19.632416685957118 30.262112868510354 29.06510151625496 21.040368929277562 66-67 20.17178949817568 30.10702433656376 29.62626534879545 20.094920816465116 68-69 20.764080783472117 30.113027060278508 29.266998107197484 19.85589404905189 70-71 19.79765917447838 30.706011617891292 29.35957532505172 20.13675388257861 72-73 20.01352050107694 30.693667988844066 28.672813004841746 20.61999850523725 74-75 19.988038516180808 29.272679558375387 30.54052244325397 20.198759482189836 76-77 19.51191261657543 30.465615214926256 28.819330190724283 21.203141977774028 78-79 19.62978581943604 29.40095184598127 28.467519853797324 22.501742480785367 80-81 20.552690872044607 29.671346260340968 28.537219044720345 21.238743822894076 82-83 20.312439232990563 28.14981513301867 30.023493476983866 21.5142521570069 84-85 20.702058274780004 28.899377779641533 28.467164763098708 21.93139918247976 86-87 21.403666768008424 29.069094224500514 28.85945543966823 20.66778356782283 88-89 20.602024152254774 29.128495825655204 29.003994263087034 21.265485759002985 90-91 20.323616135456618 29.804554697663942 28.702116442018255 21.169712724861185 92-93 20.211272202045933 29.831051227413276 30.2760306000255 19.681645970515298 94-95 20.17672694979457 30.094951252810713 29.620566988536655 20.107754808858058 96-97 20.12190770956016 29.942668070345835 29.50151015001398 20.43391407008002 98-99 20.94612394829744 30.55142880042584 28.871782159634574 19.630665091642147 100 20.614942690052153 31.319337799810686 27.735999196480478 20.329720313656686 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 10.0 2 19.5 3 25.5 4 27.5 5 33.5 6 49.0 7 58.5 8 59.0 9 62.0 10 92.5 11 107.5 12 102.5 13 145.0 14 185.0 15 226.0 16 290.0 17 387.0 18 552.5 19 883.0 20 1423.0 21 2180.0 22 3264.5 23 5016.0 24 7730.0 25 11671.5 26 16751.5 27 22433.0 28 28797.5 29 36467.5 30 45474.0 31 55304.0 32 66592.0 33 78861.0 34 91706.0 35 106399.5 36 121059.5 37 131475.0 38 137896.5 39 142395.5 40 144965.0 41 144451.0 42 141105.0 43 137822.5 44 136608.5 45 133899.0 46 123579.0 47 110981.5 48 103223.0 49 98963.0 50 94844.5 51 86716.5 52 74787.0 53 62991.0 54 52901.5 55 43934.0 56 35094.0 57 26123.5 58 18340.0 59 12921.0 60 9833.0 61 8161.0 62 6706.5 63 5109.0 64 3758.5 65 3229.5 66 3606.0 67 3406.5 68 2348.5 69 2090.5 70 2564.5 71 2362.0 72 1360.5 73 571.0 74 284.0 75 172.0 76 103.5 77 84.5 78 75.5 79 56.0 80 43.5 81 30.5 82 23.5 83 20.5 84 25.0 85 24.5 86 16.5 87 19.0 88 20.0 89 18.0 90 17.0 91 15.0 92 9.5 93 9.0 94 12.0 95 16.5 96 17.5 97 17.5 98 44.0 99 105.5 100 105.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.324349651385479 2 0.006019632795635834 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.550906986189677E-4 30-31 0.009908721399557861 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.623631252174931E-4 40-41 0.001690908088661751 42-43 5.0727242659852536E-5 44-45 0.0 46-47 0.001893817059301161 48-49 0.0 50-51 0.0 52-53 0.0013696355518160183 54-55 0.01909035232099117 56-57 0.011295266032260496 58-59 0.012478901694323722 60-61 2.1981805152602763E-4 62-63 0.012005447429498432 64-65 5.0727242659852536E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1836356620632257E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2956991.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.62836347722389 #Duplication Level Percentage of deduplicated Percentage of total 1 86.00085553301892 63.32102250537339 2 8.60398981870886 12.669953794524591 3 2.4459042541955545 5.402637823751955 4 1.0397026525514173 3.062064192411583 5 0.5450004889200389 2.006374704673468 6 0.33948383443438856 1.4997383493826117 7 0.20744179982747513 1.0691520166646802 8 0.1482319356740982 0.8731259870995979 9 0.1179082163499048 0.7813250109325749 >10 0.5166487496136996 6.431447755451323 >50 0.023620685165575157 1.1866711004899189 >100 0.010697619815955968 1.410149541167925 >500 4.676470219789804E-4 0.24842749260979535 >1k 4.676470216951696E-5 0.03790972546654688 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.381816177323502E-5 0.0 2 0.0 0.0 0.0 3.381816177323502E-5 0.0 3 0.0 0.0 0.0 3.381816177323502E-5 0.0 4 0.0 0.0 0.0 3.381816177323502E-5 0.0 5 0.0 0.0 0.0 3.381816177323502E-5 0.0 6 0.0 0.0 0.0 3.381816177323502E-5 0.0 7 0.0 0.0 0.0 3.381816177323502E-5 0.0 8 0.0 0.0 0.0 3.381816177323502E-5 0.0 9 0.0 0.0 0.0 3.381816177323502E-5 0.0 10-11 0.0 0.0 0.0 3.381816177323502E-5 0.0 12-13 0.0 0.0 0.0 3.381816177323502E-5 0.0 14-15 0.0 0.0 0.0 1.3527264709294008E-4 0.0 16-17 1.690908088661751E-5 0.0 0.0 1.3527264709294008E-4 0.0 18-19 3.381816177323502E-5 0.0 0.0 1.3527264709294008E-4 0.0 20-21 3.381816177323502E-5 0.0 0.0 1.3527264709294008E-4 0.0 22-23 3.381816177323502E-5 1.690908088661751E-5 1.690908088661751E-5 1.3527264709294008E-4 0.0 24-25 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 26-27 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 28-29 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 30-31 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 32-33 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 34-35 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 36-37 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 38-39 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 40-41 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 42-43 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 44-45 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 46-47 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 48-49 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 50-51 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 52-53 3.381816177323502E-5 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 54-55 1.1836356620632257E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 56-57 1.690908088661751E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 58-59 2.5363621329926265E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 60-61 2.7054529418588016E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 62-63 3.381816177323502E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 64-65 4.0581794127882024E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 66-67 4.565451839386728E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 68-69 5.749087501449953E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 70-71 6.594541545780828E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 72-73 7.439995590111704E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 74-75 7.778177207844055E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 76-77 9.469085296505807E-4 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 78-79 0.0010821811767435206 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 80-81 0.0014710900371357233 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 82-83 0.0016401808460018985 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 84-85 0.0017078171695483685 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 86-87 0.0018599988975279262 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 88 0.0018599988975279262 3.381816177323502E-5 3.381816177323502E-5 1.3527264709294008E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11175 0.0 80.68752 8 CACCGGC 11055 0.0 77.40241 9 CTTCACC 12520 0.0 74.306335 6 CGCTGCA 65 3.6379788E-12 73.324524 1 CGGGGCA 1250 0.0 71.72159 94 CCTTCAC 13630 0.0 71.14773 5 GTGTGGC 5095 0.0 69.3709 7 GGCGCGC 400 0.0 69.233864 6 CGTAGTG 2610 0.0 66.36092 4 AGGCTGC 50 6.1208993E-9 65.719055 2 TTCACCG 14185 0.0 65.18737 7 CGGGCCC 690 0.0 63.944817 7 TGTGGCA 5695 0.0 62.309555 8 AGGCGCG 470 0.0 59.921124 5 ACCTTCA 5605 0.0 59.62535 4 TGTGTGG 5620 0.0 58.213406 6 CAGCGTA 1370 0.0 57.981678 1 GCCTTCA 3960 0.0 57.843052 4 ACGCAGC 65 7.4578566E-10 57.774994 2 AACGGGC 775 0.0 56.325855 5 >>END_MODULE