FastQCFastQC Report
Tue 7 Mar 2017
1SN_14A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_14A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3544290
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA184610.5208659562281868No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG99760.28146680999579604No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA75980.2143729773805191No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG73480.20731937849329485No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT72480.20449793893840518No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG68850.19425611335415557No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA68070.1920553905013416No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG66120.1865535833693067No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC65520.18486071963637285No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG60540.17080995065302218No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA59450.1677345815381924No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT53650.1513702321198322No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG52520.14818200542280682No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT51440.14513485070352597No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC51210.14448591960590132No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA51080.14411913246376568No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC49890.14076161939344692No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA47330.1335387341329293No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA46510.13122515369791976No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA42510.11993939547836098No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG39940.11268829582229443No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA39470.11136221923149629No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA39000.11003614264069814No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA36420.10275682858908272No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA36330.10250289902914264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA401.8189894E-1187.99983
GCAGCTC202.5505176E-587.99983
GCAGCTA302.0972948E-887.99983
AGCAGCC1050.087.99982
CAGCTGC6200.085.870771
GCTGCCC2050.085.853463
CAGCAGC5100.084.548831
AGCTGCC2450.084.4079742
GCAGCAA1100.083.999813
CAGCCCT200900.082.699671
AGCTGCT1650.082.666482
CAGCGTA80950.082.346951
GCTGCTT700.081.71413
AGCTGCA1400.081.71412
AGCAGCG1250.080.959822
AGCCCTT170000.080.933932
GCTGCAC501.8189894E-1279.199833
TGCTGCT501.8189894E-1279.199832
AGCTGCG950.078.7366642
GCGTAGT47000.078.638123
Produced by FastQC (version 0.11.5)