FastQCFastQC Report
Tue 7 Mar 2017
1SN_14A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_14A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3544290
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGC156.9372053E-493.8845752
TCACCGG133350.079.830978
CACCGGC134100.075.919299
GAGCTGC700.075.662341
CTTCACC149400.074.4907466
CCTTCAC166550.070.6530765
GTGTGGC55400.068.9661567
CGGGGCA14950.068.444294
AGGCAGC1050.067.060412
CGTAGTG29300.066.641854
TTCACCG165050.066.545967
AGGCGCG6900.065.304565
CGGGCCC8950.065.030947
GGCGCGC6900.064.6243066
ACCTTCA66050.060.7595634
CAGCTGC1150.059.8719331
TGTGGCA63850.059.6185388
CAGCGTA13100.059.4324761
GCGTAGT18700.058.9858173
GGGCCCG10000.058.6720668
Produced by FastQC (version 0.11.5)