##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_14A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3544290
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.757893400370737	12.0	12.0	12.0	2.0	27.0
2	14.70570974722723	12.0	12.0	12.0	12.0	22.0
3	13.708670848040088	12.0	12.0	12.0	12.0	22.0
4	13.770388427583521	12.0	12.0	12.0	12.0	22.0
5	22.443971853319	22.0	22.0	22.0	22.0	27.0
6	26.884026137816036	27.0	22.0	32.0	22.0	32.0
7	34.99405917687322	37.0	37.0	37.0	27.0	41.0
8	37.61476007888745	41.0	37.0	41.0	27.0	41.0
9	38.54306673550979	41.0	41.0	41.0	32.0	41.0
10-11	38.89898484604815	41.0	41.0	41.0	37.0	41.0
12-13	39.0768705438889	41.0	41.0	41.0	37.0	41.0
14-15	39.03861845390755	41.0	41.0	41.0	37.0	41.0
16-17	38.94527959055269	41.0	41.0	41.0	37.0	41.0
18-19	38.876567380208726	41.0	41.0	41.0	37.0	41.0
20-21	38.89325915768744	41.0	41.0	41.0	37.0	41.0
22-23	38.87811098978921	41.0	41.0	41.0	37.0	41.0
24-25	38.89143114699982	41.0	41.0	41.0	37.0	41.0
26-27	38.85996518343589	41.0	41.0	41.0	37.0	41.0
28-29	38.808571251223796	41.0	41.0	41.0	34.5	41.0
30-31	38.687151869626916	41.0	41.0	41.0	32.0	41.0
32-33	38.740369439295314	41.0	41.0	41.0	32.0	41.0
34-35	38.7133373679919	41.0	41.0	41.0	32.0	41.0
36-37	38.638507007045135	41.0	41.0	41.0	32.0	41.0
38-39	38.55165491537092	41.0	41.0	41.0	32.0	41.0
40-41	38.59314630010524	41.0	41.0	41.0	32.0	41.0
42-43	38.339037578753434	41.0	41.0	41.0	32.0	41.0
44-45	38.44688865753085	41.0	41.0	41.0	32.0	41.0
46-47	38.4609717319971	41.0	41.0	41.0	32.0	41.0
48-49	38.407329394603714	41.0	41.0	41.0	32.0	41.0
50-51	38.299317916987604	41.0	41.0	41.0	32.0	41.0
52-53	38.25821786591955	41.0	41.0	41.0	32.0	41.0
54-55	38.136609165728544	41.0	39.0	41.0	32.0	41.0
56-57	37.92004731554134	41.0	37.0	41.0	29.5	41.0
58-59	37.92023211983218	41.0	37.0	41.0	29.5	41.0
60-61	37.927449080069636	41.0	37.0	41.0	29.5	41.0
62-63	37.92010924613957	41.0	37.0	41.0	32.0	41.0
64-65	37.89791114722554	41.0	37.0	41.0	29.5	41.0
66-67	37.77551427789487	41.0	37.0	41.0	27.0	41.0
68-69	37.78115433556509	41.0	37.0	41.0	27.0	41.0
70-71	37.750987785988166	41.0	37.0	41.0	27.0	41.0
72-73	37.6382149880512	41.0	37.0	41.0	27.0	41.0
74-75	37.53108154242457	41.0	37.0	41.0	27.0	41.0
76-77	36.488889312104824	39.0	34.5	41.0	24.5	41.0
78-79	37.00804110273143	41.0	37.0	41.0	27.0	41.0
80-81	37.6581319530851	41.0	37.0	41.0	27.0	41.0
82-83	37.83956857367767	41.0	37.0	41.0	32.0	41.0
84-85	37.75106946666328	41.0	37.0	41.0	27.0	41.0
86-87	37.60646208408454	41.0	37.0	41.0	27.0	41.0
88-89	37.53893417299375	41.0	37.0	41.0	27.0	41.0
90-91	37.37118506104184	41.0	37.0	41.0	27.0	41.0
92-93	37.28202192822822	41.0	37.0	41.0	27.0	41.0
94-95	37.115477570966256	41.0	37.0	41.0	27.0	41.0
96-97	37.06061185738187	41.0	37.0	41.0	27.0	41.0
98-99	36.94145780960362	41.0	37.0	41.0	27.0	41.0
100	36.801323255151246	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5033124537241296
1101	2	-0.4511722523209052
1101	3	-0.07836839340584234
1101	4	0.13738605845937713
1101	5	-1.135918339485297
1101	6	-0.816695153715763
1101	7	-0.38338615827223776
1101	8	-0.2037163831960882
1101	9	-0.28800795017697567
1101	10-11	-0.24716433646623415
1101	12-13	-0.2027281899582647
1101	14-15	-0.13118537625067717
1101	16-17	-0.1545213150323832
1101	18-19	-0.025021822046312536
1101	20-21	0.003354398782938972
1101	22-23	-0.01480203287958659
1101	24-25	-0.07875992100262863
1101	26-27	0.0052268342930616996
1101	28-29	-0.06399752700430383
1101	30-31	-0.10901940007530442
1101	32-33	-0.09025158276500633
1101	34-35	-0.08356320252555349
1101	36-37	-0.04234154434116277
1101	38-39	-0.02273551914812799
1101	40-41	-0.022160258561051194
1101	42-43	0.08768335177497022
1101	44-45	-0.07674196965348301
1101	46-47	0.05060158536477388
1101	48-49	-0.10090822402252542
1101	50-51	0.08105383180377146
1101	52-53	-0.10339398525228916
1101	54-55	-0.06325725113786262
1101	56-57	0.13924737910591034
1101	58-59	-0.14372150752281954
1101	60-61	-0.0653380412920157
1101	62-63	-0.14721633554173508
1101	64-65	-0.1547119191618549
1101	66-67	-0.12783800355803976
1101	68-69	0.07627505005086732
1101	70-71	-0.09274902597311296
1101	72-73	-0.15348376518863205
1101	74-75	0.030048170297312993
1101	76-77	-0.46660073144311554
1101	78-79	-0.2154926492270519
1101	80-81	-0.16384760869929238
1101	82-83	-0.04178510202712005
1101	84-85	-0.09655341027218611
1101	86-87	-0.15280442247055248
1101	88-89	-0.322182491711807
1101	90-91	-0.26192943611089703
1101	92-93	-0.08655760457656925
1101	94-95	-0.14206770292290827
1101	96-97	-0.21487821515030703
1101	98-99	-0.38500420465661733
1101	100	-0.4802226081045049
1102	1	1.5003042425075659
1102	2	0.9309712156555108
1102	3	-0.005318174128120745
1102	4	0.14644598791616836
1102	5	-0.7998911629239736
1102	6	-0.1918240338342585
1102	7	-0.2221089260443847
1102	8	-0.3104734931834727
1102	9	-0.31958640287333395
1102	10-11	-0.2414204985090862
1102	12-13	-0.3592002510075005
1102	14-15	-0.2241144966226969
1102	16-17	-0.3417733367762139
1102	18-19	-0.19698420438017195
1102	20-21	-0.12954268509764688
1102	22-23	-0.11575923499911056
1102	24-25	-0.20100503283588012
1102	26-27	-0.17122799836850788
1102	28-29	-0.2860654337168782
1102	30-31	-0.23557927305881066
1102	32-33	-0.18332481087515617
1102	34-35	-0.15485367370642678
1102	36-37	-0.21923081796555266
1102	38-39	-0.2688054822952637
1102	40-41	-0.1886859773047007
1102	42-43	-0.10981100896351137
1102	44-45	-0.12468335683113452
1102	46-47	-0.3542361789705666
1102	48-49	-0.3633823673226928
1102	50-51	-0.2822679957687484
1102	52-53	-0.3609710874302152
1102	54-55	-0.26326346319991245
1102	56-57	-0.14716165775017487
1102	58-59	-0.24228000659181959
1102	60-61	-0.22338611055086233
1102	62-63	-0.26209339722024794
1102	64-65	-0.31808299951205043
1102	66-67	-0.28243767246319607
1102	68-69	-0.32747035947731007
1102	70-71	-0.4589909260896121
1102	72-73	-0.19783738399713968
1102	74-75	-0.40185038511589966
1102	76-77	-0.21268158586787678
1102	78-79	-0.28123935925444954
1102	80-81	-0.3054686059530738
1102	82-83	-0.399735411984814
1102	84-85	-0.29228227473951307
1102	86-87	-0.32671200715628856
1102	88-89	-0.3531700925973027
1102	90-91	-0.39268295520371055
1102	92-93	-0.3436021434479457
1102	94-95	-0.2984146151246918
1102	96-97	-0.29055676191504176
1102	98-99	-0.29769891649760893
1102	100	-0.34565784776101793
1103	1	1.546930451887583
1103	2	0.7285512875424942
1103	3	0.4225933488146403
1103	4	1.1296106171175015
1103	5	2.740785764491534
1103	6	-0.06783212862896093
1103	7	-0.5578309830667152
1103	8	-0.15167634863856705
1103	9	-0.1882013853354465
1103	10-11	-0.20343630847247596
1103	12-13	-0.3368070093517659
1103	14-15	-0.3524811744695526
1103	16-17	-0.3662947785540922
1103	18-19	-0.21170576747151415
1103	20-21	-0.18272234498611084
1103	22-23	-0.1440164470590375
1103	24-25	-0.31758713885082557
1103	26-27	-0.19648772930961655
1103	28-29	-0.35253377707901734
1103	30-31	-0.24734183909704655
1103	32-33	-0.3838003028871526
1103	34-35	-0.37045851389638784
1103	36-37	-0.228035221293581
1103	38-39	-0.05496746746398884
1103	40-41	-0.16031154006898163
1103	42-43	-0.07162897536953494
1103	44-45	-0.4241794971609423
1103	46-47	-0.5069779757112869
1103	48-49	-0.3597771717026603
1103	50-51	-0.29864145348592785
1103	52-53	-0.199940406973532
1103	54-55	-0.1798671369785083
1103	56-57	-0.2210323955095319
1103	58-59	-0.33316570745652996
1103	60-61	-0.19795794663919253
1103	62-63	-0.1744670568483997
1103	64-65	-0.43230631388911434
1103	66-67	-0.4173400710321289
1103	68-69	-0.4827335404262527
1103	70-71	-0.4499140055157156
1103	72-73	-0.18896091430214312
1103	74-75	-0.29507775273071246
1103	76-77	-0.4984727056227811
1103	78-79	-0.4955552439749624
1103	80-81	-0.2967921699655349
1103	82-83	-0.3588476044043105
1103	84-85	-0.35304652865341524
1103	86-87	-0.3600530790199059
1103	88-89	-0.29215929035992616
1103	90-91	-0.6491719527240463
1103	92-93	-0.3717725838474948
1103	94-95	-0.3136232258770306
1103	96-97	-0.17646965401448256
1103	98-99	-0.22986350231311548
1103	100	-0.40491058524003165
1104	1	1.6777218506905083
1104	2	-0.5787137644576497
1104	3	-0.018375261628587936
1104	4	0.13061859950433607
1104	5	-1.1714411376489124
1104	6	-0.650970781153557
1104	7	-0.3993939620715068
1104	8	-0.2176205647712166
1104	9	-0.23713210928279693
1104	10-11	-0.4160940662421808
1104	12-13	-0.22004068126866372
1104	14-15	-0.2641510337120181
1104	16-17	-0.26720556263934014
1104	18-19	-0.17879571095249958
1104	20-21	-0.2159322383711313
1104	22-23	-0.15281071444341165
1104	24-25	-0.18107705050078948
1104	26-27	-0.28299658610222167
1104	28-29	-0.36432120268820967
1104	30-31	-0.2500384761873917
1104	32-33	-0.25633458281447474
1104	34-35	-0.249681571682963
1104	36-37	-0.2853362726646793
1104	38-39	-0.3283931107632725
1104	40-41	-0.1995565385247957
1104	42-43	-0.37706469091386907
1104	44-45	-0.2569875238838435
1104	46-47	-0.5146605256793677
1104	48-49	-0.5456821864150356
1104	50-51	-0.30866869932562224
1104	52-53	-0.18828513694982973
1104	54-55	-0.2463797642315555
1104	56-57	-0.3116006550536241
1104	58-59	-0.3773591983913178
1104	60-61	-0.37279599683746767
1104	62-63	-0.4051079214396083
1104	64-65	-0.415345399732459
1104	66-67	-0.6638419742620343
1104	68-69	-0.48277996066774875
1104	70-71	-0.3401301343707246
1104	72-73	-0.3194321081739133
1104	74-75	-0.4856996323904994
1104	76-77	-0.7855046901165181
1104	78-79	-0.6907902535526915
1104	80-81	-0.6361255563505708
1104	82-83	-0.5100065946787922
1104	84-85	-0.4269750263238805
1104	86-87	-0.6682754300819838
1104	88-89	-0.5916361626587943
1104	90-91	-0.5624627310115002
1104	92-93	-0.5356076157827232
1104	94-95	-0.4473016794956308
1104	96-97	-0.5104865017200879
1104	98-99	-0.6830501097892352
1104	100	-0.5192559556261926
1105	1	1.6300566510852104
1105	2	0.19374897904513055
1105	3	-0.08275524032515769
1105	4	-0.299772156702085
1105	5	-1.1779876743711917
1105	6	0.3713396199521277
1105	7	0.10962732175422474
1105	8	-0.2205645918056618
1105	9	-0.16729404557508332
1105	10-11	-0.26342952420057486
1105	12-13	-0.20793655707816328
1105	14-15	-0.2002156763077707
1105	16-17	-0.27924581942409077
1105	18-19	-0.23854997408906797
1105	20-21	-0.06345490798172904
1105	22-23	-0.1469145429344607
1105	24-25	-0.14443987822073723
1105	26-27	-0.15958734908848982
1105	28-29	-0.2626805404194599
1105	30-31	-0.24081323529852483
1105	32-33	-0.22611105608389437
1105	34-35	-0.1335698111766419
1105	36-37	-0.30161291420506586
1105	38-39	-0.2605583699985772
1105	40-41	-0.11315246440715754
1105	42-43	-0.17756844188591714
1105	44-45	-0.2372908363711801
1105	46-47	-0.3071531801619045
1105	48-49	-0.30338563703159593
1105	50-51	-0.26216980738172424
1105	52-53	-0.23127290422292646
1105	54-55	-0.16395900474139324
1105	56-57	-0.13181911527632906
1105	58-59	-0.19625318850417273
1105	60-61	-0.34766823139411684
1105	62-63	-0.41024115568912123
1105	64-65	-0.4764882556500396
1105	66-67	-0.2836638133002438
1105	68-69	-0.461030683987822
1105	70-71	-0.43634252662830875
1105	72-73	-0.3844560193954578
1105	74-75	-0.35911836527750296
1105	76-77	-0.4689142661227166
1105	78-79	-0.3741695040280959
1105	80-81	-0.5422546115989846
1105	82-83	-0.38635388551635685
1105	84-85	-0.36821721724442824
1105	86-87	-0.3323554923422378
1105	88-89	-0.2984405072352416
1105	90-91	-0.5063304287870523
1105	92-93	-0.3564851202427235
1105	94-95	-0.31149506938761107
1105	96-97	-0.276932435280834
1105	98-99	-0.4259726720658321
1105	100	-0.4964577933737786
1106	1	1.220933931200797
1106	2	-0.6788749599109831
1106	3	-0.11289340178443119
1106	4	-0.2827559475635617
1106	5	-1.086148117752085
1106	6	-0.6277630078246439
1106	7	-0.11030224299202729
1106	8	0.013889422100788806
1106	9	0.0013075211577984192
1106	10-11	-0.03420171582058629
1106	12-13	-0.019605500097846118
1106	14-15	-0.02838441614883891
1106	16-17	0.024001087745851635
1106	18-19	0.07498567247043297
1106	20-21	-0.007604774786855728
1106	22-23	0.03113624043198371
1106	24-25	-0.050141013763450815
1106	26-27	-0.08218052905959894
1106	28-29	-0.1157908664082612
1106	30-31	0.0917548859765347
1106	32-33	0.12052577342667092
1106	34-35	-0.022287189720984202
1106	36-37	-0.1997893861212674
1106	38-39	0.06280886406319297
1106	40-41	-0.08052536489478967
1106	42-43	0.05354723447311471
1106	44-45	-0.01253013563070482
1106	46-47	-0.13544326278788077
1106	48-49	-0.07798012637600493
1106	50-51	-0.09801418384911642
1106	52-53	-0.09759407317723401
1106	54-55	-0.12828845886817675
1106	56-57	0.1543283359607983
1106	58-59	0.019688995891705474
1106	60-61	0.08984446726941542
1106	62-63	-0.02088178915489891
1106	64-65	-0.17172738559405332
1106	66-67	-0.19196394198003475
1106	68-69	-0.10792741281209572
1106	70-71	-0.37725705615692817
1106	72-73	-0.16578080041406906
1106	74-75	-0.3320038967024672
1106	76-77	-0.2916451560450568
1106	78-79	-0.2601199848841489
1106	80-81	-0.15757964094701293
1106	82-83	-0.22787174265921095
1106	84-85	-0.3675117359368656
1106	86-87	-0.19593657189795266
1106	88-89	-0.3114425277337034
1106	90-91	-0.27596397357665126
1106	92-93	-0.2692735210012529
1106	94-95	-0.19956340512899118
1106	96-97	-0.08043331572150691
1106	98-99	-0.3236708276554978
1106	100	-0.39506319792498346
1107	1	1.2886728593360424
1107	2	-0.5787137644576497
1107	3	-0.1864828504854561
1107	4	-0.22320784942297678
1107	5	-1.0833847815810316
1107	6	-0.12999735816284286
1107	7	-0.1986254702367276
1107	8	-0.05143420550128042
1107	9	-0.055896118248533355
1107	10-11	-0.05762464581310667
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2221	4	-0.24771811789269904
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2221	9	0.34211219303999485
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2221	12-13	0.39303352775345957
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2221	18-19	0.2606449924193015
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2221	78-79	0.6479335638276069
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2221	84-85	0.3400758361184728
2221	86-87	0.5220120914827149
2221	88-89	0.4953283577724008
2221	90-91	0.47265406255881004
2221	92-93	0.49990203804996725
2221	94-95	0.5657380721464804
2221	96-97	0.6427884185320352
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2221	100	0.6473983260392231
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2222	2	1.7400322650868194
2222	3	0.5370448187359003
2222	4	0.8395134372146256
2222	5	3.3270785566666774
2222	6	0.26029941510013543
2222	7	0.44608917922448654
2222	8	0.5129425476811491
2222	9	0.30700807500576843
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2222	12-13	0.35968148278484335
2222	14-15	0.21632351624091228
2222	16-17	0.3066453886634193
2222	18-19	0.3379414179222806
2222	20-21	0.38062591619286223
2222	22-23	0.4068104062698197
2222	24-25	0.4111767701765885
2222	26-27	0.3647672624085132
2222	28-29	0.3192930578433746
2222	30-31	0.324987533538426
2222	32-33	0.34580771811027233
2222	34-35	0.4369175749542791
2222	36-37	0.3832474938619015
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2222	40-41	0.25681430572335984
2222	42-43	0.5764338274710212
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2222	48-49	0.4414001282103328
2222	50-51	0.37452750188958106
2222	52-53	0.42464733486201567
2222	54-55	0.4887063561890699
2222	56-57	0.5529192687136302
2222	58-59	0.6036486906967085
2222	60-61	0.6042368789120331
2222	62-63	0.5551244190166997
2222	64-65	0.7045359421306756
2222	66-67	0.7042192911447387
2222	68-69	0.7610562958575926
2222	70-71	0.6411548493274211
2222	72-73	0.7139543173328136
2222	74-75	0.6843755195759016
2222	76-77	0.813264731813355
2222	78-79	0.7796206049299244
2222	80-81	0.5614145465723013
2222	82-83	0.4853691686785808
2222	84-85	0.43391038513341584
2222	86-87	0.6051031308129069
2222	88-89	0.6548697105684553
2222	90-91	0.45984061516528385
2222	92-93	0.6041866150289295
2222	94-95	0.7184450149348933
2222	96-97	0.8095907100735644
2222	98-99	0.8434558379745525
2222	100	0.7194674470432432
2223	1	1.1923421989304455
2223	2	-1.0485331439671857
2223	3	-0.19543864492915652
2223	4	-0.02105237205045718
2223	5	-1.0609172028325133
2223	6	0.013027678487965488
2223	7	0.35074836239130747
2223	8	0.2035957639928725
2223	9	0.08064105224070062
2223	10-11	0.17245048181688105
2223	12-13	0.0946684364562671
2223	14-15	0.08967782579194505
2223	16-17	0.22141074025122265
2223	18-19	0.06447692205206579
2223	20-21	0.08279566015644235
2223	22-23	0.12101361719407322
2223	24-25	0.10765283072608156
2223	26-27	0.12417306144335072
2223	28-29	0.08540451386574688
2223	30-31	0.03419984788330055
2223	32-33	0.06358539809375685
2223	34-35	0.1644126581599039
2223	36-37	0.05556155062478041
2223	38-39	0.034263330639021206
2223	40-41	0.12111971058580195
2223	42-43	0.27274747494505647
2223	44-45	0.2947201487003852
2223	46-47	0.2921823782459043
2223	48-49	0.24453421707692513
2223	50-51	0.20061694235581484
2223	52-53	0.1377662460984297
2223	54-55	0.19303601644775625
2223	56-57	0.2765343962976843
2223	58-59	0.3573466469758131
2223	60-61	0.28263165753887165
2223	62-63	0.20013388752138894
2223	64-65	0.1794726740480641
2223	66-67	0.27662024778341276
2223	68-69	0.3796819773314084
2223	70-71	0.35462823746152594
2223	72-73	0.38913911894218245
2223	74-75	0.33120572154040673
2223	76-77	0.24386728234987487
2223	78-79	0.38986010797354
2223	80-81	0.35490217693197934
2223	82-83	0.12577586443882893
2223	84-85	0.09461294305054935
2223	86-87	0.3016821056015786
2223	88-89	0.31373017267411285
2223	90-91	0.3406597592266749
2223	92-93	0.3664126885043686
2223	94-95	0.443208874611571
2223	96-97	0.39592120272392606
2223	98-99	0.3365465726144734
2223	100	0.5426873267194239
2224	1	1.4289849653220585
2224	2	-0.9924212593715964
2224	3	0.05380552764932922
2224	4	-0.15354057805928534
2224	5	-1.1077512267675758
2224	6	0.5825185385895288
2224	7	0.3406047788996531
2224	8	0.2415705576227296
2224	9	0.237144963015119
2224	10-11	0.31547629379770115
2224	12-13	0.2719830986385716
2224	14-15	0.25231848598664186
2224	16-17	0.18747015571833003
2224	18-19	0.23554780538188425
2224	20-21	0.21187335304858124
2224	22-23	0.2802998978985798
2224	24-25	0.2960108944796431
2224	26-27	0.2129459210721265
2224	28-29	0.21504967028486277
2224	30-31	0.2476457884338359
2224	32-33	0.21156154367434254
2224	34-35	0.25030511166583835
2224	36-37	0.10444800969190737
2224	38-39	0.2636459832928324
2224	40-41	0.2266375867739967
2224	42-43	0.4037901412448761
2224	44-45	0.31271848682879977
2224	46-47	0.298954781690135
2224	48-49	0.13665121773866673
2224	50-51	0.21850755926436705
2224	52-53	0.17161521818105285
2224	54-55	0.20156378164158895
2224	56-57	0.3600352410692267
2224	58-59	0.25302398219397304
2224	60-61	0.2711096377403024
2224	62-63	0.29640948069965134
2224	64-65	0.13884625835405018
2224	66-67	0.2974220059518444
2224	68-69	0.40919067369514295
2224	70-71	0.35536874672791896
2224	72-73	0.39107236894474795
2224	74-75	0.2889938273783841
2224	76-77	0.51034151345754
2224	78-79	0.18618023251113414
2224	80-81	0.29661748615315986
2224	82-83	0.2539898110359857
2224	84-85	0.09535760304284935
2224	86-87	0.4828609947724587
2224	88-89	0.34039670888939355
2224	90-91	0.30471714123338955
2224	92-93	0.3100648054931696
2224	94-95	0.38389569325014605
2224	96-97	0.3522250287444777
2224	98-99	0.33539148489185067
2224	100	0.5564371004060362
2225	1	1.3805459977672783
2225	2	1.6741711007239903
2225	3	1.0725040331955729
2225	4	1.0635356664271676
2225	5	3.209540804001289
2225	6	-0.11301652816749552
2225	7	0.14154876957864104
2225	8	0.3688755474575913
2225	9	0.27057697547484594
2225	10-11	0.2644475936276933
2225	12-13	0.1744836633685125
2225	14-15	0.19486137573947104
2225	16-17	0.2454040169255407
2225	18-19	0.19007961453883127
2225	20-21	0.13085362911235876
2225	22-23	0.18234778699750365
2225	24-25	0.19174510284716462
2225	26-27	0.17224405092434125
2225	28-29	0.06563698942266427
2225	30-31	0.26806820642911333
2225	32-33	0.28493333808886945
2225	34-35	0.2297985786321135
2225	36-37	0.23308457328049315
2225	38-39	0.18272434403043292
2225	40-41	0.2046621614431885
2225	42-43	0.39077162999188886
2225	44-45	0.35518766446311645
2225	46-47	0.3869929098778826
2225	48-49	0.2194361685774453
2225	50-51	0.31582984766897937
2225	52-53	0.25745387186487534
2225	54-55	0.30785041478112873
2225	56-57	0.31236668404339696
2225	58-59	0.34373566080913776
2225	60-61	0.37498190177235813
2225	62-63	0.37353078469080714
2225	64-65	0.28988179472860054
2225	66-67	0.3962114032958226
2225	68-69	0.4866739993005851
2225	70-71	0.4586444776073506
2225	72-73	0.3632201855204613
2225	74-75	0.4266045441692867
2225	76-77	0.5932004736411116
2225	78-79	0.3647514063171755
2225	80-81	0.30584636228113027
2225	82-83	0.2544508090029609
2225	84-85	0.29360010402369596
2225	86-87	0.2893167496677691
2225	88-89	0.3848468997086698
2225	90-91	0.4103665212193661
2225	92-93	0.4660131951211639
2225	94-95	0.48170662166617007
2225	96-97	0.595796915893601
2225	98-99	0.46868201764024064
2225	100	0.5976094797618927
2226	1	1.3394294228568082
2226	2	1.6106262233328348
2226	3	0.7776741533397686
2226	4	1.0221444168206197
2226	5	3.0776420149455177
2226	6	-0.2784826437030965
2226	7	0.15961672261371973
2226	8	0.2747512103886862
2226	9	0.27606749994019
2226	10-11	0.18858437271796902
2226	12-13	0.28094623931234963
2226	14-15	0.20341801217706745
2226	16-17	0.24117328424192408
2226	18-19	0.25276706145980654
2226	20-21	0.22499221957090043
2226	22-23	0.2979285235414224
2226	24-25	0.31032231761604834
2226	26-27	0.19052981356977483
2226	28-29	0.19614310353292552
2226	30-31	0.16115590634774435
2226	32-33	0.10931450342224736
2226	34-35	0.12051115903012999
2226	36-37	0.17299762760372772
2226	38-39	0.25328545246903644
2226	40-41	0.18573707488354074
2226	42-43	0.35824420706412496
2226	44-45	0.2331704831896957
2226	46-47	0.22065154713505564
2226	48-49	0.1874469595811803
2226	50-51	0.1391850658943099
2226	52-53	0.2787079029016226
2226	54-55	0.3839672272480925
2226	56-57	0.4258857271658343
2226	58-59	0.21304336179677108
2226	60-61	0.33780995244315193
2226	62-63	0.38953912292374326
2226	64-65	0.30201796211090937
2226	66-67	0.2340997339269535
2226	68-69	0.47464502928615815
2226	70-71	0.5770270445240442
2226	72-73	0.3663525163653958
2226	74-75	0.5541971763546343
2226	76-77	0.5252913865113342
2226	78-79	0.4143603289390825
2226	80-81	0.2900622179893162
2226	82-83	0.167188736678888
2226	84-85	0.19465114303641684
2226	86-87	0.3576688796586822
2226	88-89	0.26231229069696127
2226	90-91	0.31415450798746747
2226	92-93	0.2279772825181965
2226	94-95	0.5571784682521823
2226	96-97	0.5043431592345939
2226	98-99	0.4448286731417497
2226	100	0.32696403876795443
2227	1	1.3740715647429802
2227	2	1.923193641525506
2227	3	0.6175979778852039
2227	4	0.10775335350199633
2227	5	3.2085164465079288
2227	6	0.24715398415935041
2227	7	0.2562521255218897
2227	8	0.5356118210671852
2227	9	0.3719671396682429
2227	10-11	0.3160004208543654
2227	12-13	0.1865027090128919
2227	14-15	0.25682661176130495
2227	16-17	0.25986819156491947
2227	18-19	0.09674731790464364
2227	20-21	0.18057717600571266
2227	22-23	0.3126983887766812
2227	24-25	0.26776822617486573
2227	26-27	0.2544555276775995
2227	28-29	0.19817601374066385
2227	30-31	0.26375652529802096
2227	32-33	0.35978474454405074
2227	34-35	0.2567510286977637
2227	36-37	0.3218319445076503
2227	38-39	0.25882447929498653
2227	40-41	0.2687254790779292
2227	42-43	0.45337739798848986
2227	44-45	0.2971283159051197
2227	46-47	0.3704395396183173
2227	48-49	0.2677510387925892
2227	50-51	0.24581439591498366
2227	52-53	0.43577723313971006
2227	54-55	0.31249556747210505
2227	56-57	0.45268588864831827
2227	58-59	0.5246406498003822
2227	60-61	0.4423263722165558
2227	62-63	0.31185705859659407
2227	64-65	0.3324476744912488
2227	66-67	0.40208726597762023
2227	68-69	0.4674254580843993
2227	70-71	0.3876861232932072
2227	72-73	0.47360667683688007
2227	74-75	0.3522655632939191
2227	76-77	0.5089237440813648
2227	78-79	0.45836336488870444
2227	80-81	0.3877125194842179
2227	82-83	0.268596075237177
2227	84-85	0.3630720292233818
2227	86-87	0.4117894252401797
2227	88-89	0.397244834209026
2227	90-91	0.37084039146412096
2227	92-93	0.4304507117823988
2227	94-95	0.716257089168117
2227	96-97	0.579234165975052
2227	98-99	0.6995988546217689
2227	100	0.7502209742871315
2228	1	1.4722946026038457
2228	2	1.4345499355779783
2228	3	-0.2347345766606299
2228	4	-0.021753848439812984
2228	5	3.1219703076479064
2228	6	0.09024771135770493
2228	7	0.2088384616092327
2228	8	0.23559388684401483
2228	9	0.11401454006574596
2228	10-11	0.2765249845982467
2228	12-13	0.2594107310154854
2228	14-15	0.2635868311975784
2228	16-17	0.08240094608962067
2228	18-19	0.040223559972439205
2228	20-21	0.10678742897653137
2228	22-23	0.24072024396151193
2228	24-25	0.22770783943440165
2228	26-27	0.1576996566762503
2228	28-29	0.179536951037214
2228	30-31	0.1475054166927876
2228	32-33	0.1569850403358899
2228	34-35	0.17425261943879633
2228	36-37	0.2240019855106894
2228	38-39	0.09747764370267475
2228	40-41	0.18466998616931818
2228	42-43	0.3246364541570088
2228	44-45	0.20768936228105161
2228	46-47	0.12168228301397477
2228	48-49	0.22104761925213978
2228	50-51	0.2352359066644567
2228	52-53	0.2827683372736729
2228	54-55	0.2103181562403691
2228	56-57	0.22652943834699357
2228	58-59	0.45157515057793773
2228	60-61	0.3023610614262964
2228	62-63	0.2424533346657114
2228	64-65	0.14550526300155298
2228	66-67	0.37763358417987547
2228	68-69	0.3242577829247679
2228	70-71	0.4281258311373364
2228	72-73	0.5107140586122654
2228	74-75	0.21524023673136838
2228	76-77	0.4010990179467768
2228	78-79	0.3351621306210646
2228	80-81	0.2982504724768944
2228	82-83	0.23791184777225993
2228	84-85	0.22358578329851042
2228	86-87	0.2749901190243733
2228	88-89	0.36167389944292694
2228	90-91	0.33034393285901587
2228	92-93	0.31912758375906947
2228	94-95	0.5568645925522802
2228	96-97	0.4248244353077766
2228	98-99	0.5113769714300744
2228	100	0.5263114610416011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	23.0
13	52.0
14	193.0
15	633.0
16	2364.0
17	5772.0
18	9518.0
19	12125.0
20	14303.0
21	16576.0
22	18898.0
23	21813.0
24	24293.0
25	27544.0
26	31665.0
27	35733.0
28	40343.0
29	46042.0
30	52506.0
31	60122.0
32	70936.0
33	84860.0
34	103801.0
35	134110.0
36	188462.0
37	309099.0
38	758863.0
39	1471893.0
40	1748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.37428529065324	21.415221551147575	18.574456725442793	35.63603643275639
2	18.393241890392623	51.08863599682625	14.449073776236798	16.069048336544334
3	40.58739550093249	15.603435384802033	23.278569191572924	20.530599922692556
4	18.798207821594733	16.419423918471683	21.493529028380863	43.28883923155272
5	17.65338050780269	35.19474986527626	26.20386593647811	20.94800369044294
6	17.643844042107165	28.43864356471959	30.284683251088367	23.632829142084873
7	20.449483535489478	26.463381946736863	35.78138357752893	17.30575094024473
8	21.1753270753804	28.123234836878474	33.42720262732451	17.274235460416616
9	21.09192532213786	28.25420606101645	27.189112629045592	23.464755987800096
10-11	19.66505280324127	30.154205778872495	28.719052334882306	21.46168908300393
12-13	24.263590168976016	26.67174525786547	27.290021414726223	21.774643158432294
14-15	21.84122913192769	27.499837767225593	28.492758775382377	22.16617432546434
16-17	20.905611561130723	29.55731613383781	28.421757248983575	21.115315056047898
18-19	21.373589632902497	29.625214076726223	29.724020889938462	19.277175400432807
20-21	22.631881138394434	28.973842433886617	29.890796182027994	18.503480245690955
22-23	21.74970163276707	30.227436242519655	28.654963335392985	19.367898789320286
24-25	22.201146068747196	28.387631937567186	26.68233976339408	22.72888223029154
26-27	20.248357216819166	26.635066543651902	29.95441964399076	23.162156595538175
28-29	19.731942622454753	27.986979578495312	30.627361817080683	21.65371598196925
30-31	20.074863544253112	30.138579810545657	28.16736972632946	21.619186918871772
32-33	20.81009172499993	30.015080594420883	28.65510440737073	20.519723273208456
34-35	21.390955593362847	29.01986011302687	30.13970357956036	19.449480714049923
36-37	21.91701299837203	28.054546890914683	28.798616930330194	21.229823180383097
38-39	21.43082456611584	29.342594118271098	28.999349371265797	20.22723194434726
40-41	20.989960617813345	29.58854107591976	29.93762841250682	19.483869893760076
42-43	20.51517279994154	29.873953474133653	29.26766783667178	20.343205889253028
44-45	20.680573542232718	30.132918017430853	28.751893891301222	20.43461454903521
46-47	19.92321008697129	28.04304647953828	28.955166524157463	23.078576909332966
48-49	20.884239156502428	28.19243064196214	30.247440248963827	20.675889952571602
50-51	22.010233361265584	30.638731593633707	28.0950627629229	19.255972282177815
52-53	20.39692859356711	30.120156947632914	28.39779873792389	21.085115720876086
54-55	22.166108167835517	28.49842524917975	28.01292705265429	21.322539530330445
56-57	21.35117045342637	28.846649282721803	29.272806128193285	20.529374135658543
58-59	21.06139412245414	27.949270160325433	29.096036110471495	21.89329960674893
60-61	19.597049728104423	30.310851262321425	29.620232128722208	20.47186688085194
62-63	20.313738946306238	31.12045471604515	27.529531541791698	21.036274795856922
64-65	19.907490548520837	30.116428228925624	28.77363328274957	21.202447939803964
66-67	20.120531897784886	30.235858239591	29.50658665064089	20.13702321198322
68-69	20.820220693001986	29.90041729091017	29.23112950689701	20.04823250919084
70-71	19.686876638198342	30.888541851823636	29.20989817424646	20.214683335731557
72-73	20.110092571431796	30.695668243851376	28.58971754568616	20.604521639030665
74-75	19.951964991578002	29.19229239142395	30.303220108963995	20.55252250803405
76-77	19.661455467808786	30.460035719424766	28.65808102610114	21.220427786665315
78-79	19.536634981900466	29.437941026270426	28.509856699085006	22.515567292744105
80-81	20.708541350341388	29.544337829825313	28.459491719266655	21.287629100566647
82-83	20.38330667072954	28.225892915083133	29.7295226970705	21.66127771711683
84-85	20.460007505029214	29.193547932025876	28.397422332822654	21.949022230122253
86-87	21.522505212609577	28.59881668825068	29.46509738198624	20.413580717153508
88-89	21.234450341253115	28.249071041026554	29.181387527544306	21.33509109017603
90-91	21.0403917286678	29.07438443242511	29.13446698774649	20.7507568511606
92-93	21.2610141946624	28.84177084832223	29.609893659943175	20.287321297072193
94-95	20.732925353173695	29.485044959639307	29.616411185314973	20.165618501872025
96-97	20.818499614873502	29.280504699107578	29.439817283574428	20.461178402444496
98-99	21.733718177688623	29.829091298962556	28.937502292419637	19.499688230929184
100	21.23852732140993	30.812264233457192	27.660744465040953	20.288463980091922
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	11.5
2	19.0
3	24.5
4	36.5
5	45.5
6	51.5
7	57.0
8	73.5
9	88.0
10	86.5
11	100.0
12	132.5
13	156.5
14	187.0
15	265.0
16	340.0
17	428.5
18	625.0
19	953.5
20	1490.0
21	2351.5
22	3685.0
23	5730.5
24	8890.5
25	13391.0
26	19074.0
27	25617.0
28	32954.5
29	41483.0
30	51481.5
31	63306.5
32	76356.0
33	90281.5
34	106016.0
35	123097.0
36	139475.5
37	152619.0
38	162319.0
39	169578.0
40	173699.5
41	174178.0
42	171301.5
43	167348.0
44	165748.0
45	162649.5
46	152594.5
47	138882.0
48	127175.5
49	120195.0
50	115152.0
51	107471.0
52	95677.5
53	80559.0
54	65754.0
55	52661.5
56	40497.0
57	29661.5
58	20798.0
59	14929.0
60	11815.5
61	9815.5
62	7982.5
63	6195.5
64	4697.0
65	4314.0
66	5199.0
67	5042.0
68	3424.5
69	3101.0
70	4071.0
71	3882.5
72	2214.0
73	865.0
74	381.0
75	241.0
76	152.5
77	105.5
78	96.0
79	78.5
80	68.0
81	53.0
82	46.0
83	43.0
84	33.0
85	25.5
86	21.0
87	18.0
88	16.0
89	19.0
90	20.0
91	13.0
92	11.0
93	18.5
94	18.0
95	16.5
96	17.5
97	22.0
98	59.0
99	115.5
100	111.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.325371230909434
2	0.006263595811855125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.24465548812315E-4
30-31	0.010594505528610808
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.33574284271321E-4
40-41	0.001354290986347054
42-43	9.875038442113936E-5
44-45	0.0
46-47	0.0021019724683928234
48-49	0.0
50-51	0.0
52-53	0.0014248269752192965
54-55	0.02013097122413798
56-57	0.011483258988401062
58-59	0.014050768983350686
60-61	1.8339357106783024E-4
62-63	0.012752906788101426
64-65	9.875038442113936E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.3982236216562415E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3544290.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.60902183972387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.04400935440478	60.89918323197253
2	9.036074345900971	12.941288903619824
3	2.612552060340323	5.612468926389774
4	1.1849956278908813	3.394255111904617
5	0.6026108277428569	2.1576185962346157
6	0.36186816474680494	1.554781518747285
7	0.24243037755233426	1.2152141540530323
8	0.18002372141813447	1.0313058078959645
9	0.12395231129879185	0.7988493390191493
>10	0.5696148827529534	6.973293388590637
>50	0.028551889360927236	1.3940013120520311
>100	0.012352871111189878	1.5302498476944641
>500	8.029712377917582E-4	0.3709611499767118
>1k	1.6059424129941539E-4	0.12652871184931222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.821439554889696E-5	0.0
3	0.0	0.0	0.0	2.821439554889696E-5	0.0
4	0.0	0.0	0.0	2.821439554889696E-5	0.0
5	0.0	0.0	0.0	2.821439554889696E-5	0.0
6	0.0	0.0	0.0	2.821439554889696E-5	0.0
7	0.0	0.0	0.0	2.821439554889696E-5	0.0
8	0.0	0.0	0.0	2.821439554889696E-5	0.0
9	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
10-11	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
12-13	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
14-15	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
16-17	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
18-19	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
20-21	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
22-23	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
24-25	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
26-27	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
28-29	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
30-31	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
32-33	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
34-35	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
36-37	2.821439554889696E-5	0.0	0.0	2.821439554889696E-5	0.0
38-39	5.642879109779392E-5	0.0	0.0	2.821439554889696E-5	0.0
40-41	5.642879109779392E-5	0.0	0.0	2.821439554889696E-5	0.0
42-43	5.642879109779392E-5	0.0	0.0	2.821439554889696E-5	0.0
44-45	7.05359888722424E-5	0.0	0.0	2.821439554889696E-5	0.0
46-47	9.875038442113936E-5	0.0	0.0	2.821439554889696E-5	0.0
48-49	1.269647799700363E-4	0.0	0.0	2.821439554889696E-5	0.0
50-51	1.410719777444848E-4	0.0	0.0	2.821439554889696E-5	0.0
52-53	1.5517917551893326E-4	0.0	0.0	2.821439554889696E-5	0.0
54-55	1.9750076884227872E-4	0.0	0.0	3.9500153768455744E-4	0.0
56-57	2.2571516439117567E-4	0.0	1.5517917551893326E-4	7.617886798202179E-4	0.0
58-59	3.103583510378665E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
60-61	3.667871421356605E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
62-63	3.9500153768455744E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
64-65	4.5143032878235134E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
66-67	4.655375265567998E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
68-69	5.219663176545937E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
70-71	5.642879109779391E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
72-73	7.05359888722424E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
74-75	7.617886798202179E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
76-77	9.028606575647027E-4	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
78-79	0.0011003614264069813	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
80-81	0.0015941133485126782	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
82-83	0.0017210781284827145	0.0	3.103583510378665E-4	7.617886798202179E-4	0.0
84-85	0.0017775069195805084	0.0	3.103583510378665E-4	7.900030753691149E-4	0.0
86-87	0.001975007688422787	0.0	3.103583510378665E-4	7.900030753691149E-4	0.0
88	0.001975007688422787	0.0	3.103583510378665E-4	7.900030753691149E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAGC	15	6.9372053E-4	93.884575	2
TCACCGG	13335	0.0	79.83097	8
CACCGGC	13410	0.0	75.91929	9
GAGCTGC	70	0.0	75.66234	1
CTTCACC	14940	0.0	74.490746	6
CCTTCAC	16655	0.0	70.653076	5
GTGTGGC	5540	0.0	68.966156	7
CGGGGCA	1495	0.0	68.4442	94
AGGCAGC	105	0.0	67.06041	2
CGTAGTG	2930	0.0	66.64185	4
TTCACCG	16505	0.0	66.54596	7
AGGCGCG	690	0.0	65.30456	5
CGGGCCC	895	0.0	65.03094	7
GGCGCGC	690	0.0	64.624306	6
ACCTTCA	6605	0.0	60.759563	4
CAGCTGC	115	0.0	59.871933	1
TGTGGCA	6385	0.0	59.618538	8
CAGCGTA	1310	0.0	59.432476	1
GCGTAGT	1870	0.0	58.985817	3
GGGCCCG	1000	0.0	58.672066	8
>>END_MODULE