FastQCFastQC Report
Tue 7 Mar 2017
1SN_15A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_15A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2506325
Sequences flagged as poor quality0
Sequence length94
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA140430.5603024348398552No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG72850.2906646185151567No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA58150.23201300709205708No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA57880.23093573259653077No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT51980.2073952899165112No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG47790.19067758570815835No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG47770.1905977875973786No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG47010.187565459387749No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT38230.15253408875544872No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC36970.14750680777632588No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT35350.14104316080316798No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34550.1378512363719789No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA34290.13681386093184245No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA32100.1280759678014623No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC29320.11698403040308021No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA28940.11546786629826539No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA28150.11231584092246615No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC25740.10270016857350903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCG202.5503636E-587.999862
AGCTGCC750.087.999862
GCAGCGG401.8189894E-1187.999863
GCAGCAT302.096931E-887.999863
TGCAGCT158.971749E-487.999862
TGCTGCA450.087.9998552
CAGCTGC2850.087.9998551
CTGCTGC1100.083.999861
AGCAGCA950.083.3682862
CAGCAGC2600.082.922941
AGCAGCG850.082.8233952
CAGCCCT148750.082.143061
CAGCGTA55750.082.080591
CTGCAGC700.081.714161
AGCTGCT1100.079.999872
GCAGCAA550.079.999873
AGCCCTT127100.079.96842
CAGCTCT58950.078.968491
GCCCTTC114850.078.881893
CCCTTCA113000.078.2653664
Produced by FastQC (version 0.11.5)