FastQCFastQC Report
Tue 7 Mar 2017
1SN_15A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_15A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2506325
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCA650.081.42321
TCACCGG97650.080.898558
CACCGGC93250.080.386849
CTTCACC110850.073.805826
CCTTCAC123900.070.502415
CAGCGTA9400.070.3791051
CGGGCCC7400.068.504797
CGGGGCA10800.067.894
CGTAGTG21050.067.118664
GTGTGGC42800.066.349937
TTCACCG122350.066.216427
GGGCCCG7950.062.5846148
ACCTTCA50800.061.9994284
CAGCAGC601.0018994E-861.745931
GGCCCGA8200.060.6765569
TGTGGCA47650.060.1876268
AACGGGC8350.060.1486855
GGCTGCC457.9914225E-658.805651
TGTGTGG45700.058.6474086
CCGCGTA3000.058.217591
Produced by FastQC (version 0.11.5)