##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_15A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2506325 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.782146768675252 12.0 12.0 12.0 2.0 27.0 2 14.634253339052197 12.0 12.0 12.0 12.0 22.0 3 13.745416097273898 12.0 12.0 12.0 12.0 22.0 4 13.772651192483018 12.0 12.0 12.0 12.0 22.0 5 22.46257368856792 22.0 22.0 22.0 22.0 27.0 6 26.87899933169082 27.0 22.0 32.0 22.0 32.0 7 35.00331202058791 37.0 37.0 37.0 27.0 41.0 8 37.628571314574124 41.0 37.0 41.0 32.0 41.0 9 38.5899191046652 41.0 41.0 41.0 32.0 41.0 10-11 38.95394072995322 41.0 41.0 41.0 37.0 41.0 12-13 39.12236002912631 41.0 41.0 41.0 37.0 41.0 14-15 39.0819464754172 41.0 41.0 41.0 37.0 41.0 16-17 38.979575873041206 41.0 41.0 41.0 37.0 41.0 18-19 38.91727609148853 41.0 41.0 41.0 37.0 41.0 20-21 38.93236172483616 41.0 41.0 41.0 37.0 41.0 22-23 38.91623412765703 41.0 41.0 41.0 37.0 41.0 24-25 38.9312493391719 41.0 41.0 41.0 37.0 41.0 26-27 38.895223484584 41.0 41.0 41.0 37.0 41.0 28-29 38.85097762660469 41.0 41.0 41.0 37.0 41.0 30-31 38.721597159187255 41.0 41.0 41.0 32.0 41.0 32-33 38.77082601019421 41.0 41.0 41.0 32.0 41.0 34-35 38.75036976449583 41.0 41.0 41.0 32.0 41.0 36-37 38.67109113941727 41.0 41.0 41.0 32.0 41.0 38-39 38.57740935433354 41.0 41.0 41.0 32.0 41.0 40-41 38.62664598565629 41.0 41.0 41.0 32.0 41.0 42-43 38.35944580212063 41.0 41.0 41.0 32.0 41.0 44-45 38.47847565658883 41.0 41.0 41.0 32.0 41.0 46-47 38.48682832433942 41.0 41.0 41.0 32.0 41.0 48-49 38.4416504244262 41.0 41.0 41.0 32.0 41.0 50-51 38.33003481192583 41.0 41.0 41.0 32.0 41.0 52-53 38.293446580152214 41.0 41.0 41.0 32.0 41.0 54-55 38.158826768276256 41.0 39.0 41.0 32.0 41.0 56-57 37.93549220472205 41.0 37.0 41.0 29.5 41.0 58-59 37.93934266306245 41.0 37.0 41.0 29.5 41.0 60-61 37.93479057983302 41.0 37.0 41.0 32.0 41.0 62-63 37.9368350074312 41.0 37.0 41.0 32.0 41.0 64-65 37.92370921568432 41.0 37.0 41.0 32.0 41.0 66-67 37.80058531914257 41.0 37.0 41.0 27.0 41.0 68-69 37.81147157691042 41.0 37.0 41.0 27.0 41.0 70-71 37.79683261348788 41.0 37.0 41.0 27.0 41.0 72-73 37.67862029066462 41.0 37.0 41.0 27.0 41.0 74-75 37.56123527475487 41.0 37.0 41.0 27.0 41.0 76-77 36.52199096286395 39.0 34.5 41.0 24.5 41.0 78-79 37.03797771637757 41.0 37.0 41.0 27.0 41.0 80-81 37.697808743877985 41.0 37.0 41.0 27.0 41.0 82-83 37.88437154000379 41.0 37.0 41.0 32.0 41.0 84-85 37.79367380527266 41.0 37.0 41.0 27.0 41.0 86-87 37.66138230277399 41.0 37.0 41.0 27.0 41.0 88-89 37.565946156224754 41.0 37.0 41.0 27.0 41.0 90-91 37.408902875724415 41.0 37.0 41.0 27.0 41.0 92-93 37.29225619183466 41.0 37.0 41.0 27.0 41.0 94-95 37.137912481422006 41.0 37.0 41.0 27.0 41.0 96-97 37.0752035350563 41.0 37.0 41.0 27.0 41.0 98-99 36.95867694732327 41.0 37.0 41.0 27.0 41.0 100 36.82536702143577 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2369796017772163 1101 2 -0.5039524425523965 1101 3 0.045966579889549664 1101 4 0.19419976613854395 1101 5 -1.0093374327741138 1101 6 -0.7977625021814951 1101 7 -0.12453531708349175 1101 8 0.009746315897629643 1101 9 0.1310196376583903 1101 10-11 -0.02049181555130275 1101 12-13 0.007453091918108612 1101 14-15 0.022578766712413767 1101 16-17 -0.014903717850913267 1101 18-19 -0.04032095197064223 1101 20-21 0.09965958173647493 1101 22-23 0.07327310702202539 1101 24-25 0.09366528726805257 1101 26-27 0.14470333164008764 1101 28-29 -0.03664984071005506 1101 30-31 0.10788319051798823 1101 32-33 -0.007660132718889656 1101 34-35 -0.004873367606940349 1101 36-37 0.10727816255091227 1101 38-39 0.022623081996520966 1101 40-41 0.19062369301654059 1101 42-43 0.18409496100157696 1101 44-45 0.028020132920168805 1101 46-47 -0.0032048653874241495 1101 48-49 0.12404415936175894 1101 50-51 0.24699692874109047 1101 52-53 0.04092939136937446 1101 54-55 0.1822005026430702 1101 56-57 0.22281178282192826 1101 58-59 0.13471899209139337 1101 60-61 0.025415890335800384 1101 62-63 -0.030924107411443913 1101 64-65 0.20831788110682936 1101 66-67 0.17236434454157745 1101 68-69 0.1755502756985834 1101 70-71 0.09880952720115488 1101 72-73 0.04751753535126113 1101 74-75 0.13820638101369553 1101 76-77 -0.2231852103122236 1101 78-79 -0.07158953483631336 1101 80-81 0.011991013965229058 1101 82-83 -0.07254389018175544 1101 84-85 -0.009704358660037826 1101 86-87 0.053465436782659026 1101 88-89 -0.24448318355037202 1101 90-91 -0.16866770079695925 1101 92-93 0.024054371321192036 1101 94-95 0.03130748616771939 1101 96-97 -0.09901307277652194 1101 98-99 -0.18076590321690844 1101 100 -0.3269779524509815 1102 1 1.7785727875206927 1102 2 0.9344574687959089 1102 3 -0.05675806308061482 1102 4 -0.023032378892086314 1102 5 -0.7999687096780548 1102 6 -0.2774831993258111 1102 7 -0.5040918929109992 1102 8 -0.3197482464970065 1102 9 -0.4353122335230495 1102 10-11 -0.4116666791125496 1102 12-13 -0.4638269050216266 1102 14-15 -0.33169457557125526 1102 16-17 -0.45834507368159905 1102 18-19 -0.3039025892848315 1102 20-21 -0.1717753516177538 1102 22-23 -0.3134678421159123 1102 24-25 -0.3004912942463207 1102 26-27 -0.27887173509365226 1102 28-29 -0.31659185513301225 1102 30-31 -0.37937884328940896 1102 32-33 -0.4270055417099101 1102 34-35 -0.39813324490564383 1102 36-37 -0.5192764946752888 1102 38-39 -0.4417945246224946 1102 40-41 -0.3761687529433999 1102 42-43 -0.13337044781759744 1102 44-45 -0.0509712543417038 1102 46-47 -0.5383683126674157 1102 48-49 -0.641840044999654 1102 50-51 -0.5026534210523081 1102 52-53 -0.46122220779576395 1102 54-55 -0.4681400853993338 1102 56-57 -0.32792283548401713 1102 58-59 -0.2942372204721977 1102 60-61 -0.3106423996317744 1102 62-63 -0.4086300993021368 1102 64-65 -0.4498767189479551 1102 66-67 -0.40270423026638724 1102 68-69 -0.6904961066261492 1102 70-71 -0.6624157428584994 1102 72-73 -0.4387699578188773 1102 74-75 -0.5737611171150618 1102 76-77 -0.5168595742270554 1102 78-79 -0.5085319074500063 1102 80-81 -0.4895046522541975 1102 82-83 -0.626264113147819 1102 84-85 -0.6700192596604921 1102 86-87 -0.49048708238647265 1102 88-89 -0.5426509898745309 1102 90-91 -0.48753109675196526 1102 92-93 -0.5465347736789496 1102 94-95 -0.5581363812939344 1102 96-97 -0.5737328054038144 1102 98-99 -0.527305209996122 1102 100 -0.8946462371469508 1103 1 1.7557544190263474 1103 2 0.877738198212457 1103 3 0.43718284935465235 1103 4 1.1507244359020188 1103 5 2.852711702502763 1103 6 -0.20831797481066516 1103 7 -0.513966485725831 1103 8 -0.36133533586841793 1103 9 -0.4034294889027379 1103 10-11 -0.3575780487910407 1103 12-13 -0.39803095122325516 1103 14-15 -0.38384873662879215 1103 16-17 -0.366615416940661 1103 18-19 -0.18302943824546958 1103 20-21 -0.3851977089626999 1103 22-23 -0.405797777120128 1103 24-25 -0.4738607434158837 1103 26-27 -0.2714121008070052 1103 28-29 -0.33023528881903275 1103 30-31 -0.3833512639235508 1103 32-33 -0.42538797998626876 1103 34-35 -0.5568985895379797 1103 36-37 -0.4094073169795891 1103 38-39 -0.12445305685034924 1103 40-41 -0.144296736002282 1103 42-43 -0.11403703667611609 1103 44-45 -0.4387700884091146 1103 46-47 -0.49831304563333134 1103 48-49 -0.5385026496432488 1103 50-51 -0.32829335378175983 1103 52-53 -0.4816767947401175 1103 54-55 -0.5177600117249952 1103 56-57 -0.38006356130279784 1103 58-59 -0.6210972985114438 1103 60-61 -0.24246194735035687 1103 62-63 -0.3009624547005032 1103 64-65 -0.4180946873388365 1103 66-67 -0.6678202584241859 1103 68-69 -0.4422310472105977 1103 70-71 -0.5332475113112878 1103 72-73 -0.5321466107378399 1103 74-75 -0.6267559549066064 1103 76-77 -0.7856792156846311 1103 78-79 -0.6171019693485107 1103 80-81 -0.40793583070514217 1103 82-83 -0.4106616575553872 1103 84-85 -0.6531194727987568 1103 86-87 -0.46203852837712134 1103 88-89 -0.451560173608911 1103 90-91 -0.8070188182770011 1103 92-93 -0.6026132551646768 1103 94-95 -0.7497947799898697 1103 96-97 -0.42201480757847065 1103 98-99 -0.4310922858707613 1103 100 -0.4770976820914967 1104 1 1.3366711775661688 1104 2 -0.3757489561574747 1104 3 -0.13734997336164945 1104 4 0.09222849778590003 1104 5 -1.0910221814784116 1104 6 -0.3204747651386981 1104 7 -0.19231627877429247 1104 8 -0.20844699268126732 1104 9 0.10279291730358864 1104 10-11 -0.010413907591534155 1104 12-13 -0.038455220737411366 1104 14-15 -0.017704508718964007 1104 16-17 0.08247442226551982 1104 18-19 -0.0573580712198094 1104 20-21 -0.05166941906299627 1104 22-23 0.07820712196615887 1104 24-25 -0.0592342926302436 1104 26-27 0.029252504395252288 1104 28-29 -0.11057926435540111 1104 30-31 -0.10112325177326653 1104 32-33 -0.1183850952813188 1104 34-35 -0.15351745362909952 1104 36-37 -0.21972904932714954 1104 38-39 -0.1879436741706897 1104 40-41 -0.12619767360782674 1104 42-43 -0.17513915580202166 1104 44-45 -0.08429482119225185 1104 46-47 -0.321904971068399 1104 48-49 -0.23179948645511672 1104 50-51 -0.11649989808541505 1104 52-53 -0.01042040412909273 1104 54-55 -0.2581862990854731 1104 56-57 0.049279511502120954 1104 58-59 -0.13991962632749733 1104 60-61 -0.14422982531590378 1104 62-63 -0.0873960458493741 1104 64-65 -0.15309408911873845 1104 66-67 -0.594487035155197 1104 68-69 -0.4843730542665341 1104 70-71 -0.2287716224347065 1104 72-73 -0.11964480945331246 1104 74-75 -0.22427469692821234 1104 76-77 -0.45161690988960146 1104 78-79 -0.22881687171430798 1104 80-81 -0.27267266176106375 1104 82-83 -0.3160793914895095 1104 84-85 -0.08997464096179186 1104 86-87 -0.1403114802070462 1104 88-89 -0.30082980496061396 1104 90-91 -0.35137271101848455 1104 92-93 -0.1831799479410492 1104 94-95 -0.20490952105025428 1104 96-97 -0.2587338424527701 1104 98-99 -0.20241261571321445 1104 100 -0.2755894336860507 1105 1 1.7042340181509967 1105 2 0.09886105112795462 1105 3 -0.06543263205698757 1105 4 -0.19941091608836814 1105 5 -1.1342302450616515 1105 6 0.6862599028757437 1105 7 0.21039440558161715 1105 8 0.21280339846762075 1105 9 0.277862453216315 1105 10-11 0.11340686483217866 1105 12-13 0.14670670739690195 1105 14-15 -0.047666964431300585 1105 16-17 0.04031833148414421 1105 18-19 0.07490851424405065 1105 20-21 0.1076217260467871 1105 22-23 0.2665895343503024 1105 24-25 0.12679101597947806 1105 26-27 0.10130618473846909 1105 28-29 0.11928715341745288 1105 30-31 0.16680187827435589 1105 32-33 0.049868990235090394 1105 34-35 0.09643464752365816 1105 36-37 0.03474145969794762 1105 38-39 -0.06568119089023838 1105 40-41 0.1875968340310621 1105 42-43 0.22833580796603314 1105 44-45 0.17939088549142213 1105 46-47 -0.1416920899722598 1105 48-49 0.025904419988670213 1105 50-51 0.07093474776642239 1105 52-53 0.055275792852590655 1105 54-55 0.07245268655208292 1105 56-57 0.11706734153118958 1105 58-59 0.13443265813218375 1105 60-61 -0.053244838826685736 1105 62-63 -0.0957838997828162 1105 64-65 -0.06875457968701681 1105 66-67 0.12547648364126474 1105 68-69 -0.07839984278547263 1105 70-71 -0.19160061544177154 1105 72-73 0.0067637019466104675 1105 74-75 0.02526778649637862 1105 76-77 -0.1735348217761441 1105 78-79 -0.2306036939155831 1105 80-81 -0.3012849405629794 1105 82-83 -0.06561556848280503 1105 84-85 -0.11342976049268572 1105 86-87 -0.10642882667698217 1105 88-89 -0.11947305734940272 1105 90-91 -0.09744305355380334 1105 92-93 0.15652408222891268 1105 94-95 0.030767222092407565 1105 96-97 -0.0338927495061867 1105 98-99 -0.10604644148874343 1105 100 -0.008638797427195755 1106 1 1.2869440647676083 1106 2 -0.693742156694162 1106 3 -0.141463542244896 1106 4 -0.26883438292387574 1106 5 -1.1106138207417509 1106 6 -0.6684059469731771 1106 7 -0.21347721386831608 1106 8 -0.3797769320132929 1106 9 -0.3546154769965213 1106 10-11 0.03387316385030914 1106 12-13 -0.1299518394247201 1106 14-15 -0.08815633412027069 1106 16-17 -0.1423288427211702 1106 18-19 -0.020146133601976146 1106 20-21 -0.06797787778855024 1106 22-23 -0.10728935509977333 1106 24-25 -0.24428288186030045 1106 26-27 -0.2668931429576986 1106 28-29 -0.2939145828438612 1106 30-31 0.10232301846815517 1106 32-33 -0.2102823264606215 1106 34-35 -0.11697842220470278 1106 36-37 -0.24030006589848796 1106 38-39 -0.08023478468692957 1106 40-41 -0.06615819153275737 1106 42-43 0.07887024360054795 1106 44-45 -0.07770441313440557 1106 46-47 -0.38407419715260716 1106 48-49 -0.2200076649668148 1106 50-51 -0.1652278843522268 1106 52-53 -0.24925605538686568 1106 54-55 -0.23977388290199286 1106 56-57 -0.2846327913023927 1106 58-59 -0.2759223998143483 1106 60-61 -0.2839235547634118 1106 62-63 -0.49144459578564437 1106 64-65 -0.627946880656161 1106 66-67 -0.6304130220228927 1106 68-69 -0.6274783853699475 1106 70-71 -0.7021684971046938 1106 72-73 -0.5731684393483505 1106 74-75 -0.8337328764631877 1106 76-77 -0.5447587514750367 1106 78-79 -0.47610293952720184 1106 80-81 -0.5331520296549215 1106 82-83 -0.647696588908893 1106 84-85 -0.6687080424043685 1106 86-87 -0.5828892307520448 1106 88-89 -0.6240900109092067 1106 90-91 -0.5668880081736916 1106 92-93 -0.44613103863030545 1106 94-95 -0.4994086152464803 1106 96-97 -0.5800734619742514 1106 98-99 -0.7727262988759094 1106 100 -0.9154704226125716 1107 1 1.255722641724967 1107 2 -0.6329927599338543 1107 3 -0.15910432777408268 1107 4 -0.20032609700024118 1107 5 -1.2879200302486922 1107 6 -0.3969359007874438 1107 7 -0.44723641528717906 1107 8 -0.4586561494249253 1107 9 -0.3702844266531642 1107 10-11 -0.2759447325221558 1107 12-13 -0.3692269357241642 1107 14-15 -0.3904336310777694 1107 16-17 -0.23713371192493327 1107 18-19 -0.23353179727302376 1107 20-21 -0.13268467975493792 1107 22-23 -0.03126680083298794 1107 24-25 -0.12607165173045587 1107 26-27 -0.3425517088787231 1107 28-29 -0.28126379357257036 1107 30-31 -0.2924536816517076 1107 32-33 -0.16275783959641643 1107 34-35 -0.22038694331127573 1107 36-37 -0.21986106187799948 1107 38-39 -0.2534768190730432 1107 40-41 -0.19686955589944688 1107 42-43 -0.24906043567088432 1107 44-45 -0.41190921889577226 1107 46-47 -0.31027790919352327 1107 48-49 -0.2958279346465531 1107 50-51 -0.28604681992433 1107 52-53 -0.23729885752506874 1107 54-55 -0.2901312833168461 1107 56-57 -0.23209559117716339 1107 58-59 -0.469792608901642 1107 60-61 -0.3572195523578827 1107 62-63 -0.40833929297625104 1107 64-65 -0.2913424365494919 1107 66-67 -0.47110611365832256 1107 68-69 -0.6426337242374203 1107 70-71 -0.3346094514831748 1107 72-73 -0.3985853789632614 1107 74-75 -0.38151416872197785 1107 76-77 -0.4620399474963435 1107 78-79 -0.6978084990800326 1107 80-81 -0.5679330005713723 1107 82-83 -0.45333600233108484 1107 84-85 -0.2257040739504319 1107 86-87 -0.20399367774997756 1107 88-89 -0.11481775630034008 1107 90-91 -0.16258080242290873 1107 92-93 -0.1995793764478364 1107 94-95 -0.1683393754730531 1107 96-97 -0.48845795675223513 1107 98-99 -0.3891857382863577 1107 100 -0.5221478172118736 1108 1 1.7254818352075638 1108 2 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0.18971171684813015 2223 28-29 0.07450414353455415 2223 30-31 0.028483899709492277 2223 32-33 0.14825912898900384 2223 34-35 0.18238800757522 2223 36-37 0.02355095113939143 2223 38-39 0.16034844136904525 2223 40-41 0.11626597463696697 2223 42-43 0.3615646192463373 2223 44-45 0.20101480042210795 2223 46-47 0.2568029863899852 2223 48-49 -0.002409539562762575 2223 50-51 0.07823615339213319 2223 52-53 0.2099485440404365 2223 54-55 0.26737681703723837 2223 56-57 0.40611652922940067 2223 58-59 0.3895053516172169 2223 60-61 0.33159271565805426 2223 62-63 0.321674844975 2223 64-65 0.3050651195736265 2223 66-67 0.2710331387693472 2223 68-69 0.0873203472064148 2223 70-71 0.32683846385390325 2223 72-73 0.3344934698945963 2223 74-75 0.30291730279990503 2223 76-77 0.3288419566768752 2223 78-79 0.3510034523691772 2223 80-81 0.2505966443807779 2223 82-83 0.2435588636262409 2223 84-85 0.20964944889949066 2223 86-87 0.30881144641013236 2223 88-89 0.22744972594503565 2223 90-91 0.3227206594051353 2223 92-93 0.3674613395124524 2223 94-95 0.4260097866701926 2223 96-97 0.502061985835546 2223 98-99 0.3936385280144421 2223 100 0.5619475253813064 2224 1 1.1959496902560431 2224 2 -0.9849548337420408 2224 3 -0.00831029773959635 2224 4 -0.40721510125547766 2224 5 -1.0645515011804214 2224 6 0.4655247581181783 2224 7 0.4928671228601189 2224 8 0.22444880443483584 2224 9 0.14053488049002993 2224 10-11 0.23052265587710252 2224 12-13 0.2664795922653198 2224 14-15 0.29336004502860646 2224 16-17 0.17999557874480843 2224 18-19 0.13669584248808064 2224 20-21 0.15257227043758093 2224 22-23 0.18220935639857316 2224 24-25 0.18249373264195867 2224 26-27 0.1626481795164878 2224 28-29 0.22416965093361085 2224 30-31 0.11336610738445785 2224 32-33 0.04457296737589189 2224 34-35 0.24418673528959545 2224 36-37 0.09254833009455865 2224 38-39 0.18876375242772525 2224 40-41 0.21762461036092162 2224 42-43 0.39822731679649337 2224 44-45 0.40525886618304696 2224 46-47 0.3950793638267882 2224 48-49 0.18081807406477424 2224 50-51 0.22282021325096224 2224 52-53 0.2789036800666338 2224 54-55 0.25933320805524573 2224 56-57 0.27372599989575264 2224 58-59 0.444625730082123 2224 60-61 0.3875226510997578 2224 62-63 0.5357463414297712 2224 64-65 0.3148036021282863 2224 66-67 0.4226258558504057 2224 68-69 0.5290757769102115 2224 70-71 0.5243377419559607 2224 72-73 0.4388361818192408 2224 74-75 0.355064909509899 2224 76-77 0.6623929171184599 2224 78-79 0.45824079654632044 2224 80-81 0.33858836793987734 2224 82-83 0.4399400923758705 2224 84-85 0.3124302632321019 2224 86-87 0.46327400529241203 2224 88-89 0.46330003049077106 2224 90-91 0.43990730413456447 2224 92-93 0.3956549247749521 2224 94-95 0.5088653253726534 2224 96-97 0.38184267562093055 2224 98-99 0.37400101994266777 2224 100 0.6443472172657465 2225 1 1.7913956545865002 2225 2 1.895166466782026 2225 3 0.8736386670327292 2225 4 0.8239212735392272 2225 5 3.1318869687763566 2225 6 -0.3020156318363192 2225 7 0.2545414470282097 2225 8 0.42553799888469257 2225 9 0.09279130938612923 2225 10-11 0.19254863515829612 2225 12-13 0.24884259267551556 2225 14-15 0.21475157616462326 2225 16-17 0.12064469650879062 2225 18-19 0.06617575797841369 2225 20-21 0.07716270893445909 2225 22-23 0.3193158503082003 2225 24-25 0.36700625784361307 2225 26-27 0.25857194580351717 2225 28-29 0.1734620645432159 2225 30-31 0.15200511122825588 2225 32-33 0.31462797943625986 2225 34-35 0.2508521072287735 2225 36-37 0.2628855689911802 2225 38-39 0.10454163024662932 2225 40-41 0.12920917467522486 2225 42-43 0.3244636825637315 2225 44-45 0.3146385333390924 2225 46-47 0.3155819081444733 2225 48-49 0.33818527145092503 2225 50-51 0.25577063191911265 2225 52-53 0.1585506431189927 2225 54-55 0.3950289637247195 2225 56-57 0.45338349498212693 2225 58-59 0.423874950474044 2225 60-61 0.41057998575077903 2225 62-63 0.335464866881658 2225 64-65 0.330705389723299 2225 66-67 0.5584494015190486 2225 68-69 0.4947315464851485 2225 70-71 0.44570036938961266 2225 72-73 0.4190728793851548 2225 74-75 0.37282484882005207 2225 76-77 0.5476483862532646 2225 78-79 0.5058568746069128 2225 80-81 0.5010391524576221 2225 82-83 0.34617212654716667 2225 84-85 0.29311229558207685 2225 86-87 0.23724289540608368 2225 88-89 0.3798543488622812 2225 90-91 0.39755308569859693 2225 92-93 0.5270088890167131 2225 94-95 0.8246779668226196 2225 96-97 0.7069118267389882 2225 98-99 0.46826420913760103 2225 100 0.5871311506082648 2226 1 1.4852442689218837 2226 2 1.5909261604456972 2226 3 0.8139313821509813 2226 4 0.9734140595817138 2226 5 3.1002117829818694 2226 6 -0.32636413285732147 2226 7 0.10564752455402981 2226 8 0.26225315001554605 2226 9 0.1723180427732416 2226 10-11 0.09442689441455343 2226 12-13 0.321180027637169 2226 14-15 0.09237674158600839 2226 16-17 0.010645201432978979 2226 18-19 0.026064522006201685 2226 20-21 0.1281999758620458 2226 22-23 0.1698806021379653 2226 24-25 0.19102902405315092 2226 26-27 0.16218279955986503 2226 28-29 0.19775743110056254 2226 30-31 0.10352974741863363 2226 32-33 0.14191073580563796 2226 34-35 0.09976969068510755 2226 36-37 0.05365555488852891 2226 38-39 0.08472893363443035 2226 40-41 0.11424140175489583 2226 42-43 0.27967808159240093 2226 44-45 0.1678890708485028 2226 46-47 0.10831610783377243 2226 48-49 0.15939925010085432 2226 50-51 0.171575991865474 2226 52-53 0.2170149925359155 2226 54-55 0.35338886428989014 2226 56-57 0.4802122892818872 2226 58-59 0.2877664716227315 2226 60-61 0.3910162285042347 2226 62-63 0.4272785780391857 2226 64-65 0.2877524874073387 2226 66-67 0.2115074807169961 2226 68-69 0.2907110583056536 2226 70-71 0.29155523539095185 2226 72-73 0.3186126351192655 2226 74-75 0.2541101274618782 2226 76-77 0.45399581166832803 2226 78-79 0.3758632624849696 2226 80-81 0.3426781950743987 2226 82-83 0.21637461288779747 2226 84-85 0.18428352007019555 2226 86-87 0.39739427872221 2226 88-89 0.3804344245539397 2226 90-91 0.2971712221056251 2226 92-93 0.27957695690670903 2226 94-95 0.4512988615247906 2226 96-97 0.23070373691415114 2226 98-99 0.5619763765187926 2226 100 0.7044514592461084 2227 1 1.0186908485717936 2227 2 1.6413324462000105 2227 3 0.7523658588440334 2227 4 -0.1246315658415007 2227 5 3.1731622581494037 2227 6 0.11329426021247002 2227 7 0.3327846109714798 2227 8 0.4832235658747024 2227 9 0.39202056688186815 2227 10-11 0.24310296324963332 2227 12-13 0.2123043962208513 2227 14-15 0.2734922372624027 2227 16-17 0.27041026397139944 2227 18-19 0.25019299236369363 2227 20-21 0.26444988830045446 2227 22-23 0.24396178923956313 2227 24-25 0.4251062416962199 2227 26-27 0.24473294316086225 2227 28-29 0.3428773320975935 2227 30-31 0.34926783320563715 2227 32-33 0.17565113321848713 2227 34-35 0.30581280211792006 2227 36-37 0.3593742992943234 2227 38-39 0.2794563641494534 2227 40-41 0.29927673771896934 2227 42-43 0.524095738677886 2227 44-45 0.3897662355654603 2227 46-47 0.3475436979054578 2227 48-49 0.28380519339208377 2227 50-51 0.24252150131822958 2227 52-53 0.3265582323923937 2227 54-55 0.40760738687298925 2227 56-57 0.46762657621925285 2227 58-59 0.4455213147512893 2227 60-61 0.42459434511291505 2227 62-63 0.3788391200648604 2227 64-65 0.3936638673000843 2227 66-67 0.3766762605365841 2227 68-69 0.3187031349551219 2227 70-71 0.3424254029643663 2227 72-73 0.39432188395761614 2227 74-75 0.3845432265193196 2227 76-77 0.6745484614764692 2227 78-79 0.5427895006057852 2227 80-81 0.4514985654015362 2227 82-83 0.3690814333515178 2227 84-85 0.5440186170884331 2227 86-87 0.4638062384672281 2227 88-89 0.46065815413263067 2227 90-91 0.47355294391662994 2227 92-93 0.5490494859187791 2227 94-95 0.4382582173828524 2227 96-97 0.6074335646707851 2227 98-99 0.6225795007161423 2227 100 0.6588489966251814 2228 1 1.0970677512658007 2228 2 1.6527172982311846 2228 3 -0.16426395097463065 2228 4 -0.0966112286894063 2228 5 3.2577996063350696 2228 6 0.3786704153321807 2228 7 0.5329959670555979 2228 8 0.4544282564141682 2228 9 0.36461159677309496 2228 10-11 0.35204371700805126 2228 12-13 0.24157662677119873 2228 14-15 0.27687706580957183 2228 16-17 0.21524485826557793 2228 18-19 0.22292414351068146 2228 20-21 0.25818425675674206 2228 22-23 0.43938857196941683 2228 24-25 0.31084606627067046 2228 26-27 0.3410961507634198 2228 28-29 0.18025956957573186 2228 30-31 0.2921878936378235 2228 32-33 0.19403030603339033 2228 34-35 0.29849802245664847 2228 36-37 0.3732997297366438 2228 38-39 0.45046760516192563 2228 40-41 0.41961633606413073 2228 42-43 0.5177361710967787 2228 44-45 0.48889908852034125 2228 46-47 0.4650484827723602 2228 48-49 0.4495694217662418 2228 50-51 0.34621044778642585 2228 52-53 0.4930268831969187 2228 54-55 0.49692333682073553 2228 56-57 0.6417157880567572 2228 58-59 0.5689738163853804 2228 60-61 0.6159490984721145 2228 62-63 0.4751192529843493 2228 64-65 0.5098468938409297 2228 66-67 0.5256592072317972 2228 68-69 0.6176336340294668 2228 70-71 0.6955346858018601 2228 72-73 0.7280028458626404 2228 74-75 0.6988129912486869 2228 76-77 0.872831878243268 2228 78-79 0.6736705814964523 2228 80-81 0.5401143004705915 2228 82-83 0.4375323302449772 2228 84-85 0.3994701363081319 2228 86-87 0.48432431528343045 2228 88-89 0.4761402808445183 2228 90-91 0.4382306454583613 2228 92-93 0.5924790515037515 2228 94-95 0.8009028886654335 2228 96-97 0.7062146467356527 2228 98-99 0.7481620019979758 2228 100 0.6462660687171748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 17.0 13 27.0 14 129.0 15 423.0 16 1502.0 17 3861.0 18 6395.0 19 8217.0 20 9785.0 21 11429.0 22 12889.0 23 14779.0 24 16879.0 25 19194.0 26 22276.0 27 24889.0 28 28113.0 29 31868.0 30 36685.0 31 42223.0 32 49610.0 33 60050.0 34 73274.0 35 95233.0 36 134341.0 37 221034.0 38 543643.0 39 1036209.0 40 1351.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.68821174195052 19.2088928451047 18.85152835087154 37.251367062073236 2 19.34302389841945 52.65285503774915 11.712910924290615 16.291210139540784 3 40.12655980369665 14.816993007690543 25.484883245389167 19.571563943223644 4 17.71162957716976 16.427877470000897 20.905509062072955 44.95498389075638 5 17.25263084396477 35.5705664668389 26.48439448196064 20.692408207235694 6 16.89018782480325 27.98459896461951 31.349206507535932 23.776006703041304 7 20.316958096017075 26.686044307901007 36.504244262017096 16.492753334064815 8 20.57099538168434 27.826838099607993 34.61933308728916 16.982833431418513 9 20.483815945657486 28.21677156793313 28.175116954106112 23.124295532303275 10-11 19.388427278984167 30.452914127258033 28.783796993606174 21.374861600151615 12-13 24.20420336548532 26.5805911045056 27.398003052277737 21.81720247773134 14-15 21.705864163665925 27.355490608759837 28.747907793282995 22.190737434291243 16-17 20.848872352947044 29.67430401085254 28.286774460614645 21.190049175585767 18-19 21.39676618156065 29.358622684607944 29.99315731200064 19.25145382183077 20-21 22.6986923084596 28.900581528732307 29.97145222586855 18.429273936939545 22-23 22.034871774410743 30.436016877300432 28.60824114989078 18.920870198398056 24-25 22.12328808115468 28.445832044926338 26.651352079239526 22.77952779467946 26-27 20.17114699809482 26.438530517789992 30.01827376736856 23.372048716746633 28-29 19.581389994926823 28.153504831887886 30.726821032522405 21.53828414066289 30-31 20.140180063346385 30.023632690724995 28.391011796393745 21.44517544953488 32-33 20.51372028767219 30.13797093353815 28.947822010313907 20.400486768475755 34-35 21.384158080057457 29.523006792814183 29.79831027500424 19.294524852124127 36-37 21.77610645067978 28.175675540881567 28.873230726262555 21.174987282176094 38-39 21.418583778260427 29.313817786670004 29.022233686621707 20.245364748447866 40-41 21.01231519280755 29.91386287605068 29.805874768507852 19.26794716263392 42-43 20.2925838214485 29.90805859337049 29.596047917977103 20.203309667203907 44-45 20.435159047609545 30.16480304828783 29.025206228242546 20.374831675860076 46-47 19.794802757801776 28.124726311574783 29.088847716618094 22.991623214005347 48-49 20.72714033495257 28.127816623941428 30.376686981935702 20.768356059170298 50-51 21.76389733973048 30.58160853041804 28.30155706063659 19.35293706921489 52-53 20.333360930537836 30.459593203905772 28.356985467578106 20.85006039797829 54-55 22.432562021629877 28.28826427231273 28.303548571247276 20.975625134810112 56-57 21.76490023547007 28.7555600424732 29.263789922870682 20.21574979918605 58-59 21.292031517265723 27.944288717659262 29.156762902028998 21.606916863046024 60-61 19.50852245523317 30.585240511985702 29.50521082702927 20.40102620575186 62-63 20.46246183694244 31.273954788124453 27.57726086312855 20.686322511804562 64-65 19.71702381655365 30.261051643761938 28.922497649944702 21.099426889739714 66-67 20.105912042532392 30.45129821551475 29.52053305138001 19.92225669057285 68-69 20.857131457412745 29.99670832793034 29.355969397424513 19.790190817232403 70-71 19.714681854907084 31.11324349396028 29.134170548512262 20.03790410262037 72-73 19.867395489411788 30.895434550587016 28.59244112395639 20.644728836044806 74-75 19.85837830289368 29.372826748326737 30.512244022622763 20.256550926156823 76-77 19.44640060646564 30.806359909429144 28.570137551993458 21.177101932111757 78-79 19.61491426690473 29.49433932151656 28.50847356188842 22.38227284969028 80-81 20.444073293898366 29.683055299591572 28.57529593681255 21.29757546969751 82-83 20.383529669935065 28.352348558147888 29.740616240910494 21.523505531006553 84-85 20.736915603523087 28.94052048317756 28.503266735160043 21.81929717813931 86-87 21.244192193749814 29.13149731180114 28.955502578476455 20.66880791597259 88-89 21.43365285826858 28.84216931164155 28.465183086790418 21.258994743299453 90-91 21.419229349745144 29.101952061284948 28.436914606046702 21.041903982923206 92-93 21.93245089922496 29.094630584620905 29.218178009635622 19.75474050651851 94-95 21.594585698183597 29.15262386163008 29.0860123886567 20.16677805152963 96-97 21.31241957846648 29.40903514109304 28.70872692089015 20.569818359550336 98-99 22.39861151287243 29.74346902337087 28.27153302145572 19.58638644230098 100 21.824105014313787 30.98764126759299 27.098760136853763 20.089493581239466 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 7.5 2 15.0 3 18.5 4 21.0 5 24.5 6 33.5 7 37.5 8 43.0 9 48.5 10 52.0 11 69.0 12 78.5 13 89.0 14 125.0 15 169.0 16 204.5 17 276.0 18 435.0 19 706.5 20 1151.0 21 1725.5 22 2665.5 23 4230.0 24 6439.0 25 9571.0 26 13746.0 27 18421.0 28 23481.0 29 29703.5 30 36928.0 31 44824.0 32 53809.0 33 64185.0 34 75615.5 35 87547.5 36 98933.0 37 108002.5 38 114430.5 39 119584.5 40 122736.5 41 122430.0 42 120496.5 43 119100.5 44 118168.5 45 114615.0 46 105692.0 47 96046.5 48 90347.0 49 87323.0 50 83841.5 51 77227.5 52 67355.5 53 56307.0 54 46445.0 55 37647.5 56 29434.0 57 21983.5 58 15426.0 59 11006.0 60 8658.0 61 7120.0 62 5899.5 63 4647.0 64 3353.0 65 2519.0 66 2200.5 67 1912.0 68 1582.0 69 1482.5 70 1527.0 71 1390.0 72 977.5 73 518.5 74 275.0 75 174.5 76 123.0 77 93.0 78 82.0 79 80.0 80 64.5 81 48.0 82 32.5 83 26.0 84 25.0 85 22.5 86 16.0 87 14.0 88 18.5 89 15.5 90 13.0 91 11.5 92 9.5 93 13.5 94 17.0 95 16.5 96 11.5 97 10.0 98 37.0 99 83.0 100 90.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.340269119128605 2 0.007022233748616001 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.593438600341137E-4 30-31 0.010473502039839207 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.184353585428864E-4 40-41 0.0012169211893908413 42-43 1.9949527694931824E-5 44-45 0.0 46-47 0.0021345994633577047 48-49 0.0 50-51 0.0 52-53 0.0012967193001705685 54-55 0.019370991391778798 56-57 0.01093234117682264 58-59 0.013306334972519525 60-61 1.9949527694931824E-4 62-63 0.012049514727738821 64-65 3.989905538986365E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.795457492543864E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2506325.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.85291261603216 #Duplication Level Percentage of deduplicated Percentage of total 1 86.36065643140329 64.64346669323004 2 8.464919502407039 12.67247759630841 3 2.319888855231432 5.209513132786358 4 1.0187036409122399 3.050117384593508 5 0.5399776193836734 2.0209448779169583 6 0.330639556720356 1.4849600307955446 7 0.21321280029133485 1.1171719376178761 8 0.1454119984361802 0.87076092898128 9 0.10891258670370453 0.7337181901786579 >10 0.46857410344635314 5.843801411333917 >50 0.020187166485434022 1.0221118820102528 >100 0.00859063100569186 1.1466569835171203 >500 3.25107573312721E-4 0.184298950730142 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.9949527694931824E-5 0.0 0.0 0.0 0.0 12-13 3.989905538986365E-5 0.0 0.0 0.0 0.0 14-15 3.989905538986365E-5 0.0 0.0 0.0 0.0 16-17 3.989905538986365E-5 0.0 0.0 0.0 0.0 18-19 3.989905538986365E-5 0.0 0.0 0.0 0.0 20-21 3.989905538986365E-5 0.0 0.0 0.0 0.0 22-23 3.989905538986365E-5 0.0 1.9949527694931824E-5 0.0 0.0 24-25 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 26-27 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 28-29 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 30-31 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 32-33 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 34-35 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 36-37 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 38-39 3.989905538986365E-5 0.0 3.989905538986365E-5 0.0 0.0 40-41 5.984858308479547E-5 0.0 3.989905538986365E-5 0.0 0.0 42-43 7.97981107797273E-5 0.0 3.989905538986365E-5 0.0 0.0 44-45 7.97981107797273E-5 0.0 3.989905538986365E-5 0.0 0.0 46-47 7.97981107797273E-5 0.0 3.989905538986365E-5 0.0 0.0 48-49 7.97981107797273E-5 0.0 3.989905538986365E-5 0.0 0.0 50-51 1.1969716616959093E-4 0.0 3.989905538986365E-5 0.0 0.0 52-53 1.1969716616959093E-4 0.0 3.989905538986365E-5 0.0 0.0 54-55 1.795457492543864E-4 0.0 3.989905538986365E-5 0.0 0.0 56-57 1.9949527694931821E-4 0.0 3.989905538986365E-5 0.0 0.0 58-59 2.792933877290455E-4 0.0 3.989905538986365E-5 0.0 0.0 60-61 3.191924431189092E-4 0.0 3.989905538986365E-5 0.0 0.0 62-63 3.9899055389863643E-4 0.0 3.989905538986365E-5 0.0 0.0 64-65 3.9899055389863643E-4 0.0 3.989905538986365E-5 0.0 0.0 66-67 4.588391369834319E-4 0.0 3.989905538986365E-5 0.0 0.0 68-69 4.7878866467836374E-4 0.0 3.989905538986365E-5 0.0 0.0 70-71 5.186877200682274E-4 0.0 3.989905538986365E-5 0.0 0.0 72-73 5.58586775458091E-4 0.0 3.989905538986365E-5 0.0 0.0 74-75 5.984858308479547E-4 0.0 3.989905538986365E-5 0.0 0.0 76-77 6.383848862378184E-4 0.0 3.989905538986365E-5 0.0 0.0 78-79 7.181829970175456E-4 0.0 3.989905538986365E-5 0.0 0.0 80-81 0.0010373754401364547 0.0 3.989905538986365E-5 0.0 0.0 82-83 0.0012169211893908413 0.0 3.989905538986365E-5 0.0 0.0 84-85 0.0012967193001705685 0.0 3.989905538986365E-5 0.0 0.0 86-87 0.0013166688278655003 0.0 3.989905538986365E-5 0.0 0.0 88 0.0013166688278655003 0.0 3.989905538986365E-5 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGCA 65 0.0 81.4232 1 TCACCGG 9765 0.0 80.89855 8 CACCGGC 9325 0.0 80.38684 9 CTTCACC 11085 0.0 73.80582 6 CCTTCAC 12390 0.0 70.50241 5 CAGCGTA 940 0.0 70.379105 1 CGGGCCC 740 0.0 68.50479 7 CGGGGCA 1080 0.0 67.8 94 CGTAGTG 2105 0.0 67.11866 4 GTGTGGC 4280 0.0 66.34993 7 TTCACCG 12235 0.0 66.21642 7 GGGCCCG 795 0.0 62.584614 8 ACCTTCA 5080 0.0 61.999428 4 CAGCAGC 60 1.0018994E-8 61.74593 1 GGCCCGA 820 0.0 60.676556 9 TGTGGCA 4765 0.0 60.187626 8 AACGGGC 835 0.0 60.148685 5 GGCTGCC 45 7.9914225E-6 58.80565 1 TGTGTGG 4570 0.0 58.647408 6 CCGCGTA 300 0.0 58.21759 1 >>END_MODULE