FastQCFastQC Report
Tue 7 Mar 2017
1SN_16A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_16A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2616966
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA144180.5509433443155165No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG89790.3431072470945362No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66360.25357608772907253No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT58000.22163069753294465No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA53560.20466448551490543No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG53110.20294493699956362No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50160.1916723411767673No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG49790.19025849017526403No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42640.16293677487594413No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42320.16171398482058993No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA41440.15835131216836595No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38470.14700229196711No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT38220.14604698723636456No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37100.14176722204262493No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC36970.14127046358263728No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30510.11658538934017486No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA29710.1135284142017894No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG28320.10821691989884469No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC26640.10179727210823528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC2450.087.999872
AGCTGCA2450.087.999872
GCTGCCG302.0971129E-887.999863
GCTGCAC600.087.999863
TGCTGCA650.087.999862
GCAGCAA1000.087.999863
CAGCTGC7000.086.114161
AGCAGCA3100.085.161162
GCTGCAA1300.084.615253
AGCAGCC1250.084.4798662
CAGCAGC7100.084.281561
GCTGCCC1800.083.1109853
CAGCCCT159650.082.239781
AGCTGCG750.082.133212
CAGCGTA61950.081.962751
GCTGCAG700.081.7141653
GCTGCAT650.081.2306443
GCAGCAC650.081.2306443
AGCCCTT137250.080.75472
AGCAGCT1200.080.666542
Produced by FastQC (version 0.11.5)