##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_16A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2616966 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.959662066683327 12.0 12.0 12.0 2.0 32.0 2 14.227054153550332 12.0 12.0 12.0 12.0 22.0 3 13.827497949916047 12.0 12.0 12.0 12.0 22.0 4 13.755949446802136 12.0 12.0 12.0 12.0 22.0 5 22.450449107860017 22.0 22.0 22.0 22.0 27.0 6 26.8581471826535 27.0 22.0 32.0 22.0 32.0 7 34.91564659227517 37.0 37.0 37.0 27.0 41.0 8 37.522845921574834 41.0 37.0 41.0 27.0 41.0 9 38.4811514555405 41.0 37.0 41.0 32.0 41.0 10-11 38.86966605603588 41.0 41.0 41.0 37.0 41.0 12-13 39.049128838509944 41.0 41.0 41.0 37.0 41.0 14-15 38.98717006640514 41.0 41.0 41.0 37.0 41.0 16-17 38.864269348550955 41.0 41.0 41.0 37.0 41.0 18-19 38.77086729441651 41.0 41.0 41.0 34.5 41.0 20-21 38.78917322578894 41.0 41.0 41.0 34.5 41.0 22-23 38.78495899449974 41.0 41.0 41.0 34.5 41.0 24-25 38.76770332514828 41.0 41.0 41.0 32.0 41.0 26-27 38.698174909418 41.0 41.0 41.0 32.0 41.0 28-29 38.64052628119739 41.0 41.0 41.0 32.0 41.0 30-31 38.54021890234722 41.0 41.0 41.0 32.0 41.0 32-33 38.64227850113452 41.0 41.0 41.0 32.0 41.0 34-35 38.60616416109342 41.0 41.0 41.0 32.0 41.0 36-37 38.53600180514382 41.0 41.0 41.0 32.0 41.0 38-39 38.42435132898173 41.0 41.0 41.0 32.0 41.0 40-41 38.469984134299025 41.0 41.0 41.0 32.0 41.0 42-43 38.19715426184368 41.0 41.0 41.0 32.0 41.0 44-45 38.34784574961998 41.0 41.0 41.0 32.0 41.0 46-47 38.34870246690251 41.0 41.0 41.0 32.0 41.0 48-49 38.274012921833915 41.0 41.0 41.0 32.0 41.0 50-51 38.14214781544736 41.0 37.0 41.0 32.0 41.0 52-53 38.13592438724844 41.0 37.0 41.0 32.0 41.0 54-55 38.02212466650312 41.0 37.0 41.0 32.0 41.0 56-57 37.7916984783142 41.0 37.0 41.0 27.0 41.0 58-59 37.75842636090801 41.0 37.0 41.0 27.0 41.0 60-61 37.780987792734024 41.0 37.0 41.0 27.0 41.0 62-63 37.76296635111041 41.0 37.0 41.0 27.0 41.0 64-65 37.69882795573194 41.0 37.0 41.0 27.0 41.0 66-67 37.597884917113944 41.0 37.0 41.0 27.0 41.0 68-69 37.58558135642572 41.0 37.0 41.0 27.0 41.0 70-71 37.54792209757406 41.0 37.0 41.0 27.0 41.0 72-73 37.48948075748787 41.0 37.0 41.0 27.0 41.0 74-75 37.29466966708776 41.0 37.0 41.0 27.0 41.0 76-77 36.13929737719175 39.0 34.5 41.0 24.5 41.0 78-79 36.75151740603432 41.0 37.0 41.0 27.0 41.0 80-81 37.50942389010786 41.0 37.0 41.0 27.0 41.0 82-83 37.69994528014502 41.0 37.0 41.0 27.0 41.0 84-85 37.6397641390832 41.0 37.0 41.0 27.0 41.0 86-87 37.516313738886936 41.0 37.0 41.0 27.0 41.0 88-89 37.423539511021545 41.0 37.0 41.0 27.0 41.0 90-91 37.243742562952676 41.0 37.0 41.0 27.0 41.0 92-93 37.14020357925934 41.0 37.0 41.0 27.0 41.0 94-95 36.982349789794746 41.0 37.0 41.0 27.0 41.0 96-97 36.87994895615763 41.0 37.0 41.0 27.0 41.0 98-99 36.7446046299417 41.0 37.0 41.0 27.0 41.0 100 36.606589462759544 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6263623250289285 1101 2 -0.6055438411635414 1101 3 -0.24994862449461408 1101 4 -0.13639188496273214 1101 5 -1.0628434861943674 1101 6 -0.6769942191582494 1101 7 -0.35513544298270716 1101 8 -0.12782636537779268 1101 9 -0.092977798510546 1101 10-11 -0.14764977008708513 1101 12-13 -0.1246343899403044 1101 14-15 -0.07264344160317648 1101 16-17 -0.07557680458661054 1101 18-19 -0.0062856698888182905 1101 20-21 -0.11455437278019787 1101 22-23 0.05715153778899662 1101 24-25 -0.10931510671169065 1101 26-27 -0.07131387418645829 1101 28-29 -0.1496730641294377 1101 30-31 -0.27141365218940194 1101 32-33 -0.3717292107640162 1101 34-35 -0.2356125479509572 1101 36-37 -0.010789996415624614 1101 38-39 -0.040673200288289024 1101 40-41 -0.1356621782470242 1101 42-43 -0.09244765567073188 1101 44-45 -0.08410806701208884 1101 46-47 0.013365428768828735 1101 48-49 -0.09298339400686473 1101 50-51 -0.16596572258994513 1101 52-53 -0.04214398308644718 1101 54-55 -0.2606104157180056 1101 56-57 0.166611580360005 1101 58-59 -0.1306294988895047 1101 60-61 -0.01773323169996388 1101 62-63 -0.1821021410283592 1101 64-65 -0.07834897567480681 1101 66-67 -0.09695856356709243 1101 68-69 0.015358667081564192 1101 70-71 -0.061863153203780996 1101 72-73 -0.029270876370603105 1101 74-75 -0.16048110071449173 1101 76-77 -0.5574256481114261 1101 78-79 -0.09915685173661615 1101 80-81 -0.04884087105123314 1101 82-83 -0.3438917712626264 1101 84-85 -0.23217034794166835 1101 86-87 -0.1817460371957509 1101 88-89 -0.5475007101383156 1101 90-91 -0.06347872476496264 1101 92-93 -0.04925596456080683 1101 94-95 0.1151614726640986 1101 96-97 0.07188514263263102 1101 98-99 -0.37065361505394634 1101 100 -0.2768570934302872 1102 1 1.8694181260596903 1102 2 0.6029761721870024 1102 3 0.04647121215099226 1102 4 -0.03063403509979068 1102 5 -0.8709936061371728 1102 6 -0.30041493504952754 1102 7 0.05305036026942389 1102 8 -0.08203228895597192 1102 9 -0.2944725905706207 1102 10-11 -0.26869553662894674 1102 12-13 -0.2327910958536492 1102 14-15 -0.1343706365639079 1102 16-17 -0.1740428315831366 1102 18-19 -0.18807817758454348 1102 20-21 -0.12150406005822134 1102 22-23 -0.15411997870699423 1102 24-25 -0.2235506262275493 1102 26-27 -0.24249859726499778 1102 28-29 -0.364248356470263 1102 30-31 -0.35363028522544937 1102 32-33 -0.44281034433977595 1102 34-35 -0.1768894113620192 1102 36-37 -0.3931622369590855 1102 38-39 -0.3726850914999673 1102 40-41 -0.4029365836399279 1102 42-43 -0.27906336354535455 1102 44-45 -0.15377582154217606 1102 46-47 -0.34042081592937734 1102 48-49 -0.44418399411156884 1102 50-51 -0.36188439292341457 1102 52-53 -0.270502651321344 1102 54-55 -0.4161740166587933 1102 56-57 -0.1586899696500197 1102 58-59 -0.2011690573169318 1102 60-61 -0.2945092019120281 1102 62-63 -0.4110808021117123 1102 64-65 -0.4267340733722804 1102 66-67 -0.4203671121568533 1102 68-69 -0.7207025169568695 1102 70-71 -0.6310998429387098 1102 72-73 -0.32145271538939113 1102 74-75 -0.4164274507286905 1102 76-77 -0.3657217198590601 1102 78-79 -0.6170463198022915 1102 80-81 -0.39028679297734215 1102 82-83 -0.4343858902141875 1102 84-85 -0.49037797419789086 1102 86-87 -0.3431590590362461 1102 88-89 -0.5327026482740962 1102 90-91 -0.6962547746040144 1102 92-93 -0.4520020166063432 1102 94-95 -0.28996948210442497 1102 96-97 -0.49500292303061144 1102 98-99 -0.43449473911910985 1102 100 -0.8902276926903525 1103 1 1.6426874211366567 1103 2 0.5681571593001191 1103 3 0.4616013429654995 1103 4 0.997037438819989 1103 5 2.8301641845468524 1103 6 -0.2726291577546647 1103 7 -0.4435473253096518 1103 8 -0.4599059019154623 1103 9 -0.40423922408092494 1103 10-11 -0.22439949746330257 1103 12-13 -0.37743679503926586 1103 14-15 -0.32148410113401127 1103 16-17 -0.4375009902621372 1103 18-19 -0.3946377214231731 1103 20-21 -0.20413814749082348 1103 22-23 -0.30480393258258687 1103 24-25 -0.41247940632816693 1103 26-27 -0.412830643734452 1103 28-29 -0.3391813942265358 1103 30-31 -0.3414781538156362 1103 32-33 -0.4796459124555952 1103 34-35 -0.56165033453874 1103 36-37 -0.47777323124179105 1103 38-39 -0.48322641565138014 1103 40-41 -0.43190533317454083 1103 42-43 -0.21908087845846325 1103 44-45 -0.7139069396980204 1103 46-47 -0.7377325784816193 1103 48-49 -0.5519338789862189 1103 50-51 -0.3031163086692601 1103 52-53 -0.20793499523099257 1103 54-55 -0.31250839832105726 1103 56-57 -0.34929036408369996 1103 58-59 -0.45336746322001176 1103 60-61 -0.556524353008129 1103 62-63 -0.38434077498448715 1103 64-65 -0.33799626307294517 1103 66-67 -0.614920353257105 1103 68-69 -0.6065612167105243 1103 70-71 -0.7470119751685687 1103 72-73 -0.5029471343481973 1103 74-75 -0.43144983465612796 1103 76-77 -0.5044136830939792 1103 78-79 -0.5371108364179591 1103 80-81 -0.33641131867152296 1103 82-83 -0.353680648060255 1103 84-85 -0.3978521964659265 1103 86-87 -0.4682708722997333 1103 88-89 -0.32067230507966826 1103 90-91 -0.5081030060252161 1103 92-93 -0.360137979987492 1103 94-95 -0.35015359370319743 1103 96-97 -0.3742109456963263 1103 98-99 -0.5457923802726654 1103 100 -0.43766214279924753 1104 1 1.8091147144665651 1104 2 -0.6651206835242967 1104 3 0.0986584125435197 1104 4 0.06563732404387146 1104 5 -1.1519257023848262 1104 6 -0.5362053569419984 1104 7 -0.39019335484065465 1104 8 -0.13524257596206013 1104 9 -0.13947045333929964 1104 10-11 -0.016892623973973286 1104 12-13 -0.04603066067120665 1104 14-15 -0.16063675798573485 1104 16-17 -0.18684364312900215 1104 18-19 -0.1829555012591939 1104 20-21 -0.04959685578255346 1104 22-23 0.06631727699767964 1104 24-25 -0.13177112152338566 1104 26-27 -0.1796050155813731 1104 28-29 -0.04141429743150127 1104 30-31 -0.19356360586584032 1104 32-33 -0.18773714792972385 1104 34-35 -0.2658880695720782 1104 36-37 -0.2171261501092303 1104 38-39 -0.3059688571209378 1104 40-41 -0.2032699804379945 1104 42-43 -0.12749076766720435 1104 44-45 -0.1103238016328163 1104 46-47 -0.4652546913468498 1104 48-49 -0.40372739044305916 1104 50-51 -0.23096486419797202 1104 52-53 -0.2819065489088288 1104 54-55 -0.2161729066530853 1104 56-57 -0.3639876882308428 1104 58-59 -0.2664197078931494 1104 60-61 -0.22738085439821276 1104 62-63 -0.23405483475114863 1104 64-65 -0.4525379432660728 1104 66-67 -0.8993709779868908 1104 68-69 -0.7381605549785135 1104 70-71 -0.4453479137864278 1104 72-73 -0.25021264335565974 1104 74-75 -0.4040424209805309 1104 76-77 -0.5482049644170175 1104 78-79 -0.579585884920867 1104 80-81 -0.5932423054538063 1104 82-83 -0.2631668518478776 1104 84-85 -0.2672393596957292 1104 86-87 -0.5321175877180409 1104 88-89 -0.4911942671666196 1104 90-91 -0.34123308998410096 1104 92-93 -0.29677994773631866 1104 94-95 -0.30068226003856324 1104 96-97 -0.39546654795554304 1104 98-99 -0.4901088469529711 1104 100 -0.40766418907587365 1105 1 1.4998958176901453 1105 2 0.6213690488149712 1105 3 0.06180452605259923 1105 4 -0.14232442342042617 1105 5 -0.9924559108296336 1105 6 0.4981484998220509 1105 7 -0.11941873207462805 1105 8 -0.09553490999694958 1105 9 -0.024660824211721888 1105 10-11 -0.074524849089606 1105 12-13 -0.07531311349466563 1105 14-15 -5.843197878974138E-4 1105 16-17 -0.029837465885513836 1105 18-19 -0.1172360812898745 1105 20-21 0.018521522557257697 1105 22-23 0.05759615665142803 1105 24-25 -0.22604097015417324 1105 26-27 -0.3200696762788908 1105 28-29 -0.2772843731755614 1105 30-31 -0.04817989143403878 1105 32-33 -0.3153107516586644 1105 34-35 -0.2580149081789571 1105 36-37 -0.238725693504513 1105 38-39 -0.2355191490388009 1105 40-41 -0.19200126737363377 1105 42-43 -0.003896848595630331 1105 44-45 -0.0683650684730921 1105 46-47 -0.26557484973161394 1105 48-49 -0.2757814576733466 1105 50-51 -0.20638966116345614 1105 52-53 -0.2530432449456441 1105 54-55 -0.20636452605403122 1105 56-57 -0.053525465136630146 1105 58-59 0.007844106183114263 1105 60-61 -0.15492681213849124 1105 62-63 -0.45017883741467557 1105 64-65 -0.4725508150005737 1105 66-67 -0.29643277420325376 1105 68-69 -0.22514761784489679 1105 70-71 -0.34989790843558666 1105 72-73 -0.21175127639529734 1105 74-75 -0.28599553576192704 1105 76-77 -0.5223314403560906 1105 78-79 -0.46336051363226005 1105 80-81 -0.6234767612737286 1105 82-83 -0.41618294002965683 1105 84-85 -0.2636565287464876 1105 86-87 -0.2036795266233753 1105 88-89 -0.3738652858469891 1105 90-91 -0.33501720156687753 1105 92-93 -0.22019758647162035 1105 94-95 -0.2891853932506123 1105 96-97 -0.1919459947979547 1105 98-99 -0.3376739190703546 1105 100 -0.605767020262924 1106 1 1.3241754899079634 1106 2 -0.6240270213233678 1106 3 -0.08890258470605872 1106 4 -0.44119436790892763 1106 5 -1.0836864157199315 1106 6 -0.757911505464758 1106 7 -0.20690881930698168 1106 8 -0.3217729045339084 1106 9 -0.24246856057615673 1106 10-11 -0.14292625009409932 1106 12-13 -0.04615746101426765 1106 14-15 -0.009759136952403935 1106 16-17 -0.14207092257773724 1106 18-19 -0.10267092136388811 1106 20-21 -0.13799887571571645 1106 22-23 -0.09315060517781149 1106 24-25 -0.2282691400369714 1106 26-27 -0.2699375060436182 1106 28-29 -0.3109910874235098 1106 30-31 0.04545060464491968 1106 32-33 -0.11727127681506033 1106 34-35 -0.22012941165943545 1106 36-37 -0.24073578249913652 1106 38-39 -0.021134621767679107 1106 40-41 -0.13573419538491294 1106 42-43 -0.02096443164258943 1106 44-45 -0.16467641749061812 1106 46-47 -0.2948505568423698 1106 48-49 -0.15340613826194271 1106 50-51 -0.20014461754728075 1106 52-53 -0.29244539491480026 1106 54-55 -0.3149082303451607 1106 56-57 -0.10428764682664138 1106 58-59 -0.34143005894156886 1106 60-61 -0.07458052612211219 1106 62-63 -0.2253429487901215 1106 64-65 -0.4241864216619504 1106 66-67 -0.5247469428524809 1106 68-69 -0.4255474328065745 1106 70-71 -0.682158460026038 1106 72-73 -0.2623636651520158 1106 74-75 -0.5511907071426663 1106 76-77 -0.42137430756906014 1106 78-79 -0.40378367866883025 1106 80-81 -0.39283639648387947 1106 82-83 -0.37861024640026386 1106 84-85 -0.43787121726529676 1106 86-87 -0.1993476471626323 1106 88-89 -0.29397278430499796 1106 90-91 -0.507868333825229 1106 92-93 -0.340798520474614 1106 94-95 -0.16827275777612272 1106 96-97 -0.2802346105350253 1106 98-99 -0.6295234212291376 1106 100 -0.6903868105528232 1107 1 1.2951755231648043 1107 2 -0.4582749178280725 1107 3 -0.2350747221233327 1107 4 -0.15564280317448365 1107 5 -1.2272811478360666 1107 6 -0.33832987654460567 1107 7 -0.6930639957345335 1107 8 -0.5547886350204578 1107 9 -0.41632203092768094 1107 10-11 -0.2939588750566884 1107 12-13 -0.3890962019102133 1107 14-15 -0.41888897166590056 1107 16-17 -0.3877458701982093 1107 18-19 -0.3046995887622046 1107 20-21 -0.2933257904784341 1107 22-23 -0.3391572898031683 1107 24-25 -0.2306270501769916 1107 26-27 -0.43123984399965565 1107 28-29 -0.4063517578814597 1107 30-31 -0.3623256765749474 1107 32-33 -0.2772175670142758 1107 34-35 -0.3218130393158276 1107 36-37 -0.358211421861931 1107 38-39 -0.44480351664279993 1107 40-41 -0.44614358711726254 1107 42-43 -0.23820394742296713 1107 44-45 -0.08729689040291788 1107 46-47 -0.3009157735107024 1107 48-49 -0.2774342070370963 1107 50-51 -0.4479662943572862 1107 52-53 -0.5047180012535577 1107 54-55 -0.32158703575937864 1107 56-57 -0.29586036291298257 1107 58-59 -0.5493859271114445 1107 60-61 -0.25636991053794844 1107 62-63 -0.4655895482779684 1107 64-65 -0.5756488913150832 1107 66-67 -0.49976440269483646 1107 68-69 -0.5744582712115758 1107 70-71 -0.41470028050559904 1107 72-73 -0.24513163216122535 1107 74-75 -0.6030855157495338 1107 76-77 -0.6074696216799396 1107 78-79 -0.7891332080845075 1107 80-81 -0.652010296513815 1107 82-83 -0.4808284778013743 1107 84-85 -0.3728964924656708 1107 86-87 -0.349235405318872 1107 88-89 -0.23586726034343997 1107 90-91 -0.2707221034883318 1107 92-93 -0.3939945030480274 1107 94-95 -0.23872904165653352 1107 96-97 -0.5020436164880024 1107 98-99 -0.5937656640705953 1107 100 -0.5897649075962832 1108 1 1.528117656405195 1108 2 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0.38373345905160505 2223 58-59 0.5277832674146197 2223 60-61 0.30128943724785273 2223 62-63 0.3456255668212478 2223 64-65 0.35327629419361983 2223 66-67 0.38659855688587896 2223 68-69 0.547228600683745 2223 70-71 0.45389487156301556 2223 72-73 0.44965774597654473 2223 74-75 0.5436756368308266 2223 76-77 0.5930934667201413 2223 78-79 0.6003382906317114 2223 80-81 0.3873212434951867 2223 82-83 0.26912327008324866 2223 84-85 0.2230251284524769 2223 86-87 0.36807778454851103 2223 88-89 0.42477285497635364 2223 90-91 0.41606983118437313 2223 92-93 0.3485046366856821 2223 94-95 0.6629281841391119 2223 96-97 0.4861191745687634 2223 98-99 0.5818177177905781 2223 100 0.7853806759289554 2224 1 1.385502366632542 2224 2 -0.6868408085097624 2224 3 -0.060875776692011385 2224 4 -0.3069770681237838 2224 5 -0.8893092276166712 2224 6 0.6465906703687665 2224 7 0.4090189364761514 2224 8 0.44196229452668234 2224 9 0.30163960216237484 2224 10-11 0.3859932495187124 2224 12-13 0.2970517389261076 2224 14-15 0.21190367610768845 2224 16-17 0.2841103350794896 2224 18-19 0.343557088539157 2224 20-21 0.3253261505506089 2224 22-23 0.4201478820998332 2224 24-25 0.46202013493966376 2224 26-27 0.4277012510591902 2224 28-29 0.36440727006756646 2224 30-31 0.36448823911215555 2224 32-33 0.3859505745884064 2224 34-35 0.43197507561093573 2224 36-37 0.27909711275911064 2224 38-39 0.2536406148110544 2224 40-41 0.44904842121395916 2224 42-43 0.5434561458894294 2224 44-45 0.616893799947313 2224 46-47 0.45244873751136794 2224 48-49 0.3867997024968517 2224 50-51 0.3879002951467214 2224 52-53 0.4781305920265666 2224 54-55 0.41692265085832503 2224 56-57 0.5960555422872744 2224 58-59 0.5157862738064551 2224 60-61 0.5167638554285716 2224 62-63 0.4705168780884108 2224 64-65 0.44858103135857874 2224 66-67 0.509097902221626 2224 68-69 0.5538819425819739 2224 70-71 0.7161285027866597 2224 72-73 0.5493802008878959 2224 74-75 0.5818867794333329 2224 76-77 0.8084147983578589 2224 78-79 0.5373280322961378 2224 80-81 0.3923875671322534 2224 82-83 0.27422051846793494 2224 84-85 0.3169746021168933 2224 86-87 0.47989986344387603 2224 88-89 0.680342655206104 2224 90-91 0.6800704229140422 2224 92-93 0.4856813916922391 2224 94-95 0.6198255964430359 2224 96-97 0.6077245957674435 2224 98-99 0.563668055756473 2224 100 0.7366320612129655 2225 1 1.3073219257398208 2225 2 1.5606954741408163 2225 3 0.7954013040735326 2225 4 1.0494771515349708 2225 5 3.2572375792961346 2225 6 -0.22021067262326 2225 7 0.1514294323816543 2225 8 0.18490185009481053 2225 9 -0.021466090468827304 2225 10-11 -0.06848858205029984 2225 12-13 0.08446367261198162 2225 14-15 0.07616179775369147 2225 16-17 0.00823182951530299 2225 18-19 -0.002946349260795955 2225 20-21 0.14335673230229418 2225 22-23 0.09068603984809442 2225 24-25 0.036290284624747926 2225 26-27 0.0827282425300524 2225 28-29 0.18880234165380472 2225 30-31 0.10000554813847629 2225 32-33 0.08169204318406287 2225 34-35 0.11026219871938281 2225 36-37 0.01913644955979521 2225 38-39 0.04921643950410015 2225 40-41 0.04627555388826465 2225 42-43 0.3167948749786049 2225 44-45 0.10680422604884399 2225 46-47 0.25957104457410907 2225 48-49 -0.06189084764179853 2225 50-51 0.0702173734029401 2225 52-53 0.05320076767509363 2225 54-55 0.15276232728703576 2225 56-57 0.1963998299477865 2225 58-59 0.3056052657791781 2225 60-61 0.259295465746213 2225 62-63 0.3271757956084187 2225 64-65 0.29716743760750575 2225 66-67 0.45777322240425633 2225 68-69 0.49239144843154037 2225 70-71 0.36036017507571927 2225 72-73 0.36171441647082503 2225 74-75 0.16153854286280023 2225 76-77 0.31637680179330374 2225 78-79 0.28413082938634204 2225 80-81 0.2675282175891738 2225 82-83 0.03421354532378729 2225 84-85 0.21609898843124853 2225 86-87 0.20369681476929458 2225 88-89 0.22147686266638544 2225 90-91 0.3369958102716595 2225 92-93 0.42822130596209007 2225 94-95 0.42933692154711167 2225 96-97 0.2882786253055727 2225 98-99 0.363139485628146 2225 100 0.5574829951174962 2226 1 1.401801505113058 2226 2 1.318270757752142 2226 3 0.9849681179077905 2226 4 0.9903799975540934 2226 5 3.184416948650828 2226 6 -0.25342459633845493 2226 7 0.0742318873082084 2226 8 0.5040690893595396 2226 9 0.3636488048832547 2226 10-11 0.20436419318959764 2226 12-13 0.30039480695300824 2226 14-15 0.27403011494102003 2226 16-17 0.2728677775992665 2226 18-19 0.2676769790135367 2226 20-21 0.25345817115044156 2226 22-23 0.32405759432688797 2226 24-25 0.4242929798806898 2226 26-27 0.35504275761230986 2226 28-29 0.40779683068958406 2226 30-31 0.3886997836490522 2226 32-33 0.300611923680961 2226 34-35 0.3720651796810799 2226 36-37 0.42606921881662174 2226 38-39 0.40877197756110917 2226 40-41 0.40038565739457965 2226 42-43 0.3865558364112047 2226 44-45 0.4737814260389186 2226 46-47 0.47752856480921224 2226 48-49 0.44901485327756774 2226 50-51 0.31836699174881034 2226 52-53 0.2552463540316907 2226 54-55 0.5149321305685817 2226 56-57 0.635856978218861 2226 58-59 0.6390838610388272 2226 60-61 0.5288192619149683 2226 62-63 0.4049802766511945 2226 64-65 0.3738721168752832 2226 66-67 0.47962965903572297 2226 68-69 0.5349139109951366 2226 70-71 0.5571365109011523 2226 72-73 0.3916643514445397 2226 74-75 0.637579673561504 2226 76-77 0.7503315253767582 2226 78-79 0.665123288350749 2226 80-81 0.4789249505621527 2226 82-83 0.6250115079173995 2226 84-85 0.5173612570414079 2226 86-87 0.4617174124789827 2226 88-89 0.5107925064050391 2226 90-91 0.5988440058057165 2226 92-93 0.5838279309126975 2226 94-95 0.671299963892757 2226 96-97 0.5230265724920784 2226 98-99 0.6715381958172983 2226 100 0.8277897314955069 2227 1 1.125914639270201 2227 2 1.5828026044366492 2227 3 0.7375851607120776 2227 4 -0.07448199143424183 2227 5 3.2499951828173543 2227 6 0.13280287353484965 2227 7 0.46093106576550014 2227 8 0.4076090698395731 2227 9 0.22396810594539573 2227 10-11 0.3219925605802203 2227 12-13 0.18170012753940767 2227 14-15 0.2403206738234971 2227 16-17 0.2602185583619985 2227 18-19 0.2040821586065107 2227 20-21 0.0939595356145091 2227 22-23 0.3914626027800523 2227 24-25 0.36938950201894727 2227 26-27 0.3503696203331117 2227 28-29 0.3034858617733036 2227 30-31 0.20934239574383895 2227 32-33 0.2158701478798406 2227 34-35 0.14812041058019787 2227 36-37 0.1563754301893212 2227 38-39 0.1602955873365559 2227 40-41 0.35966181117408524 2227 42-43 0.4767105353447647 2227 44-45 0.3336468306628504 2227 46-47 0.340178392702704 2227 48-49 0.3197912265313434 2227 50-51 0.3389971263907299 2227 52-53 0.3955267583799298 2227 54-55 0.3414642973346673 2227 56-57 0.4993205257968043 2227 58-59 0.5724860381663746 2227 60-61 0.5657964709611747 2227 62-63 0.4831561732913414 2227 64-65 0.5715505945759105 2227 66-67 0.3982777404438451 2227 68-69 0.5907056689304042 2227 70-71 0.7231881364071526 2227 72-73 0.45377915260370827 2227 74-75 0.5012997518418558 2227 76-77 0.7192047558877661 2227 78-79 0.4702654847691221 2227 80-81 0.2747814695210806 2227 82-83 0.37547533977360814 2227 84-85 0.25443393560861693 2227 86-87 0.33027013177603237 2227 88-89 0.27626759124098044 2227 90-91 0.4431659412752609 2227 92-93 0.5286785738326998 2227 94-95 0.6193529441959313 2227 96-97 0.6424954984429618 2227 98-99 0.5793471837365445 2227 100 0.5877711567392723 2228 1 1.2852302610517548 2228 2 1.501649148863189 2228 3 -0.3551197997363307 2228 4 -0.021511429679994976 2228 5 3.1181184559662434 2228 6 0.1294331973102132 2228 7 0.43415160394928876 2228 8 0.26777920178377457 2228 9 0.3205499573384998 2228 10-11 0.246715166847018 2228 12-13 0.2100108596199206 2228 14-15 0.2870714228603575 2228 16-17 0.22931489569236163 2228 18-19 0.2950345383593742 2228 20-21 0.29291428464848224 2228 22-23 0.4401851737811171 2228 24-25 0.33718739485576066 2228 26-27 0.3449927664534158 2228 28-29 0.35866359024303307 2228 30-31 0.3139423420535721 2228 32-33 0.19144647262968562 2228 34-35 0.2550278330081923 2228 36-37 0.2578543596370011 2228 38-39 0.23817922627021204 2228 40-41 0.3725957865355767 2228 42-43 0.5417587090479046 2228 44-45 0.44129915161551736 2228 46-47 0.4045834167013922 2228 48-49 0.3656487270837161 2228 50-51 0.22645672374248704 2228 52-53 0.4067090526817836 2228 54-55 0.4302131089156447 2228 56-57 0.5532186121632847 2228 58-59 0.5103752656382312 2228 60-61 0.6174986360609154 2228 62-63 0.5065682079726628 2228 64-65 0.4823860612458404 2228 66-67 0.5489674732951713 2228 68-69 0.6097576182254798 2228 70-71 0.565985219571651 2228 72-73 0.5081009674787893 2228 74-75 0.5982153681536175 2228 76-77 0.5903197682199703 2228 78-79 0.7423305845819286 2228 80-81 0.6128928890673606 2228 82-83 0.5667555648409177 2228 84-85 0.6186900338805188 2228 86-87 0.5195165499656653 2228 88-89 0.6276502596474245 2228 90-91 0.4867956734500609 2228 92-93 0.44112106010965846 2228 94-95 0.7614187673159947 2228 96-97 0.7130117491059949 2228 98-99 0.7408634948551125 2228 100 0.7240224531146069 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 24.0 13 23.0 14 148.0 15 461.0 16 1714.0 17 4232.0 18 6887.0 19 9018.0 20 10707.0 21 12470.0 22 14542.0 23 16489.0 24 19024.0 25 21523.0 26 24454.0 27 27638.0 28 31342.0 29 35540.0 30 40577.0 31 46761.0 32 55023.0 33 65988.0 34 80868.0 35 104855.0 36 148327.0 37 247121.0 38 603652.0 39 986760.0 40 798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.2179124161204 18.45010752966224 26.441177611419263 32.8908024427981 2 26.28282635279731 45.411877101803725 11.906259553653317 16.399036991745643 3 32.208251845839804 15.659507995136352 28.69055234191044 23.441687817113404 4 25.535792211285894 16.84630216823604 20.795073378866977 36.82283224161109 5 16.59478953872538 35.54585730192903 26.332936690809127 21.526416468536468 6 17.127238183453663 28.320734774544263 30.612319762656448 23.939707279345622 7 20.185474324083692 26.570922205332437 36.19427994096981 17.049323529614064 8 21.186480833148007 27.791343104954365 34.001817371719774 17.02035869017786 9 20.834508358152153 28.16360625243125 27.611669391195758 23.39021599822084 10-11 19.652548026990033 30.23428657460586 28.53778000936963 21.575385389034476 12-13 24.46246913410415 26.543676914411574 27.183731084011026 21.81012286747325 14-15 21.746900800392517 27.3151810149616 28.634437742026453 22.30348044261943 16-17 20.903175662198134 29.626273325675612 28.361105952465564 21.109445059660693 18-19 21.45767274011202 29.57185916821235 29.75342438533783 19.217043706337797 20-21 22.77161797287393 28.94091860574421 29.87044921485415 18.417014206527714 22-23 21.950877466501282 30.442600324192213 28.56510172466895 19.041420484637552 24-25 22.339380794400846 28.15351823447458 26.624916028714168 22.88218494241041 26-27 20.16648286603647 26.40055315965129 30.131839695280714 23.30112427903152 28-29 19.605801775535138 28.075904939228092 30.717789646946887 21.600503638289883 30-31 20.242770548278287 30.135404789870634 28.138966132847383 21.4828585290037 32-33 20.677226987282218 30.011203813882183 28.776415131109843 20.535154067725756 34-35 21.41426369314695 29.236680950382997 29.929009394848844 19.420045961621206 36-37 21.96770993585702 27.98009221365505 28.814187880163516 21.238009970324416 38-39 21.597706791259807 29.247983669504645 28.863164656338718 20.291144882896827 40-41 20.989309972582323 29.66582916973165 30.033665466148246 19.311195391537776 42-43 20.50575027125904 29.91157499903992 29.314050425235198 20.268624304465842 44-45 20.585403096562967 30.122324860162493 28.81279313525663 20.47947890801791 46-47 19.87551401054904 27.912783763820155 28.924316018710538 23.28738620692027 48-49 20.831226089912153 28.061202946695325 30.312359868710537 20.79521109468199 50-51 21.881789828373773 30.69682601913819 28.114962135541692 19.306422016946343 52-53 20.337288091551482 30.377250918250837 28.224042504734552 21.06141848546313 54-55 22.411367115695484 28.35182135009055 28.133202391278633 21.10360914293533 56-57 21.64448587101661 28.569181993351876 29.34633149359608 20.440000642035436 58-59 21.178384600568062 27.917336743335667 29.10530242114242 21.798976234953848 60-61 19.517986610802875 30.458980943732243 29.599510577251742 20.423521868213143 62-63 20.529644786893726 31.280472040389196 27.298400372538755 20.89148280017832 64-65 19.757035251535747 30.277202896180462 28.89330919340121 21.072452658882582 66-67 20.274604255462243 30.35778837019663 29.423347494770663 19.944259879570463 68-69 20.87400065572117 29.97866613475299 29.233337383825393 19.913995825700447 70-71 19.717432324302266 30.986359776932527 29.193482070458693 20.102725828306518 72-73 20.049228763384775 30.852674432912007 28.430480182012303 20.66761662169092 74-75 19.946055852464266 29.18966085153571 30.470743601559974 20.39353969444005 76-77 19.418918702038926 30.688151852183026 28.59009249642525 21.3028369493528 78-79 19.521556642310216 29.428735413452067 28.441790990024323 22.60791695421339 80-81 20.618327427632718 29.669735491101708 28.315627695156557 21.396309386109014 82-83 20.350933103448803 28.18351480301999 29.899815282277263 21.56573681125395 84-85 20.644020594841507 28.947223616967126 28.442784506944303 21.96597128124706 86-87 21.325458565376852 29.04632692973466 28.845158859534287 20.7830556453542 88-89 21.235717238970626 28.253653276351315 28.484397581015575 22.026231903662485 90-91 21.38912007263373 28.456961229148565 27.640615124537344 22.51330357368036 92-93 21.811116384393227 28.30686374985384 28.352240724564247 21.52977914118869 94-95 21.733545640256693 28.430862303904597 28.138806541621097 21.69678551421761 96-97 21.420396749518336 28.76090098228254 28.210874730508532 21.607827537690593 98-99 22.364046762548693 29.224204670599462 27.44091822362232 20.970830343229526 100 21.784845504297724 30.46325401247093 26.35594042872548 21.395960054505867 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 10.5 2 14.5 3 16.5 4 26.0 5 24.5 6 29.5 7 33.0 8 42.5 9 52.0 10 61.0 11 80.0 12 102.0 13 120.5 14 130.0 15 159.5 16 211.0 17 306.5 18 458.0 19 696.5 20 1164.5 21 1806.0 22 2749.5 23 4345.0 24 6622.5 25 9738.5 26 14178.5 27 19163.5 28 24399.5 29 30504.5 30 37433.0 31 45694.0 32 55060.0 33 64937.5 34 76452.0 35 89544.5 36 101875.0 37 110006.5 38 114912.5 39 119420.5 40 123011.0 41 124749.0 42 123811.5 43 122364.5 44 122404.5 45 120077.0 46 111135.0 47 99985.5 48 93660.5 49 91777.0 50 89732.5 51 84121.5 52 73772.0 53 62264.5 54 52845.5 55 43929.0 56 34340.0 57 24996.5 58 17572.5 59 12775.0 60 9796.5 61 7974.0 62 6693.0 63 5302.0 64 3953.5 65 3255.5 66 3346.5 67 3077.0 68 2173.5 69 1861.5 70 2158.0 71 2012.0 72 1287.0 73 645.0 74 326.5 75 191.5 76 140.0 77 106.0 78 78.5 79 65.5 80 53.0 81 38.0 82 28.5 83 27.0 84 23.5 85 19.0 86 17.5 87 18.5 88 15.5 89 13.0 90 12.0 91 13.5 92 16.0 93 16.5 94 14.0 95 15.5 96 15.5 97 16.5 98 33.0 99 81.0 100 94.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.336639451945498 2 0.006343223412149795 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.2480360845345334E-4 30-31 0.009801426537448327 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.215620684410879E-4 40-41 0.0015666997584225396 42-43 9.55304730745451E-5 44-45 0.0 46-47 0.0017195485153418118 48-49 1.910609461490902E-5 50-51 0.0 52-53 0.0012992144338138134 54-55 0.02069190046794647 56-57 0.011005110498187596 58-59 0.013374266230436313 60-61 2.1016704076399923E-4 62-63 0.012438067594305774 64-65 7.642437845963608E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1463656768945413E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2616966.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.9683699793636 #Duplication Level Percentage of deduplicated Percentage of total 1 87.41593722421686 67.28262198366347 2 8.19763580026658 12.61917330461989 3 2.060254088917213 4.757231969018302 4 0.8408896177712873 2.5888761284970436 5 0.416694263125973 1.603613910627909 6 0.25070154961654656 1.1577653775171681 7 0.17442224413078464 0.939749707322238 8 0.12212611882093594 0.7519878638042814 9 0.08548340187995956 0.5921566292692219 >10 0.40782699196918554 5.369819827141475 >50 0.01950206693847776 1.0300388281698696 >100 0.008225133680395923 1.1395728607058562 >500 3.014986657878082E-4 0.16739160964311642 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.821218922981804E-5 2 0.0 0.0 0.0 0.0 3.821218922981804E-5 3 0.0 0.0 0.0 0.0 3.821218922981804E-5 4 0.0 0.0 0.0 0.0 3.821218922981804E-5 5 0.0 0.0 0.0 0.0 3.821218922981804E-5 6 0.0 0.0 0.0 0.0 3.821218922981804E-5 7 0.0 0.0 0.0 0.0 3.821218922981804E-5 8 0.0 0.0 0.0 0.0 3.821218922981804E-5 9 0.0 0.0 0.0 0.0 3.821218922981804E-5 10-11 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 12-13 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 14-15 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 16-17 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 18-19 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 20-21 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 22-23 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 24-25 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 26-27 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 28-29 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 30-31 3.821218922981804E-5 0.0 0.0 0.0 3.821218922981804E-5 32-33 7.642437845963608E-5 0.0 0.0 0.0 3.821218922981804E-5 34-35 1.1463656768945413E-4 0.0 0.0 0.0 3.821218922981804E-5 36-37 1.1463656768945413E-4 0.0 0.0 0.0 3.821218922981804E-5 38-39 1.1463656768945413E-4 0.0 0.0 0.0 3.821218922981804E-5 40-41 1.5284875691927216E-4 0.0 0.0 0.0 3.821218922981804E-5 42-43 1.5284875691927216E-4 0.0 0.0 0.0 3.821218922981804E-5 44-45 1.5284875691927216E-4 0.0 0.0 0.0 3.821218922981804E-5 46-47 1.910609461490902E-4 0.0 0.0 0.0 3.821218922981804E-5 48-49 1.910609461490902E-4 0.0 0.0 0.0 3.821218922981804E-5 50-51 1.910609461490902E-4 0.0 0.0 0.0 3.821218922981804E-5 52-53 1.910609461490902E-4 0.0 0.0 0.0 3.821218922981804E-5 54-55 2.2927313537890825E-4 0.0 0.0 0.0 3.821218922981804E-5 56-57 2.2927313537890825E-4 0.0 0.0 0.0 3.821218922981804E-5 58-59 2.6748532460872627E-4 0.0 0.0 0.0 3.821218922981804E-5 60-61 2.6748532460872627E-4 0.0 0.0 0.0 3.821218922981804E-5 62-63 4.0122798691308943E-4 0.0 0.0 0.0 3.821218922981804E-5 64-65 4.967584599876345E-4 0.0 1.910609461490902E-5 0.0 3.821218922981804E-5 66-67 5.731828384472706E-4 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 68-69 7.642437845963608E-4 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 70-71 8.97986446900724E-4 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 72-73 9.93516919975269E-4 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 74-75 0.001050835203819996 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 76-77 0.0011081534876647232 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 78-79 0.0011845778661243593 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 80-81 0.0016431241368821759 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 82-83 0.0017195485153418118 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 84-85 0.0017959728938014478 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 86-87 0.001910609461490902 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 88 0.001910609461490902 0.0 3.821218922981804E-5 0.0 3.821218922981804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10220 0.0 81.15605 8 CACCGGC 10105 0.0 78.317085 9 CTTCACC 11725 0.0 73.98171 6 CGGGCCC 770 0.0 71.323135 7 AGGCAGC 80 0.0 70.40874 2 CGTAGTG 2540 0.0 70.22393 4 CCTTCAC 13060 0.0 69.474266 5 GTGTGGC 4530 0.0 68.906265 7 CGGGGCA 1140 0.0 67.5265 94 TTCACCG 12915 0.0 66.40173 7 GGGCCCG 830 0.0 66.16725 8 GCCTTCA 4640 0.0 65.95761 4 TGTGGCA 5220 0.0 61.77625 8 ACGGGCC 885 0.0 60.994385 6 ACCTTCA 4960 0.0 60.85056 4 TGTGTGG 4945 0.0 59.990993 6 AACGGGC 900 0.0 58.934715 5 GGCAGCA 40 8.098732E-6 58.673946 3 GGCCCGA 960 0.0 57.207096 9 ATCGGAA 3815 0.0 55.859444 94 >>END_MODULE