FastQCFastQC Report
Tue 7 Mar 2017
1SN_16A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_16A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2616966
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG102200.081.156058
CACCGGC101050.078.3170859
CTTCACC117250.073.981716
CGGGCCC7700.071.3231357
AGGCAGC800.070.408742
CGTAGTG25400.070.223934
CCTTCAC130600.069.4742665
GTGTGGC45300.068.9062657
CGGGGCA11400.067.526594
TTCACCG129150.066.401737
GGGCCCG8300.066.167258
GCCTTCA46400.065.957614
TGTGGCA52200.061.776258
ACGGGCC8850.060.9943856
ACCTTCA49600.060.850564
TGTGTGG49450.059.9909936
AACGGGC9000.058.9347155
GGCAGCA408.098732E-658.6739463
GGCCCGA9600.057.2070969
ATCGGAA38150.055.85944494
Produced by FastQC (version 0.11.5)