FastQCFastQC Report
Tue 7 Mar 2017
1SN_17A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_17A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2983401
Sequences flagged as poor quality0
Sequence length94
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA161030.539753120683408No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG84500.28323379927807224No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA68890.2309109636954603No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT65420.21927994258901165No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA63430.21260970281903102No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG55830.1871354202804115No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG49390.16554931770821288No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG49230.16501301702318932No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC43160.14466710978510766No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC43000.1441308091000841No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42200.14144930567496627No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT41670.13967280965582568No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41530.13920354655643008No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41030.1375276069157314No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA39690.13303608867865901No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG37690.1263323301158644No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA36560.12254470652788546No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG35940.12046654137341914No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34710.11634372985730045No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA34590.11594150434353276No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32280.108198663203505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCA450.087.999772
AGCTGCG750.087.999772
AGCAGCC450.087.999772
CTGCAGC850.087.999761
GCAGCCT257.290728E-787.999763
GCAGCTC158.9719566E-487.9997563
CAGCTGC3750.086.826431
CAGCAGC2900.084.9652941
AGCTGCT1000.083.599782
CTGCTGC1700.082.823311
TGCTGCT850.082.823312
GCTGCTT750.082.133123
CAGCCCT169650.081.982681
AGCTGCC1450.081.930822
CAGCGTA64400.081.919041
AGCAGCG600.080.666442
AGCCCTT144150.079.910992
GCCCTTC132850.078.494333
AGCAGCA1350.078.2220152
CAGCTCT64850.078.1617051
Produced by FastQC (version 0.11.5)